BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC135565.4 - phase: 0
(137 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC76834 similar to GP|5738864|emb|CAA63220.1 isocitrate dehydrog... 272 3e-74
TC79518 similar to SP|Q9SS17|RS24_ARATH 40S ribosomal protein S2... 232 3e-62
AJ388998 similar to SP|Q9SS17|RS24_ 40S ribosomal protein S24. [... 197 1e-51
AW329113 weakly similar to GP|18461244|db putative 40S ribosomal... 166 4e-42
BQ149945 weakly similar to GP|2440250|dbj| outer surface protein... 30 0.39
AW685995 similar to PIR|I51618|I516 nucleolar phosphoprotein - A... 28 0.88
BI310995 similar to GP|21593733|gb| similar to extragenic suppre... 27 2.6
TC82723 similar to GP|9294038|dbj|BAB01995.1 gene_id:MOB24.1~unk... 26 4.4
TC85442 similar to GP|13543783|gb|AAH06040.1 Unknown (protein fo... 26 4.4
TC89414 26 5.7
TC79723 homologue to SP|Q01520|EF1A_PODAN Elongation factor 1-al... 26 5.7
TC86116 weakly similar to PIR|G86244|G86244 Serine carboxypeptid... 26 5.7
TC86115 similar to PIR|G86244|G86244 Serine carboxypeptidase hom... 26 5.7
TC86181 PIR|A25642|HSZM4 histone H4 - maize, complete 26 5.7
TC85802 weakly similar to PIR|T47837|T47837 beta-glucosidase-lik... 25 7.4
>TC76834 similar to GP|5738864|emb|CAA63220.1 isocitrate dehydrogenase
(NAD+) {Solanum tuberosum}, partial (42%)
Length = 1547
Score = 272 bits (695), Expect = 3e-74
Identities = 137/137 (100%), Positives = 137/137 (100%)
Frame = -1
Query: 1 MADKAVTIRTRKFMTNRLLSRKQFVIDVLHPGRANVSKAELKEKLARIYDVKDPNTVFVF 60
MADKAVTIRTRKFMTNRLLSRKQFVIDVLHPGRANVSKAELKEKLARIYDVKDPNTVFVF
Sbjct: 698 MADKAVTIRTRKFMTNRLLSRKQFVIDVLHPGRANVSKAELKEKLARIYDVKDPNTVFVF 519
Query: 61 KFRTHFGGGKSTGFGLIYDSVENAKKYEPKYRLVRNGLDTKVEKSRKQMKERKNRAKKIR 120
KFRTHFGGGKSTGFGLIYDSVENAKKYEPKYRLVRNGLDTKVEKSRKQMKERKNRAKKIR
Sbjct: 518 KFRTHFGGGKSTGFGLIYDSVENAKKYEPKYRLVRNGLDTKVEKSRKQMKERKNRAKKIR 339
Query: 121 GVKKTKAGDAAKAGKKK 137
GVKKTKAGDAAKAGKKK
Sbjct: 338 GVKKTKAGDAAKAGKKK 288
>TC79518 similar to SP|Q9SS17|RS24_ARATH 40S ribosomal protein S24.
[Mouse-ear cress] {Arabidopsis thaliana}, partial (96%)
Length = 765
Score = 232 bits (592), Expect = 3e-62
Identities = 117/137 (85%), Positives = 127/137 (92%)
Frame = +3
Query: 1 MADKAVTIRTRKFMTNRLLSRKQFVIDVLHPGRANVSKAELKEKLARIYDVKDPNTVFVF 60
MADKAVTIRTRKF+TNRLLSRKQFV++ HPG+ NVSK+ELKEKLA +Y KD NTVFVF
Sbjct: 117 MADKAVTIRTRKFLTNRLLSRKQFVVEANHPGKPNVSKSELKEKLATMYGAKD-NTVFVF 293
Query: 61 KFRTHFGGGKSTGFGLIYDSVENAKKYEPKYRLVRNGLDTKVEKSRKQMKERKNRAKKIR 120
+T+FGGGKSTGFGLIYDSV+NAKKYEPKYRL+RNGLDTKVEKSRKQMKERKNRAKKIR
Sbjct: 294 GLQTNFGGGKSTGFGLIYDSVDNAKKYEPKYRLIRNGLDTKVEKSRKQMKERKNRAKKIR 473
Query: 121 GVKKTKAGDAAKAGKKK 137
GVKKTKA DAAKAGKKK
Sbjct: 474 GVKKTKASDAAKAGKKK 524
>AJ388998 similar to SP|Q9SS17|RS24_ 40S ribosomal protein S24. [Mouse-ear
cress] {Arabidopsis thaliana}, partial (70%)
Length = 538
Score = 197 bits (501), Expect = 1e-51
Identities = 98/100 (98%), Positives = 99/100 (99%)
Frame = +1
Query: 38 KAELKEKLARIYDVKDPNTVFVFKFRTHFGGGKSTGFGLIYDSVENAKKYEPKYRLVRNG 97
+AELKEKLARIY VKDPNTVFVFKFRTHFGGGKSTGFGLIYDSVENAKKYEPKYRLVRNG
Sbjct: 10 EAELKEKLARIYGVKDPNTVFVFKFRTHFGGGKSTGFGLIYDSVENAKKYEPKYRLVRNG 189
Query: 98 LDTKVEKSRKQMKERKNRAKKIRGVKKTKAGDAAKAGKKK 137
LDTKVEKSRKQMKERKNRAKKIRGVKKTKAGDAAKAGKKK
Sbjct: 190 LDTKVEKSRKQMKERKNRAKKIRGVKKTKAGDAAKAGKKK 309
>AW329113 weakly similar to GP|18461244|db putative 40S ribosomal protein S24
{Oryza sativa (japonica cultivar-group)}, partial (86%)
Length = 498
Score = 166 bits (419), Expect = 4e-42
Identities = 84/131 (64%), Positives = 104/131 (79%), Gaps = 1/131 (0%)
Frame = +1
Query: 3 DKA-VTIRTRKFMTNRLLSRKQFVIDVLHPGRANVSKAELKEKLARIYDVKDPNTVFVFK 61
DKA V IRTRKF+ N LLSR+Q ++DV+HPGRANVSK+EL+EKL +++ V + N +++F
Sbjct: 10 DKAPVQIRTRKFIRNVLLSRRQCIVDVIHPGRANVSKSELQEKLGQLFKVSESNLIYLFG 189
Query: 62 FRTHFGGGKSTGFGLIYDSVENAKKYEPKYRLVRNGLDTKVEKSRKQMKERKNRAKKIRG 121
FRT FGGG+STGF L+YDSVE AKKYEPKYRL RNGL K E SRKQ+KE+KNR KK+ G
Sbjct: 190 FRTAFGGGRSTGFALLYDSVEAAKKYEPKYRLARNGLAKKREGSRKQIKEKKNRQKKVWG 369
Query: 122 VKKTKAGDAAK 132
V + A AK
Sbjct: 370 VGRRIARHKAK 402
>BQ149945 weakly similar to GP|2440250|dbj| outer surface protein A {Borrelia
garinii}, partial (10%)
Length = 648
Score = 29.6 bits (65), Expect = 0.39
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = -1
Query: 104 KSRKQMKERKNRAKKIRGVKKTKAGDAAKAGKKK 137
K +KQ K+++NRA + +G + KA K KKK
Sbjct: 165 KRKKQRKKKQNRATEAKGWNQKKAKKKTKKEKKK 64
>AW685995 similar to PIR|I51618|I516 nucleolar phosphoprotein - African
clawed frog, partial (5%)
Length = 516
Score = 28.5 bits (62), Expect = 0.88
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +3
Query: 104 KSRKQMKERKNRAKKIRGVKKTKAGDAAKAGKKK 137
KS+ K + KK+ V+KTK AAKA ++K
Sbjct: 27 KSKTSKKVKAEEPKKVEKVEKTKGKKAAKAEEEK 128
>BI310995 similar to GP|21593733|gb| similar to extragenic suppressor of
bimD6 mutation {Arabidopsis thaliana}, partial (17%)
Length = 671
Score = 26.9 bits (58), Expect = 2.6
Identities = 16/43 (37%), Positives = 22/43 (50%), Gaps = 4/43 (9%)
Frame = +1
Query: 99 DTKVEKSRKQMKERKNRAKKI----RGVKKTKAGDAAKAGKKK 137
DT ++K + + RK KK+ R +KTK A K KKK
Sbjct: 343 DTPLDKKVARKEARKENKKKVKEEKREARKTKLPKAVKKRKKK 471
>TC82723 similar to GP|9294038|dbj|BAB01995.1 gene_id:MOB24.1~unknown
protein {Arabidopsis thaliana}, partial (30%)
Length = 744
Score = 26.2 bits (56), Expect = 4.4
Identities = 13/40 (32%), Positives = 24/40 (59%), Gaps = 1/40 (2%)
Frame = +2
Query: 74 FGLIYDSVENAKKYE-PKYRLVRNGLDTKVEKSRKQMKER 112
FG IY+ +EN K+ + + VR + ++E +KQ+ +R
Sbjct: 224 FGNIYEKIENTKRQQMMELEKVRLDFNRELELQKKQILDR 343
>TC85442 similar to GP|13543783|gb|AAH06040.1 Unknown (protein for MGC:7642)
{Mus musculus}, partial (62%)
Length = 585
Score = 26.2 bits (56), Expect = 4.4
Identities = 12/39 (30%), Positives = 22/39 (55%)
Frame = +1
Query: 99 DTKVEKSRKQMKERKNRAKKIRGVKKTKAGDAAKAGKKK 137
D + + RK+ + +KN+ KK + KK + K GK++
Sbjct: 49 DQQRSQRRKKSQRKKNQRKKKKRKKKQRRKKRRKKGKRR 165
>TC89414
Length = 795
Score = 25.8 bits (55), Expect = 5.7
Identities = 9/20 (45%), Positives = 16/20 (80%)
Frame = -1
Query: 107 KQMKERKNRAKKIRGVKKTK 126
KQ +++KNR +K+RG K+ +
Sbjct: 120 KQREKKKNRGRKLRGKKRER 61
>TC79723 homologue to SP|Q01520|EF1A_PODAN Elongation factor 1-alpha
(EF-1-alpha). {Podospora anserina}, complete
Length = 1537
Score = 25.8 bits (55), Expect = 5.7
Identities = 17/35 (48%), Positives = 21/35 (59%), Gaps = 1/35 (2%)
Frame = -2
Query: 44 KLARIYDVKDPNTVFV-FKFRTHFGGGKSTGFGLI 77
+LAR+ DV D + V V THFGG S G GL+
Sbjct: 141 RLARV-DVADNDDVDVSLLLLTHFGGVDSKGCGLV 40
>TC86116 weakly similar to PIR|G86244|G86244 Serine carboxypeptidase homolog
30227-33069 [imported] - Arabidopsis thaliana, partial
(15%)
Length = 1329
Score = 25.8 bits (55), Expect = 5.7
Identities = 18/56 (32%), Positives = 24/56 (42%)
Frame = +2
Query: 58 FVFKFRTHFGGGKSTGFGLIYDSVENAKKYEPKYRLVRNGLDTKVEKSRKQMKERK 113
FV+ TH TG GLI SVE +R +R + K E + ER+
Sbjct: 476 FVYAINTHVHADHVTGTGLIKVSVEYGLYEMMSWRAIRLEVAPKNENWGFNISERE 643
>TC86115 similar to PIR|G86244|G86244 Serine carboxypeptidase homolog
30227-33069 [imported] - Arabidopsis thaliana, partial
(15%)
Length = 1232
Score = 25.8 bits (55), Expect = 5.7
Identities = 18/56 (32%), Positives = 24/56 (42%)
Frame = +3
Query: 58 FVFKFRTHFGGGKSTGFGLIYDSVENAKKYEPKYRLVRNGLDTKVEKSRKQMKERK 113
FV+ TH TG GLI SVE +R +R + K E + ER+
Sbjct: 441 FVYAINTHVHADHVTGTGLIKVSVEYGLYEMMSWRAIRLEVAPKNENWGFNISERE 608
>TC86181 PIR|A25642|HSZM4 histone H4 - maize, complete
Length = 612
Score = 25.8 bits (55), Expect = 5.7
Identities = 9/34 (26%), Positives = 22/34 (64%)
Frame = -2
Query: 86 KYEPKYRLVRNGLDTKVEKSRKQMKERKNRAKKI 119
+Y PKY + L++ ++ ++ + K++KN+ K +
Sbjct: 521 RY*PKYHNKNSRLESHIQITKLKEKDKKNKLKSV 420
>TC85802 weakly similar to PIR|T47837|T47837 beta-glucosidase-like protein -
Arabidopsis thaliana, partial (43%)
Length = 1119
Score = 25.4 bits (54), Expect = 7.4
Identities = 20/62 (32%), Positives = 28/62 (44%), Gaps = 3/62 (4%)
Frame = +3
Query: 33 RANVSKAELKEKLARIYDVKDPNTVFVFKFRTHF--GGGKSTGFGLIY-DSVENAKKYEP 89
R + LK L I D + + + F + G + FG+IY D V N K+Y P
Sbjct: 726 RIRYHDSHLKFLLQAIKDGANVKGYYAWSFSDSYEWDAGYTVRFGIIYVDFVNNLKRY-P 902
Query: 90 KY 91
KY
Sbjct: 903 KY 908
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.134 0.367
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,761,116
Number of Sequences: 36976
Number of extensions: 28387
Number of successful extensions: 174
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of query: 137
length of database: 9,014,727
effective HSP length: 86
effective length of query: 51
effective length of database: 5,834,791
effective search space: 297574341
effective search space used: 297574341
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC135565.4