BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC125481.10 + phase: 0 /pseudo
(116 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC79430 similar to PIR|F86358|F86358 Similar to peptide transpor... 225 2e-60
BG646222 similar to PIR|F86358|F86 Similar to peptide transporte... 158 3e-40
AW694006 similar to PIR|F86358|F86 Similar to peptide transporte... 150 9e-38
TC80488 similar to GP|4102839|gb|AAD01600.1| LeOPT1 {Lycopersico... 119 1e-28
TC83116 similar to GP|11933407|dbj|BAB19758. putative nitrate tr... 115 3e-27
BQ138525 similar to PIR|T47573|T47 peptide transport-like protei... 104 4e-24
BG451357 similar to PIR|G86449|G86 hypothetical protein AAF81343... 98 5e-22
TC89683 similar to GP|20466668|gb|AAM20651.1 putative nitrate tr... 97 1e-21
BF639602 similar to GP|4102839|gb| LeOPT1 {Lycopersicon esculent... 95 5e-21
BI267615 similar to PIR|T45958|T459 oligopeptide transporter-lik... 94 1e-20
BE318511 similar to GP|9581817|emb putative low-affinity nitrate... 93 2e-20
TC87099 similar to PIR|G86449|G86449 hypothetical protein AAF813... 92 3e-20
TC85038 weakly similar to PIR|G86449|G86449 hypothetical protein... 89 2e-19
TC79810 similar to GP|11994403|dbj|BAB02362. nitrate transporter... 89 3e-19
BE187570 similar to GP|9581817|emb putative low-affinity nitrate... 86 3e-18
TC78362 similar to GP|15391731|emb|CAA93316. nitrite transporter... 82 3e-17
TC93696 weakly similar to GP|15391731|emb|CAA93316. nitrite tran... 79 3e-16
TC91345 similar to PIR|G96720|G96720 nitrate transporter (NTL1) ... 74 8e-15
TC79437 similar to PIR|G96720|G96720 nitrate transporter (NTL1) ... 73 2e-14
TC78128 weakly similar to GP|21928025|gb|AAM78041.1 At1g69870/T1... 73 2e-14
>TC79430 similar to PIR|F86358|F86358 Similar to peptide transporter
[imported] - Arabidopsis thaliana, partial (55%)
Length = 1303
Score = 225 bits (573), Expect = 2e-60
Identities = 114/114 (100%), Positives = 114/114 (100%)
Frame = +2
Query: 1 MAGGDCETRLLLETVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLIN 60
MAGGDCETRLLLETVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLIN
Sbjct: 41 MAGGDCETRLLLETVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLIN 220
Query: 61 YLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
YLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL
Sbjct: 221 YLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 382
>BG646222 similar to PIR|F86358|F86 Similar to peptide transporter [imported]
- Arabidopsis thaliana, partial (28%)
Length = 749
Score = 158 bits (400), Expect = 3e-40
Identities = 77/103 (74%), Positives = 89/103 (85%)
Frame = +2
Query: 12 LETVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTA 71
L VDG VD+ G+P +R SG WR+A FII VEVAER +YYGI NLI+YLTGPL QSTA
Sbjct: 107 LSLVDGAVDYNGQPAVRFKSGYWRSAWFIIGVEVAERVSYYGIQGNLISYLTGPLKQSTA 286
Query: 72 TAAENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
TAA+NVN+W+GTASLLPLLGAF+ADSFLGRYRTII+ASL+YIL
Sbjct: 287 TAAKNVNVWAGTASLLPLLGAFVADSFLGRYRTIILASLIYIL 415
>AW694006 similar to PIR|F86358|F86 Similar to peptide transporter [imported]
- Arabidopsis thaliana, partial (33%)
Length = 641
Score = 150 bits (378), Expect = 9e-38
Identities = 73/96 (76%), Positives = 85/96 (88%)
Frame = +1
Query: 19 VDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVN 78
VD++G+P +RS SG WR+A FII VEVAER +Y GI NLI+YLTGPL QSTATAA+NVN
Sbjct: 1 VDYRGQPAVRSKSGYWRSAWFIIGVEVAERISYIGIKGNLISYLTGPLKQSTATAAKNVN 180
Query: 79 IWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
IW+GTASLLPLLGAF+ADSFLGRY TII+ASL+YIL
Sbjct: 181 IWAGTASLLPLLGAFVADSFLGRYHTIILASLIYIL 288
>TC80488 similar to GP|4102839|gb|AAD01600.1| LeOPT1 {Lycopersicon
esculentum}, partial (28%)
Length = 664
Score = 119 bits (299), Expect = 1e-28
Identities = 57/101 (56%), Positives = 69/101 (67%)
Frame = +3
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
T DG VDFKGRPVL+ ++G W+A FI+ E ER AYYGI NL+ YLT L Q A+A
Sbjct: 195 TGDGSVDFKGRPVLKQNTGNWKACPFILGNECCERLAYYGIAKNLVTYLTRKLHQGNASA 374
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
A NV W GT L PL+GA LADS+LGRY TI + S +Y +
Sbjct: 375 ARNVTTWQGTCYLTPLIGAILADSYLGRYWTIAVFSTIYFI 497
>TC83116 similar to GP|11933407|dbj|BAB19758. putative nitrate transporter
NRT1-3 {Glycine max}, partial (50%)
Length = 1192
Score = 115 bits (287), Expect = 3e-27
Identities = 54/100 (54%), Positives = 71/100 (71%)
Frame = +3
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
T DG V+ KG+PVLRS +GGW+A +F++ EV ER AYYGI+SNLI T L Q T TA
Sbjct: 81 TEDGTVNLKGKPVLRSKTGGWKACSFVVVYEVFERMAYYGISSNLILLFTKKLHQGTXTA 260
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYI 113
NV W GT + P+LGA++AD+ LGR+ T +IAS +Y+
Sbjct: 261 XNNVTNWVGTIWMTPILGAYVADAHLGRFWTFLIASFIYL 380
>BQ138525 similar to PIR|T47573|T47 peptide transport-like protein -
Arabidopsis thaliana, partial (28%)
Length = 572
Score = 104 bits (260), Expect = 4e-24
Identities = 48/101 (47%), Positives = 69/101 (67%)
Frame = +3
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
T DG VD+ G P R +G W+A FI+ E ER AYYG+++NL+ Y L Q +A A
Sbjct: 111 TKDGTVDYLGNPANRKKTGTWKACPFILGNECCERLAYYGMSTNLVLYFKERLHQHSAVA 290
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
++NV+ W+GT + PL+GAF+ADS+LGRY TI S++Y++
Sbjct: 291 SKNVSNWAGTCYITPLIGAFVADSYLGRYWTIASFSILYVI 413
>BG451357 similar to PIR|G86449|G86 hypothetical protein AAF81343.1
[imported] - Arabidopsis thaliana, partial (20%)
Length = 606
Score = 97.8 bits (242), Expect = 5e-22
Identities = 43/101 (42%), Positives = 67/101 (65%)
Frame = +1
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
T+DG VDF GRP +R+ SG W A I+ + A++GI NL+ +LT +GQ+ A A
Sbjct: 166 TLDGSVDFNGRPAIRAKSGRWSAGTIILLNQCLATLAFFGIGVNLVVFLTRVVGQNNAEA 345
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
A NV+ W+GT + L+GAFL+DS+ GRY+T + +++++
Sbjct: 346 ANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAVFQVIFVI 468
>TC89683 similar to GP|20466668|gb|AAM20651.1 putative nitrate transporter
{Arabidopsis thaliana}, partial (32%)
Length = 661
Score = 96.7 bits (239), Expect = 1e-21
Identities = 51/101 (50%), Positives = 65/101 (63%)
Frame = +1
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
TV VD+KG P RS +GGW AA I+ +E+ ER + GI NL+ YL G + ++TA
Sbjct: 100 TVGDAVDYKGFPADRSKTGGWVPAALILGIEIVERLSTMGIAVNLVTYLMGVMHMPSSTA 279
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
A V + GT+ LL LLG FLADSFLGRY+TI I S + L
Sbjct: 280 ANTVTDFMGTSFLLCLLGGFLADSFLGRYKTIGIFSAIQTL 402
>BF639602 similar to GP|4102839|gb| LeOPT1 {Lycopersicon esculentum}, partial
(32%)
Length = 684
Score = 94.7 bits (234), Expect = 5e-21
Identities = 50/118 (42%), Positives = 71/118 (59%), Gaps = 11/118 (9%)
Frame = +2
Query: 8 TRLLLE-----------TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINS 56
TRLLLE T DG V+ KG ++ +G W++ FI+ ER AYYGI +
Sbjct: 62 TRLLLEDDLLQSEDSVHTGDGSVNVKGELSVKRETGTWKSCPFILGTFFCERLAYYGIAA 241
Query: 57 NLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
NL+ YLT L + T +AA+NV + GT L PL+G+F AD++LGRY TI + +Y++
Sbjct: 242 NLVTYLTTRLHEGTVSAAKNVTTFQGTCYLTPLIGSFFADTYLGRYWTIAVFYGIYLI 415
>BI267615 similar to PIR|T45958|T459 oligopeptide transporter-like protein -
Arabidopsis thaliana, partial (15%)
Length = 644
Score = 93.6 bits (231), Expect = 1e-20
Identities = 42/90 (46%), Positives = 64/90 (70%)
Frame = +2
Query: 26 VLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTAS 85
++R +G W+A FI+ E ER AYYG+++NL+ Y L Q +A A++NV+ W+GT
Sbjct: 143 LIRXKTGTWKACPFILGNECCERLAYYGMSTNLVLYFKERLHQHSAVASKNVSNWAGTCY 322
Query: 86 LLPLLGAFLADSFLGRYRTIIIASLVYILV 115
+ PL+GAF+ADS+LGRY TI S++Y++V
Sbjct: 323 ITPLIGAFVADSYLGRYWTIASFSILYVIV 412
>BE318511 similar to GP|9581817|emb putative low-affinity nitrate transporter
{Nicotiana plumbaginifolia}, partial (24%)
Length = 507
Score = 92.8 bits (229), Expect = 2e-20
Identities = 50/99 (50%), Positives = 63/99 (63%)
Frame = +3
Query: 13 ETVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTAT 72
+TV D+KGRP RS++GGW AAA I+ EV ER GI NL+ YLTG + A
Sbjct: 66 KTVPDASDYKGRPADRSTTGGWSAAAMILGGEVMERLTTLGIAVNLVTYLTGTMHLGNAA 245
Query: 73 AAENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLV 111
+A V + GT+ +L LLG FLAD+FLGRY TI I + V
Sbjct: 246 SANVVTNFLGTSFMLCLLGGFLADTFLGRYLTIAIFAAV 362
>TC87099 similar to PIR|G86449|G86449 hypothetical protein AAF81343.1
[imported] - Arabidopsis thaliana, partial (86%)
Length = 2205
Score = 92.0 bits (227), Expect = 3e-20
Identities = 43/100 (43%), Positives = 65/100 (65%)
Frame = +2
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
T+DG VD+ GRP +R++SG W A I+ + A++G+ NL+ +LT LGQ A A
Sbjct: 143 TLDGSVDWHGRPSIRATSGRWFAGTIILLNQGLATLAFFGVGVNLVLFLTRVLGQDNAAA 322
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYI 113
A NV+ W+GT + L+GAFL+DS+ GRY+T I +++
Sbjct: 323 ANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQGIFV 442
>TC85038 weakly similar to PIR|G86449|G86449 hypothetical protein AAF81343.1
[imported] - Arabidopsis thaliana, partial (14%)
Length = 652
Score = 89.0 bits (219), Expect = 2e-19
Identities = 44/101 (43%), Positives = 63/101 (61%)
Frame = +1
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
T DG VDF G+P L++SSG WR+A ++ + A+ G+ +NL+ + L Q+ A
Sbjct: 247 TKDGSVDFYGKPALKNSSGRWRSATLLLVNQGLVAVAFAGVEANLVIFCKLVLKQTNVEA 426
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
A +IW GT L+GAFL+DS+LGRY T II LV+I+
Sbjct: 427 ANTFSIWMGTTYFFSLIGAFLSDSYLGRYLTCIIFQLVFII 549
>TC79810 similar to GP|11994403|dbj|BAB02362. nitrate transporter
{Arabidopsis thaliana}, partial (60%)
Length = 1236
Score = 88.6 bits (218), Expect = 3e-19
Identities = 44/96 (45%), Positives = 62/96 (63%)
Frame = +3
Query: 16 DGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAE 75
+ VDF+G P ++ SGGW AA I+ E+AER GI+ NL+ YL G L +A++A
Sbjct: 96 NAAVDFRGHPADKTKSGGWLAAGLILGTELAERICVMGISMNLVTYLVGDLHLHSASSAT 275
Query: 76 NVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLV 111
V + GT +LL LLG FLAD+ LGRY T+ I++ +
Sbjct: 276 IVTNFMGTLNLLGLLGGFLADAKLGRYLTVAISATI 383
>BE187570 similar to GP|9581817|emb putative low-affinity nitrate transporter
{Nicotiana plumbaginifolia}, partial (29%)
Length = 614
Score = 85.5 bits (210), Expect = 3e-18
Identities = 44/98 (44%), Positives = 59/98 (59%)
Frame = +3
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
T+ D KG P RS +GGW ++A I+ VE ER GI NL+ YLTG + A++
Sbjct: 27 TIQDAWDCKGHPA*RSKTGGWTSSAMILGVEACERLTTMGIAVNLVTYLTGTMHLGNASS 206
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLV 111
A V + GT +L LLG F+AD+F+GRY TI I + V
Sbjct: 207 ANIVTNFMGTCFMLCLLGGFVADTFIGRYLTIAIFATV 320
>TC78362 similar to GP|15391731|emb|CAA93316. nitrite transporter {Cucumis
sativus}, partial (88%)
Length = 1871
Score = 82.0 bits (201), Expect = 3e-17
Identities = 41/87 (47%), Positives = 58/87 (66%)
Frame = +3
Query: 28 RSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLL 87
R GG R FI++ EV +RFA G ++N+I YLT L +A+ ++ +SG +SL
Sbjct: 75 RQQRGGIRTLPFILANEVCDRFASAGFHANMITYLTQQLNMPLVSASNTLSNFSGLSSLT 254
Query: 88 PLLGAFLADSFLGRYRTIIIASLVYIL 114
PLLGAF+ADSF GR+ TI+ A+L+Y L
Sbjct: 255 PLLGAFIADSFAGRFWTIVFATLIYEL 335
>TC93696 weakly similar to GP|15391731|emb|CAA93316. nitrite transporter
{Cucumis sativus}, partial (28%)
Length = 673
Score = 78.6 bits (192), Expect = 3e-16
Identities = 42/103 (40%), Positives = 60/103 (57%)
Frame = +3
Query: 12 LETVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTA 71
+E V+ V+ + + GG R FI++ EV +RFA G +SNLI+YLT L
Sbjct: 93 MEMVEKEVETDSKKMNHPRRGGIRTLPFILANEVCDRFAGAGFHSNLISYLTQELNMPLV 272
Query: 72 TAAENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
A+ + + GT+S PL+GA LADSF GR+ TI + L+Y L
Sbjct: 273 AASNTLTNFGGTSSFTPLIGALLADSFAGRFWTITVGCLIYEL 401
>TC91345 similar to PIR|G96720|G96720 nitrate transporter (NTL1)
53025-56402 [imported] - Arabidopsis thaliana, partial
(41%)
Length = 1023
Score = 73.9 bits (180), Expect = 8e-15
Identities = 40/98 (40%), Positives = 58/98 (58%)
Frame = +1
Query: 17 GVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAEN 76
G VD++ RP R GG AA+F++ VEV E A+ SNL+ YL + S + +A N
Sbjct: 64 GYVDWRNRPAHRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLRKYMHFSPSKSANN 243
Query: 77 VNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
V + GTA LL LLG FL+D+F Y +I++++ L
Sbjct: 244 VTNFMGTAFLLALLGGFLSDAFFTTYHIYLISAVIEFL 357
>TC79437 similar to PIR|G96720|G96720 nitrate transporter (NTL1)
53025-56402 [imported] - Arabidopsis thaliana, partial
(61%)
Length = 1424
Score = 72.8 bits (177), Expect = 2e-14
Identities = 36/97 (37%), Positives = 60/97 (61%)
Frame = +3
Query: 18 VVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENV 77
++ ++ +P LR GG AA+F++ VE+ E AY SNL+ YL + S + +A NV
Sbjct: 72 MLTWRNKPALRGRHGGMVAASFVLVVEILENLAYLANASNLVLYLKEYMHMSPSKSANNV 251
Query: 78 NIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
+ GT+ LL LLG FL+D+FL Y +I++++ ++
Sbjct: 252 TNFMGTSFLLALLGGFLSDAFLTTYHVYLISAVIELI 362
>TC78128 weakly similar to GP|21928025|gb|AAM78041.1 At1g69870/T17F3_10
{Arabidopsis thaliana}, partial (77%)
Length = 2022
Score = 72.8 bits (177), Expect = 2e-14
Identities = 34/87 (39%), Positives = 52/87 (59%)
Frame = +2
Query: 28 RSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLL 87
+ GGW+A FI+ E ER A +G+ +N + YLT A+ +NIW G ++
Sbjct: 221 KKKPGGWKAMPFILGNESFERLAAFGLLANFMVYLTREFHLEQVHASNILNIWGGISNFA 400
Query: 88 PLLGAFLADSFLGRYRTIIIASLVYIL 114
PLLGAF++D++ GR++TI AS +L
Sbjct: 401 PLLGAFISDTYTGRFKTIAFASFFSLL 481
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.139 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,335,392
Number of Sequences: 36976
Number of extensions: 36787
Number of successful extensions: 185
Number of sequences better than 10.0: 75
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of query: 116
length of database: 9,014,727
effective HSP length: 92
effective length of query: 24
effective length of database: 5,612,935
effective search space: 134710440
effective search space used: 134710440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)
Medicago: description of AC125481.10