BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149547.9 + phase: 0
(180 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17523 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alph... 238 6e-64
TC15863 210 1e-55
TC9187 similar to UP|Q8LAN3 (Q8LAN3) Prolyl 4-hydroxylase alpha ... 177 9e-46
TC11925 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alph... 126 2e-30
BP069281 122 5e-29
TC13277 similar to UP|Q9LSI6 (Q9LSI6) Prolyl 4-hydroxylase alpha... 91 9e-20
TC17036 similar to UP|Q8VZD7 (Q8VZD7) AT3g28480/MFJ20_16, partia... 89 3e-19
AV766573 87 1e-18
BP061999 68 8e-13
TC19415 similar to UP|BAD07294 (BAD07294) Prolyl 4-hydroxylase, ... 65 7e-12
TC16820 similar to UP|Q8L8T9 (Q8L8T9) Prolyl 4-hydroxylase alpha... 38 2e-08
TC15896 similar to UP|BAD07294 (BAD07294) Prolyl 4-hydroxylase, ... 37 0.002
BP038912 31 0.14
BP049323 29 0.42
TC10456 homologue to UP|O23207 (O23207) Minor allergen, partial ... 28 0.93
TC8150 similar to UP|AAR88248 (AAR88248) Mitochondrial citrate s... 28 0.93
BP048527 26 4.6
BP044567 26 4.6
BP076366 26 4.6
TC19720 weakly similar to UP|Q9U1G9 (Q9U1G9) GLY-rich protein, p... 25 6.0
>TC17523 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alpha
subunit-like protein, partial (55%)
Length = 448
Score = 238 bits (606), Expect = 6e-64
Identities = 110/147 (74%), Positives = 123/147 (82%)
Frame = +1
Query: 3 KSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLHYE 62
+S V+D ETG DSR RTSSG FL RG D+IV+NIE++IADFTFIPVEHGE VLHYE
Sbjct: 7 RSTVVDSETGKSKDSRVRTSSGTFLPRGRDKIVRNIEKKIADFTFIPVEHGEGLQVLHYE 186
Query: 63 VGQKYEPHYDYFMDTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVPWWNELS 122
VGQKYEPHYDYF+D F+T GQRIAT+LMYL+DVE GGETVFP+AKGNF SVPWWNELS
Sbjct: 187 VGQKYEPHYDYFLDEFNTKNGGQRIATVLMYLTDVEXGGETVFPSAKGNFXSVPWWNELS 366
Query: 123 DCGKGGLSIKPKMGNAILFWSMKPDAT 149
DCGK GLSI P G+A+LFWSM P AT
Sbjct: 367 DCGKKGLSINPXXGDALLFWSMNPXAT 447
>TC15863
Length = 607
Score = 210 bits (534), Expect = 1e-55
Identities = 92/123 (74%), Positives = 108/123 (87%)
Frame = +2
Query: 58 VLHYEVGQKYEPHYDYFMDTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVPW 117
VLHYEVGQKYEPHYDYF+D F+T GQRIAT+LMYLSDVEEGGET+FP AK NFSSVPW
Sbjct: 11 VLHYEVGQKYEPHYDYFLDEFNTKNGGQRIATVLMYLSDVEEGGETIFPAAKANFSSVPW 190
Query: 118 WNELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMHVGE 177
+N+LS C K GLS+KPK G+A+LFWS++PDATLDPSSLHG CPVI+G+KW KWMH+ E
Sbjct: 191 YNDLSVCAKKGLSVKPKRGDALLFWSIRPDATLDPSSLHGGCPVIRGNKWSSTKWMHLEE 370
Query: 178 FKI 180
+K+
Sbjct: 371 YKV 379
>TC9187 similar to UP|Q8LAN3 (Q8LAN3) Prolyl 4-hydroxylase alpha
subunit-like protein, partial (89%)
Length = 1198
Score = 177 bits (449), Expect = 9e-46
Identities = 86/180 (47%), Positives = 121/180 (66%), Gaps = 2/180 (1%)
Frame = +2
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLH 60
+ +SAV D +G+ S RTSSG F+ + D IV IE +I+ +TF+P E+GE+ VL
Sbjct: 266 LKRSAVADNLSGDSKLSEVRTSSGMFISKKKDPIVAGIEDKISAWTFLPKENGEDMQVLR 445
Query: 61 YEVGQKYEPHYDYFMDTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAK--GNFSSVPWW 118
YE GQKY+PHYDYF D + G R+AT+L+YL++V GGETVFP A+ +
Sbjct: 446 YEHGQKYDPHYDYFTDKVNIVRGGHRMATVLLYLTNVTRGGETVFPVAEEPPRRRGLETN 625
Query: 119 NELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMHVGEF 178
++LS+C K G+++KP+ G+A+LF+S+ AT DP SLH CPVI+G+KW KW+HV F
Sbjct: 626 SDLSECAKKGIAVKPRRGDALLFFSLHTTATPDPDSLHAGCPVIEGEKWSATKWIHVDSF 805
>TC11925 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alpha
subunit-like protein, partial (43%)
Length = 613
Score = 126 bits (316), Expect = 2e-30
Identities = 57/80 (71%), Positives = 65/80 (81%)
Frame = +1
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLH 60
M KS V+D ETG DSR RTSSG FL RG D+IV+NIE+RIAD+TFIP+EHGE VLH
Sbjct: 373 MRKSTVVDSETGKSKDSRARTSSGTFLARGRDKIVRNIEKRIADYTFIPIEHGEGLQVLH 552
Query: 61 YEVGQKYEPHYDYFMDTFST 80
YEVGQKY+ HYDYFMD F+T
Sbjct: 553 YEVGQKYDSHYDYFMDEFNT 612
>BP069281
Length = 468
Score = 122 bits (305), Expect = 5e-29
Identities = 51/67 (76%), Positives = 56/67 (83%)
Frame = -3
Query: 113 SSVPWWNELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKW 172
S+VPWWNELSDCGK GLSIKPK G+A+LFWSMKPD T D SSLHG CPV KGDKW C KW
Sbjct: 466 SAVPWWNELSDCGKKGLSIKPKRGDALLFWSMKPDTTADTSSLHGGCPVTKGDKWSCTKW 287
Query: 173 MHVGEFK 179
M + E+K
Sbjct: 286 MRLNEYK 266
>TC13277 similar to UP|Q9LSI6 (Q9LSI6) Prolyl 4-hydroxylase alpha
subunit-like protein, partial (34%)
Length = 582
Score = 91.3 bits (225), Expect = 9e-20
Identities = 40/70 (57%), Positives = 51/70 (72%)
Frame = +1
Query: 4 SAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLHYEV 63
S V D E+G + S RTSSG FL + D +V +IE RIA +TF+P+E+GE+ +LHYE
Sbjct: 373 SMVADNESGKSIKSEVRTSSGMFLNKAQDEVVADIEARIATWTFLPIENGESIQILHYEN 552
Query: 64 GQKYEPHYDY 73
GQKYEPHYDY
Sbjct: 553 GQKYEPHYDY 582
>TC17036 similar to UP|Q8VZD7 (Q8VZD7) AT3g28480/MFJ20_16, partial (21%)
Length = 697
Score = 89.4 bits (220), Expect = 3e-19
Identities = 39/83 (46%), Positives = 59/83 (70%)
Frame = +2
Query: 97 VEEGGETVFPNAKGNFSSVPWWNELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLH 156
V +GGET+FP+A+ S P S+C G ++KP+ G+A+LF+S+ +AT D +SLH
Sbjct: 2 VGKGGETIFPHAESKLSQ-PKDESWSECAHKGYAVKPRKGDALLFFSLHLNATTDSNSLH 178
Query: 157 GACPVIKGDKWLCAKWMHVGEFK 179
G+CPVI+G+KW KW+HV +F+
Sbjct: 179 GSCPVIEGEKWSATKWIHVSDFE 247
>AV766573
Length = 338
Score = 87.4 bits (215), Expect = 1e-18
Identities = 34/50 (68%), Positives = 44/50 (88%)
Frame = -3
Query: 131 IKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMHVGEFKI 180
+KPK G+A+LFWS++PDATLDPSSLHG CPVI+G+KW KWMH+ E+K+
Sbjct: 336 VKPKRGDALLFWSIRPDATLDPSSLHGGCPVIRGNKWSSTKWMHLEEYKV 187
>BP061999
Length = 564
Score = 68.2 bits (165), Expect = 8e-13
Identities = 27/35 (77%), Positives = 28/35 (79%)
Frame = -2
Query: 141 FWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMHV 175
FWSMKPD T D SSLHG CPVIKGDKW C KWM +
Sbjct: 563 FWSMKPDTTADTSSLHGGCPVIKGDKWSCTKWMRL 459
>TC19415 similar to UP|BAD07294 (BAD07294) Prolyl 4-hydroxylase, partial
(24%)
Length = 443
Score = 65.1 bits (157), Expect = 7e-12
Identities = 24/46 (52%), Positives = 38/46 (82%)
Frame = +3
Query: 128 GLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWM 173
GL ++P+ G+ +LF+S+ P+ T+DP+SL G+CPVIKG+KW+ KW+
Sbjct: 51 GLRVRPRPGDGLLFYSLFPNGTIDPTSLPGSCPVIKGEKWVAPKWI 188
>TC16820 similar to UP|Q8L8T9 (Q8L8T9) Prolyl 4-hydroxylase alpha
subunit-like protein, partial (7%)
Length = 571
Score = 38.1 bits (87), Expect(2) = 2e-08
Identities = 16/25 (64%), Positives = 18/25 (72%)
Frame = +2
Query: 128 GLSIKPKMGNAILFWSMKPDATLDP 152
GLS+KP GNA+LFWSM D DP
Sbjct: 290 GLSVKPTKGNAVLFWSMGLDGQSDP 364
Score = 35.4 bits (80), Expect(2) = 2e-08
Identities = 12/22 (54%), Positives = 15/22 (67%)
Frame = +3
Query: 152 PSSLHGACPVIKGDKWLCAKWM 173
P S+HG C V+ G+KW KWM
Sbjct: 363 PFSVHGGCEVLAGEKWSATKWM 428
>TC15896 similar to UP|BAD07294 (BAD07294) Prolyl 4-hydroxylase, partial
(45%)
Length = 613
Score = 37.0 bits (84), Expect = 0.002
Identities = 21/42 (50%), Positives = 26/42 (61%), Gaps = 2/42 (4%)
Frame = +3
Query: 20 RTSSGAFLKRGSDR--IVKNIERRIADFTFIPVEHGENFNVL 59
RTSSG F+ D+ ++ IE +IA T IP HGE FNVL
Sbjct: 486 RTSSGVFISAFEDKTGVLDVIEEKIARATKIPRTHGEAFNVL 611
>BP038912
Length = 481
Score = 30.8 bits (68), Expect = 0.14
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -2
Query: 153 SSLHGACPVIKGDKWLCAKWMH 174
SS H CPV+KGD W K+ +
Sbjct: 480 SSYHARCPVLKGDMWSAIKFFY 415
>BP049323
Length = 284
Score = 29.3 bits (64), Expect = 0.42
Identities = 18/60 (30%), Positives = 27/60 (45%)
Frame = -3
Query: 103 TVFPNAKGNFSSVPWWNELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVI 162
TV P + S V W CG + KP N + ++P+ LDPS+ CP++
Sbjct: 210 TVAPKKHPDLSRV*VWGVFVLCG-AVCACKPLSRNFLFLVHIEPEDALDPSTCLMICPLL 34
>TC10456 homologue to UP|O23207 (O23207) Minor allergen, partial (27%)
Length = 463
Score = 28.1 bits (61), Expect = 0.93
Identities = 11/24 (45%), Positives = 14/24 (57%)
Frame = -1
Query: 101 GETVFPNAKGNFSSVPWWNELSDC 124
G V+P N +PWW +LSDC
Sbjct: 73 GPNVYPIGTNN---IPWWCQLSDC 11
>TC8150 similar to UP|AAR88248 (AAR88248) Mitochondrial citrate synthase,
partial (98%)
Length = 1956
Score = 28.1 bits (61), Expect = 0.93
Identities = 12/24 (50%), Positives = 16/24 (66%), Gaps = 1/24 (4%)
Frame = +3
Query: 117 WWNELSDC-GKGGLSIKPKMGNAI 139
WWNE +DC G LS P+ GN++
Sbjct: 285 WWNERNDCFGVAWLSC*PR*GNSV 356
>BP048527
Length = 519
Score = 25.8 bits (55), Expect = 4.6
Identities = 11/43 (25%), Positives = 23/43 (52%)
Frame = -3
Query: 114 SVPWWNELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLH 156
+V W +++ +GG+ I+ G++ + WS+K T + H
Sbjct: 265 AVSWRSDIGS-RRGGIYIEENQGSSWILWSIKHGKTTSGTGYH 140
>BP044567
Length = 311
Score = 25.8 bits (55), Expect = 4.6
Identities = 7/11 (63%), Positives = 10/11 (90%)
Frame = -2
Query: 110 GNFSSVPWWNE 120
GN +++PWWNE
Sbjct: 178 GNLAALPWWNE 146
>BP076366
Length = 454
Score = 25.8 bits (55), Expect = 4.6
Identities = 15/58 (25%), Positives = 23/58 (38%), Gaps = 15/58 (25%)
Frame = -3
Query: 117 WWNELSDCGKGGLSIKPKMGNAI---------------LFWSMKPDATLDPSSLHGAC 159
W N L +C G IKP++G + LF++++P L G C
Sbjct: 278 WVNNLFNCNPGYYQIKPRIGCIVVCC*H*CWL*LEISKLFFALQPTKKKIKKVLQGCC 105
>TC19720 weakly similar to UP|Q9U1G9 (Q9U1G9) GLY-rich protein, partial
(16%)
Length = 656
Score = 25.4 bits (54), Expect = 6.0
Identities = 17/57 (29%), Positives = 27/57 (46%), Gaps = 1/57 (1%)
Frame = -1
Query: 44 DFTFIPVEHGENFNVLHYEVGQKYEPHYDYFMDTFSTTYAGQR-IATMLMYLSDVEE 99
DF+ + + G +LHY K + YFMD+ S+ + Q I M +Y E+
Sbjct: 248 DFSSLGI-FGSTSAILHYAPSMK*KNEGLYFMDSISSLSSNQNMILVMCIYHESFED 81
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.137 0.431
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,293,293
Number of Sequences: 28460
Number of extensions: 43539
Number of successful extensions: 243
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 241
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 241
length of query: 180
length of database: 4,897,600
effective HSP length: 84
effective length of query: 96
effective length of database: 2,506,960
effective search space: 240668160
effective search space used: 240668160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)
Medicago: description of AC149547.9