BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149494.4 - phase: 0
(291 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8152 205 6e-54
TC9782 170 2e-43
TC16811 165 1e-41
AV423946 144 1e-35
TC8893 125 1e-29
TC18680 123 3e-29
TC12289 117 2e-27
AV423736 107 3e-24
TC8318 92 1e-19
TC10518 91 2e-19
TC9848 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lect... 86 1e-17
TC14653 weakly similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lec... 80 4e-16
BP065374 49 1e-06
AV423739 35 0.015
BU494440 35 0.020
CB826711 29 0.82
TC16926 weakly similar to UP|Q9LHH2 (Q9LHH2) Ankyrin-like protei... 29 1.1
TC10663 similar to UP|Q8VYH1 (Q8VYH1) AT5g65000/MXK3_23, partial... 29 1.1
BP068691 28 1.8
TC11743 similar to UP|Q94KD1 (Q94KD1) At1g05960/T21E18_20, parti... 27 3.1
>TC8152
Length = 715
Score = 205 bits (522), Expect = 6e-54
Identities = 101/174 (58%), Positives = 127/174 (72%), Gaps = 1/174 (0%)
Frame = +1
Query: 29 GDIPESCISSILMHLDPHDICKLARVNRAFHRASSADFVWESKLPSCYKFLANKFLGEEN 88
G++PESC++ IL + DP IC+LA +NRAF ASSADFVWESKLP+ Y+ + K +
Sbjct: 193 GELPESCVALILGYADPPQICQLATLNRAFRAASSADFVWESKLPANYRAIVRKIFTDFP 372
Query: 89 LSTMSKKDVYTKLCQPNRFDGGTKEVLLDKYSGQVCLFMSSKSLKITGIDDRRYWIYIPT 148
S SK+D Y LC+ N DGGTK+ LDK +G++C+ +S K L ITGIDDRRYW I T
Sbjct: 373 -SDSSKRDTYAALCRLNTLDGGTKKAWLDKSTGKICMCLSFKGLSITGIDDRRYWKDIHT 549
Query: 149 EESRFKNVAYLQQMWWVEVIGELEFVFPVGSYSITFRLQLGKSSK-RLGRRVCN 201
EESRF VAYLQQ WW +V GE+EF FP G+YS+ FRL LG++SK R GRR+CN
Sbjct: 550 EESRFGTVAYLQQTWWFQVDGEVEFPFPAGTYSLFFRLHLGRASKGRFGRRLCN 711
>TC9782
Length = 798
Score = 170 bits (431), Expect = 2e-43
Identities = 81/149 (54%), Positives = 103/149 (68%), Gaps = 11/149 (7%)
Frame = +1
Query: 152 RFKNVAYLQQMWWVEVIGELEFVFPVGSYSITFRLQLGKSSKRLGRRVCNDDQVHGWDIK 211
RF VAYLQQ+WW EV GE+ F P Y+++FRL LG+ SKRLGRRVC+ +Q HGW+IK
Sbjct: 1 RFNCVAYLQQIWWFEVDGEINFPLPADIYTLSFRLHLGRFSKRLGRRVCHYEQTHGWEIK 180
Query: 212 PVRFQLSTSDGQRSISECYL-----------HEPGQWAYYHVGDFMVTKPNKPIKIKFSL 260
PVRF+LSTSDGQR+ EC L H+ G W Y VG+F+VT+ K++FS+
Sbjct: 181 PVRFELSTSDGQRASCECCLDDTEHDEIYGCHKRGCWVDYKVGEFLVTESEPTTKVRFSM 360
Query: 261 AQIDCTHTKGGLCIDSAIIRPTEFRERLK 289
IDCTH+KGGLC+DS I P+ R+R K
Sbjct: 361 KPIDCTHSKGGLCVDSVFIIPSCLRDRKK 447
>TC16811
Length = 509
Score = 165 bits (417), Expect = 1e-41
Identities = 84/125 (67%), Positives = 98/125 (78%), Gaps = 3/125 (2%)
Frame = +2
Query: 1 MGASISSTASIREKNNEE---SNSSSSKTGLGDIPESCISSILMHLDPHDICKLARVNRA 57
MGA ISSTAS N++ S S SKTGL D+PE+CISS+LM LDP +ICKLARVN+A
Sbjct: 146 MGAGISSTAS----ENDDCWGSLSLPSKTGLDDVPENCISSLLMSLDPQEICKLARVNKA 313
Query: 58 FHRASSADFVWESKLPSCYKFLANKFLGEENLSTMSKKDVYTKLCQPNRFDGGTKEVLLD 117
FHRASSADF+WESKLP YKFL NK LGEE L +M+KK++Y KLCQPN FDGG KE+ LD
Sbjct: 314 FHRASSADFLWESKLPPGYKFLVNKVLGEEKLGSMTKKEIYAKLCQPNFFDGGAKEIWLD 493
Query: 118 KYSGQ 122
+ S Q
Sbjct: 494 RCSAQ 508
>AV423946
Length = 426
Score = 144 bits (364), Expect = 1e-35
Identities = 73/92 (79%), Positives = 82/92 (88%)
Frame = +1
Query: 1 MGASISSTASIREKNNEESNSSSSKTGLGDIPESCISSILMHLDPHDICKLARVNRAFHR 60
MGA+ISSTA EKN+E SNS+S ++GLGDIPESCISS+ M+LDP DICKLARVNRAFHR
Sbjct: 157 MGAAISSTAV--EKNDEGSNSTSPRSGLGDIPESCISSLFMNLDPPDICKLARVNRAFHR 330
Query: 61 ASSADFVWESKLPSCYKFLANKFLGEENLSTM 92
ASSADFVWESKLP YKFLANK LGEENL++M
Sbjct: 331 ASSADFVWESKLPPSYKFLANKILGEENLASM 426
>TC8893
Length = 509
Score = 125 bits (313), Expect = 1e-29
Identities = 57/82 (69%), Positives = 67/82 (81%)
Frame = +1
Query: 209 DIKPVRFQLSTSDGQRSISECYLHEPGQWAYYHVGDFMVTKPNKPIKIKFSLAQIDCTHT 268
DIKPVRFQLSTSDGQ S+S+ YL PG+WA+YHVGDF + +PN IKFSLAQIDCTHT
Sbjct: 1 DIKPVRFQLSTSDGQSSLSQSYLRGPGEWAFYHVGDFAIERPNVSTNIKFSLAQIDCTHT 180
Query: 269 KGGLCIDSAIIRPTEFRERLKK 290
KGGLCID A+I PT ++LK+
Sbjct: 181 KGGLCIDGAVICPTGSIDKLKQ 246
>TC18680
Length = 492
Score = 123 bits (309), Expect = 3e-29
Identities = 65/135 (48%), Positives = 89/135 (65%)
Frame = +1
Query: 1 MGASISSTASIREKNNEESNSSSSKTGLGDIPESCISSILMHLDPHDICKLARVNRAFHR 60
MGAS+S N + S+ GLGDIPE+C++ + +HL P +IC LAR+NRAF
Sbjct: 118 MGASLS---------NLGTKGSTIGPGLGDIPENCVARVFLHLTPPEICNLARLNRAFRG 270
Query: 61 ASSADFVWESKLPSCYKFLANKFLGEENLSTMSKKDVYTKLCQPNRFDGGTKEVLLDKYS 120
A+SAD VWE+KLPS YK L + L E +SKKD++ L +P FD G KEV LD+ +
Sbjct: 271 AASADSVWETKLPSNYKDLLHA-LPPERFQNLSKKDIFALLSRPQPFDDGNKEVWLDRVT 447
Query: 121 GQVCLFMSSKSLKIT 135
G+VC+ +S+K+L IT
Sbjct: 448 GRVCMSISAKALSIT 492
>TC12289
Length = 514
Score = 117 bits (294), Expect = 2e-27
Identities = 52/64 (81%), Positives = 59/64 (91%)
Frame = +1
Query: 228 ECYLHEPGQWAYYHVGDFMVTKPNKPIKIKFSLAQIDCTHTKGGLCIDSAIIRPTEFRER 287
ECYL PG+WAYYHVG+F+V +PN+PIK+KFSLAQIDCTHTKGGLCIDSAII PT+FRER
Sbjct: 1 ECYLSGPGEWAYYHVGNFIVEQPNEPIKVKFSLAQIDCTHTKGGLCIDSAIICPTDFRER 180
Query: 288 LKKL 291
LK L
Sbjct: 181 LKHL 192
>AV423736
Length = 454
Score = 107 bits (266), Expect = 3e-24
Identities = 53/150 (35%), Positives = 81/150 (53%)
Frame = +2
Query: 20 NSSSSKTGLGDIPESCISSILMHLDPHDICKLARVNRAFHRASSADFVWESKLPSCYKFL 79
N +S + + +PE C+S IL H P D C+ + V+ A+ +D VW S LPS Y+ +
Sbjct: 5 NPKASCSNMISLPEDCVSEILSHTSPPDACRFSLVSSTLRSAADSDMVWRSFLPSDYEDI 184
Query: 80 ANKFLGEENLSTMSKKDVYTKLCQPNRFDGGTKEVLLDKYSGQVCLFMSSKSLKITGIDD 139
++ + L S K ++ LC P DGG K LDK SG+ +S++ L IT D
Sbjct: 185 VSRAVNPSALQFSSYKQLFHALCSPLLLDGGNKSFKLDKLSGKKSYILSARDLSITWSSD 364
Query: 140 RRYWIYIPTEESRFKNVAYLQQMWWVEVIG 169
+W + ESRF+ VA L+ + W+E+ G
Sbjct: 365 PMFWSWRSNPESRFREVAELRTVNWLEIEG 454
>TC8318
Length = 617
Score = 92.0 bits (227), Expect = 1e-19
Identities = 42/69 (60%), Positives = 50/69 (71%), Gaps = 1/69 (1%)
Frame = +1
Query: 219 TSDGQRSISECYLHEPGQWAYYHVGDFMVTKP-NKPIKIKFSLAQIDCTHTKGGLCIDSA 277
TSD Q SEC+L EPG+W +YH GDF V + KIKFS+ QIDCTHTKGGLCIDS
Sbjct: 7 TSDDQYVTSECFLKEPGKWCFYHAGDFTVLDGGDASTKIKFSMTQIDCTHTKGGLCIDSV 186
Query: 278 IIRPTEFRE 286
+ P+EFR+
Sbjct: 187 FVYPSEFRK 213
>TC10518
Length = 522
Score = 90.9 bits (224), Expect = 2e-19
Identities = 41/76 (53%), Positives = 53/76 (68%)
Frame = +3
Query: 213 VRFQLSTSDGQRSISECYLHEPGQWAYYHVGDFMVTKPNKPIKIKFSLAQIDCTHTKGGL 272
V+FQL+TSDGQ + S +L PG W YH G+F+ N + IKFSL+QIDCTHTKGGL
Sbjct: 3 VKFQLTTSDGQHAESHSHLDNPGHWVLYHAGNFVSKNSNDLMNIKFSLSQIDCTHTKGGL 182
Query: 273 CIDSAIIRPTEFRERL 288
C+DS +I + R+ L
Sbjct: 183 CVDSVLICNSNARKEL 230
>TC9848 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin
PP2-like protein, partial (19%)
Length = 394
Score = 85.5 bits (210), Expect = 1e-17
Identities = 44/118 (37%), Positives = 65/118 (54%), Gaps = 1/118 (0%)
Frame = +1
Query: 31 IPESCISSILMHLDPHDICKLARVNRAFHRASSADFVWESKLPSCYKFLANKFLGEENL- 89
+PE C+S IL H P D C+ + ++ A+++D +W S LPS Y + ++ L L
Sbjct: 40 LPEECVSEILSHTSPPDACRFSMLSSTLRSAANSDMLWRSFLPSDYSDIISRALNPLFLN 219
Query: 90 STMSKKDVYTKLCQPNRFDGGTKEVLLDKYSGQVCLFMSSKSLKITGIDDRRYWIYIP 147
S+ S KD++ LC P DGGT LDK SG+ +S++ L IT D YW + P
Sbjct: 220 SSSSFKDLFKALCNPLLLDGGTMIFKLDKSSGKKSYILSARELSITWSSDPLYWTWKP 393
>TC14653 weakly similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-like
protein, partial (45%)
Length = 643
Score = 80.1 bits (196), Expect = 4e-16
Identities = 49/145 (33%), Positives = 77/145 (52%), Gaps = 5/145 (3%)
Frame = +2
Query: 30 DIPESCISSILMHLDPHDICKLARVNRAFHRASSADFVWESKLPSCYKFL----ANKFLG 85
++PE CI++IL P D+ +LA V+ AF A+ +D VW+ LP + + ++
Sbjct: 110 NLPEGCIANILSLTSPLDVGRLALVSSAFRSAADSDAVWDRFLPDDFNSIISHSSSTAAA 289
Query: 86 EENLSTMSKKDVYTKLCQ-PNRFDGGTKEVLLDKYSGQVCLFMSSKSLKITGIDDRRYWI 144
+ + S SKKD+Y L P G K L +G+ C +S+++L I D RYW
Sbjct: 290 DSSSSFKSKKDLYLSLSHNPLIIGDGKKSFQL--VNGKKCYMLSARALSIVWGDTPRYWR 463
Query: 145 YIPTEESRFKNVAYLQQMWWVEVIG 169
+I E+RF VA L + W+E+ G
Sbjct: 464 WISLPEARFSEVAELVSVCWLEIRG 538
>BP065374
Length = 500
Score = 48.9 bits (115), Expect = 1e-06
Identities = 29/65 (44%), Positives = 36/65 (54%), Gaps = 5/65 (7%)
Frame = -2
Query: 228 ECYLHEPGQWAYYHVGDFMVTKPN-KPIKIKFSLAQIDCTHTKGGLCIDSA----IIRPT 282
ECYL PG+WAYYHVG+F+V +P K KFS A H+ C RPT
Sbjct: 499 ECYLSGPGEWAYYHVGNFIVEQPX*AHSKSKFSSAPKLIVHSITKRCPLHKD*CHPFRPT 320
Query: 283 EFRER 287
+FRE+
Sbjct: 319 DFREK 305
>AV423739
Length = 465
Score = 35.0 bits (79), Expect = 0.015
Identities = 17/65 (26%), Positives = 35/65 (53%)
Frame = +1
Query: 5 ISSTASIREKNNEESNSSSSKTGLGDIPESCISSILMHLDPHDICKLARVNRAFHRASSA 64
+ + S R +N+++ +S + D+P+ + IL L P +C++A V R+ + +
Sbjct: 34 MKKSLSSRVENDDQEDSQD--LSVLDLPDLALECILERLPPSALCQMAGVCRSLRESCVS 207
Query: 65 DFVWE 69
D +WE
Sbjct: 208DHLWE 222
>BU494440
Length = 508
Score = 34.7 bits (78), Expect = 0.020
Identities = 18/66 (27%), Positives = 33/66 (49%)
Frame = +2
Query: 27 GLGDIPESCISSILMHLDPHDICKLARVNRAFHRASSADFVWESKLPSCYKFLANKFLGE 86
GLG ++ + +L H+D + VN+ +H+ + + +WE C K AN GE
Sbjct: 185 GLGCFDDNLLFEVLKHVDAKSLAMAGCVNKQWHKTAQDERLWEL---ICTKQWANTGCGE 355
Query: 87 ENLSTM 92
+ L ++
Sbjct: 356 KQLRSV 373
>CB826711
Length = 444
Score = 29.3 bits (64), Expect = 0.82
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = +1
Query: 12 REKNNEESNSSSSKTGLGDIPESCISSILMHLDPHDICKLARVNR 56
REK EE +++ + G G + +HL PH + K A R
Sbjct: 310 REKEKEEKDNNLHRRGGGRFTPLSTMLLWLHLQPHTVAKQAPARR 444
>TC16926 weakly similar to UP|Q9LHH2 (Q9LHH2) Ankyrin-like protein, partial
(8%)
Length = 801
Score = 28.9 bits (63), Expect = 1.1
Identities = 15/43 (34%), Positives = 21/43 (47%)
Frame = +2
Query: 16 NEESNSSSSKTGLGDIPESCISSILMHLDPHDICKLARVNRAF 58
N+ ++SSSS D PE IL L P +I A ++ F
Sbjct: 131 NDPNSSSSSVVSFSDFPEDVQLCILSFLGPSEIATFACTSKRF 259
>TC10663 similar to UP|Q8VYH1 (Q8VYH1) AT5g65000/MXK3_23, partial (27%)
Length = 584
Score = 28.9 bits (63), Expect = 1.1
Identities = 14/59 (23%), Positives = 31/59 (51%)
Frame = -3
Query: 106 RFDGGTKEVLLDKYSGQVCLFMSSKSLKITGIDDRRYWIYIPTEESRFKNVAYLQQMWW 164
++ GGT E++ YSGQ+ F+ S+++ + +Y + +K+ +Q+ W+
Sbjct: 336 QYHGGTNEIIFPYYSGQIS*FLLLNSVRV-------FLVY*NATDH*WKSRKAVQRRWF 181
>BP068691
Length = 500
Score = 28.1 bits (61), Expect = 1.8
Identities = 11/31 (35%), Positives = 18/31 (57%)
Frame = +3
Query: 124 CLFMSSKSLKITGIDDRRYWIYIPTEESRFK 154
C+F+ K+ K G D ++W+Y P + FK
Sbjct: 30 CIFLRLKTEKYCGSD*SQHWLYCPGTTNIFK 122
>TC11743 similar to UP|Q94KD1 (Q94KD1) At1g05960/T21E18_20, partial (3%)
Length = 416
Score = 27.3 bits (59), Expect = 3.1
Identities = 11/28 (39%), Positives = 16/28 (56%)
Frame = +2
Query: 57 AFHRASSADFVWESKLPSCYKFLANKFL 84
A HR++ F+W+ K SC F + FL
Sbjct: 56 ALHRSTCIHFIWKVKFLSCIFFSFHNFL 139
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.135 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,536,344
Number of Sequences: 28460
Number of extensions: 82896
Number of successful extensions: 423
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 411
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 415
length of query: 291
length of database: 4,897,600
effective HSP length: 89
effective length of query: 202
effective length of database: 2,364,660
effective search space: 477661320
effective search space used: 477661320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC149494.4