BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148917.11 - phase: 0
(473 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15365 homologue to GB|AAP78936.1|32306505|BT009668 At5g19990 {... 62 3e-10
TC9489 homologue to UP|Q9SEI5 (Q9SEI5) 26S proteasome AAA-ATPase... 61 5e-10
TC18707 homologue to PIR|T02901|T02901 MSP1 protein homolog T13J... 59 2e-09
TC9659 homologue to UP|Q9SEA8 (Q9SEA8) Salt-induced AAA-Type ATP... 52 2e-07
TC13255 homologue to UP|Q7XXR9 (Q7XXR9) Katanin, partial (41%) 46 2e-05
AV408284 45 3e-05
AV422700 45 3e-05
CN825587 42 2e-04
CB826675 42 3e-04
TC16349 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulat... 42 3e-04
TC12787 weakly similar to UP|MSP1_YEAST (P28737) MSP1 protein (T... 41 4e-04
TC14911 homologue to UP|Q93X55 (Q93X55) Peroxin 6 (Fragment), pa... 41 5e-04
TC9051 UP|PRS7_ARATH (Q9SSB5) 26S protease regulatory subunit 7 ... 40 0.001
BF177891 39 0.001
TC16655 homologue to UP|O99018 (O99018) Chloroplast protease pre... 38 0.003
TC10924 homologue to UP|Q8H9D4 (Q8H9D4) 26S proteasome AAA-ATPas... 37 0.007
TC12379 homologue to UP|O04327 (O04327) Cell division protein Ft... 37 0.007
TC14829 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulat... 37 0.007
AW719510 36 0.016
AV426828 35 0.020
>TC15365 homologue to GB|AAP78936.1|32306505|BT009668 At5g19990 {Arabidopsis
thaliana;}, partial (87%)
Length = 1385
Score = 61.6 bits (148), Expect = 3e-10
Identities = 48/170 (28%), Positives = 91/170 (53%), Gaps = 10/170 (5%)
Frame = +3
Query: 204 IDEELQRDIVNDLDKFVRAREFYRRTGKAWKRGYLLYGPPGTGKSSLIAAMANYLNYDIY 263
+D+++ ++I ++ ++ E + G A +G LLYGPPGTGK+ L A+A++ +
Sbjct: 342 LDQQI-KEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFI 518
Query: 264 DLDLTD-----VGDNKTLKQLILSMSNR---AILVIEDIDCTINLQNREEEKEVVNNGDN 315
+ ++ +G+ + + + M+ +I+ +++ID +I E GD+
Sbjct: 519 RVSGSELVQKYIGEGSRMVRELFVMAREHAPSIIFMDEID-SIGSARMESGS---GXGDS 686
Query: 316 KV--TLSGLLNATDGLWSCCGEEHIIVFTTNHKERLDPALLRPGRMDKQI 363
+V T+ LLN DG + + ++ TN + LD ALLRPGR+D++I
Sbjct: 687 EVQRTMLELLNQLDGFEA--SNKIKVLMATNRIDILDQALLRPGRIDRKI 830
>TC9489 homologue to UP|Q9SEI5 (Q9SEI5) 26S proteasome AAA-ATPase subunit
RPT2a, partial (69%)
Length = 916
Score = 60.8 bits (146), Expect = 5e-10
Identities = 50/150 (33%), Positives = 79/150 (52%), Gaps = 10/150 (6%)
Frame = +1
Query: 224 EFYRRTGKAWKRGYLLYGPPGTGKSSLIAAMAN-----YLNYDIYDLDLTDVGDNKTLKQ 278
E Y G +G +LYG PGTGK+ L A+AN +L +L +GD L +
Sbjct: 454 ELYEDIGIKPPKGVILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVR 633
Query: 279 LILSMSNR---AILVIEDIDCTINLQNREEEKEVVNNGDNKV--TLSGLLNATDGLWSCC 333
+ +++ +I+ I++ID + + + G+ ++ T+ LLN DG S
Sbjct: 634 ELFRVADDLSPSIVFIDEIDAV-----GTKRYDAHSGGEREIQRTMLELLNQLDGFDSR- 795
Query: 334 GEEHIIVFTTNHKERLDPALLRPGRMDKQI 363
G+ +I+ TN E LDPALLRPGR+D++I
Sbjct: 796 GDVKVIL-ATNRIESLDPALLRPGRIDRKI 882
>TC18707 homologue to PIR|T02901|T02901 MSP1 protein homolog T13J8.110 -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(29%)
Length = 771
Score = 58.5 bits (140), Expect = 2e-09
Identities = 40/161 (24%), Positives = 81/161 (49%), Gaps = 8/161 (4%)
Frame = +3
Query: 235 RGYLLYGPPGTGKSSLIAAMAN-----YLNYDIYDLDLTDVGDNKTLKQLILSMSNR--- 286
RG LL+GPPGTGK+ L A+AN ++N + + G+++ + + S++ +
Sbjct: 306 RGILLFGPPGTGKTMLAXAIANEAGASFINVSMSTITSKWFGEDEKNVRALFSLAAKVAP 485
Query: 287 AILVIEDIDCTINLQNREEEKEVVNNGDNKVTLSGLLNATDGLWSCCGEEHIIVFTTNHK 346
I+ ++++D + + R E E + N+ + DGL + GE+ +++ TN
Sbjct: 486 TIIFVDEVDSMLGQRTRVGEHEAMRKIKNE-----FMTHWDGLLTAPGEQILVLAATNRP 650
Query: 347 ERLDPALLRPGRMDKQIHLSYCNFSGFKQLVVNYLCITEHE 387
LD A++R R +++I + + + ++ L +HE
Sbjct: 651 FDLDEAIIR--RFERRIMVGLPSVENREMILKTLLA*EKHE 767
>TC9659 homologue to UP|Q9SEA8 (Q9SEA8) Salt-induced AAA-Type ATPase,
partial (74%)
Length = 1060
Score = 52.0 bits (123), Expect = 2e-07
Identities = 45/179 (25%), Positives = 85/179 (47%), Gaps = 8/179 (4%)
Frame = +1
Query: 220 VRAREFYRRTGKAWKRGYLLYGPPGTGKSSLIAAMANYLNYDIYDLDLTD-----VGDNK 274
V+ +F+ + W R +LLYGPPGTGKS L A+A + + + +D +G+++
Sbjct: 523 VKFPQFFTGKRRPW-RAFLLYGPPGTGKSYLAKAVATEADSTFFSVSSSDLGSKWMGESE 699
Query: 275 TLKQLILSMSNR---AILVIEDIDCTINLQNREEEKEVVNNGDNKVTLSGLLNATDGLWS 331
L + M+ +I+ +++ID + E E ++ + LL G+ +
Sbjct: 700 KLVSNLFQMARESAPSIIFVDEIDSLCGQRGEGNESEA-----SRRIKTELLVQMQGVGN 864
Query: 332 CCGEEHIIVFTTNHKERLDPALLRPGRMDKQIHLSYCNFSGFKQLVVNYLCITEHELFE 390
++ +++ TN LD A+ R R DK+I++ + + + +L T H L E
Sbjct: 865 -NDQKVLVLAATNTPYALDQAIRR--RFDKRIYIPLPDVKARQHMFKVHLGDTPHNLAE 1032
>TC13255 homologue to UP|Q7XXR9 (Q7XXR9) Katanin, partial (41%)
Length = 532
Score = 45.8 bits (107), Expect = 2e-05
Identities = 41/139 (29%), Positives = 72/139 (51%), Gaps = 8/139 (5%)
Frame = +1
Query: 233 WKRGYLLYGPPGTGKSSLIAAMANYLNYDIYDLDLTDV-----GDNKTLKQLILSMSNR- 286
WK G LL+GPPGTGK+ L A+A +++ + V GD++ L +++ ++
Sbjct: 34 WK-GILLFGPPGTGKTMLAKAVATECKTTFFNISASSVVSKWRGDSEKLVKVLFQLARHH 210
Query: 287 --AILVIEDIDCTINLQNREEEKEVVNNGDNKVTLSGLLNATDGLWSCCGEEHIIVFTTN 344
+ + +++ID I+ Q E E + ++ + LL DGL + E ++ TN
Sbjct: 211 APSTIFLDEIDAIIS-QRGEARSE---HEASRRLKTELLIQMDGL-TRTDELVFVLAATN 375
Query: 345 HKERLDPALLRPGRMDKQI 363
LD A+LR R++K+I
Sbjct: 376 LPWELDAAMLR--RLEKRI 426
>AV408284
Length = 425
Score = 45.1 bits (105), Expect = 3e-05
Identities = 26/86 (30%), Positives = 49/86 (56%), Gaps = 8/86 (9%)
Frame = +1
Query: 234 KRGYLLYGPPGTGKSSLIAAMAN-----YLNYDIYDLDLTDVGDNKTLKQLILSMSNR-- 286
++G LLYGPPGTGK+ L A+A ++N I +L GD + L + S++++
Sbjct: 91 QKGVLLYGPPGTGKTMLAKAIAKESGAVFINVRISNLMSKWFGDAQKLVAAVFSLAHKLQ 270
Query: 287 -AILVIEDIDCTINLQNREEEKEVVN 311
AI+ I+++D + + + + ++N
Sbjct: 271 PAIIFIDEVDSFLGQRRTTDHEALLN 348
>AV422700
Length = 472
Score = 44.7 bits (104), Expect = 3e-05
Identities = 28/74 (37%), Positives = 39/74 (51%)
Frame = +2
Query: 181 SNEYGSWRHDVKFNHPMSFNTLAIDEELQRDIVNDLDKFVRAREFYRRTGKAWKRGYLLY 240
SN WR + + NT A+ E R+++ +F R+ G W RG LLY
Sbjct: 176 SNSNNQWRAE----EAIGGNTEAL--EALRELIIFPQRFSHEA---RKLGLKWPRGLLLY 328
Query: 241 GPPGTGKSSLIAAM 254
GPPGTGK+SL+ A+
Sbjct: 329 GPPGTGKTSLVRAV 370
>CN825587
Length = 663
Score = 42.4 bits (98), Expect = 2e-04
Identities = 43/157 (27%), Positives = 70/157 (44%), Gaps = 9/157 (5%)
Frame = +2
Query: 312 NGDNKVTLSGLLNATDGLWSCCGEEHIIVFTTNHKERLDPALLRPGRMDKQIHLSYCNFS 371
N + + T++ LL DG G I++ TN + LD ALLRPGR D+Q+ + + +
Sbjct: 86 NDEREQTINQLLTEMDGFSGNSGV--IVLAATNRPDVLDSALLRPGRFDRQVTVDRPDVA 259
Query: 372 G---FKQLVVNYLCITEHELFEKIEVLLGEVQVTPAEIGEELTKDCDATECLQDLIKFLQ 428
G Q+ + + F+KI + TP G +L + L + L
Sbjct: 260 GRVKILQVHSRGKALAKDVDFDKI------ARRTPGFTGADL-------QNLMNEAAILA 400
Query: 429 AKKMIKEEVKNEEN------IQEPEPKKMIEEETKNE 459
A++ +KE K+E + I PE K + E K +
Sbjct: 401 ARRDLKEISKDEISDALERIIAGPEKKNAVVSEEKKK 511
>CB826675
Length = 551
Score = 41.6 bits (96), Expect = 3e-04
Identities = 33/117 (28%), Positives = 54/117 (45%), Gaps = 8/117 (6%)
Frame = +1
Query: 223 REFYRRTGKAWKRGYLLYGPPGTGKSSLIAAMANYLNYDIYDLDLTD-----VGDNKTLK 277
+E +++ G +G LLYGPPGTGK+ + A A N L +GD L
Sbjct: 205 KERFQKLGVRPPKGVLLYGPPGTGKTLMARACAAQTNATFLKLAGPQLVQMFIGDGAKLV 384
Query: 278 QLILSMSNR---AILVIEDIDCTINLQNREEEKEVVNNGDNKVTLSGLLNATDGLWS 331
+ ++ I+ I++ID + + + EV + + + T+ LLN DG S
Sbjct: 385 RDAFQLAKEKSPCIIFIDEIDA---IGTKRFDSEVSGDREVQRTMLELLNQLDGFSS 546
>TC16349 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulatory subunit
6B homolog, partial (65%)
Length = 901
Score = 41.6 bits (96), Expect = 3e-04
Identities = 19/34 (55%), Positives = 24/34 (69%)
Frame = +3
Query: 224 EFYRRTGKAWKRGYLLYGPPGTGKSSLIAAMANY 257
E Y++ G RG LLYGPPGTGK+ L A+AN+
Sbjct: 570 ELYKQIGIDPPRGVLLYGPPGTGKTMLAKAVANH 671
>TC12787 weakly similar to UP|MSP1_YEAST (P28737) MSP1 protein (TAT-binding
homolog 4), partial (20%)
Length = 389
Score = 41.2 bits (95), Expect = 4e-04
Identities = 21/90 (23%), Positives = 48/90 (53%), Gaps = 8/90 (8%)
Frame = +3
Query: 235 RGYLLYGPPGTGKSSLIAAM-----ANYLNYDIYDLDLTDVGDNKTLKQLILSMSNR--- 286
+G LL+GPPGTGK+ L A+ AN++N + + G+ + + + S++++
Sbjct: 84 KGILLFGPPGTGKTMLAKAVATEAGANFINISMSSITSKWFGEGEKYVKAVFSLASKISP 263
Query: 287 AILVIEDIDCTINLQNREEEKEVVNNGDNK 316
+++ ++++D + + E E + N+
Sbjct: 264 SVIFVDEVDXMLXRRENPGEHEAMRKMXNE 353
>TC14911 homologue to UP|Q93X55 (Q93X55) Peroxin 6 (Fragment), partial (32%)
Length = 1418
Score = 40.8 bits (94), Expect = 5e-04
Identities = 22/63 (34%), Positives = 38/63 (59%)
Frame = +3
Query: 319 LSGLLNATDGLWSCCGEEHIIVFTTNHKERLDPALLRPGRMDKQIHLSYCNFSGFKQLVV 378
LS +L DGL S ++ I+ +N + +DPALLRPGR DK +++ + + +++ V+
Sbjct: 597 LSQMLAEIDGL-SDSSQDLFIIGASNRPDLIDPALLRPGRFDKLLYVGVNSDASYRERVL 773
Query: 379 NYL 381
L
Sbjct: 774 KAL 782
Score = 33.9 bits (76), Expect = 0.059
Identities = 17/50 (34%), Positives = 32/50 (64%)
Frame = +2
Query: 206 EELQRDIVNDLDKFVRAREFYRRTGKAWKRGYLLYGPPGTGKSSLIAAMA 255
E++++ I++ + + ++ + +G + G LLYGPPGTGK+ L A+A
Sbjct: 242 EDVKKSILDTVQLPLLHKDLFA-SGLRKRSGVLLYGPPGTGKTLLAKAVA 388
>TC9051 UP|PRS7_ARATH (Q9SSB5) 26S protease regulatory subunit 7 (26S
proteasome subunit 7) (26S proteasome AAA-ATPase subunit
RPT1a) (Regulatory particle triple-A ATPase subunit 1a),
partial (46%)
Length = 867
Score = 39.7 bits (91), Expect = 0.001
Identities = 22/53 (41%), Positives = 35/53 (65%), Gaps = 2/53 (3%)
Frame = +2
Query: 313 GDNKV--TLSGLLNATDGLWSCCGEEHIIVFTTNHKERLDPALLRPGRMDKQI 363
GDN+V T+ ++N DG + G +++ TN + LDPALLRPGR+D+++
Sbjct: 161 GDNEVQRTMLEIVNQLDG-FDARGNIKVLM-ATNRPDTLDPALLRPGRLDRKV 313
>BF177891
Length = 390
Score = 39.3 bits (90), Expect = 0.001
Identities = 28/105 (26%), Positives = 51/105 (47%), Gaps = 8/105 (7%)
Frame = +1
Query: 235 RGYLLYGPPGTGKSSLIAAM-----ANYLNYDIYDLDLTDVGDNKTLKQLILSMSNR--- 286
+G LL+GPPGTGK+ + A+ AN+++ L GD + L + + S +++
Sbjct: 82 KGILLFGPPGTGKTLMAKALATEAGANFISVSGSTLTSKWFGDAEKLTKALFSFASKLAP 261
Query: 287 AILVIEDIDCTINLQNREEEKEVVNNGDNKVTLSGLLNATDGLWS 331
I+ ++++D + + E E N+ + A DGL S
Sbjct: 262 VIIFVDEVDSLLGARGGAFEHEATRRMRNE-----FMAAWDGLRS 381
>TC16655 homologue to UP|O99018 (O99018) Chloroplast protease precursor,
partial (39%)
Length = 934
Score = 38.1 bits (87), Expect = 0.003
Identities = 21/62 (33%), Positives = 38/62 (60%)
Frame = +3
Query: 194 NHPMSFNTLAIDEELQRDIVNDLDKFVRAREFYRRTGKAWKRGYLLYGPPGTGKSSLIAA 253
N ++F+ +A +E ++D + ++ +F++ E + G +G LL GPPGTGK+ L A
Sbjct: 636 NTGVTFDDVAGVDEAKQDFM-EVVEFLKKPERFTAVGARIPKGVLLIGPPGTGKTLLAKA 812
Query: 254 MA 255
+A
Sbjct: 813 IA 818
>TC10924 homologue to UP|Q8H9D4 (Q8H9D4) 26S proteasome AAA-ATPase subunit
RPT4a, partial (49%)
Length = 648
Score = 37.0 bits (84), Expect = 0.007
Identities = 17/36 (47%), Positives = 24/36 (66%)
Frame = +2
Query: 224 EFYRRTGKAWKRGYLLYGPPGTGKSSLIAAMANYLN 259
E + R G +G LLYGPPGTGK+ L A+A+ ++
Sbjct: 530 ELFLRVGIKPPKGVLLYGPPGTGKTLLARAIASNID 637
>TC12379 homologue to UP|O04327 (O04327) Cell division protein FtsH isolog,
partial (15%)
Length = 505
Score = 37.0 bits (84), Expect = 0.007
Identities = 21/52 (40%), Positives = 30/52 (57%)
Frame = +1
Query: 314 DNKVTLSGLLNATDGLWSCCGEEHIIVFTTNHKERLDPALLRPGRMDKQIHL 365
+ TL+ LL DG E I + +TN + LDPAL+RPGR D++I +
Sbjct: 169 ERDATLNQLLVCLDGFEG--RGEVITIASTNRPDILDPALVRPGRFDRKIFI 318
>TC14829 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulatory subunit
6B homolog, partial (28%)
Length = 662
Score = 37.0 bits (84), Expect = 0.007
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +2
Query: 339 IVFTTNHKERLDPALLRPGRMDKQI 363
++ TN + LDPALLRPGR+D++I
Sbjct: 20 VIMATNRADTLDPALLRPGRLDRKI 94
>AW719510
Length = 358
Score = 35.8 bits (81), Expect = 0.016
Identities = 13/25 (52%), Positives = 20/25 (80%)
Frame = -2
Query: 339 IVFTTNHKERLDPALLRPGRMDKQI 363
++ TN + LDPALLRPGR+D+++
Sbjct: 342 VLMATNRPDTLDPALLRPGRLDRKV 268
>AV426828
Length = 401
Score = 35.4 bits (80), Expect = 0.020
Identities = 21/54 (38%), Positives = 26/54 (47%)
Frame = +1
Query: 205 DEELQRDIVNDLDKFVRAREFYRRTGKAWKRGYLLYGPPGTGKSSLIAAMANYL 258
D RDIV+ +D+ LLYGPPGTGK+S I A+A L
Sbjct: 49 DVAAHRDIVDTIDRLTTENRLPH---------LLLYGPPGTGKTSTILAVARKL 183
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.135 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,855,428
Number of Sequences: 28460
Number of extensions: 81266
Number of successful extensions: 501
Number of sequences better than 10.0: 62
Number of HSP's better than 10.0 without gapping: 489
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 497
length of query: 473
length of database: 4,897,600
effective HSP length: 94
effective length of query: 379
effective length of database: 2,222,360
effective search space: 842274440
effective search space used: 842274440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC148917.11