Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC148718.4 + phase: 0 
         (324 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC19167                                                               157  3e-39
TC16428 similar to UP|Q9STI6 (Q9STI6) Nucleotide sugar epimerase...    32  0.19
TC8547 homologue to UP|MMK1_MEDSA (Q07176) Mitogen-activated pro...    28  2.1
TC19962                                                                27  3.6
TC16246 weakly similar to UP|Q8S2M2 (Q8S2M2) Far-red impaired re...    27  3.6
BP076640                                                               27  3.6
TC8909 similar to UP|Q9FIJ4 (Q9FIJ4) Gb|AAF26028.1, partial (69%)      27  3.6
TC8968 homologue to UP|Q41619 (Q41619) Protein kinase (Fragment)...    27  3.6
BP040015                                                               27  4.7
TC17497 UP|CAE45595 (CAE45595) S-receptor kinase-like protein 2,...    27  6.1
TC9430 similar to UP|Q96569 (Q96569) L-lactate dehydrogenase  (L...    27  6.1
AV414256                                                               26  8.0
TC14319 similar to UP|AP23_ARATH (P42736) AP2 domain transcripti...    26  8.0
TC16159 homologue to UP|Q94A23 (Q94A23) AT5g64310/MSJ1_15, parti...    26  8.0
AV769348                                                               26  8.0

>TC19167 
          Length = 512

 Score =  157 bits (396), Expect = 3e-39
 Identities = 71/87 (81%), Positives = 79/87 (90%)
 Frame = +2

Query: 238 AGEDVWKEFFQYCRESKFIHITSIDFTLLSTLAPFWVYNDMTARKWFDKGSWLLPVSLIP 297
           +G+DVWKEF+QY RESKFIHI +IDFTLLSTLAPFWVYNDMT RKWFDKGSWLLPVSLIP
Sbjct: 2   SGDDVWKEFYQYFRESKFIHIMTIDFTLLSTLAPFWVYNDMTTRKWFDKGSWLLPVSLIP 181

Query: 298 LLGPALYILLRPSLSTSAVAQSPAESE 324
            LGP LY+LLRPSLST A++Q+P E E
Sbjct: 182 FLGPGLYLLLRPSLSTMAISQTPVEPE 262


>TC16428 similar to UP|Q9STI6 (Q9STI6) Nucleotide sugar epimerase-like
           protein, partial (21%)
          Length = 551

 Score = 31.6 bits (70), Expect = 0.19
 Identities = 20/66 (30%), Positives = 30/66 (45%), Gaps = 3/66 (4%)
 Frame = +1

Query: 177 LSASFFGGIYALLPYFVLWKPPPPPVEEAEL--KTWPLNFLESKITAMILLASGIGI-II 233
           L +SFF     LL +F+L  PPPPP     +   +W     E ++       S  G+ ++
Sbjct: 166 LCSSFFLAFTLLLFFFILSPPPPPPAAPRRVLGDSWGGPLWEKRVAKSARRTSASGLTVL 345

Query: 234 YAGLAG 239
             G AG
Sbjct: 346 VTGAAG 363


>TC8547 homologue to UP|MMK1_MEDSA (Q07176) Mitogen-activated protein kinase
            homolog MMK1  (MAP kinase MSK7) (MAP kinase ERK1) ,
            partial (71%)
          Length = 1098

 Score = 28.1 bits (61), Expect = 2.1
 Identities = 12/29 (41%), Positives = 19/29 (65%)
 Frame = +1

Query: 90   DWTTSILLFLLWAALIYYVSFLSPNQTPS 118
            DW T I+ +L WA+L+ ++ +L PN   S
Sbjct: 982  DWYT-IIRYLYWASLLLHIHWLVPNSDKS 1065


>TC19962 
          Length = 374

 Score = 27.3 bits (59), Expect = 3.6
 Identities = 14/34 (41%), Positives = 17/34 (49%)
 Frame = -2

Query: 168 KSSVPVWPFLSASFFGGIYALLPYFVLWKPPPPP 201
           K+  P +P  S+SFF     L P      PPPPP
Sbjct: 235 KTQAP*FPSSSSSFFSSHSHLTPPSPANPPPPPP 134


>TC16246 weakly similar to UP|Q8S2M2 (Q8S2M2) Far-red impaired response
           protein-like, partial (4%)
          Length = 660

 Score = 27.3 bits (59), Expect = 3.6
 Identities = 11/43 (25%), Positives = 21/43 (48%)
 Frame = -1

Query: 270 APFWVYNDMTARKWFDKGSWLLPVSLIPLLGPALYILLRPSLS 312
           +PFW+ +      W DK   + P    P  GP++  ++  +L+
Sbjct: 150 SPFWILSLFGFAMWLDKSVXVKPNDSCPPFGPSIKAMIWQALT 22


>BP076640 
          Length = 542

 Score = 27.3 bits (59), Expect = 3.6
 Identities = 17/45 (37%), Positives = 23/45 (50%)
 Frame = -1

Query: 177 LSASFFGGIYALLPYFVLWKPPPPPVEEAELKTWPLNFLESKITA 221
           L  SFFG +  ++P F+ WK   P V    +      F +SKITA
Sbjct: 140 LGGSFFGFMGPIIPCFLNWKRTQPQV*FTAVN---CVFCQSKITA 15


>TC8909 similar to UP|Q9FIJ4 (Q9FIJ4) Gb|AAF26028.1, partial (69%)
          Length = 1000

 Score = 27.3 bits (59), Expect = 3.6
 Identities = 14/43 (32%), Positives = 22/43 (50%)
 Frame = -3

Query: 151 FWPLVYSMLLLPTGRSSKSSVPVWPFLSASFFGGIYALLPYFV 193
           F+P +  +LLL +    + SVP+ PF   S      + LP+ V
Sbjct: 236 FFPSLLLLLLLRSREHFRESVPIRPFTPKSLLTNPSSRLPFLV 108


>TC8968 homologue to UP|Q41619 (Q41619) Protein kinase (Fragment), partial
           (32%)
          Length = 612

 Score = 27.3 bits (59), Expect = 3.6
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = -3

Query: 242 VWKEFFQYCRESKFIHITSIDFTLLSTLAP 271
           +WK+ FQ+C  S   HITS +  + S+ AP
Sbjct: 376 IWKQHFQFCYISS-QHITSQEMGIASSHAP 290


>BP040015 
          Length = 529

 Score = 26.9 bits (58), Expect = 4.7
 Identities = 21/75 (28%), Positives = 32/75 (42%), Gaps = 4/75 (5%)
 Frame = -3

Query: 134 DGFRMNEVLVSEWYIMGF----WPLVYSMLLLPTGRSSKSSVPVWPFLSASFFGGIYALL 189
           +GF   E+LVS W+ +GF    +P        P     K  + V+P L     GG  +  
Sbjct: 485 NGFPNGEILVSSWWRLGFEEVEYPWGKPKYCCPVVHHRKDIILVFPPLREGSGGGSDSGD 306

Query: 190 PYFVLWKPPPPPVEE 204
              V+   PP  +E+
Sbjct: 305 GVSVIVALPPEDMEK 261


>TC17497 UP|CAE45595 (CAE45595) S-receptor kinase-like protein 2, complete
          Length = 2946

 Score = 26.6 bits (57), Expect = 6.1
 Identities = 16/52 (30%), Positives = 26/52 (49%)
 Frame = -2

Query: 263 FTLLSTLAPFWVYNDMTARKWFDKGSWLLPVSLIPLLGPALYILLRPSLSTS 314
           FT+  +    W  N +   K F       P +L+P+ GPAL ++  P ++TS
Sbjct: 890 FTVSYSKEISWSVNCIKNVKIFCSTCPKAPENLLPVHGPALKVIAAPFVTTS 735


>TC9430 similar to UP|Q96569 (Q96569) L-lactate dehydrogenase  (LDH) ,
           partial (49%)
          Length = 866

 Score = 26.6 bits (57), Expect = 6.1
 Identities = 16/63 (25%), Positives = 26/63 (40%)
 Frame = -2

Query: 152 WPLVYSMLLLPTGRSSKSSVPVWPFLSASFFGGIYALLPYFVLWKPPPPPVEEAELKTWP 211
           W      + +PT  +  + + VW  +       +  L   +V  KPP P VE  EL    
Sbjct: 403 WVRTV*AMAMPTLPAPMTEILVWRLVGEGGAA*VMGLKKVWVRSKPPGPRVEPEELLCMV 224

Query: 212 LNF 214
           ++F
Sbjct: 223 MSF 215


>AV414256 
          Length = 200

 Score = 26.2 bits (56), Expect = 8.0
 Identities = 13/22 (59%), Positives = 17/22 (77%)
 Frame = +2

Query: 302 ALYILLRPSLSTSAVAQSPAES 323
           A+++LL   L +SAVAQSPA S
Sbjct: 62  AVFMLLAALLVSSAVAQSPASS 127


>TC14319 similar to UP|AP23_ARATH (P42736) AP2 domain transcription factor
           RAP2.3 (Related to AP2 protein 3) (Cadmium-induced
           protein AS30), partial (37%)
          Length = 1130

 Score = 26.2 bits (56), Expect = 8.0
 Identities = 12/46 (26%), Positives = 22/46 (47%)
 Frame = -2

Query: 45  LVAMKTPFVAATTSFRRKSSSSSIVICNDSNKQNSSVEEKEEVKRD 90
           L AMK+  +A     R +   +S   C D N+ N    ++ + +R+
Sbjct: 142 LTAMKSEMIAPPHMSREREKINSTCRCYDDNESNGGKNKENKSERE 5


>TC16159 homologue to UP|Q94A23 (Q94A23) AT5g64310/MSJ1_15, partial (19%)
          Length = 605

 Score = 26.2 bits (56), Expect = 8.0
 Identities = 13/22 (59%), Positives = 17/22 (77%)
 Frame = +2

Query: 302 ALYILLRPSLSTSAVAQSPAES 323
           A+++LL   L +SAVAQSPA S
Sbjct: 62  AVFMLLAALLVSSAVAQSPASS 127


>AV769348 
          Length = 566

 Score = 26.2 bits (56), Expect = 8.0
 Identities = 15/40 (37%), Positives = 24/40 (59%)
 Frame = -3

Query: 92  TTSILLFLLWAALIYYVSFLSPNQTPSRDMYFLKKLLNLK 131
           ++S++LFLLW  L+ Y S L    +P    + LK  L+L+
Sbjct: 390 SSSVILFLLW--LM*YYSCLENLHSPDPFTFLLKVCLSLE 277


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.322    0.137    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,486,194
Number of Sequences: 28460
Number of extensions: 126641
Number of successful extensions: 981
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 968
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 978
length of query: 324
length of database: 4,897,600
effective HSP length: 90
effective length of query: 234
effective length of database: 2,336,200
effective search space: 546670800
effective search space used: 546670800
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)


Medicago: description of AC148718.4