BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148651.2 - phase: 0
(443 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12992 homologue to UP|O23065 (O23065) BAC IG005I10 (AT4G00370 ... 73 1e-13
BP077434 49 2e-06
AV777012 47 5e-06
TC17487 UP|CAE45597 (CAE45597) SAR DNA-binding protein-like prot... 30 0.80
BI419543 30 1.0
TC15902 similar to UP|Q948P0 (Q948P0) Aspartic proteinase alpha,... 30 1.0
CB826772 29 1.8
TC8593 homologue to UP|AAR83877 (AAR83877) 60S ribosomal protein... 28 2.3
BI419356 28 2.3
TC14075 homologue to UP|PRP2_MEDTR (Q40375) Repetitive proline-r... 28 2.3
TC15259 homologue to UP|AAR83877 (AAR83877) 60S ribosomal protei... 28 3.0
TC17641 homologue to GB|AAP31966.1|30387601|BT006622 At3g19640 {... 28 4.0
TC17326 homologue to UP|ACOC_CUCMC (Q42669) Aconitase (Aconitat... 28 4.0
BP063926 27 6.8
AV768607 27 6.8
TC14060 GB|AAA34124.1|170354|TOBUBI4A pentameric polyubiquitin {... 27 6.8
TC14058 GB|AAA34124.1|170354|TOBUBI4A pentameric polyubiquitin {... 27 6.8
BP028363 27 8.8
BP043389 27 8.8
TC14264 similar to UP|Q84NF9 (Q84NF9) Histone H1 (Fragment), par... 27 8.8
>TC12992 homologue to UP|O23065 (O23065) BAC IG005I10 (AT4G00370 protein),
partial (19%)
Length = 549
Score = 72.8 bits (177), Expect = 1e-13
Identities = 36/87 (41%), Positives = 53/87 (60%)
Frame = +2
Query: 357 RAGFAVNHMDVAPRYAGIVMGVSNTAGTLAGIVGVDLTGKLLEAAKASDSDLSTPESWRL 416
++G NH D+ PRYAG+++G+SNTAG LAG+ G TG +L+ SW
Sbjct: 5 QSGLYSNHQDIGPRYAGVLLGLSNTAGVLAGVFGTAATGYILQRG-----------SWND 151
Query: 417 VFFIPGLLCVFSSLVFLLFSTGERIFD 443
VF + +L + +LV+ +FSTGE+I D
Sbjct: 152 VFKVAVVLYIIGTLVWNIFSTGEKILD 232
>BP077434
Length = 375
Score = 48.9 bits (115), Expect = 2e-06
Identities = 23/41 (56%), Positives = 30/41 (73%)
Frame = -3
Query: 359 GFAVNHMDVAPRYAGIVMGVSNTAGTLAGIVGVDLTGKLLE 399
GF VN ++AP+YAG++ G+SNTAGTLA IVG G +E
Sbjct: 373 GFLVNLQEIAPQYAGVLHGMSNTAGTLAAIVGTVGAGFFVE 251
>AV777012
Length = 506
Score = 47.4 bits (111), Expect = 5e-06
Identities = 24/72 (33%), Positives = 42/72 (58%), Gaps = 1/72 (1%)
Frame = -1
Query: 369 PRYAGIVMGVSNTAGTLAGIVGVDLTGKLLEAAKASDSDLSTPESWRLVFFIPGLLCVFS 428
P YA +++G++NT G + GIVGV LTG LL++ SW + F P + +
Sbjct: 506 PEYASVLLGITNTVGAVPGIVGVALTGYLLDST----------HSWSMSLFAPSIFFYLT 357
Query: 429 -SLVFLLFSTGE 439
++V+L+F++ +
Sbjct: 356 GTVVWLVFASSK 321
>TC17487 UP|CAE45597 (CAE45597) SAR DNA-binding protein-like protein, complete
Length = 1794
Score = 30.0 bits (66), Expect = 0.80
Identities = 21/58 (36%), Positives = 27/58 (46%)
Frame = -2
Query: 136 VSLTTSGMYLGAAFGMLFLPSLVKFKGPQSVFVAESFLGFVWCFIWFRYSSDPKSSAS 193
+S +SG A F + F S S F +SFL F + F +SS P SSAS
Sbjct: 1751 ISADSSG---SACFFLFFFFSPASSPFCNSTFSVDSFLFFFFSFFTTSFSSSPSSSAS 1587
>BI419543
Length = 585
Score = 29.6 bits (65), Expect = 1.0
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +2
Query: 73 GRKILLFSFLLWSLTCALLP 92
G K L F LW+LTC+LLP
Sbjct: 140 GNKYLWLVFCLWALTCSLLP 199
>TC15902 similar to UP|Q948P0 (Q948P0) Aspartic proteinase alpha, partial
(25%)
Length = 517
Score = 29.6 bits (65), Expect = 1.0
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = +3
Query: 73 GRKILLFSFLLWSLTCALLP 92
G K L +F LW+LTC+LLP
Sbjct: 141 GSKHFLAAFCLWALTCSLLP 200
>CB826772
Length = 490
Score = 28.9 bits (63), Expect = 1.8
Identities = 21/93 (22%), Positives = 37/93 (39%)
Frame = +1
Query: 60 SQVPGGYFAQKIGGRKILLFSFLLWSLTCALLPLDPNKTLILVTARLLVGVAQGFIFPSI 119
+ + G + IG R ++ + + + L P+ LIL+ + G+ GF
Sbjct: 214 ASMAAGRISDYIGRRYTIILASIAFLSGSILTGYSPSY-LILMIGNFISGIGAGFTLIVA 390
Query: 120 HTVLAQWVPPHERSRSVSLTTSGMYLGAAFGML 152
A+ PP R SLT + +G G +
Sbjct: 391 PLYCAEISPPSHRGSLTSLTEFSINIGILLGYM 489
>TC8593 homologue to UP|AAR83877 (AAR83877) 60S ribosomal protein L19,
partial (80%)
Length = 874
Score = 28.5 bits (62), Expect = 2.3
Identities = 12/27 (44%), Positives = 19/27 (69%)
Frame = -3
Query: 259 LMNWLPTYFELGLKLSLHEMGSSKMLP 285
L N+L + EL +++SLH +GSS+ P
Sbjct: 164 LTNFLMFWRELAMEISLHSLGSSQTFP 84
>BI419356
Length = 469
Score = 28.5 bits (62), Expect = 2.3
Identities = 12/27 (44%), Positives = 19/27 (69%)
Frame = -3
Query: 259 LMNWLPTYFELGLKLSLHEMGSSKMLP 285
L N+L + EL +++SLH +GSS+ P
Sbjct: 152 LTNFLMFWRELAMEISLHSLGSSQTFP 72
>TC14075 homologue to UP|PRP2_MEDTR (Q40375) Repetitive proline-rich cell wall
protein 2 precursor, partial (98%)
Length = 1524
Score = 28.5 bits (62), Expect = 2.3
Identities = 11/33 (33%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
Frame = -1
Query: 231 IPWVKILTSLPVWAIVVNNFTFHYALY-VLMNW 262
+PWV + LP+W I+ + + LY +NW
Sbjct: 1194 VPWVTTIWWLPIWRILNRRLVYWWLLYWWFVNW 1096
>TC15259 homologue to UP|AAR83877 (AAR83877) 60S ribosomal protein L19,
partial (80%)
Length = 956
Score = 28.1 bits (61), Expect = 3.0
Identities = 12/27 (44%), Positives = 20/27 (73%)
Frame = -2
Query: 259 LMNWLPTYFELGLKLSLHEMGSSKMLP 285
L ++L + EL +++SLH +GSS+ LP
Sbjct: 163 LTSFLMFWRELAMEISLHSLGSSQTLP 83
>TC17641 homologue to GB|AAP31966.1|30387601|BT006622 At3g19640 {Arabidopsis
thaliana;}, partial (11%)
Length = 521
Score = 27.7 bits (60), Expect = 4.0
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = -3
Query: 252 FHYALYVLMNWLPTYFELGLKLSLHE 277
F ALY LMNW+P+Y +L LH+
Sbjct: 327 FCIALYFLMNWVPSY--SSKRLCLHQ 256
>TC17326 homologue to UP|ACOC_CUCMC (Q42669) Aconitase (Aconitate
hydratase) (Citrate hydro-lyase) (Fragment) , partial
(37%)
Length = 1220
Score = 27.7 bits (60), Expect = 4.0
Identities = 19/61 (31%), Positives = 28/61 (45%)
Frame = +1
Query: 355 LGRAGFAVNHMDVAPRYAGIVMGVSNTAGTLAGIVGVDLTGKLLEAAKASDSDLSTPESW 414
LG AG+ V ++ G M + L G+VG L GKL + A+D L+ +
Sbjct: 112 LGVAGWGVGGIEAEAAMLGQPMSM-----VLPGVVGFKLLGKLRDGVTATDLVLTVTQML 276
Query: 415 R 415
R
Sbjct: 277 R 279
>BP063926
Length = 487
Score = 26.9 bits (58), Expect = 6.8
Identities = 14/41 (34%), Positives = 24/41 (58%)
Frame = +3
Query: 225 KKSGVGIPWVKILTSLPVWAIVVNNFTFHYALYVLMNWLPT 265
KK G+ +K++++ +W +V FH L +L+N LPT
Sbjct: 276 KKKGL----LKLMSTPLLWNHLVRMVVFHCGLRLLLNCLPT 386
>AV768607
Length = 543
Score = 26.9 bits (58), Expect = 6.8
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = -3
Query: 413 SWRLVFFIPGLLCVFSSLVFLLFSTGERIFD 443
SW VF + L + ++V+ LFSTGERI +
Sbjct: 499 SWDDVFKVSVGLYLVGTVVWNLFSTGERILE 407
>TC14060 GB|AAA34124.1|170354|TOBUBI4A pentameric polyubiquitin {Nicotiana
sylvestris;} , partial (49%)
Length = 567
Score = 26.9 bits (58), Expect = 6.8
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +2
Query: 140 TSGMYLGAAFGMLFLPSLVKFKGPQSVFVAESFLGFVWCF 179
T ++L + G L S + +GP + + L F+WCF
Sbjct: 17 TKRVFLRISKG*FLLGSSWRMEGPSLITTSRRNLPFIWCF 136
>TC14058 GB|AAA34124.1|170354|TOBUBI4A pentameric polyubiquitin {Nicotiana
sylvestris;} , partial (43%)
Length = 563
Score = 26.9 bits (58), Expect = 6.8
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +1
Query: 140 TSGMYLGAAFGMLFLPSLVKFKGPQSVFVAESFLGFVWCF 179
T ++L + G L S + +GP + + L F+WCF
Sbjct: 397 TKRVFLRISKG*FLLGSSWRMEGPSLITTSRRNLPFIWCF 516
>BP028363
Length = 508
Score = 26.6 bits (57), Expect = 8.8
Identities = 14/44 (31%), Positives = 21/44 (46%)
Frame = +1
Query: 83 LWSLTCALLPLDPNKTLILVTARLLVGVAQGFIFPSIHTVLAQW 126
+W LTC L +P K + F+FPS+ T+L+ W
Sbjct: 238 IWCLTCVSLE-EPTKRVCFPMI--------SFLFPSLSTLLSNW 342
>BP043389
Length = 453
Score = 26.6 bits (57), Expect = 8.8
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = -1
Query: 416 LVFFIPGLLCVFSSLVFLLFSTGERI 441
+VFF L+C FS L LFS +
Sbjct: 105 VVFFFSSLICYFSKLSLFLFSYSSNV 28
>TC14264 similar to UP|Q84NF9 (Q84NF9) Histone H1 (Fragment), partial (76%)
Length = 1277
Score = 26.6 bits (57), Expect = 8.8
Identities = 30/87 (34%), Positives = 38/87 (43%), Gaps = 3/87 (3%)
Frame = -3
Query: 318 LNTIGFLVASFALIVIPSFRTSGGAVFCSSVALGF-LALGRAG--FAVNHMDVAPRYAGI 374
L+ + L A FAL +F T GG F L F ALG AG FA+ A +A
Sbjct: 858 LDALAGLAAGFALAAGLAFGT-GGFTFIRGAVLDFAFALGLAGEAFALAGAATALAFAAG 682
Query: 375 VMGVSNTAGTLAGIVGVDLTGKLLEAA 401
+ + A LA G L L+ AA
Sbjct: 681 LALAAGFALGLAAAAGFALAVVLVAAA 601
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.141 0.432
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,361,374
Number of Sequences: 28460
Number of extensions: 123170
Number of successful extensions: 968
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 963
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 967
length of query: 443
length of database: 4,897,600
effective HSP length: 93
effective length of query: 350
effective length of database: 2,250,820
effective search space: 787787000
effective search space used: 787787000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC148651.2