Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC148528.1 + phase: 0 
         (131 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BI419711                                                               28  0.67
TC8661 weakly similar to UP|O81348 (O81348) Symbiotic ammonium t...    27  0.88
TC14497                                                                26  2.0
AV426207                                                               26  2.0
BP036451                                                               25  3.3
AV766168                                                               25  4.4
AV766036                                                               25  5.7
TC14241 homologue to UP|Q9XQB4 (Q9XQB4) Photosystem I reaction c...    25  5.7
BP061495                                                               24  7.4
TC19540 weakly similar to PIR|H86283|H86283 protein T15D22.8 [im...    24  7.4
TC12618 similar to UP|MR11_ARATH (Q9XGM2) Double-strand break re...    24  7.4
NP459570 hypothetical protein [Lotus japonicus]                        24  7.4
TC18222 similar to UP|SY32_ARATH (Q9LK09) Syntaxin 32 (AtSYP32),...    24  7.4
TC9234 homologue to UP|Q7PX09 (Q7PX09) AgCP12481 (Fragment), par...    24  9.7
AV767642                                                               24  9.7
TC13975                                                                24  9.7
BP033071                                                               24  9.7
TC15916 similar to GB|AAM16242.1|20334762|AY093981 AT4g01090/F2N...    24  9.7
BP063536                                                               24  9.7

>BI419711 
          Length = 539

 Score = 27.7 bits (60), Expect = 0.67
 Identities = 11/24 (45%), Positives = 15/24 (61%)
 Frame = +1

Query: 93  HILTERERRKKMRNMFDSLHALLP 116
           HI  ER RRK+M     +L +L+P
Sbjct: 403 HIAVERNRRKQMNEYLSALRSLMP 474


>TC8661 weakly similar to UP|O81348 (O81348) Symbiotic ammonium
           transporter, partial (34%)
          Length = 1007

 Score = 27.3 bits (59), Expect = 0.88
 Identities = 12/26 (46%), Positives = 17/26 (65%)
 Frame = +1

Query: 93  HILTERERRKKMRNMFDSLHALLPEL 118
           HI+ ER RR+K+     +L AL+P L
Sbjct: 31  HIIAERRRREKLSQSLIALAALIPGL 108


>TC14497 
          Length = 874

 Score = 26.2 bits (56), Expect = 2.0
 Identities = 15/41 (36%), Positives = 22/41 (53%)
 Frame = -1

Query: 91  EMHILTERERRKKMRNMFDSLHALLPELPSKVNSFSSSISS 131
           E H   ERERR++MR    +    L     KVN+ + SI++
Sbjct: 226 EAHANVERERRERMRRKQWNERTWLVNANGKVNNTTLSINN 104


>AV426207 
          Length = 359

 Score = 26.2 bits (56), Expect = 2.0
 Identities = 16/54 (29%), Positives = 25/54 (45%)
 Frame = +3

Query: 34 ESSKKRVDTKPLNQNEGINEAEAPVSKKRNWGGVVIRSENNITTGEEKDKKYRD 87
          E + K+  TKP+  + G        S+K N+GG  +  E  +      DKK+ D
Sbjct: 36 ERNGKQELTKPVKVDRG--------SRKANFGGERLSQEVTLFKNNPSDKKFAD 173


>BP036451 
          Length = 511

 Score = 25.4 bits (54), Expect = 3.3
 Identities = 11/31 (35%), Positives = 21/31 (67%)
 Frame = +2

Query: 96  TERERRKKMRNMFDSLHALLPELPSKVNSFS 126
           TE+ERR+++   +  L +L+P  P+K++  S
Sbjct: 332 TEKERREQLNGKYKILRSLIPS-PTKIDRAS 421


>AV766168 
          Length = 369

 Score = 25.0 bits (53), Expect = 4.4
 Identities = 10/31 (32%), Positives = 15/31 (48%)
 Frame = +2

Query: 19 NHLWGDLPNSDHNLGESSKKRVDTKPLNQNE 49
          NH+WG    + HN      K + T+  NQ +
Sbjct: 5  NHMWGQFQFNTHNHLPLEGKSIQTRQNNQKK 97


>AV766036 
          Length = 518

 Score = 24.6 bits (52), Expect = 5.7
 Identities = 13/43 (30%), Positives = 23/43 (53%), Gaps = 6/43 (13%)
 Frame = +1

Query: 30  HNLGESS------KKRVDTKPLNQNEGINEAEAPVSKKRNWGG 66
           +NLG++S      K  +D KPLN + G+    +  +   ++GG
Sbjct: 61  YNLGDASTDPKPQKT*LDPKPLNHDSGLTPKNSKNTFSAHYGG 189


>TC14241 homologue to UP|Q9XQB4 (Q9XQB4) Photosystem I reaction center
           subunit III, partial (86%)
          Length = 1081

 Score = 24.6 bits (52), Expect = 5.7
 Identities = 9/22 (40%), Positives = 14/22 (62%)
 Frame = -3

Query: 103 KMRNMFDSLHALLPELPSKVNS 124
           ++ N FDSLH + PE+ +   S
Sbjct: 347 RLANCFDSLHGVRPEISATAGS 282


>BP061495 
          Length = 325

 Score = 24.3 bits (51), Expect = 7.4
 Identities = 10/31 (32%), Positives = 14/31 (44%)
 Frame = +1

Query: 19 NHLWGDLPNSDHNLGESSKKRVDTKPLNQNE 49
          NH+WG    + HN      K + T+  N  E
Sbjct: 25 NHMWGQFQFNTHNHLPLEGKSIQTRQNNPEE 117


>TC19540 weakly similar to PIR|H86283|H86283 protein T15D22.8 [imported] -
           Arabidopsis thaliana {Arabidopsis thaliana;}, partial
           (16%)
          Length = 594

 Score = 24.3 bits (51), Expect = 7.4
 Identities = 10/16 (62%), Positives = 12/16 (74%)
 Frame = +3

Query: 111 LHALLPELPSKVNSFS 126
           L  LLP+LP  VNSF+
Sbjct: 15  LECLLPDLPFSVNSFT 62


>TC12618 similar to UP|MR11_ARATH (Q9XGM2) Double-strand break repair
           protein MRE11, partial (23%)
          Length = 805

 Score = 24.3 bits (51), Expect = 7.4
 Identities = 13/37 (35%), Positives = 16/37 (43%)
 Frame = +2

Query: 16  IWENHLWGDLPNSDHNLGESSKKRVDTKPLNQNEGIN 52
           +WEN  WG    SDH           +KP +  EG N
Sbjct: 728 LWENSSWGFWCWSDH-----------SKPYSYQEGFN 805


>NP459570 hypothetical protein [Lotus japonicus]
          Length = 972

 Score = 24.3 bits (51), Expect = 7.4
 Identities = 8/20 (40%), Positives = 11/20 (55%)
 Frame = +1

Query: 10  GVQTGFIWENHLWGDLPNSD 29
           G+ +G +W N  WG   N D
Sbjct: 718 GILSGAVWANEAWGSYWNWD 777


>TC18222 similar to UP|SY32_ARATH (Q9LK09) Syntaxin 32 (AtSYP32), partial
           (37%)
          Length = 493

 Score = 24.3 bits (51), Expect = 7.4
 Identities = 9/17 (52%), Positives = 12/17 (69%)
 Frame = +2

Query: 85  YRDFDHEMHILTERERR 101
           YRD  HE H +TER ++
Sbjct: 11  YRDRTHEFHSITERLKK 61


>TC9234 homologue to UP|Q7PX09 (Q7PX09) AgCP12481 (Fragment), partial (6%)
          Length = 607

 Score = 23.9 bits (50), Expect = 9.7
 Identities = 12/32 (37%), Positives = 19/32 (58%)
 Frame = +2

Query: 60  KKRNWGGVVIRSENNITTGEEKDKKYRDFDHE 91
           K+++   V+ + +  I  GE+ D K RDFD E
Sbjct: 185 KEKSIPYVLAQIDTCIQYGEDADVKVRDFDPE 280


>AV767642 
          Length = 502

 Score = 23.9 bits (50), Expect = 9.7
 Identities = 8/19 (42%), Positives = 12/19 (63%)
 Frame = -2

Query: 73  NNITTGEEKDKKYRDFDHE 91
           N+  TGEE  + + D+ HE
Sbjct: 393 NDRRTGEESSRSHHDYQHE 337


>TC13975 
          Length = 402

 Score = 23.9 bits (50), Expect = 9.7
 Identities = 9/26 (34%), Positives = 16/26 (60%)
 Frame = -2

Query: 34  ESSKKRVDTKPLNQNEGINEAEAPVS 59
           E+ + R  T+PL  +EG+  ++ P S
Sbjct: 137 ENQRSRFQTRPLPMSEGLPPSQMPSS 60


>BP033071 
          Length = 539

 Score = 23.9 bits (50), Expect = 9.7
 Identities = 13/32 (40%), Positives = 17/32 (52%)
 Frame = +2

Query: 100 RRKKMRNMFDSLHALLPELPSKVNSFSSSISS 131
           RR+    +F  +    P LPS + SFSS  SS
Sbjct: 236 RRRHHHQIFSFILHQSPPLPSYIPSFSSPSSS 331


>TC15916 similar to GB|AAM16242.1|20334762|AY093981 AT4g01090/F2N1_13
           {Arabidopsis thaliana;}, partial (10%)
          Length = 555

 Score = 23.9 bits (50), Expect = 9.7
 Identities = 9/29 (31%), Positives = 17/29 (58%)
 Frame = -2

Query: 3   KSVDHDQGVQTGFIWENHLWGDLPNSDHN 31
           K +  DQG+   F+    ++  LP+++HN
Sbjct: 515 KLIQTDQGINYNFLHITSIFLKLPDANHN 429


>BP063536 
          Length = 504

 Score = 23.9 bits (50), Expect = 9.7
 Identities = 8/11 (72%), Positives = 11/11 (99%)
 Frame = +3

Query: 2   AKSVDHDQGVQ 12
           +K++DHDQGVQ
Sbjct: 357 SKTIDHDQGVQ 389


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.310    0.129    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,193,711
Number of Sequences: 28460
Number of extensions: 23826
Number of successful extensions: 125
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 125
length of query: 131
length of database: 4,897,600
effective HSP length: 80
effective length of query: 51
effective length of database: 2,620,800
effective search space: 133660800
effective search space used: 133660800
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 50 (23.9 bits)


Medicago: description of AC148528.1