BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148227.2 + phase: 0 /pseudo
(1075 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP033602 94 1e-19
BP053302 85 5e-17
BP054351 72 6e-13
BP054557 58 9e-09
BP058111 47 2e-05
AV766088 44 2e-04
AV406379 43 3e-04
BP063694 41 9e-04
BP064269 40 0.001
BP035283 40 0.003
AV764934 39 0.004
AV427921 39 0.006
BP078157 38 0.007
BP081717 38 0.007
TC13132 similar to UP|Q9FJA1 (Q9FJA1) Similarity to retroelement... 36 0.037
AV769206 36 0.037
BP035453 33 0.18
TC14574 31 0.91
BP080584 31 0.91
TC8316 similar to GB|AAO24559.1|27808558|BT003127 At5g39890 {Ara... 31 1.2
>BP033602
Length = 533
Score = 93.6 bits (231), Expect = 1e-19
Identities = 49/116 (42%), Positives = 65/116 (55%)
Frame = +3
Query: 33 KTIVSAKKNGGLYYLEDELETGHQLGQISSFSESFFVSNNKDDVMLWHLRLGHPSFKYLK 92
K I SAK GLYY ED G S +D +MLWH RLGHP+F+YL+
Sbjct: 156 KMIGSAKMKDGLYYFEDTFRNKAAQGLCGMSCTSV-----RDQIMLWHNRLGHPNFQYLR 320
Query: 93 IVFPKLFFGHDFSSFQCEIYEFSKQHRSSFPVQTYKPSKLFSIIHSDVWGPNRINS 148
+FP LF + SS +CE +K R+ + Q Y S+ F +IHSDVWGP++I +
Sbjct: 321 HLFPDLFKNVNCSSLECESCVLAKNQRAPYYSQPYHASRPFYLIHSDVWGPSKITT 488
>BP053302
Length = 432
Score = 85.1 bits (209), Expect = 5e-17
Identities = 47/79 (59%), Positives = 54/79 (67%), Gaps = 1/79 (1%)
Frame = -1
Query: 28 DSISGKTIVSAKKNGGLYYLEDELETGHQLGQISSFSESFFVSNNKDD-VMLWHLRLGHP 86
D SGK I AK++GGLYYL+ + ISS ESFFV NN DD VMLWHLRLGHP
Sbjct: 243 DLNSGKMIGRAKESGGLYYLDLGSASPLPPKTISSCFESFFVLNNNDDHVMLWHLRLGHP 64
Query: 87 SFKYLKIVFPKLFFGHDFS 105
SF YLK +FPKLF+ + S
Sbjct: 63 SFHYLKPLFPKLFYNKNLS 7
>BP054351
Length = 431
Score = 71.6 bits (174), Expect = 6e-13
Identities = 38/83 (45%), Positives = 46/83 (54%)
Frame = -1
Query: 28 DSISGKTIVSAKKNGGLYYLEDELETGHQLGQISSFSESFFVSNNKDDVMLWHLRLGHPS 87
D ISGK I SAK GLYY ED G S +D +MLWH RLGHP+
Sbjct: 245 DRISGKMIASAKMKDGLYYFEDTFRNKAAQGLCGMSCTSV-----RDQIMLWHNRLGHPN 81
Query: 88 FKYLKIVFPKLFFGHDFSSFQCE 110
F+YL+ +FP LF + SS +CE
Sbjct: 80 FQYLRHLFPDLFKNVNCSSLECE 12
>BP054557
Length = 538
Score = 57.8 bits (138), Expect = 9e-09
Identities = 39/100 (39%), Positives = 52/100 (52%), Gaps = 7/100 (7%)
Frame = -2
Query: 2 NLLSVSKLIWDKRCQTHFFDTHCLFQDSISGKTIVSAKKNGGLYYLEDEL-------ETG 54
NL+SVS L+ C+ FD CL Q+ + KTI +A++ GGLY + D G
Sbjct: 348 NLISVSALVKSLNCRLVIFDECCLLQERPTLKTIGAAEERGGLYAVIDPAVKAKHPPAEG 169
Query: 55 HQLGQISSFSESFFVSNNKDDVMLWHLRLGHPSFKYLKIV 94
+ Q+S F+ F D LWHLRLGH S K L I+
Sbjct: 168 NIKTQVSHFNFCQF------DCNLWHLRLGHTSSKKLAIL 67
>BP058111
Length = 570
Score = 47.0 bits (110), Expect = 2e-05
Identities = 41/129 (31%), Positives = 60/129 (45%), Gaps = 15/129 (11%)
Frame = -1
Query: 25 LFQDSISGKTIVSAKKNGGLYYL------------EDELETGHQLGQISSFSESFFVSNN 72
+ Q+ + K I +A++ G LY L ED ++T Q+S F+ F
Sbjct: 348 IHQERPTLKMIGAAEERGDLYALIDPAVKVKHPPVEDNIKT-----QVSHFNFCQF---- 196
Query: 73 KDDVMLWHLRLGHPSFKYLKIV---FPKLFFGHDFSSFQCEIYEFSKQHRSSFPVQTYKP 129
D LWHLRLGH S K L I+ FP F + C+ +KQ + SFP
Sbjct: 195 --DCNLWHLRLGHTSSKKLAILQKNFP--FITCKKITSPCDTCHMAKQKKLSFPNSVTLS 28
Query: 130 SKLFSIIHS 138
S++F +IH+
Sbjct: 27 SEIFDLIHT 1
>AV766088
Length = 501
Score = 43.5 bits (101), Expect = 2e-04
Identities = 23/66 (34%), Positives = 31/66 (46%), Gaps = 2/66 (3%)
Frame = -3
Query: 78 LWHLRLGHPSFKYLKIVFPKLFFGHDFSSFQ--CEIYEFSKQHRSSFPVQTYKPSKLFSI 135
LWH RLGHPS L+ + F ++ ++ CE F K R F F I
Sbjct: 307 LWHSRLGHPSSSALRYLRSNKFISYELLNYSPVCESCVFGKHVRLPFVSSNNVTVMPFDI 128
Query: 136 IHSDVW 141
+HSD+W
Sbjct: 127 LHSDLW 110
>AV406379
Length = 226
Score = 42.7 bits (99), Expect = 3e-04
Identities = 22/53 (41%), Positives = 30/53 (56%)
Frame = -3
Query: 78 LWHLRLGHPSFKYLKIVFPKLFFGHDFSSFQCEIYEFSKQHRSSFPVQTYKPS 130
L H RLGHPS + LK + PKL + +CE + K R+SFP T +P+
Sbjct: 167 LLHCRLGHPSLERLKQMVPKL---SHLKTLECESCQLGKHVRASFPSSTSRPA 18
>BP063694
Length = 511
Score = 41.2 bits (95), Expect = 9e-04
Identities = 43/172 (25%), Positives = 70/172 (40%), Gaps = 5/172 (2%)
Frame = -2
Query: 19 FFDTHCLFQDSISGKTIVSAKKNGGLYYLEDELETGHQLGQISSFSESFFVSNNKDDVML 78
F D + Q+ +G + + + GLY L + + L S + F L
Sbjct: 507 FLDDSFVIQNRKTGAVLGRXRCDQGLYVLNQDSQA--LLTTSSPLPRASFE--------L 358
Query: 79 WHLRLGHPSFKYLKIVFPKLFFGHDFSSFQ-----CEIYEFSKQHRSSFPVQTYKPSKLF 133
WH RLGH +F +K + D SS C + +K R F + S +
Sbjct: 357 WHSRLGHVNFDIIKQLHKHGCL--DVSSILPKPICCTSCQMAKSKRLVFHDNNKRASAVL 184
Query: 134 SIIHSDVWGPNRINSYPTKDGLSPLLMIILDFIGYIY*KKNQR*DKLSRILL 185
+IH D+ GP+ + S DG S ++ + DF + + +R S +LL
Sbjct: 183 DLIHCDLRGPSPVASI---DGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLL 37
>BP064269
Length = 506
Score = 40.4 bits (93), Expect = 0.001
Identities = 27/76 (35%), Positives = 35/76 (45%), Gaps = 12/76 (15%)
Frame = -2
Query: 27 QDSISGKTIVSAKKNGGLYYLEDE-LETGHQLGQISSFSE-----------SFFVSNNKD 74
QDS++ + I A GLYYL+ + L +SSF+ S F K
Sbjct: 301 QDSLTLRMIGKANPQSGLYYLDSRTMAKSCTLNSVSSFTSAVTSPETSSVFSTFTPCPKV 122
Query: 75 DVMLWHLRLGHPSFKY 90
LWH RLGH SF+Y
Sbjct: 121 AXCLWHYRLGHLSFQY 74
>BP035283
Length = 476
Score = 39.7 bits (91), Expect = 0.003
Identities = 28/84 (33%), Positives = 38/84 (44%), Gaps = 9/84 (10%)
Frame = -2
Query: 16 QTHFFDTHCLFQDSISGKTIVSAKKNGGLYYLEDE-LETGHQLGQISSFSE--------S 66
Q F + H Q + + + I +A GLYYL+ L+ L + F+ S
Sbjct: 331 QKMFLNPHAPNQGNHTLRMIGNANSQNGLYYLDTRTLDKMSHLNSVFPFTSVVTSIDTSS 152
Query: 67 FFVSNNKDDVMLWHLRLGHPSFKY 90
F S K LWH RLGH SF+Y
Sbjct: 151 VFPSCTKVAKCLWHYRLGHLSFQY 80
>AV764934
Length = 406
Score = 38.9 bits (89), Expect = 0.004
Identities = 33/102 (32%), Positives = 40/102 (38%), Gaps = 14/102 (13%)
Frame = -2
Query: 56 QLGQISSFSESFFVSNNKDDVML------WHLRLGHPSFKYLKIVFPKLFFGH------D 103
QLG S S S + V WH RLGHP LK V + +
Sbjct: 300 QLGYTRSMSSSPVSGRHPSSVSAFVTLSTWHSRLGHPHLDNLKRVLSSCNVSYSPKDTVE 121
Query: 104 FSSFQCEIYEFSKQHR--SSFPVQTYKPSKLFSIIHSDVWGP 143
F + C K HR S TY P F +I+SD+WGP
Sbjct: 120 FRTACC----LGKAHRLPSQMSTTTYLP---FELIYSDLWGP 16
>AV427921
Length = 284
Score = 38.5 bits (88), Expect = 0.006
Identities = 17/30 (56%), Positives = 23/30 (76%)
Frame = +1
Query: 224 QQSTCVSSPQQNGITERKNRHLLEMARALL 253
+Q T +PQQNG++ERKNR +L M R+LL
Sbjct: 1 RQ*TAAYTPQQNGVSERKNRTILNMVRSLL 90
>BP078157
Length = 395
Score = 38.1 bits (87), Expect = 0.007
Identities = 22/47 (46%), Positives = 25/47 (52%), Gaps = 2/47 (4%)
Frame = -2
Query: 78 LWHLRLGHPSFKYLKIVFPKLFFG--HDFSSFQCEIYEFSKQHRSSF 122
LWH RLGH S K LK+V + F DF S C + SK R SF
Sbjct: 148 LWHSRLGHLSDKVLKVVSNLVPFTVLPDFHSHNCNVCPLSKMRRLSF 8
>BP081717
Length = 291
Score = 38.1 bits (87), Expect = 0.007
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = -1
Query: 78 LWHLRLGHPSFKYLKIVFPKLFFGHDFSSFQCEIYEFSKQHRSSFPVQTYKPSKLF 133
+WH RLGHPS K L+ + + S+ C+ +KQ + FP+ K F
Sbjct: 168 MWHFRLGHPSSKVLQHISKVFPYVKLHSNIICDFCHMAKQTKLPFPISDTKSKACF 1
>TC13132 similar to UP|Q9FJA1 (Q9FJA1) Similarity to retroelement pol
polyprotein, partial (3%)
Length = 591
Score = 35.8 bits (81), Expect = 0.037
Identities = 28/88 (31%), Positives = 34/88 (37%), Gaps = 11/88 (12%)
Frame = +2
Query: 14 RCQTHFFDTHCLFQDSISGKTIVSAKKNGGLYYLEDELETGHQLGQISSFSE-------- 65
R Q F + QDS+ + I AK GLYY + Q SF+
Sbjct: 251 RTQLMFLNPQAPSQDSLHLRMIGKAKPKSGLYYRDTRTLDETCKLQFLSFTSAVNSTKAS 430
Query: 66 ---SFFVSNNKDDVMLWHLRLGHPSFKY 90
S F K LWH RLGH S +Y
Sbjct: 431 SVVSSFTPCTKVAECLWHYRLGHLSLQY 514
>AV769206
Length = 536
Score = 35.8 bits (81), Expect = 0.037
Identities = 17/30 (56%), Positives = 20/30 (66%)
Frame = -3
Query: 332 VFVGYSPTRKGYKCLDLNSKRFLVTMDVTF 361
VF+GYS KGYKCLD S R ++ DV F
Sbjct: 483 VFIGYSINHKGYKCLD-QSGRIYISKDVLF 397
>BP035453
Length = 550
Score = 33.5 bits (75), Expect = 0.18
Identities = 24/67 (35%), Positives = 33/67 (48%), Gaps = 6/67 (8%)
Frame = -1
Query: 28 DSISGKTIVSAKKNGGLYYLEDELETG----HQLGQISSFSESFFVSNNKDDVM--LWHL 81
DS + +TI + K GLY L T + + + S VS++ D + LWH
Sbjct: 307 DSSAWRTIGTVKAVKGLYILNKSSVTPLSHCNSVHSTVNIPSSVNVSSHFDPSVHNLWHY 128
Query: 82 RLGHPSF 88
RLGHPSF
Sbjct: 127 RLGHPSF 107
>TC14574
Length = 840
Score = 31.2 bits (69), Expect = 0.91
Identities = 20/74 (27%), Positives = 33/74 (44%), Gaps = 14/74 (18%)
Frame = -3
Query: 87 SFKYLKIVFPKLFFGHDFSSF--------------QCEIYEFSKQHRSSFPVQTYKPSKL 132
S +L++ +P LF +SF C+I S Q+ +Q ++ S L
Sbjct: 589 SLPFLQLFYPLLFSTLSTTSFLLKNQSNLIRLDDQPCQIINTSVQNEFLHTIQAHQRSAL 410
Query: 133 FSIIHSDVWGPNRI 146
FS H ++W PN +
Sbjct: 409 FS--HQNLWNPNHL 374
>BP080584
Length = 507
Score = 31.2 bits (69), Expect = 0.91
Identities = 27/92 (29%), Positives = 38/92 (40%), Gaps = 20/92 (21%)
Frame = -3
Query: 25 LFQDSISGKTIVSAKKNGGLYYL---EDELETGHQLGQISSFSESFFVSNNKDDVM---- 77
L D + K I +A+ GLY L + T +S S VS + V+
Sbjct: 328 LIMDYSAYKRIGTARAVKGLYLLMKGNPKSTTSVTCNSVSPACNSVSVSGSDSPVVDTNL 149
Query: 78 ----------LWHLRLGHPSF---KYLKIVFP 96
LWH RLGHPSF + ++ +FP
Sbjct: 148 ACNFIPSMHNLWHFRLGHPSFVKEQSIRDIFP 53
>TC8316 similar to GB|AAO24559.1|27808558|BT003127 At5g39890 {Arabidopsis
thaliana;}, partial (63%)
Length = 1796
Score = 30.8 bits (68), Expect = 1.2
Identities = 21/63 (33%), Positives = 30/63 (47%), Gaps = 7/63 (11%)
Frame = -3
Query: 386 FFEDL--ILFENMFMSHSSRPFVSKENAPDNVSEH-----TPSMSEDVTKLVATNQNSNN 438
FF DL +LF + SH+S PF+S E P + H T E TK +N +N
Sbjct: 327 FFLDLLLVLFVSADNSHNSLPFLSLEFLPIPIQTHPKTEQTDPQDEHTTKSELQQRNGSN 148
Query: 439 DSL 441
++
Sbjct: 147 SNI 139
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.355 0.158 0.554
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,692,815
Number of Sequences: 28460
Number of extensions: 286604
Number of successful extensions: 3230
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 2398
Number of HSP's successfully gapped in prelim test: 67
Number of HSP's that attempted gapping in prelim test: 772
Number of HSP's gapped (non-prelim): 2535
length of query: 1075
length of database: 4,897,600
effective HSP length: 100
effective length of query: 975
effective length of database: 2,051,600
effective search space: 2000310000
effective search space used: 2000310000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC148227.2