Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC147472.6 + phase: 0 /pseudo
         (180 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC13572                                                                30  0.32
TC10699 similar to UP|MAD2_ARATH (Q9LU93) Mitotic spindle checkp...    28  0.93
BP034914                                                               28  1.2
TC14622                                                                27  1.6
AI967750                                                               25  6.0
TC11962                                                                25  7.8
BP043696                                                               25  7.8

>TC13572 
          Length = 534

 Score = 29.6 bits (65), Expect = 0.32
 Identities = 13/26 (50%), Positives = 17/26 (65%)
 Frame = +2

Query: 101 NQSSTPITGVSTYNVTPMKLGVYFNK 126
           N  +TPIT  S +N TP +LG  FN+
Sbjct: 428 NICTTPITIFSQHNTTPRELGTVFNR 505


>TC10699 similar to UP|MAD2_ARATH (Q9LU93) Mitotic spindle checkpoint
           protein MAD2, partial (95%)
          Length = 975

 Score = 28.1 bits (61), Expect = 0.93
 Identities = 10/28 (35%), Positives = 19/28 (67%)
 Frame = -2

Query: 141 EKIDMPVFFYIDPEIEDDPKMNGINNII 168
           + + M +FF ++  I+ + K NGI+N+I
Sbjct: 860 DPLGMGIFFLLNSNIQTEAKFNGIDNLI 777


>BP034914 
          Length = 544

 Score = 27.7 bits (60), Expect = 1.2
 Identities = 11/33 (33%), Positives = 20/33 (60%)
 Frame = -2

Query: 4   MCLTGLAFGMGVLTIFASPPLYRTFCQATSYDG 36
           +CL+  +F     ++F +  L+R+FC A S+ G
Sbjct: 237 LCLSSSSFPFSYCSLFRAWSLHRSFCGAPSHGG 139


>TC14622 
          Length = 599

 Score = 27.3 bits (59), Expect = 1.6
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = +3

Query: 100 ENQSSTPITGVSTYNVTPMKLGVYF 124
           +N    P+ G +TYN++P   G YF
Sbjct: 228 DNHCPPPLFGTATYNISPTHHGNYF 302


>AI967750 
          Length = 348

 Score = 25.4 bits (54), Expect = 6.0
 Identities = 11/33 (33%), Positives = 22/33 (66%)
 Frame = +2

Query: 142 KIDMPVFFYIDPEIEDDPKMNGINNIILSYTFF 174
           + D  + FY   E +D+P +N + +I+L+Y+F+
Sbjct: 122 RTDRSLNFY---EGDDNPNVNVLRDILLTYSFY 211


>TC11962 
          Length = 354

 Score = 25.0 bits (53), Expect = 7.8
 Identities = 10/27 (37%), Positives = 17/27 (62%)
 Frame = +2

Query: 104 STPITGVSTYNVTPMKLGVYFNKIQCF 130
           STP+TGV+ +N     +G+Y   ++ F
Sbjct: 233 STPLTGVALFNPGLSSIGIYRGFMKLF 313


>BP043696 
          Length = 470

 Score = 25.0 bits (53), Expect = 7.8
 Identities = 12/42 (28%), Positives = 21/42 (49%)
 Frame = +1

Query: 32  TSYDGTVTRRERVEEKIARHDSNQTVTSREIVVQFNADISDG 73
           T+++G  T  E  EE   +H     + +RE++ +     SDG
Sbjct: 148 TAWEGVTTLAELFEEACRKHGERALLGAREVISKEAEAGSDG 273


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.322    0.138    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,909,537
Number of Sequences: 28460
Number of extensions: 33362
Number of successful extensions: 133
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 133
length of query: 180
length of database: 4,897,600
effective HSP length: 84
effective length of query: 96
effective length of database: 2,506,960
effective search space: 240668160
effective search space used: 240668160
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)


Medicago: description of AC147472.6