BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147472.2 - phase: 0
(363 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV413391 231 2e-61
AV420128 170 3e-43
AV410018 149 6e-37
TC19295 similar to UP|Q9SZ83 (Q9SZ83) AX110P-like protein, parti... 129 1e-30
TC19676 similar to UP|Q9LT42 (Q9LT42) Genomic DNA, chromosome 3,... 37 0.007
TC19899 similar to GB|AAN28894.1|23506069|AY143955 At2g28370/T1B... 35 0.015
TC14169 29 1.4
TC13937 weakly similar to UP|Q9LVE0 (Q9LVE0) Nitrate transporter... 27 4.1
AV770189 27 4.1
TC8400 similar to UP|PIRL_LYCES (Q9SEE4) Pirin-like protein, par... 27 5.4
AV776609 27 7.0
TC12751 similar to UP|LEU3_BRANA (P29102) 3-isopropylmalate dehy... 27 7.0
TC9775 26 9.2
AV774578 26 9.2
>AV413391
Length = 428
Score = 231 bits (588), Expect = 2e-61
Identities = 115/140 (82%), Positives = 125/140 (89%)
Frame = +3
Query: 10 GILGCATIARKLARAITLTPNATISAIGSRSISKAAKFAVENSLPASVRIYGSYDEVVED 69
GI+GCA IARKLARAI L PNAT+SAI SRS KA FA EN LPA+VRIYGSYDEVVED
Sbjct: 3 GIIGCANIARKLARAIALAPNATLSAIASRSKQKANTFAAENQLPATVRIYGSYDEVVED 182
Query: 70 SGVDAVYIPLPTSLHVRWAVAAANKKKHVLVEKPAALDVAELDLILEACEANGVQFMDGC 129
GVDAVYIPLPTSLHVRWAVAAA+K KHVLVEKPAALDVA+LDLILEACE+NGVQFMDG
Sbjct: 183 PGVDAVYIPLPTSLHVRWAVAAASKGKHVLVEKPAALDVAQLDLILEACESNGVQFMDGS 362
Query: 130 MWLHHPRTSNMKHLLDLSNS 149
MWLHHPRT++M+ LL LS+S
Sbjct: 363 MWLHHPRTAHMEQLLSLSHS 422
>AV420128
Length = 408
Score = 170 bits (431), Expect = 3e-43
Identities = 83/137 (60%), Positives = 100/137 (72%)
Frame = +2
Query: 69 DSGVDAVYIPLPTSLHVRWAVAAANKKKHVLVEKPAALDVAELDLILEACEANGVQFMDG 128
D VDAVY+PLPT LHV WAV AA + KHVL+EKP A++VAELD ILEACEA+GVQFMDG
Sbjct: 2 DDEVDAVYVPLPTGLHVTWAVRAAERGKHVLLEKPVAMNVAELDRILEACEAHGVQFMDG 181
Query: 129 CMWLHHPRTSNMKHLLDLSNSDGIGPVQFIHSTSTMPTTQEFLENNIRVKPELDALGALG 188
MW+HHPRT+ MK LS++ G ++ IHS EF+ ++IR KPELD LGALG
Sbjct: 182 TMWVHHPRTAKMKEA--LSDAQRFGQLKLIHSFMAYNPGPEFINHSIRAKPELDGLGALG 355
Query: 189 DLAWYCIGASLWAKGYK 205
D+ WY I A LWA Y+
Sbjct: 356 DVGWYSIRAILWAVDYE 406
>AV410018
Length = 429
Score = 149 bits (377), Expect = 6e-37
Identities = 72/122 (59%), Positives = 92/122 (75%), Gaps = 1/122 (0%)
Frame = +2
Query: 1 MTEKDT-VRFGILGCATIARKLARAITLTPNATISAIGSRSISKAAKFAVENSLPASVRI 59
M E T +RFG++GCA IARK++RAI+L PNA++ A+GSRS+ KA FA N P ++
Sbjct: 62 MAETQTKIRFGVIGCADIARKVSRAISLAPNASLYAVGSRSLDKARAFAAANGFPDGAKV 241
Query: 60 YGSYDEVVEDSGVDAVYIPLPTSLHVRWAVAAANKKKHVLVEKPAALDVAELDLILEACE 119
YGSY+ V++D VDAVY+PLPTSLHVRWA AA K KHVL+EKP AL AE D I+ ACE
Sbjct: 242 YGSYEAVLDDPEVDAVYMPLPTSLHVRWATLAARKGKHVLLEKPVALGAAEFDEIVAACE 421
Query: 120 AN 121
++
Sbjct: 422 SS 427
>TC19295 similar to UP|Q9SZ83 (Q9SZ83) AX110P-like protein, partial (35%)
Length = 593
Score = 129 bits (323), Expect = 1e-30
Identities = 67/134 (50%), Positives = 89/134 (66%)
Frame = +3
Query: 230 SLQWDQPNQTVANIHCSFHSHTSMDLSISGSNGSMHLKDFIIPYKETSASFDFTFGAKFV 289
SL W+ VA HCSF S+ +MD++ G+ G++H+ DFIIPY+E ASF + + F
Sbjct: 12 SLPWEDGK--VATFHCSFLSNLTMDITAVGTKGTLHVHDFIIPYEEKEASFYASSESSFD 185
Query: 290 DLHIGWNVRPEEVHVVNKLPQEALMVQEFAMLVASIRDSGSQPSIKWPEISRKTQLVVDA 349
DL W+ P + + LPQEALMV EFA LVA I+ + S+P KWP ISRKTQLV+DA
Sbjct: 186 DLVTRWSSPPSKHTIKTDLPQEALMVSEFARLVAEIKFNNSKPEKKWPTISRKTQLVLDA 365
Query: 350 VKKSLELGCKPVSL 363
VK S+E G +PV +
Sbjct: 366 VKASIERGLEPVQI 407
>TC19676 similar to UP|Q9LT42 (Q9LT42) Genomic DNA, chromosome 3, P1 clone:
MOE17, partial (56%)
Length = 561
Score = 36.6 bits (83), Expect = 0.007
Identities = 32/112 (28%), Positives = 53/112 (46%), Gaps = 7/112 (6%)
Frame = +3
Query: 11 ILGCATIARK--LARAITLTPNATISAIGSRSISKAAKFAVENSLPASVRIYGSY----- 63
ILG T + L R ++ + AI SR+ ++A+ AVE + + +
Sbjct: 42 ILGAGTFVKSQYLPRLSEISHLFILKAIWSRT-QESARSAVEIAHRNFAGVECKWEDDGL 218
Query: 64 DEVVEDSGVDAVYIPLPTSLHVRWAVAAANKKKHVLVEKPAALDVAELDLIL 115
D+++ D + AV + L V ++ KHVL EKPAA ++EL+ L
Sbjct: 219 DDIIHDGSITAVAVVLAGQNQVEISLRMLKAGKHVLQEKPAASCISELETAL 374
>TC19899 similar to GB|AAN28894.1|23506069|AY143955 At2g28370/T1B3.11
{Arabidopsis thaliana;}, partial (37%)
Length = 448
Score = 35.4 bits (80), Expect = 0.015
Identities = 15/35 (42%), Positives = 27/35 (76%), Gaps = 3/35 (8%)
Frame = -2
Query: 222 GVILSITTSLQWD---QPNQTVANIHCSFHSHTSM 253
GV++S T+ +W+ + N+++A+IHCS H+HTS+
Sbjct: 282 GVLVSTTSFRRWNCINRVNRSMAHIHCSSHTHTSI 178
>TC14169
Length = 964
Score = 28.9 bits (63), Expect = 1.4
Identities = 23/73 (31%), Positives = 34/73 (46%), Gaps = 2/73 (2%)
Frame = +1
Query: 61 GSYDEVVEDSGVDAVYIPLPTSLHVR--WAVAAANKKKHVLVEKPAALDVAELDLILEAC 118
GS+ VV DS V V+ L + V+ W +AA V++ +LD+I E C
Sbjct: 445 GSFAFVVYDSKVGTVFAALGSDGGVKLYWGIAADGS---VVISD-------DLDVIKEGC 594
Query: 119 EANGVQFMDGCMW 131
+ F GCM+
Sbjct: 595 AKSFAPFPTGCMF 633
>TC13937 weakly similar to UP|Q9LVE0 (Q9LVE0) Nitrate transporter
(AT3g21670/MIL23_23), partial (27%)
Length = 558
Score = 27.3 bits (59), Expect = 4.1
Identities = 17/52 (32%), Positives = 27/52 (51%)
Frame = +2
Query: 20 KLARAITLTPNATISAIGSRSISKAAKFAVENSLPASVRIYGSYDEVVEDSG 71
KL R +T+ +ATISA+G ++ A + P S + + E +E SG
Sbjct: 320 KLGRYLTIAISATISAVGMCLLTGATTIPSMKAPPCS-SVQRQHHECIEASG 472
>AV770189
Length = 517
Score = 27.3 bits (59), Expect = 4.1
Identities = 19/67 (28%), Positives = 32/67 (47%), Gaps = 5/67 (7%)
Frame = +1
Query: 10 GILGCATIARKLARAITLTPNATISAIGSRSISKAAKFAVENSLPASV-----RIYGSYD 64
G+LG KL R +T+ +ATI+A+G K + +LP+ + + +
Sbjct: 295 GLLGGFLADAKLGRYLTVVISATIAAVG------VCKLTLATTLPSMIPPPCSAVRRQHH 456
Query: 65 EVVEDSG 71
E +E SG
Sbjct: 457 ECIEASG 477
>TC8400 similar to UP|PIRL_LYCES (Q9SEE4) Pirin-like protein, partial (93%)
Length = 1352
Score = 26.9 bits (58), Expect = 5.4
Identities = 19/61 (31%), Positives = 27/61 (44%)
Frame = -1
Query: 24 AITLTPNATISAIGSRSISKAAKFAVENSLPASVRIYGSYDEVVEDSGVDAVYIPLPTSL 83
AITLT + SA+G S+ + F A I+ +V G+ IPLP +
Sbjct: 638 AITLTSTPSFSAVGMLSLRSS**FGSIILSLADKLIHSCNPLLVASPGISE*TIPLPAVI 459
Query: 84 H 84
H
Sbjct: 458 H 456
>AV776609
Length = 378
Score = 26.6 bits (57), Expect = 7.0
Identities = 17/53 (32%), Positives = 24/53 (45%)
Frame = -2
Query: 28 TPNATISAIGSRSISKAAKFAVENSLPASVRIYGSYDEVVEDSGVDAVYIPLP 80
TP AT S S+ + LPA+VR+ G+ E + V +PLP
Sbjct: 311 TPKATGSGRNSQGSGSRSTNLAPRPLPAAVRLVGTAGEGPLPTAVALPPLPLP 153
>TC12751 similar to UP|LEU3_BRANA (P29102) 3-isopropylmalate dehydrogenase,
chloroplast precursor (Beta-IPM dehydrogenase) (IMDH)
(3-IPM-DH) , partial (25%)
Length = 450
Score = 26.6 bits (57), Expect = 7.0
Identities = 20/79 (25%), Positives = 39/79 (49%), Gaps = 6/79 (7%)
Frame = +2
Query: 53 LPASVRIYGSYDEVVEDSGVDAVYIPLPTSLHVRWAVAAANKKKHVLVEKPA------AL 106
+ ASV++ + ++ S + +P PTS+ R + A +KK + + P +
Sbjct: 56 MAASVQMQLLHARPMKASPFPSFSLPRPTSMRCRCSAATPSKKSYSITLLPGDGIGPEVV 235
Query: 107 DVAELDLILEACEANGVQF 125
VA+ D++L A +G+ F
Sbjct: 236 SVAK-DVLLLAGSLHGINF 289
>TC9775
Length = 736
Score = 26.2 bits (56), Expect = 9.2
Identities = 10/23 (43%), Positives = 15/23 (64%)
Frame = +3
Query: 109 AELDLILEACEANGVQFMDGCMW 131
+ELD C +GVQ ++GC+W
Sbjct: 435 SELDKECVVCWQDGVQNLNGCIW 503
>AV774578
Length = 309
Score = 26.2 bits (56), Expect = 9.2
Identities = 14/31 (45%), Positives = 22/31 (70%), Gaps = 1/31 (3%)
Frame = -2
Query: 154 PVQFIHSTSTMPTTQEF-LENNIRVKPELDA 183
PV F+HS +P+T +F L +N+ +PELD+
Sbjct: 308 PVTFLHSLHFLPSTVDFPLLHNL--QPELDS 222
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.132 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,113,001
Number of Sequences: 28460
Number of extensions: 78322
Number of successful extensions: 407
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 403
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 406
length of query: 363
length of database: 4,897,600
effective HSP length: 91
effective length of query: 272
effective length of database: 2,307,740
effective search space: 627705280
effective search space used: 627705280
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC147472.2