BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147405.9 + phase: 0
(589 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18603 similar to UP|Q9SJF1 (Q9SJF1) T27G7.9, partial (15%) 259 7e-70
TC15912 similar to UP|NGP1_HUMAN (Q13823) Autoantigen NGP-1, par... 82 2e-16
AV406443 33 0.099
BP056197 33 0.17
CB828788 30 0.84
AV422565 30 1.1
TC10926 similar to UP|CALX_SOYBN (Q39817) Calnexin homolog precu... 30 1.4
BI418079 30 1.4
TC13804 weakly similar to UP|Q8GRK2 (Q8GRK2) Somatic embryogenes... 29 1.9
TC12062 weakly similar to UP|Q9WSV7 (Q9WSV7) DNA, complete genom... 28 3.2
BP028390 28 3.2
TC15019 UP|Q9FY16 (Q9FY16) Ferredoxin-nitrite reductase precurso... 28 3.2
AV411945 28 4.2
TC17630 similar to GB|AAL57656.1|18086395|AY065011 AT5g13210/T31... 28 4.2
TC11519 similar to UP|Q7XAF0 (Q7XAF0) Tocopherol cyclase, partia... 28 5.4
TC8203 similar to UP|Q8L9C3 (Q8L9C3) Copia-like retroelement pol... 27 7.1
BP065772 27 7.1
TC11717 similar to UP|Q7VEZ6 (Q7VEZ6) Probable GTP binding prote... 27 7.1
TC17528 similar to GB|AAP37786.1|30725528|BT008427 At1g51560 {Ar... 27 7.1
TC13235 similar to UP|Q9LQB6 (Q9LQB6) F4N2.1, partial (38%) 27 7.1
>TC18603 similar to UP|Q9SJF1 (Q9SJF1) T27G7.9, partial (15%)
Length = 714
Score = 259 bits (663), Expect = 7e-70
Identities = 131/188 (69%), Positives = 148/188 (78%)
Frame = +2
Query: 402 RHVIEEIYNISLPKPKSYESQSRPPLASELLRTYCASRGQTTSSGLPDETRASRQILKDY 461
RHVIE +Y I+LPKPKSYESQS PPLASELLR YCASRG SSGLPDET+A+RQILKDY
Sbjct: 2 RHVIEGVYKITLPKPKSYESQSLPPLASELLRVYCASRGYVASSGLPDETKAARQILKDY 181
Query: 462 IDGKLPHYEMPPGLSTQELASEDSNEHDQVNPHVSDASDIEDSSVVETELAPKLEHVLDD 521
IDGK+PHYEMPPG+S++E A ED HD + H SD S IEDS E+E AP LEHVLDD
Sbjct: 182 IDGKVPHYEMPPGVSSEEQALEDPTGHDSDDLHDSDLSGIEDSLDGESEQAPNLEHVLDD 361
Query: 522 LSSFDMANGLASNKVAPKKTKESQKHHRKPPRTKNRSWRAGNAGKDDTDGMPIARFHQKP 581
L SFD+ANG+AS KV KK+K K H+KPPR K+ SWRAGN D TDG PIARF QKP
Sbjct: 362 LKSFDLANGIASQKVT-KKSKAPHKQHKKPPRKKSHSWRAGN---DGTDGTPIARFFQKP 529
Query: 582 VNSGPLKV 589
N+GP+KV
Sbjct: 530 ANTGPVKV 553
>TC15912 similar to UP|NGP1_HUMAN (Q13823) Autoantigen NGP-1, partial (25%)
Length = 670
Score = 82.4 bits (202), Expect = 2e-16
Identities = 63/212 (29%), Positives = 100/212 (46%)
Frame = +2
Query: 175 VVDSRDPLFYRCPDLEAYAKEVDVHKNTLLLVNKADLLPASVREKWAEYFRAHDILFIFW 234
V+D+RDP RC LE + KE +K+ +LL+NK DL+PA W
Sbjct: 5 VLDARDPQGTRCYHLEKHLKENCKYKHMVLLLNKCDLIPA-------------------W 127
Query: 235 SAKAATAVLEGKKLGSSQADNMASADNPDTKIYGRDELLARLQSEAEAIVDMRRSSGSSK 294
+ K VL S + +A + + K +G+ LL+ L+ A D + S
Sbjct: 128 ATKGWLRVL------SKEYPTLAFHASIN-KSFGKGSLLSVLRQFARLKTDKQAIS---- 274
Query: 295 SSDDNASVSSSSSHVVVGFVGYPNVGKSSTINALVGQKKTGVTSTPGKTKHFQTLIISEK 354
VGF+GYPNVGKSS IN L + V PG+TK +Q + ++++
Sbjct: 275 ----------------VGFIGYPNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKR 406
Query: 355 LILCDCPGLVFPSFSSSRYEMITCGVLPIDRM 386
L DCPG+V+ + + +++ GV+ + +
Sbjct: 407 TFLIDCPGVVYQN-KDTETDVVLKGVVRVTNL 499
>AV406443
Length = 417
Score = 33.5 bits (75), Expect = 0.099
Identities = 20/71 (28%), Positives = 34/71 (47%)
Frame = +3
Query: 302 VSSSSSHVVVGFVGYPNVGKSSTINALVGQKKTGVTSTPGKTKHFQTLIISEKLILCDCP 361
++ ++ V+G +G P VGKS+ +N L G +S+PG F T + + C
Sbjct: 180 LTENTDFTVIGVIGPPGVGKSTIMNELYGFD----SSSPGMLPPFATQSEENRAMARHCS 347
Query: 362 GLVFPSFSSSR 372
+ P S+ R
Sbjct: 348 TGIEPRISAER 380
>BP056197
Length = 585
Score = 32.7 bits (73), Expect = 0.17
Identities = 15/41 (36%), Positives = 24/41 (57%)
Frame = +2
Query: 288 RSSGSSKSSDDNASVSSSSSHVVVGFVGYPNVGKSSTINAL 328
+++ S ++ + + SSH+ G VG PNVGKS+ N L
Sbjct: 77 KAAKSKEAPAERPILGRFSSHLKXGIVGLPNVGKSTLFNTL 199
>CB828788
Length = 448
Score = 30.4 bits (67), Expect = 0.84
Identities = 17/54 (31%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
Frame = +2
Query: 293 SKSSDDNASVSSSSSHVVV--GFVGYPNVGKSSTINALVGQKKTGVTSTPGKTK 344
+ +S+D +S + S + + VG PNVGKS+ +N L+ + + V G T+
Sbjct: 80 NSNSEDTSSPEAEESKLPLQLAIVGRPNVGKSTLMNTLLQEDRVLVGPEAGLTR 241
>AV422565
Length = 470
Score = 30.0 bits (66), Expect = 1.1
Identities = 17/56 (30%), Positives = 35/56 (62%), Gaps = 5/56 (8%)
Frame = +3
Query: 314 VGYPNVGKSSTINALVGQKKTGVTSTPGKTKHFQTLIISEK-----LILCDCPGLV 364
+G VGKSST+N+++G++ V+ +P +++ + +++S L + D PGL+
Sbjct: 87 MGKGGVGKSSTVNSIIGERV--VSISPFQSEGPRPVMVSRSRAGFTLNIIDTPGLI 248
>TC10926 similar to UP|CALX_SOYBN (Q39817) Calnexin homolog precursor,
partial (31%)
Length = 629
Score = 29.6 bits (65), Expect = 1.4
Identities = 11/37 (29%), Positives = 19/37 (50%)
Frame = -2
Query: 538 PKKTKESQKHHRKPPRTKNRSWRAGNAGKDDTDGMPI 574
P +T + HHRK T + W++ A + T+G +
Sbjct: 154 PHRTIRRRSHHRKRRETATKRWKSRGAPRRSTEGFAL 44
>BI418079
Length = 622
Score = 29.6 bits (65), Expect = 1.4
Identities = 23/70 (32%), Positives = 29/70 (40%), Gaps = 2/70 (2%)
Frame = +1
Query: 417 KSYESQSRPPLASELLRTYCASRGQTTSSGLPDETRASRQI--LKDYIDGKLPHYEMPPG 474
KS + P + + L T R T SG SR I LKD I G H E PP
Sbjct: 355 KSEPTDVHVPKSRKHLATILTKRAGTGRSG------CSRSIANLKDVIHGSKRHLEKPPS 516
Query: 475 LSTQELASED 484
S + + S +
Sbjct: 517 CSPRSIGSSE 546
>TC13804 weakly similar to UP|Q8GRK2 (Q8GRK2) Somatic embryogenesis receptor
kinase 1, partial (7%)
Length = 602
Score = 29.3 bits (64), Expect = 1.9
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +2
Query: 359 DCPGLVFPSFSSSRYEMITCGVLPID 384
D P L+FP FSSS ++ G +PID
Sbjct: 374 DFPSLLFPFFSSS*NYLVCSGYMPID 451
>TC12062 weakly similar to UP|Q9WSV7 (Q9WSV7) DNA, complete genome,
isolate:TTV SANBAN, partial (4%)
Length = 578
Score = 28.5 bits (62), Expect = 3.2
Identities = 11/34 (32%), Positives = 16/34 (46%)
Frame = -3
Query: 527 MANGLASNKVAPKKTKESQKHHRKPPRTKNRSWR 560
+A L P+ +Q+HHR R +R WR
Sbjct: 483 LARRLKRGATPPRSNTAAQQHHRNKSRRSHRRWR 382
>BP028390
Length = 288
Score = 28.5 bits (62), Expect = 3.2
Identities = 19/60 (31%), Positives = 30/60 (49%), Gaps = 3/60 (5%)
Frame = +3
Query: 440 GQTTSSGLPDETRA---SRQILKDYIDGKLPHYEMPPGLSTQELASEDSNEHDQVNPHVS 496
G ++ SG ++TR ++ K + GKL H +PP L Q L +++ NP VS
Sbjct: 72 GHSSYSG-GEKTRTLNCNKNATKHHTXGKLXHSTLPPRLYNQCLLVATXKNNEERNPXVS 248
>TC15019 UP|Q9FY16 (Q9FY16) Ferredoxin-nitrite reductase precursor , complete
Length = 2098
Score = 28.5 bits (62), Expect = 3.2
Identities = 18/46 (39%), Positives = 30/46 (65%), Gaps = 1/46 (2%)
Frame = -1
Query: 295 SSDDNASVSSSS-SHVVVGFVGYPNVGKSSTINALVGQKKTGVTST 339
+SD N S+ SSS +H+++ F +P V +S I+ + Q TG+TS+
Sbjct: 1042 TSDLNTSIPSSSINHIILVFCLFPLVPRSLYISRIALQ--TGITSS 911
>AV411945
Length = 405
Score = 28.1 bits (61), Expect = 4.2
Identities = 29/136 (21%), Positives = 62/136 (45%), Gaps = 2/136 (1%)
Frame = +3
Query: 15 KQHNQMIQQTKEKGKIYKKKFLESFTEVSDIDAIIEQADEDVDEQQLLDIPLPPPTALIN 74
K N + Q +++ K+ ++ + E+ + A +++ +E + E +
Sbjct: 48 KLANFLKQASEDAKKLVDEERAFARAEIDNARAAVQRVEEALKEHE-------------R 188
Query: 75 LDHASGSDFNGLTVEEMKKEQKIEEALHASSLRVPRRPF-WSAEMSADELH-ANETQHFL 132
+ ASG +E++KKE ++EA L P + E+ A A +T+H+L
Sbjct: 189 MSQASGKQ----DLEQLKKE--VQEARRIKMLHQPSKVMDMEHELQALRAQLAEKTRHYL 350
Query: 133 TWRRSLARLEENKKLV 148
++ LAR ++ ++ V
Sbjct: 351 RLQKELARTKKGEENV 398
>TC17630 similar to GB|AAL57656.1|18086395|AY065011 AT5g13210/T31B5_30
{Arabidopsis thaliana;}, partial (27%)
Length = 625
Score = 28.1 bits (61), Expect = 4.2
Identities = 26/93 (27%), Positives = 45/93 (47%), Gaps = 8/93 (8%)
Frame = -3
Query: 289 SSGSSKSSDDNASVSS--------SSSHVVVGFVGYPNVGKSSTINALVGQKKTGVTSTP 340
S+ +S +++ NA+ +S +SH + F +P + KSSTI + TS P
Sbjct: 347 STLNSDTNNPNATDTSMGIPLILPDTSHTAIQFFIFPFLSKSSTILFHC----SSATSPP 180
Query: 341 GKTKHFQTLIISEKLILCDCPGLVFPSFSSSRY 373
++ + + K +VFP+F+SSRY
Sbjct: 179 SQSLNDPIIS*GSK---APAAIVVFPAFTSSRY 90
>TC11519 similar to UP|Q7XAF0 (Q7XAF0) Tocopherol cyclase, partial (19%)
Length = 598
Score = 27.7 bits (60), Expect = 5.4
Identities = 12/53 (22%), Positives = 26/53 (48%)
Frame = -3
Query: 373 YEMITCGVLPIDRMTQHRECVQVVANRVPRHVIEEIYNISLPKPKSYESQSRP 425
++++ G P R E ++ +P ++ I+N+ +PK S ++ RP
Sbjct: 197 FQVLNHGPPPTSRAAMSLETSNMIFPLLPSYLRSHIFNVRIPKHVSLQACERP 39
>TC8203 similar to UP|Q8L9C3 (Q8L9C3) Copia-like retroelement pol
polyprotein, partial (96%)
Length = 1198
Score = 27.3 bits (59), Expect = 7.1
Identities = 17/75 (22%), Positives = 33/75 (43%)
Frame = +2
Query: 225 RAHDILFIFWSAKAATAVLEGKKLGSSQADNMASADNPDTKIYGRDELLARLQSEAEAIV 284
R HD + + AKA T ++ + G+S M A N D DE+ + ++ +
Sbjct: 467 RIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQE 646
Query: 285 DMRRSSGSSKSSDDN 299
+ G++ D++
Sbjct: 647 ALSAPIGAAADFDED 691
>BP065772
Length = 495
Score = 27.3 bits (59), Expect = 7.1
Identities = 24/92 (26%), Positives = 40/92 (43%), Gaps = 15/92 (16%)
Frame = +1
Query: 264 TKIYGRDELLARLQSEAEAIVDMRRSSGSSKSS----------DDNASVSSSSSHVVVGF 313
TK + + L L EAI ++ + S S + + +D+ S+ HVV
Sbjct: 55 TKTWNPNLLAGNLTDPMEAIEELVQLSDSMRQATAVLADDEDIEDSKRRPSTFLHVVA-- 228
Query: 314 VGYPNVGKSSTINALVGQK-----KTGVTSTP 340
+G GKS+ +N+L+G + G T P
Sbjct: 229 LGNVGAGKSAALNSLIGHPVLPTGENGATRAP 324
>TC11717 similar to UP|Q7VEZ6 (Q7VEZ6) Probable GTP binding protein, partial
(5%)
Length = 629
Score = 27.3 bits (59), Expect = 7.1
Identities = 13/32 (40%), Positives = 19/32 (58%)
Frame = +3
Query: 298 DNASVSSSSSHVVVGFVGYPNVGKSSTINALV 329
+ ++ S S +G +G PNVGKSS I L+
Sbjct: 24 EERTLPSRSRS*SLGLIGLPNVGKSSLIIVLI 119
>TC17528 similar to GB|AAP37786.1|30725528|BT008427 At1g51560 {Arabidopsis
thaliana;}, partial (20%)
Length = 442
Score = 27.3 bits (59), Expect = 7.1
Identities = 15/53 (28%), Positives = 26/53 (48%)
Frame = -2
Query: 472 PPGLSTQELASEDSNEHDQVNPHVSDASDIEDSSVVETELAPKLEHVLDDLSS 524
P S +E+ ++ S+ H + H A D+E T+ P+ HV+ D +S
Sbjct: 252 PCNNSHEEIWNK*SHNHHEALNH*DGAIDVESEKDKVTQSWPEFHHVISDGAS 94
>TC13235 similar to UP|Q9LQB6 (Q9LQB6) F4N2.1, partial (38%)
Length = 467
Score = 27.3 bits (59), Expect = 7.1
Identities = 14/39 (35%), Positives = 24/39 (60%)
Frame = +3
Query: 411 ISLPKPKSYESQSRPPLASELLRTYCASRGQTTSSGLPD 449
IS P+P + + S+PP +++ +R +SR T S LP+
Sbjct: 201 ISPPRPPNMPTSSKPPPSAKRIR---SSRSPTPSPSLPN 308
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.130 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,886,567
Number of Sequences: 28460
Number of extensions: 134081
Number of successful extensions: 636
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 627
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 632
length of query: 589
length of database: 4,897,600
effective HSP length: 95
effective length of query: 494
effective length of database: 2,193,900
effective search space: 1083786600
effective search space used: 1083786600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC147405.9