BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146745.5 + phase: 0
(1063 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8961 similar to GB|BAB71852.1|16902294|AB062739 kinesin-relate... 204 8e-53
BP062438 112 5e-29
TC8673 similar to UP|Q9LHL9 (Q9LHL9) Kinesin (Centromere protein... 120 1e-27
AV777715 82 4e-16
BP043656 78 6e-15
BP040614 73 2e-13
CB829109 65 7e-11
TC15461 similar to UP|O24147 (O24147) Kinesin-like protein, part... 63 3e-10
AV407795 59 3e-09
TC15187 similar to UP|O24147 (O24147) Kinesin-like protein, part... 55 4e-08
AV779203 54 1e-07
TC13326 52 5e-07
TC8966 similar to UP|Q9M5Q3 (Q9M5Q3) S-ribonuclease binding prot... 52 6e-07
TC16011 similar to UP|Q8LAY3 (Q8LAY3) Inhibitor of apoptosis-lik... 51 1e-06
BP063922 48 7e-06
BP030839 48 9e-06
TC15750 similar to UP|Q8LAY3 (Q8LAY3) Inhibitor of apoptosis-lik... 47 2e-05
BP077155 47 2e-05
BP059128 45 5e-05
AV766525 45 6e-05
>TC8961 similar to GB|BAB71852.1|16902294|AB062739 kinesin-related protein
{Arabidopsis thaliana;} , partial (8%)
Length = 735
Score = 204 bits (518), Expect = 8e-53
Identities = 98/117 (83%), Positives = 105/117 (88%)
Frame = +1
Query: 947 VAESNIDKISGDGEAHTNDPKTNDSESNIISKEQTLDVSEPNNETPKEEPLVVRLKARMQ 1006
V ESNI+K + DGEA T+D KTND E++ I KEQ LD SEP NE PKEEPLVVRLKARMQ
Sbjct: 16 VTESNINKENVDGEAPTDDTKTNDIENDTIPKEQILDGSEPGNELPKEEPLVVRLKARMQ 195
Query: 1007 DMKEKELKHLGNGDANSHVCKVCFESSTAAILLPCRHFCLCKSCSLACSECPICRTN 1063
+MKEKELKHLGNGDANSHVCKVCFESSTAAILLPCRHFCLCKSCSLACSECPICRT+
Sbjct: 196 EMKEKELKHLGNGDANSHVCKVCFESSTAAILLPCRHFCLCKSCSLACSECPICRTS 366
>BP062438
Length = 498
Score = 112 bits (279), Expect(2) = 5e-29
Identities = 54/92 (58%), Positives = 69/92 (74%), Gaps = 1/92 (1%)
Frame = +3
Query: 160 GVNGTVFAYGVTSSGKTHTMHGDQNSPGIIPLAIKDVFSSIQDTPGREFLLRVSYLEIYN 219
G NGTVFAYG T+SGKTHTM G + PG+IP A++D+F IQ REFLLR+SY+EIYN
Sbjct: 222 GFNGTVFAYGQTNSGKTHTMRGSKADPGVIPRAVRDLFQIIQQDVDREFLLRMSYMEIYN 401
Query: 220 EVINDLLDPTGQNLRVREDAQ-GTYVEGIKEE 250
E INDLL P + L++ E+ + G YV G++EE
Sbjct: 402 EEINDLLAPEHRKLQIHENLERGIYVAGLREE 497
Score = 33.9 bits (76), Expect(2) = 5e-29
Identities = 21/70 (30%), Positives = 34/70 (48%)
Frame = +2
Query: 88 DSISVTIRFRPLSEREYHRGDEIAWYADGDKIVRNEFNPATAYAFDKVFGPHTNSDEVYE 147
+ I VT+R RPLS W G+ I N ++ + FD++F + + +VY
Sbjct: 20 ERIHVTVRARPLSPGGAKTS---LWRISGNSIYLP--NHSSKFEFDQIFSDNCETSQVYH 184
Query: 148 VAAKPVVKAA 157
K +V+AA
Sbjct: 185 ARTKHIVEAA 214
>TC8673 similar to UP|Q9LHL9 (Q9LHL9) Kinesin (Centromere protein) like
heavy chain-like protein, partial (11%)
Length = 819
Score = 120 bits (300), Expect = 1e-27
Identities = 78/173 (45%), Positives = 105/173 (60%)
Frame = +2
Query: 780 VQNQKLSEEASYAKELASAAAVELKNLAGEVTKLSLQNAKLEKELMAARNLANTRSAVTH 839
++N+KL+EE++YAK LASAAAVELK L+ EV KL N KL EL A++N + T+ +
Sbjct: 5 IRNRKLAEESTYAKGLASAAAVELKALSEEVAKLMNHNEKLAAELAASKN-SPTQRRTSG 181
Query: 840 TVNGVHRKYNDPRSGRKARVSSRANENVGPGRDELESWSLEVDDLKMELQARKQREAALE 899
T+ P R++ V R N+ G D K EL K+RE + E
Sbjct: 182 TI---------PNGRRESHVKPRRNDQGGSN-----------SDTKRELALSKEREQSYE 301
Query: 900 AALAEKEIMEEEHRNRVEEAKKRESSLENDLANMWVLVAKLKKEVGVVAESNI 952
AAL EK E E + +VEE+K+RE+ LEN+LANMWVLVAKLKK G AE+++
Sbjct: 302 AALLEKNQKEAELQRKVEESKQREAYLENELANMWVLVAKLKKSHG--AETDV 454
>AV777715
Length = 398
Score = 82.0 bits (201), Expect = 4e-16
Identities = 47/70 (67%), Positives = 53/70 (75%), Gaps = 5/70 (7%)
Frame = +2
Query: 2 ASSSRAPSNSPFSHRKPSTPY-----SSASSFTAGKLMPRSCSTSASSFFNSGGGADRSM 56
+SSSRA S+SPFSHRKPSTP SS SSFT GKLMPRSCS+SASSFFNS RS
Sbjct: 188 SSSSRARSSSPFSHRKPSTPSYSPASSSYSSFTNGKLMPRSCSSSASSFFNSADCGGRS- 364
Query: 57 LTPSRSQSES 66
+ PSRS+S +
Sbjct: 365 IAPSRSESST 394
>BP043656
Length = 412
Score = 78.2 bits (191), Expect = 6e-15
Identities = 34/37 (91%), Positives = 34/37 (91%)
Frame = -2
Query: 1027 KVCFESSTAAILLPCRHFCLCKSCSLACSECPICRTN 1063
KVCFES TAAILLP RHFCLCKSCSLACSECPICR N
Sbjct: 411 KVCFESPTAAILLPSRHFCLCKSCSLACSECPICRPN 301
>BP040614
Length = 504
Score = 73.2 bits (178), Expect = 2e-13
Identities = 57/165 (34%), Positives = 86/165 (51%), Gaps = 8/165 (4%)
Frame = +1
Query: 193 IKDVFSSIQDTPGR-EFLLRVSYLEIYNEVINDLLDPTGQNLR--VREDAQ--GTYVEGI 247
++D+F +D ++ + V +EIYNE + DLL G N R +R ++Q G V
Sbjct: 22 LRDLFHISKDRADSIKYEVFVQMIEIYNEQVRDLLVSNGSNRRLDIRNNSQLNGLNVPDA 201
Query: 248 KEEVVLSPGHALSFIAAGEEHRHVGSNNFNLFSSRSHTIFTLMIESSAHGEEY--DGVIF 305
V L + G+++R VG+ N SSRSH++ T+ + G E ++
Sbjct: 202 YLVPVTCTQDVLYLMEVGQKNRAVGATALNERSSRSHSVLTVHV----RGRELVSGSILR 369
Query: 306 SQLNLIDLAGSES-SKTETTGLRRKEGSYINKSLLTLGTVIGKLS 349
L+L+DLAGSE K+E G R KE +IN+SL LG VI L+
Sbjct: 370 GCLHLVDLAGSERVEKSEAVGERLKEAQHINRSLSALGDVISALA 504
>CB829109
Length = 514
Score = 64.7 bits (156), Expect = 7e-11
Identities = 51/153 (33%), Positives = 72/153 (46%), Gaps = 4/153 (2%)
Frame = +2
Query: 151 KPVVKAAMEGVNGTVFAYGVTSSGKTHTMHGDQNSPGIIPL-AIKDVFSSIQDT--PGRE 207
+P++ E T FAYG T SGKT+TM +PL A +D+ + ++
Sbjct: 14 EPIIPTIFERTKATCFAYGQTGSGKTYTMQP-------LPLRAAEDLVRQLHRPVYQNQK 172
Query: 208 FLLRVSYLEIYNEVINDLLDPTGQNLRVREDA-QGTYVEGIKEEVVLSPGHALSFIAAGE 266
F L +SY EIY + DLL + L +RED Q + G++E V FI G
Sbjct: 173 FKLWLSYFEIYGGKLFDLLSDR-KKLCMREDGRQQVCIVGLQEFEVFDVQIVKEFIEKGN 349
Query: 267 EHRHVGSNNFNLFSSRSHTIFTLMIESSAHGEE 299
R GS N SSRSH I L+++ +E
Sbjct: 350 AARSTGSTGANEESSRSHAILQLVVKKHPEVKE 448
>TC15461 similar to UP|O24147 (O24147) Kinesin-like protein, partial (9%)
Length = 527
Score = 62.8 bits (151), Expect = 3e-10
Identities = 33/83 (39%), Positives = 48/83 (57%)
Frame = +3
Query: 342 GTVIGKLSEGKSSHVPYRDSKLTRLLQSSLSGHGHVSLICTVTPASSNMEETHNTLKFAS 401
G VI LS G H+PYR+ KLT L+ SL G+ + V+P S+++ETHN+L +AS
Sbjct: 3 GDVISALSSG-GQHIPYRNHKLTMLMSDSLGGNAKTLMFVNVSPIESSLDETHNSLMYAS 179
Query: 402 RAKRVEIYASRNKIIDEKSLIKK 424
R + + S+N E +KK
Sbjct: 180 RVRSIVNDPSKNVSSKEIMRLKK 248
>AV407795
Length = 424
Score = 59.3 bits (142), Expect = 3e-09
Identities = 51/139 (36%), Positives = 66/139 (46%), Gaps = 4/139 (2%)
Frame = +2
Query: 214 YLEIYNEVINDLLDPTGQNLRVREDA-QGTYVEGIKEEVVLSPGHALSFIAAGEEHRHVG 272
+ EIY + DLL+ + L +RED Q + G++E V I G R G
Sbjct: 2 FFEIYGGKLFDLLNDR-KKLFIREDGKQQVCIVGLQEYCVSDVESIKELIERGNATRSTG 178
Query: 273 SNNFNLFSSRSHTIFTLMIESSAHGEEYDGV-IFSQLNLIDLAGSE--SSKTETTGLRRK 329
+ N SSRSH I L I+ S G E +L+ IDLAGSE + T+ R
Sbjct: 179 TTGANEESSRSHAILQLAIKRSVDGNESKPPRPVGKLSFIDLAGSERGADTTDNDKQTRI 358
Query: 330 EGSYINKSLLTLGTVIGKL 348
EG+ INKSLL L I L
Sbjct: 359 EGAEINKSLLALKECIRAL 415
>TC15187 similar to UP|O24147 (O24147) Kinesin-like protein, partial (8%)
Length = 611
Score = 55.5 bits (132), Expect = 4e-08
Identities = 26/69 (37%), Positives = 42/69 (60%)
Frame = +2
Query: 356 VPYRDSKLTRLLQSSLSGHGHVSLICTVTPASSNMEETHNTLKFASRAKRVEIYASRNKI 415
+PYR+ KLT L+ SL G+ + V+P S+++ETHN+L +ASR + + S+N
Sbjct: 2 IPYRNHKLTMLMSDSLGGNAQTLMFVNVSPVESSLDETHNSLMYASRVRSIINDPSQNVA 181
Query: 416 IDEKSLIKK 424
E + +KK
Sbjct: 182 SKEIARLKK 208
>AV779203
Length = 487
Score = 53.9 bits (128), Expect = 1e-07
Identities = 19/37 (51%), Positives = 26/37 (69%)
Frame = -1
Query: 1025 VCKVCFESSTAAILLPCRHFCLCKSCSLACSECPICR 1061
+C++CFE + +LLPCRH LC +CS C +CPI R
Sbjct: 382 LCRICFEGEISVVLLPCRHRVLCSTCSEKCKKCPISR 272
>TC13326
Length = 578
Score = 52.0 bits (123), Expect = 5e-07
Identities = 40/162 (24%), Positives = 63/162 (38%)
Frame = +2
Query: 900 AALAEKEIMEEEHRNRVEEAKKRESSLENDLANMWVLVAKLKKEVGVVAESNIDKISGDG 959
AA + E+ E R E R + EN+ +V L + V E N ++ D
Sbjct: 2 AAKKKTELEEFLIRLEAENQLWRRVAQENE-----AVVLSLHNTLEQVKERNRGLVAEDA 166
Query: 960 EAHTNDPKTNDSESNIISKEQTLDVSEPNNETPKEEPLVVRLKARMQDMKEKELKHLGNG 1019
E++ D ++ N + +E T + E ET ++ +V
Sbjct: 167 ESYCCD----ENRGNRVMQEGTGETEEIGKETTEQITMV--------------------- 271
Query: 1020 DANSHVCKVCFESSTAAILLPCRHFCLCKSCSLACSECPICR 1061
CK C S+ + LPCRH C CK+C CP+C+
Sbjct: 272 ------CKCCHSRSSCFMFLPCRHLCSCKACEPFLQACPVCK 379
>TC8966 similar to UP|Q9M5Q3 (Q9M5Q3) S-ribonuclease binding protein SBP1,
partial (48%)
Length = 611
Score = 51.6 bits (122), Expect = 6e-07
Identities = 20/48 (41%), Positives = 28/48 (57%), Gaps = 1/48 (2%)
Frame = +2
Query: 1017 GNGDANSHV-CKVCFESSTAAILLPCRHFCLCKSCSLACSECPICRTN 1063
GN D + CK C + +LLPC+H CLCK C S CP+C+++
Sbjct: 278 GNSDMKEMMTCKACRVNEVTMVLLPCKHLCLCKDCESKLSFCPLCQSS 421
>TC16011 similar to UP|Q8LAY3 (Q8LAY3) Inhibitor of apoptosis-like protein,
partial (16%)
Length = 487
Score = 50.8 bits (120), Expect = 1e-06
Identities = 17/43 (39%), Positives = 26/43 (59%)
Frame = +2
Query: 1020 DANSHVCKVCFESSTAAILLPCRHFCLCKSCSLACSECPICRT 1062
D+ S +C+ C + + ++LPCRH CLC C CPIC++
Sbjct: 137 DSGSRLCRNCGKDESCVLILPCRHLCLCTVCGSTLHTCPICKS 265
>BP063922
Length = 537
Score = 48.1 bits (113), Expect = 7e-06
Identities = 30/67 (44%), Positives = 43/67 (63%), Gaps = 1/67 (1%)
Frame = +2
Query: 305 FSQLNLIDLAGSESSKTE-TTGLRRKEGSYINKSLLTLGTVIGKLSEGKSSHVPYRDSKL 363
+S+L+L+DLAGSE S TE +G R + ++ KSL LG V+ L+ K VPY +S L
Sbjct: 338 YSKLSLVDLAGSEGSITEDDSGERVTDLLHVMKSLSALGDVLSSLT-SKKDIVPYENSAL 514
Query: 364 TRLLQSS 370
T++L S
Sbjct: 515 TKVLADS 535
>BP030839
Length = 379
Score = 47.8 bits (112), Expect = 9e-06
Identities = 15/43 (34%), Positives = 24/43 (54%)
Frame = -3
Query: 1020 DANSHVCKVCFESSTAAILLPCRHFCLCKSCSLACSECPICRT 1062
D +C+ C + + ++LPCRH C+C C CPIC++
Sbjct: 179 DRRRRLCRKCGKEESCVLILPCRHLCVCTLCGSTLHHCPICKS 51
>TC15750 similar to UP|Q8LAY3 (Q8LAY3) Inhibitor of apoptosis-like protein,
partial (14%)
Length = 547
Score = 46.6 bits (109), Expect = 2e-05
Identities = 15/36 (41%), Positives = 21/36 (57%)
Frame = +3
Query: 1025 VCKVCFESSTAAILLPCRHFCLCKSCSLACSECPIC 1060
+C+ C + +LLPCRH CLC +C CP+C
Sbjct: 75 MCRSCGVKESVVLLLPCRHLCLCTACGSTVRNCPVC 182
>BP077155
Length = 432
Score = 46.6 bits (109), Expect = 2e-05
Identities = 15/37 (40%), Positives = 22/37 (58%)
Frame = -2
Query: 1026 CKVCFESSTAAILLPCRHFCLCKSCSLACSECPICRT 1062
CK C++ + ++LPCRH C+C C CP+C T
Sbjct: 275 CKGCWKRVASVVVLPCRHLCVCTECDAQFRACPVCLT 165
>BP059128
Length = 362
Score = 45.4 bits (106), Expect = 5e-05
Identities = 16/36 (44%), Positives = 19/36 (52%)
Frame = -3
Query: 1025 VCKVCFESSTAAILLPCRHFCLCKSCSLACSECPIC 1060
VCK C + + LPCRH CKSC CP+C
Sbjct: 339 VCKSCHSRKSCFLFLPCRHLASCKSCDAILKACPVC 232
>AV766525
Length = 564
Score = 45.1 bits (105), Expect = 6e-05
Identities = 14/37 (37%), Positives = 21/37 (55%)
Frame = -1
Query: 1025 VCKVCFESSTAAILLPCRHFCLCKSCSLACSECPICR 1061
+C +C E ++ +PC H C C CS + CP+CR
Sbjct: 417 LCVICLEKEYNSVFVPCGHMCCCTGCSSHLTSCPLCR 307
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.309 0.126 0.339
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,668,098
Number of Sequences: 28460
Number of extensions: 184797
Number of successful extensions: 1115
Number of sequences better than 10.0: 88
Number of HSP's better than 10.0 without gapping: 1092
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1103
length of query: 1063
length of database: 4,897,600
effective HSP length: 100
effective length of query: 963
effective length of database: 2,051,600
effective search space: 1975690800
effective search space used: 1975690800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146745.5