BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146330.2 + phase: 0
(223 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10448 similar to GB|CAA47753.1|600387|ATPTSUB proteosome subun... 233 2e-62
TC17393 homologue to UP|PSB1_PETHY (O82531) Proteasome subunit b... 141 1e-34
TC11663 homologue to UP|Q8VZC4 (Q8VZC4) Proteasome subunit, comp... 82 6e-17
TC14283 homologue to UP|O23716 (O23716) Multicatalytic endopepti... 72 6e-14
BG662283 72 8e-14
TC11385 similar to UP|Q944Q7 (Q944Q7) At1g54990/F14C21_5, partia... 28 1.3
BP069765 28 1.7
TC15352 similar to GB|AAM70520.1|21700793|AY124811 At2g22250/T26... 27 2.8
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 27 3.7
BI419694 26 4.8
AV779816 26 6.3
TC15083 similar to UP|GAR1_YEAST (P28007) snoRNP protein GAR1, p... 26 6.3
TC7894 weakly similar to UP|Q93YY0 (Q93YY0) 68 kDa protein HP68,... 26 6.3
>TC10448 similar to GB|CAA47753.1|600387|ATPTSUB proteosome subunit
{Arabidopsis thaliana;} , partial (53%)
Length = 452
Score = 233 bits (594), Expect = 2e-62
Identities = 112/117 (95%), Positives = 114/117 (96%)
Frame = +3
Query: 1 MTKQHANWSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMAS 60
MTKQHANWSPYDNNGGSCVAIAG+DYCVIAADTRMSTGYNILTR+YSKIS LAEK VMAS
Sbjct: 102 MTKQHANWSPYDNNGGSCVAIAGADYCVIAADTRMSTGYNILTREYSKISQLAEKSVMAS 281
Query: 61 SGFQADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLG 117
SGFQADVKALQKVLSARHL YQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLG
Sbjct: 282 SGFQADVKALQKVLSARHLIYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLG 452
>TC17393 homologue to UP|PSB1_PETHY (O82531) Proteasome subunit beta type 1
(20S proteasome alpha subunit F) (20S proteasome subunit
beta-6) , partial (35%)
Length = 576
Score = 141 bits (355), Expect = 1e-34
Identities = 71/77 (92%), Positives = 74/77 (95%)
Frame = +1
Query: 147 GSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTVFASATERDIYTGDKVEIV 206
GSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSE EAVDLVKTVFASATERDIYTGDK+EIV
Sbjct: 1 GSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSETEAVDLVKTVFASATERDIYTGDKLEIV 180
Query: 207 ILNAGGIHREFMDLRKD 223
ILNA GI RE+M+LRKD
Sbjct: 181 ILNASGIRREYMELRKD 231
>TC11663 homologue to UP|Q8VZC4 (Q8VZC4) Proteasome subunit, complete
Length = 843
Score = 82.4 bits (202), Expect = 6e-17
Identities = 44/135 (32%), Positives = 69/135 (50%)
Frame = +1
Query: 3 KQHANWSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSG 62
K + S ++ NG + VA+ G + IA+D R+ + D+ +IS + +K + SG
Sbjct: 46 KSNQTMSIFEYNGSALVAMVGKNCFAIASDRRLGVQLQTIATDFQRISKIHDKLFIGLSG 225
Query: 63 FQADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSE 122
D + L + L RH YQ + + M A L+S+ LY KRF PY+ V+ GL E
Sbjct: 226 LATDAQTLYQRLVFRHKLYQLREERDMKPETFASLVSSILYEKRFGPYFCQPVIAGLGDE 405
Query: 123 GKGCVFTYDAVGSYE 137
K + T D +G+ E
Sbjct: 406 DKPFICTMDCIGAKE 450
>TC14283 homologue to UP|O23716 (O23716) Multicatalytic endopeptidase
complex, proteasome precursor, beta subunit
(AT4G31300/F8F16_120) , partial (95%)
Length = 1117
Score = 72.4 bits (176), Expect = 6e-14
Identities = 60/220 (27%), Positives = 99/220 (44%), Gaps = 6/220 (2%)
Frame = +3
Query: 4 QHANWSPYDNNGGSCVAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGF 63
Q+ + S + G + + + + V+ AD+R STG + R KI+ L + + SG
Sbjct: 123 QNFDLSAPHSMGTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGS 302
Query: 64 QADVKALQKVLSARHLTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFN------VLG 117
AD +V+S + HQH Q+ PA ++ +N + R Y + N ++G
Sbjct: 303 AAD----SQVVSDYVRYFLHQHTIQLGQPATVKVAANLV---RLLSYNNKNFLETGLIVG 461
Query: 118 GLDSEGKGCVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLS 177
G D G +F G+ + ++ GSGS+ + F D K ++
Sbjct: 462 GWDKYEGGQIFGVPLGGTIVQQPFAIGGSGSSYLYGFFDQAWKD-------------GMT 602
Query: 178 EAEAVDLVKTVFASATERDIYTGDKVEIVILNAGGIHREF 217
+ EA DLVK + A RD +G V VI+N+ G+ R F
Sbjct: 603 KDEAEDLVKKAVSLAIARDGASGGVVRTVIINSEGVTRNF 722
>BG662283
Length = 409
Score = 72.0 bits (175), Expect = 8e-14
Identities = 40/119 (33%), Positives = 60/119 (49%)
Frame = +3
Query: 19 VAIAGSDYCVIAADTRMSTGYNILTRDYSKISHLAEKCVMASSGFQADVKALQKVLSARH 78
VA+ G + IA+D R+ + D+ +I + +K +A SG D + L + L RH
Sbjct: 15 VAMVGKNCFAIASDRRLGVQLQTVATDFQRIFKVHDKLFIALSGLATDAQTLYQRLVFRH 194
Query: 79 LTYQHQHNKQMSCPAMAQLLSNTLYYKRFFPYYSFNVLGGLDSEGKGCVFTYDAVGSYE 137
YQ + + M A L+S LY KRF PY+ V+ GL E K + T D +G+ E
Sbjct: 195 KLYQLREERDMKPETFASLVSALLYEKRFGPYFCQPVIAGLGDEDKPFICTMDCIGTKE 371
>TC11385 similar to UP|Q944Q7 (Q944Q7) At1g54990/F14C21_5, partial (12%)
Length = 448
Score = 28.1 bits (61), Expect = 1.3
Identities = 14/27 (51%), Positives = 17/27 (62%)
Frame = +1
Query: 146 SGSTLIMPFLDNQLKSPSPLLLPAQDA 172
SGSTL+ PFL + S SPL P + A
Sbjct: 244 SGSTLLFPFLSSPSPSSSPLHSPHKTA 324
>BP069765
Length = 417
Score = 27.7 bits (60), Expect = 1.7
Identities = 22/84 (26%), Positives = 34/84 (40%)
Frame = +1
Query: 126 CVFTYDAVGSYERVGYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLSEAEAVDLV 185
C+ TY V S G + L P N+ K PSP + TP+ + + L
Sbjct: 130 CIHTYSLVCQCNIA--QSGGGRNKLEKPG*SNRCKIPSPYQPTSSKGKTPIPDFRSSSLN 303
Query: 186 KTVFASATERDIYTGDKVEIVILN 209
T F S+T + +K + +N
Sbjct: 304 TT*FPSSTPKSEAETEKAQSSPMN 375
>TC15352 similar to GB|AAM70520.1|21700793|AY124811 At2g22250/T26C19.9
{Arabidopsis thaliana;}, partial (33%)
Length = 1061
Score = 26.9 bits (58), Expect = 2.8
Identities = 15/31 (48%), Positives = 18/31 (57%), Gaps = 2/31 (6%)
Frame = -1
Query: 153 PFLDNQLKSPSPLLLPAQDAV--TPLSEAEA 181
PF N++ PSP LLP D+ TPL EA
Sbjct: 581 PFHQNRISQPSPPLLPLNDSPR*TPLL*EEA 489
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 26.6 bits (57), Expect = 3.7
Identities = 12/42 (28%), Positives = 23/42 (54%)
Frame = +2
Query: 157 NQLKSPSPLLLPAQDAVTPLSEAEAVDLVKTVFASATERDIY 198
N + +P P+ ++ P SEAE +++ + +ASA +Y
Sbjct: 317 NPISTPLPVNYKLSSSMIPSSEAERMEMSRVPYASAVGSLMY 442
>BI419694
Length = 623
Score = 26.2 bits (56), Expect = 4.8
Identities = 14/36 (38%), Positives = 23/36 (63%), Gaps = 4/36 (11%)
Frame = +3
Query: 47 SKISHLAEKCVMASSGFQAD----VKALQKVLSARH 78
+K++ +A CV + F+ + VKALQ +L+ARH
Sbjct: 378 AKLAAVAALCVQYEADFRPNMSIVVKALQPLLNARH 485
>AV779816
Length = 469
Score = 25.8 bits (55), Expect = 6.3
Identities = 13/37 (35%), Positives = 20/37 (53%)
Frame = +3
Query: 140 GYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPL 176
GY+ G GS + F + KS SP+ + A +V+ L
Sbjct: 336 GYAEAGLGSPHSLTFFSSSSKSASPISIRAPVSVSKL 446
>TC15083 similar to UP|GAR1_YEAST (P28007) snoRNP protein GAR1, partial (7%)
Length = 1367
Score = 25.8 bits (55), Expect = 6.3
Identities = 14/38 (36%), Positives = 18/38 (46%)
Frame = +2
Query: 140 GYSSQGSGSTLIMPFLDNQLKSPSPLLLPAQDAVTPLS 177
G S ST P NQ+ +P PL L + + TP S
Sbjct: 476 GNGSSTRSSTA*NPLPPNQILTPPPLSLSSATSATPCS 589
>TC7894 weakly similar to UP|Q93YY0 (Q93YY0) 68 kDa protein HP68, partial
(9%)
Length = 1599
Score = 25.8 bits (55), Expect = 6.3
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = -3
Query: 54 EKCVMASSGFQADVKALQKVLSARHLTYQHQHNKQMS 90
+ C++ ++D+++ SARH Y H H+ S
Sbjct: 1588 KSCILDLKWIKSDMRSSTPCRSARHHFYSHSHSHSHS 1478
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.133 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,600,603
Number of Sequences: 28460
Number of extensions: 46252
Number of successful extensions: 232
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 232
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 232
length of query: 223
length of database: 4,897,600
effective HSP length: 87
effective length of query: 136
effective length of database: 2,421,580
effective search space: 329334880
effective search space used: 329334880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC146330.2