BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146308.4 + phase: 0
(357 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI417841 162 6e-41
TC16320 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial ... 105 1e-23
TC11659 similar to UP|Q9LR86 (Q9LR86) T23E23.24, partial (14%) 83 6e-21
TC11605 40 6e-04
BI418523 38 0.003
BP034941 33 0.096
TC12226 32 0.21
BP033684 30 0.81
CN825589 30 0.81
BP043532 29 1.1
BP084556 29 1.4
BP062688 28 1.8
TC18293 similar to UP|PWP1_HUMAN (Q13610) Periodic tryptophan pr... 28 3.1
AV768163 27 4.0
BP085870 27 5.3
CB827411 27 5.3
AV422234 27 5.3
TC10770 weakly similar to UP|RN12_HUMAN (Q9NVW2) RING finger pro... 27 5.3
BP068506 27 6.9
BP042815 27 6.9
>BI417841
Length = 617
Score = 162 bits (411), Expect = 6e-41
Identities = 80/139 (57%), Positives = 107/139 (76%)
Frame = +1
Query: 196 EQNNVKTCIVEFDGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILG 255
+ N ++C +EFDG+SKGNPG AGAGA+LR++DG+ +Y +REGVG TNN AEYR +ILG
Sbjct: 115 QSNANRSCTLEFDGSSKGNPGSAGAGAVLRAEDGSKVY-LREGVGNQTNNQAEYRGLILG 291
Query: 256 MRHALKKGFTSICIQGDSKLVCMQMKQIDGLWKVKNENLSTLYKVAKELKDKFVSFKISH 315
++HA ++G+ I ++GDS+LVC KQ++G WK +N N+++L AKELK KF SF I+H
Sbjct: 292 LKHAHEQGYQHINVKGDSQLVC---KQVEGSWKARNPNIASLCNEAKELKSKFQSFDINH 462
Query: 316 VLRDLNSEADAQANLAVNL 334
V R NSEAD QANL VNL
Sbjct: 463 VPRQYNSEADVQANLGVNL 519
>TC16320 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial (11%)
Length = 632
Score = 105 bits (262), Expect = 1e-23
Identities = 50/97 (51%), Positives = 74/97 (75%), Gaps = 1/97 (1%)
Frame = +2
Query: 249 YRAMILGMRHALKKGFTSICIQGDSKLVCMQMKQIDGLWKVKNENLSTLYKVAKELKDKF 308
YR +ILG++HA+K+G+ I ++GDS LVC Q+ GLWK+KN+N+++L AKELK+KF
Sbjct: 2 YRGLILGLKHAIKEGYKHIQVKGDSMLVC---NQVQGLWKIKNQNIASLCSEAKELKNKF 172
Query: 309 VSFKISHVLRDLNSEADAQANLAVNL-AGEAFKIINV 344
+SFKI+H+ R+ NSEAD QAN ++L AG+ ++ V
Sbjct: 173 LSFKINHIPREYNSEADVQANFGISLRAGQVEEVREV 283
>TC11659 similar to UP|Q9LR86 (Q9LR86) T23E23.24, partial (14%)
Length = 554
Score = 82.8 bits (203), Expect(2) = 6e-21
Identities = 39/65 (60%), Positives = 50/65 (76%)
Frame = -3
Query: 125 VSVYKGYSMSNETEEYLLSHGLKNALYTIRASDLKEDLFGTLVPCPFQDPSSTQGTTSNA 184
VSVYKGYS+ +TEEYL+SHGLK A Y+I A+D++E LFG LV CP+Q+P + GT S
Sbjct: 375 VSVYKGYSLPQKTEEYLVSHGLKGARYSIGAADVQEGLFGRLVACPYQEPHAYVGTASVV 196
Query: 185 DSSKK 189
+SS K
Sbjct: 195 NSSSK 181
Score = 34.3 bits (77), Expect(2) = 6e-21
Identities = 13/21 (61%), Positives = 18/21 (84%)
Frame = -1
Query: 90 ESDAFYVVRKGDVVGIYNTLT 110
E DAF+VV+KGDVVG+Y + +
Sbjct: 482 EKDAFFVVKKGDVVGVYKSFS 420
>TC11605
Length = 546
Score = 40.0 bits (92), Expect = 6e-04
Identities = 17/50 (34%), Positives = 34/50 (68%)
Frame = +3
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMR 257
DG+++G+PG +GAG ILR+ + ++ R + +G+ AE +A++L ++
Sbjct: 387 DGSARGSPGISGAGGILRNAESAVLGRFSKPLGVLCAYQAEVKAILLALK 536
>BI418523
Length = 496
Score = 37.7 bits (86), Expect = 0.003
Identities = 26/88 (29%), Positives = 46/88 (51%), Gaps = 3/88 (3%)
Frame = +1
Query: 208 DGASKGNPGKAGAGAILRSKDGNLI--YRVREGVGIATNNVAEYRAMILGMRHALKKGFT 265
DGAS+GNPG +G G + R + ++ + + G G A A+ R+++ + K
Sbjct: 226 DGASQGNPGPSGVGGVFRDANRKILGYFSLNSGNGWAYE--AKVRSILNALVFIQKFLLK 399
Query: 266 SICIQGDSKL-VCMQMKQIDGLWKVKNE 292
+I I+ DS + V M + + W++ NE
Sbjct: 400 NILIESDSTVAVEWVMSKANRPWRLSNE 483
>BP034941
Length = 589
Score = 32.7 bits (73), Expect = 0.096
Identities = 21/56 (37%), Positives = 29/56 (51%)
Frame = -3
Query: 14 TILARTTQFVANRSLHTTPHTFSFPVKSHHSLLTSFHSQFVLTTVTRCYSTRKLRK 69
TIL T + R+ P FSF KS L +FHS F+ TTVTR ++++
Sbjct: 179 TILEHTYSVL--RTFSHLPICFSFLYKSPCIFLFNFHSYFLSTTVTRKTKVERIKR 18
>TC12226
Length = 541
Score = 31.6 bits (70), Expect = 0.21
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = +1
Query: 309 VSFKISHVLRDLNSEADAQANL 330
+SF+I HVLR+ NSEADA + L
Sbjct: 1 LSFQIRHVLRNFNSEADAPSQL 66
>BP033684
Length = 557
Score = 29.6 bits (65), Expect = 0.81
Identities = 11/17 (64%), Positives = 14/17 (81%)
Frame = -1
Query: 208 DGASKGNPGKAGAGAIL 224
DGAS+GNPG +G G +L
Sbjct: 248 DGASQGNPGPSGVGGVL 198
>CN825589
Length = 544
Score = 29.6 bits (65), Expect = 0.81
Identities = 17/39 (43%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = -1
Query: 22 FVANRSLHTTPHTFSFPVKSHHSLLTSF-HSQFVLTTVT 59
F A R + TTPH SF SH+ L S HS + +VT
Sbjct: 241 FCALRHILTTPHAVSFDTTSHNPSLASIKHSSSSVRSVT 125
>BP043532
Length = 465
Score = 29.3 bits (64), Expect = 1.1
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = -2
Query: 239 VGIATNNVAEYRAMILGMRHALKKGFTSICIQGDS 273
VG+ + AE ++ G+R AL +GF+ I I+ DS
Sbjct: 464 VGVCSILHAELWGLVHGLRFALGRGFSKILIEADS 360
>BP084556
Length = 501
Score = 28.9 bits (63), Expect = 1.4
Identities = 16/49 (32%), Positives = 21/49 (42%)
Frame = +3
Query: 25 NRSLHTTPHTFSFPVKSHHSLLTSFHSQFVLTTVTRCYSTRKLRKTGSS 73
NR LH T+ HH+L T +HSQ + T + K SS
Sbjct: 48 NRQLHDILQTWILVGLLHHNLTTPYHSQLNSSIATMQQNQHSASKVSSS 194
>BP062688
Length = 536
Score = 28.5 bits (62), Expect = 1.8
Identities = 20/79 (25%), Positives = 40/79 (50%), Gaps = 1/79 (1%)
Frame = -1
Query: 255 GMRHALKKGFTSICIQGDSKLVCMQMKQI-DGLWKVKNENLSTLYKVAKELKDKFVSFKI 313
G+ H + GF + D C++++++ V+ + ++ + + + + I
Sbjct: 380 GLLHTWELGFLHVVCYVD----CLELQEVLTSTRDVQTYWHRDVIEMIRAVLARSWNVTI 213
Query: 314 SHVLRDLNSEADAQANLAV 332
+HV RD N+ ADA ANLA+
Sbjct: 212 NHVTRDGNAVADALANLAI 156
>TC18293 similar to UP|PWP1_HUMAN (Q13610) Periodic tryptophan protein 1
homolog (Keratinocyte protein IEF SSP 9502), partial
(7%)
Length = 804
Score = 27.7 bits (60), Expect = 3.1
Identities = 21/62 (33%), Positives = 33/62 (52%)
Frame = +2
Query: 192 LEVLEQNNVKTCIVEFDGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRA 251
L+VL++ V+ C+V G K GKAG ++ KDG+ +G+A N EYR
Sbjct: 257 LDVLDE--VQPCVV-LGGIEKKKKGKAGTKKSIKYKDGS---HTDSXLGLAWNK--EYRN 412
Query: 252 MI 253
++
Sbjct: 413 IL 418
>AV768163
Length = 568
Score = 27.3 bits (59), Expect = 4.0
Identities = 19/54 (35%), Positives = 25/54 (46%)
Frame = -2
Query: 4 LFSHLSSFTSTILARTTQFVANRSLHTTPHTFSFPVKSHHSLLTSFHSQFVLTT 57
LF SSFT +LAR T F+ TP FS+ + + S + H V T
Sbjct: 177 LFHPTSSFTPLLLARATSFIT-----VTPRFFSWMLGALSSSGDAIHLMEVSAT 31
>BP085870
Length = 396
Score = 26.9 bits (58), Expect = 5.3
Identities = 20/58 (34%), Positives = 25/58 (42%), Gaps = 10/58 (17%)
Frame = -3
Query: 128 YKGYSMSNETEEYLLSHGLKNALYTIRASDLKEDLFG----------TLVPCPFQDPS 175
+KG +++ E LSHGL L A LKE + G L PC DPS
Sbjct: 325 FKGINVARRLNESPLSHGLHEDLDEAPAVVLKEVVHGGCEPTHEREAALEPCA*LDPS 152
>CB827411
Length = 551
Score = 26.9 bits (58), Expect = 5.3
Identities = 9/28 (32%), Positives = 16/28 (57%)
Frame = +2
Query: 71 GSSKLHKVEAETTMNQEQNESDAFYVVR 98
GS+ +HK + NQEQ S ++++
Sbjct: 269 GSASIHKSQEPNPQNQEQKASSCIFIIK 352
>AV422234
Length = 451
Score = 26.9 bits (58), Expect = 5.3
Identities = 14/44 (31%), Positives = 22/44 (49%)
Frame = +2
Query: 4 LFSHLSSFTSTILARTTQFVANRSLHTTPHTFSFPVKSHHSLLT 47
LFS +SSF I + ++ + H +F F + HHS +T
Sbjct: 29 LFSLISSFLLIISFLNSLSLSLKPFHLNLSSFIFTLHKHHSFIT 160
>TC10770 weakly similar to UP|RN12_HUMAN (Q9NVW2) RING finger protein 12
(LIM domain interacting RING finger protein) (RING
finger LIM domain-binding protein) (R-LIM) (NY-REN-43
antigen), partial (4%)
Length = 481
Score = 26.9 bits (58), Expect = 5.3
Identities = 15/46 (32%), Positives = 25/46 (53%)
Frame = +2
Query: 287 WKVKNENLSTLYKVAKELKDKFVSFKISHVLRDLNSEADAQANLAV 332
WK+K+ N+ KVA+ DK S + L+DL D + N+++
Sbjct: 17 WKLKSLNMEVDDKVARADPDKMHS-EYEQFLKDLEENPDLRFNISL 151
>BP068506
Length = 434
Score = 26.6 bits (57), Expect = 6.9
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = -1
Query: 294 LSTLYKVAKELKDKFVSFKISHVLRDLNSEADAQANLAVN 333
LS + K LK F+ F ++HV R+LNS A +++
Sbjct: 224 LSAVLDECKHLKTCFLFFCLNHVSRNLNSATHFLAKFSLS 105
>BP042815
Length = 526
Score = 26.6 bits (57), Expect = 6.9
Identities = 17/54 (31%), Positives = 27/54 (49%), Gaps = 2/54 (3%)
Frame = -1
Query: 23 VANRSLHTTPHTFSFPVKSHHS--LLTSFHSQFVLTTVTRCYSTRKLRKTGSSK 74
++ R++ +P F FP HS L FHS+ L + + +TRK + G K
Sbjct: 238 ISQRAISNSP-PFPFPSSYFHSPSLHQRFHSEKTLLQIQQLKATRKQEEQGK*K 80
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.131 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,540,873
Number of Sequences: 28460
Number of extensions: 66010
Number of successful extensions: 442
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 437
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 439
length of query: 357
length of database: 4,897,600
effective HSP length: 91
effective length of query: 266
effective length of database: 2,307,740
effective search space: 613858840
effective search space used: 613858840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146308.4