BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144930.1 - phase: 0 /pseudo
(1322 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP065719 65 7e-11
AV427333 39 0.007
TC18379 similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich glycop... 36 0.035
TC18683 weakly similar to UP|Q8H599 (Q8H599) OJ1634_B10.18 prote... 35 0.060
TC15083 similar to UP|GAR1_YEAST (P28007) snoRNP protein GAR1, p... 35 0.060
AU251550 35 0.078
TC14274 weakly similar to GB|AAF79559.1|8778551|AC022464 F22G5.3... 34 0.13
TC14279 similar to UP|Q09085 (Q09085) Hydroxyproline-rich glycop... 34 0.17
TC18087 similar to UP|Q9LF46 (Q9LF46) 2-hydroxyphytanoyl-CoA lya... 33 0.30
TC10164 weakly similar to UP|O24099 (O24099) MtN12 protein (Frag... 33 0.39
TC12396 similar to UP|Q940Z6 (Q940Z6) At1g12810/F13K23_4, partia... 32 0.50
TC8987 similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding protein A... 32 0.50
BG662122 32 0.66
TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding p... 32 0.66
AV409036 32 0.86
TC11304 similar to PIR|T04669|T04669 serine O-acetyltransferase ... 31 1.1
TC8440 similar to UP|Q9SKU1 (Q9SKU1) At2g20760 protein (At2g2076... 31 1.1
TC15425 similar to UP|GRP2_PHAVU (P10496) Glycine-rich cell wall... 31 1.5
TC15802 similar to UP|Q9XIV1 (Q9XIV1) mRNA expressed in cucumber... 31 1.5
TC15267 similar to UP|EXTN_TOBAC (P13983) Extensin precursor (Ce... 31 1.5
>BP065719
Length = 567
Score = 65.1 bits (157), Expect = 7e-11
Identities = 42/175 (24%), Positives = 86/175 (49%), Gaps = 2/175 (1%)
Frame = +3
Query: 125 QHGSIPVTKTMEEMMEELAKELRHEIKANRGNADSFKTQDLCLVSKVDVPKKFKIPDFDR 184
+H + + + ++E+L + H N F + + V + ++P+ +K+P F +
Sbjct: 24 RHQNAGGQQNVAAVVEQLLNQ--HGFNVGFANRPHFVSAFIEEVLESELPRGWKVPKFTK 197
Query: 185 YNGLTCPQN--HIIKYVRKMGNYKDNDSLMIHCFQDSLMEDAAEWYTSLSKNDIHTFDEL 242
++G + HI +Y + G+ N++L + F SL ++A W+T+L+ +HT+ +L
Sbjct: 198 FSGDSGESTVEHIARYQIEAGDLAINENLKMKYFPSSLTKNAFTWFTTLAPRSVHTWAQL 377
Query: 243 AAAFKSHYGFNTRLKPNREFLRSLSQKKEESFREYAQRWRGAAARITPALDEEEM 297
F + F K + + L S+ +K ES +Y R+R +R + E E+
Sbjct: 378 ERIFHEQF-FRGECKVSXKDLASVKRKPAESIDDYLNRFRMLKSRCFTHVSEHEL 539
>AV427333
Length = 387
Score = 38.5 bits (88), Expect = 0.007
Identities = 30/98 (30%), Positives = 37/98 (37%), Gaps = 6/98 (6%)
Frame = +3
Query: 390 PPSYPYVPYS--QHPFFPP----FYHQYPLPPGQPQVPVNAIAQQMKQQLPVQQQQNQQA 443
PPS+PY P + HPF PP Y + P PP P + P +
Sbjct: 87 PPSHPYNPPTPPSHPFNPPPTPSHYFKSPPPPSHSFAP----PPRGHPSPPPSSPPPPSS 254
Query: 444 RPTFPPIPMLYAELLPTLLHRGHCTTRQGKPPPDPLPP 481
P PP P + P H +PPP PLPP
Sbjct: 255 HPFTPPPPHVRPPPSP---HHPITPPPHVRPPPPPLPP 359
>TC18379 similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich glycoprotein
DZ-HRGP precursor, partial (8%)
Length = 584
Score = 36.2 bits (82), Expect = 0.035
Identities = 30/101 (29%), Positives = 36/101 (34%)
Frame = +1
Query: 382 APINAAQMPPSYPYVPYSQHPFFPPFYHQYPLPPGQPQVPVNAIAQQMKQQLPVQQQQNQ 441
AP + P YP +PY QH F+PP P+ P P P A P
Sbjct: 172 APPYGMEFSP-YPSLPYPQHQFYPP-----PMAPPPPPPPAPA---------PPAPPPYW 306
Query: 442 QARPTFPPIPMLYAELLPTLLHRGHCTTRQGKPPPDPLPPR 482
A P P P L+ LP PP P PP+
Sbjct: 307 AAPPPRPYGPPLFTSTLP-------------PRPPKPAPPK 390
>TC18683 weakly similar to UP|Q8H599 (Q8H599) OJ1634_B10.18 protein, partial
(37%)
Length = 715
Score = 35.4 bits (80), Expect = 0.060
Identities = 28/98 (28%), Positives = 38/98 (38%), Gaps = 15/98 (15%)
Frame = -3
Query: 371 SAGAGQSMATVAPINAAQMPP--SYPYVPYSQHPFFP-------------PFYHQYPLPP 415
+A + T+ P A +PP S P P P FP P + PLP
Sbjct: 590 TAAPALGLPTLPPFPKATLPPLPSQPSFPKPTLPPFPAMPKFTLPPLPTMPKFTLPPLPS 411
Query: 416 GQPQVPVNAIAQQMKQQLPVQQQQNQQARPTFPPIPML 453
QPQ+P N + LP + + PT P +P L
Sbjct: 410 SQPQLP-NTLPPLPTFPLPTAPKLTLPSLPTLPTVPFL 300
>TC15083 similar to UP|GAR1_YEAST (P28007) snoRNP protein GAR1, partial (7%)
Length = 1367
Score = 35.4 bits (80), Expect = 0.060
Identities = 31/103 (30%), Positives = 36/103 (34%), Gaps = 13/103 (12%)
Frame = -2
Query: 391 PSYPYVPYSQHPFFPP----------FYHQYPLPPGQPQVPVNAIAQQMKQQLPVQQQQN 440
PS P P PF PP H P PP + NA A LPV N
Sbjct: 1276 PSVPPTPAGPTPFPPP*NSPSRPSAPSNHPTPCPPSRHAAAQNAPADAAVN-LPVSPPSN 1100
Query: 441 QQARPTFPPIPMLYAELLPTL---LHRGHCTTRQGKPPPDPLP 480
+ P PP P L P+ H + + R G P P P
Sbjct: 1099 KAVSPNLPPSPPCL--LFPSSHH*FHLQYSSARVGIYPDSPAP 977
>AU251550
Length = 329
Score = 35.0 bits (79), Expect = 0.078
Identities = 24/63 (38%), Positives = 27/63 (42%), Gaps = 1/63 (1%)
Frame = +2
Query: 390 PPSYPYVPYSQHPFFPPFYHQYPLPP-GQPQVPVNAIAQQMKQQLPVQQQQNQQARPTFP 448
PP P PY+ P PPF H +P PP G P P A M Q P +P P
Sbjct: 140 PPGPPRDPYAPAPPPPPFGHHHPPPPHGPPSPPPPA----MFYQPPPPPGPPGPPQPPHP 307
Query: 449 PIP 451
P P
Sbjct: 308 PGP 316
>TC14274 weakly similar to GB|AAF79559.1|8778551|AC022464 F22G5.35
{Arabidopsis thaliana;} , partial (16%)
Length = 1208
Score = 34.3 bits (77), Expect = 0.13
Identities = 27/85 (31%), Positives = 42/85 (48%), Gaps = 4/85 (4%)
Frame = +3
Query: 37 PIATTIPM-TTSMLPTASTDARFAMPAGFPYGLPPFFTPSTAAGTSGTANNVLIPATNAA 95
P A++ P TTSM+P AS ++ + P P ST++ S A N I T A
Sbjct: 330 PSASSSPTSTTSMIPPASANSPSSAPPAAP------TARSTSSSASLVAPNPSITPTQAP 491
Query: 96 SINATLPQTT---AAVTEPLVHTMP 117
+ TL T +++TE ++H +P
Sbjct: 492 TPTLTLTLTVTLRSSITEDILHHLP 566
>TC14279 similar to UP|Q09085 (Q09085) Hydroxyproline-rich glycoprotein
(HRGP) (Fragment), partial (57%)
Length = 941
Score = 33.9 bits (76), Expect = 0.17
Identities = 26/92 (28%), Positives = 32/92 (34%)
Frame = +1
Query: 390 PPSYPYVPYSQHPFFPPFYHQYPLPPGQPQVPVNAIAQQMKQQLPVQQQQNQQARPTFPP 449
PP P Y + P PP+Y++ P PP P P P Q+ PP
Sbjct: 31 PPPVPKPYYYKSPPPPPYYYKSP-PPPSPSPP------------PPYYYQSPPPPSHSPP 171
Query: 450 IPMLYAELLPTLLHRGHCTTRQGKPPPDPLPP 481
P Y P + PPP P PP
Sbjct: 172 PPYYYKSPPPPSPSPPPPYYYKSPPPPSPSPP 267
Score = 28.9 bits (63), Expect = 5.6
Identities = 29/110 (26%), Positives = 40/110 (36%), Gaps = 8/110 (7%)
Frame = +1
Query: 383 PINAAQMPPSYPYV---PYSQHPFFPPFYHQYPLPPGQPQVPVNAIAQQMKQQLPVQQQQ 439
P + PP Y YV P S P PP+++ P PP P K P +
Sbjct: 340 PPPTSSPPPPYHYVSPPPPSPSPP-PPYHYASPPPPSPSPAPTYI----YKSPPPPVKLP 504
Query: 440 NQQARPTFPPIPMLYAELLPTLLHRGHCTTRQGKPPP-----DPLPPRFR 484
T PP P PT +++ + PPP P PP ++
Sbjct: 505 PPPYHYTSPPPPS--PSPAPTYIYKSPPPPTKSPPPPVYIYASPPPPIYK 648
>TC18087 similar to UP|Q9LF46 (Q9LF46) 2-hydroxyphytanoyl-CoA lyase-like
protein, partial (30%)
Length = 571
Score = 33.1 bits (74), Expect = 0.30
Identities = 19/73 (26%), Positives = 36/73 (49%), Gaps = 3/73 (4%)
Frame = +2
Query: 27 AAQEQSATAIPIATTIPMTTSMLPTASTDARFAMPAGFPYGL---PPFFTPSTAAGTSGT 83
+A S T+ P+AT P+T ++ P ++ + F +PA L P+ TP + +
Sbjct: 167 SASSPSTTSSPLATPPPLTATLPPAPASTSLFPVPAASTASLASPTPWLTPGLLS*SPAP 346
Query: 84 ANNVLIPATNAAS 96
A +++ A + S
Sbjct: 347 AIRLMLAAVTSRS 385
>TC10164 weakly similar to UP|O24099 (O24099) MtN12 protein (Fragment),
partial (66%)
Length = 566
Score = 32.7 bits (73), Expect = 0.39
Identities = 24/87 (27%), Positives = 33/87 (37%), Gaps = 4/87 (4%)
Frame = +3
Query: 383 PINAAQMPPSYPYVPYSQHPFF----PPFYHQYPLPPGQPQVPVNAIAQQMKQQLPVQQQ 438
PI+ + PP Y PY +P F PPF + PP P N +P+
Sbjct: 66 PISHSPPPPGQTYPPYIPNPIFLSPPPPFPISHSPPPPVQTYPPNI-------PIPIDHS 224
Query: 439 QNQQARPTFPPIPMLYAELLPTLLHRG 465
PP+P+ +A P H G
Sbjct: 225 P--------PPVPINHAPPPPPYHHTG 281
>TC12396 similar to UP|Q940Z6 (Q940Z6) At1g12810/F13K23_4, partial (39%)
Length = 408
Score = 32.3 bits (72), Expect = 0.50
Identities = 32/124 (25%), Positives = 45/124 (35%), Gaps = 3/124 (2%)
Frame = +3
Query: 383 PINAAQMPPS---YPYVPYSQHPFFPPFYHQYPLPPGQPQVPVNAIAQQMKQQLPVQQQQ 439
P A+Q+P + Y P +P +PP + P PP Q PV P +
Sbjct: 21 PRAASQIPQTMSNYQRAPQEPYPDYPPPGYGSPYPPPQ-GYPVTQPPPGYPSAPPPPGYE 197
Query: 440 NQQARPTFPPIPMLYAELLPTLLHRGHCTTRQGKPPPDPLPPRFRSDLKCDFHQGALGHD 499
P PP+ YA ++G+ + PP P PP + H HD
Sbjct: 198 GYPPPP--PPVYAGYAARPQYEGYQGYFNQGYPQQPPPPPPPGYHC-----HHDQHHHHD 356
Query: 500 VEGC 503
GC
Sbjct: 357 SPGC 368
>TC8987 similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding protein ABP19a
precursor, partial (97%)
Length = 985
Score = 32.3 bits (72), Expect = 0.50
Identities = 11/29 (37%), Positives = 16/29 (54%), Gaps = 1/29 (3%)
Frame = +3
Query: 1022 CW-VCQRNHRRSNWIWPEACICYTRRHCE 1049
CW +C R H + NW CIC++ C+
Sbjct: 180 CWLICTRKHHKHNWCCCYPCICWSIPRCQ 266
>BG662122
Length = 386
Score = 32.0 bits (71), Expect = 0.66
Identities = 18/56 (32%), Positives = 30/56 (53%), Gaps = 2/56 (3%)
Frame = +2
Query: 466 HCTTRQGKPPPDPLPPRFRSDLK--CDFHQGALGHDVEGCYALKYIVKKLIDQGKL 519
H + G+ P PPR D C++ + + HD++ C+ LK ++KLI G+L
Sbjct: 146 HMVGKSGQS*P---PPRRGIDTTK*CEYRRSVV-HDIDDCFTLKREIEKLIKMGRL 301
>TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding protein 2,
partial (93%)
Length = 725
Score = 32.0 bits (71), Expect = 0.66
Identities = 28/101 (27%), Positives = 35/101 (33%), Gaps = 1/101 (0%)
Frame = -1
Query: 383 PINAAQMPPSYPYVPYSQHPFFPPFYHQYPLPPGQ-PQVPVNAIAQQMKQQLPVQQQQNQ 441
P+ + PPS PY P + P PP YP PP P+ P P +
Sbjct: 527 PLP*REPPPSPPYPPSRRPP*PPPPPPPYPPPPPP*PRDP----------PPPPYPPSRR 378
Query: 442 QARPTFPPIPMLYAELLPTLLHRGHCTTRQGKPPPDPLPPR 482
P +PP P +PPP P PPR
Sbjct: 377 PPPPPYPPPP---------------------RPPPPPPPPR 318
>AV409036
Length = 429
Score = 31.6 bits (70), Expect = 0.86
Identities = 15/42 (35%), Positives = 22/42 (51%)
Frame = +2
Query: 70 PFFTPSTAAGTSGTANNVLIPATNAASINATLPQTTAAVTEP 111
P TPST + + T L PAT+A + T P+ ++ T P
Sbjct: 185 PAVTPSTPSSATTTTTTSLSPATSAKTAAVTGPKAASSATSP 310
>TC11304 similar to PIR|T04669|T04669 serine O-acetyltransferase F8D20.150
- Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (24%)
Length = 775
Score = 31.2 bits (69), Expect = 1.1
Identities = 27/100 (27%), Positives = 42/100 (42%), Gaps = 12/100 (12%)
Frame = +3
Query: 390 PPSYPYVPYSQHPFFPPFYHQYPLPPGQPQVPVNAIAQQMKQQL--------PVQQQQNQ 441
PP P ++ P+F P H PP P +P +A+ + L P +++
Sbjct: 270 PPPPPPHHHNSLPYFNPTSHGLCEPPPPPPLPPP*LARSPQHALRRRPPPPPPPKRRS*T 449
Query: 442 QARPTFPPIPMLYAELLPTLLHRGHC----TTRQGKPPPD 477
+ P + + + E LP L H G C TR G+ PD
Sbjct: 450 RPPPPYSRLRLPPRESLPCLRH-GSCRPGTRTRLGRTRPD 566
>TC8440 similar to UP|Q9SKU1 (Q9SKU1) At2g20760 protein (At2g20760/F5H14.27),
partial (19%)
Length = 649
Score = 31.2 bits (69), Expect = 1.1
Identities = 17/38 (44%), Positives = 20/38 (51%), Gaps = 9/38 (23%)
Frame = +2
Query: 1026 QRNHRRSNWIWPEACI-----CYTRRHC----ERLGCH 1054
+RN +SNWI +CI CY RHC ER CH
Sbjct: 194 KRNSVQSNWICSRSCIRTQGCCYF*RHCR*TPERSPCH 307
>TC15425 similar to UP|GRP2_PHAVU (P10496) Glycine-rich cell wall structural
protein 1.8 precursor (GRP 1.8), partial (6%)
Length = 667
Score = 30.8 bits (68), Expect = 1.5
Identities = 25/74 (33%), Positives = 30/74 (39%), Gaps = 16/74 (21%)
Frame = +2
Query: 388 QMPPSYPYVPYSQ------------HPFFPPFYHQYPLPPGQPQVPVNAIA----QQMKQ 431
Q PS P PY Q HP PP+ HQ PP Q+P + A Q+ Q
Sbjct: 44 QTNPSRPQTPYYQSAEQPPVPAYGHHP--PPYGHQ---PPPPYQIPPTSAAPYPPPQVHQ 208
Query: 432 QLPVQQQQNQQARP 445
Q P + Q A P
Sbjct: 209 QPPASHEYGQPAYP 250
>TC15802 similar to UP|Q9XIV1 (Q9XIV1) mRNA expressed in cucumber hypocotyls
(Arabinogalactan protein), partial (26%)
Length = 795
Score = 30.8 bits (68), Expect = 1.5
Identities = 29/93 (31%), Positives = 39/93 (41%), Gaps = 2/93 (2%)
Frame = +2
Query: 27 AAQEQSATAIPIATTIPMT-TSMLPTASTDARFAMPAGFPYGLPPFFTPSTAAGTS-GTA 84
AA +ATA ATT P+T + P A+ PA P PP +P A T+ A
Sbjct: 218 AASPSTATAPAPATTTPVTPVTSPPPAAVPVASPPPAAVPVSSPPAKSPPAPAPTAVPVA 397
Query: 85 NNVLIPATNAASINATLPQTTAAVTEPLVHTMP 117
V P A + + T + A P+V P
Sbjct: 398 APVTTPEVPAPAPSKT-KKDAAPAPSPVVPDSP 493
>TC15267 similar to UP|EXTN_TOBAC (P13983) Extensin precursor (Cell wall
hydroxyproline-rich glycoprotein), partial (6%)
Length = 1003
Score = 30.8 bits (68), Expect = 1.5
Identities = 28/108 (25%), Positives = 36/108 (32%), Gaps = 6/108 (5%)
Frame = +2
Query: 383 PINAAQMPPSYPYVPYSQHPFFPPFYHQYPLPPGQPQVPVNAIAQQMKQQLPVQQQQNQQ 442
P PP + ++ HP P + + P P P + Q Q Q
Sbjct: 101 PTTTQSTPPP*SFHRHNLHPRHPLLHSRLGFHPRHPPPPHHHHHQATAQTTTTSQLSRLH 280
Query: 443 ARPTFPPIPMLYAELLPTLLHRGHCTTRQGKPP------PDPLPPRFR 484
L LP LLHR + +PP P PLPPR R
Sbjct: 281 PH-------RLQRHSLPGLLHRRRLLLQ--RPPHRPTIHPHPLPPRLR 397
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.332 0.143 0.464
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,292,783
Number of Sequences: 28460
Number of extensions: 426210
Number of successful extensions: 3379
Number of sequences better than 10.0: 84
Number of HSP's better than 10.0 without gapping: 3133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3325
length of query: 1322
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1221
effective length of database: 2,023,140
effective search space: 2470253940
effective search space used: 2470253940
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC144930.1