BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC141863.8 - phase: 0
(271 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC13498 homologue to UP|PSB5_SPIOL (O24361) Proteasome subunit b... 318 8e-88
TC14283 homologue to UP|O23716 (O23716) Multicatalytic endopepti... 94 2e-20
TC16454 similar to UP|O81152 (O81152) 20S proteasome beta subuni... 80 5e-16
TC14699 homologue to UP|PSA5_ORYSA (Q9LSU1) Proteasome subunit a... 70 5e-13
TC11663 homologue to UP|Q8VZC4 (Q8VZC4) Proteasome subunit, comp... 65 9e-12
TC15875 similar to UP|PS21_ARATH (O23714) Proteasome subunit bet... 57 3e-09
TC18392 homologue to UP|PSA2_ORYSA (Q9LSU2) Proteasome subunit a... 41 2e-04
TC12945 similar to UP|PSA2_ARATH (O23708) Proteasome subunit alp... 39 0.001
TC14473 similar to UP|Q8H1Y2 (Q8H1Y2) 20S proteasome alpha 6 sub... 36 0.006
BG662283 35 0.018
TC8154 28 2.2
BP060445 28 2.2
TC20190 similar to PIR|F96767|F96767 proteinase IV F2P9.14 [impo... 27 2.8
TC10061 similar to GB|AAO42791.1|28416521|BT004545 AT3g29240/MXO... 26 6.3
AV779401 26 8.2
>TC13498 homologue to UP|PSB5_SPIOL (O24361) Proteasome subunit beta type 5
precursor (20S proteasome subunit E) (Proteasome
epsilon chain) , partial (53%)
Length = 597
Score = 318 bits (814), Expect = 8e-88
Identities = 155/169 (91%), Positives = 162/169 (95%)
Frame = +2
Query: 2 LDTTALESFPSLISHGINVAGEISAPPSFQLPNSNDFDGFVKETIQMVKPAKGTTTLAFI 61
LDTT LESFP L++ G +V GE +APPSF+LPNSNDFDGFVKE IQMVKPAKGTTTLAFI
Sbjct: 89 LDTTGLESFPKLVAPGNDVVGEFTAPPSFELPNSNDFDGFVKEAIQMVKPAKGTTTLAFI 268
Query: 62 FKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHE 121
FK+GVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHE
Sbjct: 269 FKDGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHE 448
Query: 122 LANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDS 170
LANKRRISVTGASK+LANILYSYRGMGLSVGTMIAGWDETGPGLYYVDS
Sbjct: 449 LANKRRISVTGASKMLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDS 595
>TC14283 homologue to UP|O23716 (O23716) Multicatalytic endopeptidase
complex, proteasome precursor, beta subunit
(AT4G31300/F8F16_120) , partial (95%)
Length = 1117
Score = 94.0 bits (232), Expect = 2e-20
Identities = 61/205 (29%), Positives = 100/205 (48%), Gaps = 3/205 (1%)
Frame = +3
Query: 54 GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 113
GTT + + GV++ ADSR S G Y+++++ KI ++ + +G AAD Q +
Sbjct: 156 GTTIIGVTYNGGVVLGADSRTSTGVYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYV 335
Query: 114 GIKCRLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGG 173
H + + +V A+ L+ + Y+ + L G ++ GWD+ G + GG
Sbjct: 336 RYFLHQHTIQLGQPATVKVAANLVRLLSYNNKNF-LETGLIVGGWDKYEGGQIFGVPLGG 512
Query: 174 RLKGNRFSVG-SGSPYAYGVLDSGYKYDMSVEEASELARRAIYHATFRDGASGGVASVYY 232
+ F++G SGS Y YG D +K M+ +EA +L ++A+ A RDGASGGV
Sbjct: 513 TIVQQPFAIGGSGSSYLYGFFDQAWKDGMTKDEAEDLVKKAVSLAIARDGASGGVVRTVI 692
Query: 233 VGPTGWKK--LSGDDVGELHYHYNP 255
+ G + GD + H P
Sbjct: 693 INSEGVTRNFYPGDQLPLWHEELEP 767
>TC16454 similar to UP|O81152 (O81152) 20S proteasome beta subunit PBB1
(20S proteasome beta subunit, multicatalytic
endopeptidase) (At3g27430) , complete
Length = 1277
Score = 79.7 bits (195), Expect = 5e-16
Identities = 53/177 (29%), Positives = 89/177 (49%)
Frame = +1
Query: 54 GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 113
GTT + +F++GV++ AD+RA+ G ++ ++ +KI + P + AG AAD + +
Sbjct: 205 GTTIVGLVFQDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDMV 384
Query: 114 GIKCRLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGG 173
+ +LH R + A LL L++Y+G +S ++ G D TGP L+ + G
Sbjct: 385 SSQLQLHRYHTGRESRIVTALTLLKRHLFNYQGQ-VSAALVLGGVDATGPHLHTIYPHGS 561
Query: 174 RLKGNRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRAIYHATFRDGASGGVASV 230
++GSGS A V +S YK ++ +E ++ AI F D SG V
Sbjct: 562 TDTLPFATMGSGSLAAMSVFESKYKESLTRDEGIKIVVEAICAGVFNDLGSGSNVDV 732
>TC14699 homologue to UP|PSA5_ORYSA (Q9LSU1) Proteasome subunit alpha type 5
(20S proteasome alpha subunit E) (20S proteasome
subunit alpha-5) , complete
Length = 1044
Score = 69.7 bits (169), Expect = 5e-13
Identities = 50/177 (28%), Positives = 89/177 (50%), Gaps = 8/177 (4%)
Frame = +1
Query: 42 VKETIQMVKPAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAG 101
V+ I+ +K G+T + KEGV++A + R + + SV+KI+EI+ ++ M+G
Sbjct: 151 VEYAIEAIK--LGSTAIGLKTKEGVVLAVEKRIT-SPLLEPSSVEKIMEIDAHIGCAMSG 321
Query: 102 GAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLANILYSYRGMG--------LSVGT 153
AD + + ++ + H + ++V ++ L ++ + G G V
Sbjct: 322 LIADARTLVEHARVETQNHRFSYGEPMTVESTTQALCDLALRF-GEGDEESMSRPFGVSL 498
Query: 154 MIAGWDETGPGLYYVDSEGGRLKGNRFSVGSGSPYAYGVLDSGYKYDMSVEEASELA 210
+IAG DE GP LYY D G + N ++GSGS A L Y +++++EA +A
Sbjct: 499 LIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQYNKELTLQEAETIA 669
>TC11663 homologue to UP|Q8VZC4 (Q8VZC4) Proteasome subunit, complete
Length = 843
Score = 65.5 bits (158), Expect = 9e-12
Identities = 53/189 (28%), Positives = 90/189 (47%), Gaps = 2/189 (1%)
Frame = +1
Query: 54 GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 113
G+ +A + K +A+D R + + ++I +I+ + ++G A D Q ++ L
Sbjct: 82 GSALVAMVGKNCFAIASDRRLGVQLQTIATDFQRISKIHDKLFIGLSGLATDAQTLYQRL 261
Query: 114 GIKCRLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAG-WDETGPGLYYVDSEG 172
+ +L++L +R + + L+++ILY R +IAG DE P + +D G
Sbjct: 262 VFRHKLYQLREERDMKPETFASLVSSILYEKRFGPYFCQPVIAGLGDEDKPFICTMDCIG 441
Query: 173 GRLKGNRFSV-GSGSPYAYGVLDSGYKYDMSVEEASELARRAIYHATFRDGASGGVASVY 231
+ F V GS S YG ++ +K DM EE E +A+ + RD SG VY
Sbjct: 442 AKELAKDFVVAGSASESLYGACEAMFKPDMEPEELFETISQALLSSVDRDCLSGWGGHVY 621
Query: 232 YVGPTGWKK 240
V PT K+
Sbjct: 622 VVTPTEVKE 648
>TC15875 similar to UP|PS21_ARATH (O23714) Proteasome subunit beta type 2-1
(20S proteasome alpha subunit D1) , partial (97%)
Length = 992
Score = 57.4 bits (137), Expect = 3e-09
Identities = 43/156 (27%), Positives = 73/156 (46%), Gaps = 2/156 (1%)
Frame = +1
Query: 67 MVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKR 126
+VAAD+ A + KI+ ++ + L +G A D + + L++ N
Sbjct: 109 IVAADTSAVHSILVHKSDEDKIMLLDSHKLVAASGEAGDRVQFTEYIQKNVALYQFRNGI 288
Query: 127 RISVTGASKLL-ANILYSYRGMGLSVGTMIAGWD-ETGPGLYYVDSEGGRLKGNRFSVGS 184
++ A+ + + R SV ++AG+D ETGP LYY+D K + + G
Sbjct: 289 PLTTAAAANFTRGELAIALRKNPYSVNILLAGYDKETGPSLYYIDYIATLHKLEKGAFGY 468
Query: 185 GSPYAYGVLDSGYKYDMSVEEASELARRAIYHATFR 220
GS ++ ++D Y MSVEEA +L + I+ R
Sbjct: 469 GSYFSLSMMDRHYHSGMSVEEAIDLVDKCIHEIRSR 576
>TC18392 homologue to UP|PSA2_ORYSA (Q9LSU2) Proteasome subunit alpha type 2
(20S proteasome alpha subunit B) (20S proteasome
subunit alpha-2) , partial (71%)
Length = 548
Score = 40.8 bits (94), Expect = 2e-04
Identities = 36/159 (22%), Positives = 63/159 (38%), Gaps = 4/159 (2%)
Frame = +1
Query: 29 SFQLPNSNDFDGFVKETIQMVKPAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKI 88
SF L + V+ + G T+L GV++A + + + SV+KI
Sbjct: 61 SFSLTTFSPSGKLVQIEHALTAVGSGQTSLGIKAANGVVIATEKKLP-SILVDEASVQKI 237
Query: 89 IEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLANILYSYRGMG 148
+ P + +G D + R + + K I VT + +A ++ + G
Sbjct: 238 QLLTPNIGVVYSGMGPDFRVLVRKSRKQAEQYHRLYKEPIPVTQLVREVAAVMQEFTQSG 417
Query: 149 ----LSVGTMIAGWDETGPGLYYVDSEGGRLKGNRFSVG 183
V ++AG+D+ GP LY VD G ++G
Sbjct: 418 GVRPFGVSLLVAGFDDNGPQLYQVDPSGSYFSWKASAMG 534
>TC12945 similar to UP|PSA2_ARATH (O23708) Proteasome subunit alpha type 2
(20S proteasome alpha subunit B) , partial (93%)
Length = 744
Score = 38.5 bits (88), Expect = 0.001
Identities = 32/135 (23%), Positives = 53/135 (38%), Gaps = 4/135 (2%)
Frame = +3
Query: 80 ISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLAN 139
+ SV+KI + P + +G D + R + + K I VT + +A
Sbjct: 225 VDETSVQKIQLLTPNIGVVYSGMGPDFRVLVRKSRKQAEQYHRLYKEPIPVTQLVREVAA 404
Query: 140 ILYSYRGMG----LSVGTMIAGWDETGPGLYYVDSEGGRLKGNRFSVGSGSPYAYGVLDS 195
++ + G V +IAG+D+ GP LY V G ++G A L
Sbjct: 405 VMQEFTQSGGVRPFGVSLLIAGFDDNGPQLYQVXPSGSYFSWKASAMGKNVSNAKTFLXK 584
Query: 196 GYKYDMSVEEASELA 210
Y M +++A A
Sbjct: 585 KYTDXMXLDDAXHTA 629
>TC14473 similar to UP|Q8H1Y2 (Q8H1Y2) 20S proteasome alpha 6 subunit,
partial (86%)
Length = 1302
Score = 36.2 bits (82), Expect = 0.006
Identities = 40/162 (24%), Positives = 75/162 (45%), Gaps = 5/162 (3%)
Frame = +3
Query: 42 VKETIQMVKPAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAG 101
V+ ++ VK +G+ + K V++A ++A+ +SS KKI +++ ++ +AG
Sbjct: 201 VEYAMEAVK--QGSAAIGLRSKTHVVLACVNKAN--SELSSHQ-KKIFKVDDHIGVAIAG 365
Query: 102 GAADCQFWHRNLGIKCRLHELANKR-----RISVTGASKLLANILYSYRGMGLSVGTMIA 156
AD + R + +C + + R+ V A K S++ VG ++
Sbjct: 366 LTADGRVLSRYMRSECINYSYTYESPLPVGRLVVHLADKAQVCTQRSWK-RPYGVGLLVG 542
Query: 157 GWDETGPGLYYVDSEGGRLKGNRFSVGSGSPYAYGVLDSGYK 198
G DE+G LYY G + F++GS S A L+ ++
Sbjct: 543 GLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFE 668
>BG662283
Length = 409
Score = 34.7 bits (78), Expect = 0.018
Identities = 31/131 (23%), Positives = 61/131 (45%), Gaps = 2/131 (1%)
Frame = +3
Query: 58 LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 117
+A + K +A+D R + + ++I +++ + ++G A D Q ++ L +
Sbjct: 15 VAMVGKNCFAIASDRRLGVQLQTVATDFQRIFKVHDKLFIALSGLATDAQTLYQRLVFRH 194
Query: 118 RLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAG-WDETGPGLYYVDSEGGRLK 176
+L++L +R + + L++ +LY R +IAG DE P + +D G +
Sbjct: 195 KLYQLREERDMKPETFASLVSALLYEKRFGPYFCQPVIAGLGDEDKPFICTMDCIGTKEL 374
Query: 177 GNRFSV-GSGS 186
F V GS S
Sbjct: 375 AKDFVVAGSAS 407
>TC8154
Length = 503
Score = 27.7 bits (60), Expect = 2.2
Identities = 26/92 (28%), Positives = 38/92 (41%), Gaps = 4/92 (4%)
Frame = -1
Query: 65 GVMVAA-DSRASMGGYISSQSVKKIIEINPYMLGTMAGGA---ADCQFWHRNLGIKCRLH 120
GVMV +A GG + + +K + G G A C+ H + I+CR
Sbjct: 482 GVMVVELTGKAPEGGSVGGRRRRKDDPEYGFCCGAAQGFQRY*ARCERRHEDTRIECRSL 303
Query: 121 ELANKRRISVTGASKLLANILYSYRGMGLSVG 152
+RR+ + LL L+ M LSVG
Sbjct: 302 RHRRRRRVIILCFHVLLPLFLFMELAMVLSVG 207
>BP060445
Length = 439
Score = 27.7 bits (60), Expect = 2.2
Identities = 37/128 (28%), Positives = 46/128 (35%), Gaps = 8/128 (6%)
Frame = -3
Query: 110 HRNLGIKCRLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGW--DETGPGLYY 167
H NL I RRIS +S + I + R G+S T+ W +E G
Sbjct: 413 HHNLKITLSWGMAIQVRRISTELSSYMNNIIRFEMRNRGVSQITLKYNWNPEERGRRRVL 234
Query: 168 VDSEGGRLKGNRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRAIYHATFRD------ 221
GG R SVG G L V+ EL +R+
Sbjct: 233 ARENGG---SPRVSVGGGLE-----LKRRMNLADEVDGVGELGLEGNLRGFWREGFGFSS 78
Query: 222 GASGGVAS 229
GASGGVAS
Sbjct: 77 GASGGVAS 54
>TC20190 similar to PIR|F96767|F96767 proteinase IV F2P9.14 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(13%)
Length = 570
Score = 27.3 bits (59), Expect = 2.8
Identities = 18/50 (36%), Positives = 23/50 (46%), Gaps = 4/50 (8%)
Frame = +3
Query: 143 SYRGMGLSVGTMIAGWDETGP----GLYYVDSEGGRLKGNRFSVGSGSPY 188
S G+ L VG+ + G D T GL + D R+ G F GSPY
Sbjct: 111 SLPGILLGVGSSLVGVDRTLKELLQGLTFSDGVQARMDGIMFQTLEGSPY 260
>TC10061 similar to GB|AAO42791.1|28416521|BT004545 AT3g29240/MXO21_9
{Arabidopsis thaliana;}, partial (26%)
Length = 537
Score = 26.2 bits (56), Expect = 6.3
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = -1
Query: 228 ASVYYVGPTGWKKLSGDDVGELHYHY 253
A++ Y+ P W + S V +LHY Y
Sbjct: 423 ATILYLMPANWSEHSKQGVLDLHYKY 346
>AV779401
Length = 483
Score = 25.8 bits (55), Expect = 8.2
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = -2
Query: 112 NLGIKCRLHELANKRRISVTGASKLLANILYSYR 145
N GIK R+H +NKR +T + L IL+S R
Sbjct: 173 NKGIKIRVHVCSNKRSSPITTQPQSL-EILHSQR 75
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.135 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,188,124
Number of Sequences: 28460
Number of extensions: 48376
Number of successful extensions: 286
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 283
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 284
length of query: 271
length of database: 4,897,600
effective HSP length: 89
effective length of query: 182
effective length of database: 2,364,660
effective search space: 430368120
effective search space used: 430368120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)
Medicago: description of AC141863.8