BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC141109.8 + phase: 0
(509 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV776921 170 4e-43
BP039737 144 3e-35
BP075001 123 6e-29
BI417718 90 1e-18
AV413975 81 5e-16
AV411197 66 1e-11
BG662269 62 2e-10
TC11559 similar to UP|Q9SXT3 (Q9SXT3) Multidrug resistance prote... 62 2e-10
AV418183 55 3e-08
AV767093 54 6e-08
AU089197 42 3e-04
BG662257 41 5e-04
TC10604 similar to AAQ89671 (AAQ89671) At4g33460, partial (36%) 39 0.002
AV417384 35 0.038
BI420290 35 0.038
BP058734 33 0.14
AW720269 31 0.42
TC18597 30 1.2
TC16623 weakly similar to UP|TAP2_HUMAN (Q03519) Antigen peptide... 29 2.1
BP036801 28 2.7
>AV776921
Length = 435
Score = 170 bits (431), Expect = 4e-43
Identities = 84/129 (65%), Positives = 105/129 (81%)
Frame = +2
Query: 365 NVEFVYPSRPESVILNDFCLKVPSGKTVALVGGSGSGKSTVVSLLQRFYDPIGGEILLDG 424
+V F YPSRP+ ILN CL +PSGK VALVGGSGSGKSTV+SL++RFY+P+ G+ILLDG
Sbjct: 2 DVCFSYPSRPDVEILNKLCLDIPSGKIVALVGGSGSGKSTVISLIERFYEPLSGDILLDG 181
Query: 425 VAIHKLQLKWLRSQMGLVSQEPALFATSIKENILFGREDATYEEIVDAAKASNAHNFISM 484
I L LKWLR Q+GLV+QEPALFATSIKENIL+G+++AT EE+ A K S+A +FI+
Sbjct: 182 NDIRDLDLKWLRQQIGLVNQEPALFATSIKENILYGKDNATLEELKRAVKLSDAQSFINN 361
Query: 485 LPQGYDTQV 493
LP+ +TQV
Sbjct: 362 LPERLETQV 388
>BP039737
Length = 543
Score = 144 bits (364), Expect = 3e-35
Identities = 68/168 (40%), Positives = 109/168 (64%), Gaps = 1/168 (0%)
Frame = +3
Query: 230 SIRTVYSFVGESKTLAAFSNALEGSVKLGLKQGLAKGLAIGSN-GVVYAIWSLIFYYGSI 288
S VYS+VGESK L ++S+A++ ++KLG K G+AKGL +G G+ W+L+F+Y +
Sbjct: 39 SSSNVYSYVGESKALNSYSDAIQNTLKLGYKAGMAKGLGLGCTYGIACMSWALVFWYAGV 218
Query: 289 MVMYHGAKGGTVFVVGVTLAIGGLAFGTCFSNVRYFAEASVAGERIMEVIKRVPTIDSEN 348
+ GG F + +GG++ G FSN+ F++ AG ++ME+IK+ PTI +
Sbjct: 219 FIRNGQTDGGKAFTAIFSAIVGGMSLGQSFSNLGAFSKGKAAGYKLMEIIKQKPTIIEDL 398
Query: 349 MEGEIIEKVLGEVEFNNVEFVYPSRPESVILNDFCLKVPSGKTVALVG 396
+G+ +++V G +EF +V F YPSRP+ +I +F + P+GKTVA+VG
Sbjct: 399 SDGKCLDEVNGNIEFKDVTFSYPSRPDVIIFRNFSIFFPAGKTVAVVG 542
>BP075001
Length = 403
Score = 123 bits (309), Expect = 6e-29
Identities = 61/134 (45%), Positives = 91/134 (67%)
Frame = +2
Query: 221 GTIAEQAISSIRTVYSFVGESKTLAAFSNALEGSVKLGLKQGLAKGLAIGSNGVVYAIWS 280
G++AEQ ISSIRTVY +V E +TL FS+ALE S++LG+KQG KG+ IGS G++YA W+
Sbjct: 2 GSVAEQCISSIRTVYPYVSEKQTLNRFSSALEKSMELGIKQGQTKGVVIGSIGLLYATWA 181
Query: 281 LIFYYGSIMVMYHGAKGGTVFVVGVTLAIGGLAFGTCFSNVRYFAEASVAGERIMEVIKR 340
+ GS++V G GG VFV + + GG++ + + + EASVAG RI+E+I R
Sbjct: 182 FQSWAGSVLVRTKGESGGQVFVAQICIIWGGISLMSALPKLAFIIEASVAGTRIIEMIDR 361
Query: 341 VPTIDSENMEGEII 354
P+I++ +G ++
Sbjct: 362 KPSINANGEKGRLL 403
>BI417718
Length = 515
Score = 89.7 bits (221), Expect = 1e-18
Identities = 56/160 (35%), Positives = 85/160 (53%), Gaps = 1/160 (0%)
Frame = +2
Query: 247 FSNALEGSVKLGLKQGLAKGLAIG-SNGVVYAIWSLIFYYGSIMVMYHGAKGGTVFVVGV 305
+S EG VK G+++GL G+ G S +++++++ F+ G+ V A VF V
Sbjct: 44 YSRKCEGPVKTGIQRGLISGIGFGVSFFLLFSVYATTFHVGARFVGAGMASFSDVFQVLF 223
Query: 306 TLAIGGLAFGTCFSNVRYFAEASVAGERIMEVIKRVPTIDSENMEGEIIEKVLGEVEFNN 365
L + + N ++A + I E+I R ID + G ++ G++EF +
Sbjct: 224 ALTMAAIGISRRAPNS---SKAKIVTASIFEIIDRKSKIDPCDESGSTLDSTKGKIEFCH 394
Query: 366 VEFVYPSRPESVILNDFCLKVPSGKTVALVGGSGSGKSTV 405
V F YPSRP+ I D L + +G TVALVG SGSGKSTV
Sbjct: 395 VSFKYPSRPDIQIFPDLSLTIHAGTTVALVGESGSGKSTV 514
>AV413975
Length = 343
Score = 80.9 bits (198), Expect = 5e-16
Identities = 41/100 (41%), Positives = 65/100 (65%)
Frame = +2
Query: 326 EASVAGERIMEVIKRVPTIDSENMEGEIIEKVLGEVEFNNVEFVYPSRPESVILNDFCLK 385
+A+ A R+ +++ RV ++ + + ++ GEVE ++V F YPSRP +L +K
Sbjct: 47 KAAGASRRVFQIMDRVSSMSKSGTKCPLGDQD-GEVELDDVWFSYPSRPSHPVLKGITMK 223
Query: 386 VPSGKTVALVGGSGSGKSTVVSLLQRFYDPIGGEILLDGV 425
+ G VALVG SG GK+T+ +L++RFYDP G+ILL+GV
Sbjct: 224 LHPGSKVALVGPSGGGKTTIANLIERFYDPTKGKILLNGV 343
>AV411197
Length = 391
Score = 66.2 bits (160), Expect = 1e-11
Identities = 28/55 (50%), Positives = 43/55 (77%)
Frame = +3
Query: 441 LVSQEPALFATSIKENILFGREDATYEEIVDAAKASNAHNFISMLPQGYDTQVSV 495
L+ QEP +F+T+I+ENI++ R +A+ E+ +AA+ +NAH+FIS LP GYDT V +
Sbjct: 54 LIQQEPIIFSTTIRENIIYARHNASEAEMKEAARIANAHHFISSLPHGYDTHVGM 218
>BG662269
Length = 283
Score = 62.4 bits (150), Expect = 2e-10
Identities = 31/55 (56%), Positives = 41/55 (74%), Gaps = 1/55 (1%)
Frame = +2
Query: 427 IHKLQLKWLRSQMGLVSQEPALFATSIKENILFGR-EDATYEEIVDAAKASNAHN 480
I LQ+KWLR QMG+VSQEP LF +I+ NI +G+ +AT EI+ AA+ +NAHN
Sbjct: 20 IQTLQVKWLRQQMGMVSQEPVLFNETIRANIAYGKGGNATEAEIIAAAELANAHN 184
>TC11559 similar to UP|Q9SXT3 (Q9SXT3) Multidrug resistance protein
(Fragment), partial (41%)
Length = 460
Score = 62.0 bits (149), Expect = 2e-10
Identities = 31/83 (37%), Positives = 49/83 (58%)
Frame = +3
Query: 378 ILNDFCLKVPSGKTVALVGGSGSGKSTVVSLLQRFYDPIGGEILLDGVAIHKLQLKWLRS 437
IL L +P G V ++G SGSGKST++ L R ++P + LD I L + LR
Sbjct: 135 ILKGIHLDIPKGVIVGVIGPSGSGKSTLLRALNRLWEPPSASVFLDARDICHLDVLSLRR 314
Query: 438 QMGLVSQEPALFATSIKENILFG 460
++G++ Q PALF ++ +N+ +G
Sbjct: 315 KVGMLFQLPALFEGTVADNVRYG 383
>AV418183
Length = 298
Score = 55.1 bits (131), Expect = 3e-08
Identities = 30/71 (42%), Positives = 44/71 (61%), Gaps = 1/71 (1%)
Frame = +3
Query: 424 GVAIHKLQLKWLRSQMGLVSQEPALFATSIKENILFGREDATYE-EIVDAAKASNAHNFI 482
GV + ++ + L ++ +VSQEP LF SI+ENI +G + +I +AAK +NAH FI
Sbjct: 3 GVPLAEISHRHLHRKISIVSQEPTLFNCSIEENIAYGFDGKLNSVDIENAAKMANAHEFI 182
Query: 483 SMLPQGYDTQV 493
S P+ Y T V
Sbjct: 183 SKFPEKYQTFV 215
>AV767093
Length = 603
Score = 53.9 bits (128), Expect = 6e-08
Identities = 28/75 (37%), Positives = 47/75 (62%), Gaps = 1/75 (1%)
Frame = +1
Query: 359 GEVEFNNVEFVY-PSRPESVILNDFCLKVPSGKTVALVGGSGSGKSTVVSLLQRFYDPIG 417
G +E N+++ Y P+ P ++L L V G+ + +VG +GSGKST++ +L R +P
Sbjct: 379 GSIELNSLQVRYRPNTP--LVLKGISLTVQGGEKIGVVGRTGSGKSTLIQVLFRLIEPSA 552
Query: 418 GEILLDGVAIHKLQL 432
G+I++DG+ I L L
Sbjct: 553 GKIIIDGINICTLGL 597
>AU089197
Length = 658
Score = 41.6 bits (96), Expect = 3e-04
Identities = 25/76 (32%), Positives = 40/76 (51%), Gaps = 1/76 (1%)
Frame = +1
Query: 359 GEVEFNNVEFVY-PSRPESVILNDFCLKVPSGKTVALVGGSGSGKSTVVSLLQRFYDPIG 417
G +E + ++ Y P+ P ++L D K + + +GSGKST++ L P+
Sbjct: 352 GMIELHKLQVQYDPAAP--IVLKDVTFIFSGQKKIGIXXRTGSGKSTLLQALF*IVXPLS 525
Query: 418 GEILLDGVAIHKLQLK 433
G IL+DGV I +L K
Sbjct: 526 GCILIDGVXIPRLACK 573
>BG662257
Length = 336
Score = 40.8 bits (94), Expect = 5e-04
Identities = 20/34 (58%), Positives = 24/34 (69%)
Frame = +1
Query: 460 GREDATYEEIVDAAKASNAHNFISMLPQGYDTQV 493
G + AT EI+ AA+ +NAH FIS L QGYDT V
Sbjct: 19 GGDAATEAEIIAAAELANAHKFISSLQQGYDTVV 120
>TC10604 similar to AAQ89671 (AAQ89671) At4g33460, partial (36%)
Length = 498
Score = 39.3 bits (90), Expect = 0.002
Identities = 23/86 (26%), Positives = 44/86 (50%), Gaps = 2/86 (2%)
Frame = +2
Query: 340 RVPTIDSENMEGEIIEKVLGEVEFNNVEFVYPSRPESVI--LNDFCLKVPSGKTVALVGG 397
R T+ + + +E V + N+ F + +R + + L D L++PSG+ L+G
Sbjct: 86 RCSTLPTNTIRSNSVENVA--IVGRNLNFSFTARQTNDVRVLRDCSLRIPSGQFWMLLGP 259
Query: 398 SGSGKSTVVSLLQRFYDPIGGEILLD 423
+G GKST++ +L P G + ++
Sbjct: 260 NGCGKSTLLKILAGLLAPTSGTVYVN 337
>AV417384
Length = 380
Score = 34.7 bits (78), Expect = 0.038
Identities = 22/58 (37%), Positives = 29/58 (49%)
Frame = +2
Query: 364 NNVEFVYPSRPESVILNDFCLKVPSGKTVALVGGSGSGKSTVVSLLQRFYDPIGGEIL 421
NNV + R IL + + G + L G +GSGKST + +L F P GEIL
Sbjct: 212 NNVSCM---RNAQQILRHVNVSLHDGGALVLTGANGSGKSTFLRMLAGFSRPSAGEIL 376
>BI420290
Length = 398
Score = 34.7 bits (78), Expect = 0.038
Identities = 18/48 (37%), Positives = 27/48 (55%)
Frame = +2
Query: 359 GEVEFNNVEFVYPSRPESVILNDFCLKVPSGKTVALVGGSGSGKSTVV 406
G + F N++ Y SV+ N C P K + +VG +GSGKST++
Sbjct: 257 GTICFKNLQIRYAEHLPSVLKNITCT-FPGRKKIGVVGRTGSGKSTLI 397
>BP058734
Length = 453
Score = 32.7 bits (73), Expect = 0.14
Identities = 24/57 (42%), Positives = 32/57 (56%), Gaps = 2/57 (3%)
Frame = -3
Query: 133 LHVTSTTDVITSVSSDSLVIQD-VLS-DKVPNFLVNASRFLSSNIVAFALLWRLAIV 187
L ++S T T+ S L + D VLS K+P + S FLS +VAF +LW L IV
Sbjct: 214 LFLSSMTGSPTTTSFGPLSLADGVLSWSKLPTKIAKGSTFLSRLMVAFRILW*LRIV 44
>AW720269
Length = 524
Score = 31.2 bits (69), Expect = 0.42
Identities = 30/97 (30%), Positives = 48/97 (48%), Gaps = 10/97 (10%)
Frame = +3
Query: 373 RPESV-ILNDFCLKVPSGKTVALVGGSGSGKSTVVSLL-----QRFYDPIGGEILLDGVA 426
+P+ V IL S + VA+VG SG+GKST++ ++ R +DP ++
Sbjct: 36 KPKPVNILKSVSFVARSSEIVAVVGPSGTGKSTLLRIIAGRVKDRDFDP-------KTIS 194
Query: 427 IHKLQL---KWLRSQMGLVSQEPALF-ATSIKENILF 459
I+ + LR G V+QE L ++KE +LF
Sbjct: 195 INDHPMTSPAQLRKICGFVAQEDNLLPLLTVKETLLF 305
>TC18597
Length = 550
Score = 29.6 bits (65), Expect = 1.2
Identities = 25/109 (22%), Positives = 47/109 (42%), Gaps = 5/109 (4%)
Frame = +2
Query: 93 SFFVCF-----LEGYCWTRTGERQAARMRVRYLKAVLRQEVSYFDLHVTSTTDVITSVSS 147
++F+CF L W + ER +++ ++ + +LR ++ +F+ + V+ + S
Sbjct: 188 AYFLCFAADPALNIVKWNKGNERH*SQIMIKLCQMLLRLQLHFFE--TITNVSVLLAPRS 361
Query: 148 DSLVIQDVLSDKVPNFLVNASRFLSSNIVAFALLWRLAIVGFPFMVLLV 196
V F V SRFL S ++ F L W F F ++
Sbjct: 362 YF*V-----------FAVFLSRFLFSFVLDFNLTWERLFPRFSFYEFMI 475
>TC16623 weakly similar to UP|TAP2_HUMAN (Q03519) Antigen peptide
transporter 2 (APT2) (Peptide transporter TAP2) (Peptide
transporter PSF2) (Peptide supply factor 2) (PSF-2)
(Peptide transporter involved in antigen processing 2),
partial (4%)
Length = 530
Score = 28.9 bits (63), Expect = 2.1
Identities = 17/49 (34%), Positives = 25/49 (50%), Gaps = 2/49 (4%)
Frame = +1
Query: 378 ILNDFCLKVPSGKTVALVGGSGSGKSTVVSLL--QRFYDPIGGEILLDG 424
+L L + G+ AL+G +GSGKST +L Y+ GG + G
Sbjct: 211 LLKGLNLTINRGEVHALMGANGSGKSTFAKVLVGHPDYEVTGGRAVYKG 357
>BP036801
Length = 540
Score = 28.5 bits (62), Expect = 2.7
Identities = 14/38 (36%), Positives = 21/38 (54%)
Frame = -1
Query: 388 SGKTVALVGGSGSGKSTVVSLLQRFYDPIGGEILLDGV 425
+G VGG G+G + V L + +GGE++ DGV
Sbjct: 384 AGLEEGAVGGEGAGGAPCVGLDGERAESVGGEVVEDGV 271
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.138 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,734,140
Number of Sequences: 28460
Number of extensions: 93994
Number of successful extensions: 560
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 557
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 558
length of query: 509
length of database: 4,897,600
effective HSP length: 94
effective length of query: 415
effective length of database: 2,222,360
effective search space: 922279400
effective search space used: 922279400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 57 (26.6 bits)
Medicago: description of AC141109.8