BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC141106.8 - phase: 0
(337 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9510 similar to UP|O22709 (O22709) F8A5.23 protein, partial (49%) 71 3e-13
TC9667 similar to PIR|T52133|T52133 potassium channel beta subun... 59 1e-09
BP047508 55 1e-08
BP047468 54 3e-08
TC14770 similar to PIR|T52133|T52133 potassium channel beta subu... 52 1e-07
TC10388 similar to UP|Q84L20 (Q84L20) L-galactose dehydrogenase,... 51 3e-07
AV769441 46 1e-05
TC15665 similar to PIR|T02543|T02543 aldehyde dehydrogenase homo... 45 1e-05
TC15046 similar to UP|Q9FVN7 (Q9FVN7) NADPH-dependent mannose 6-... 41 3e-04
TC14344 similar to UP|Q40310 (Q40310) Chalcone reductase, complete 40 7e-04
TC8351 homologue to UP|Q41399 (Q41399) Chalcone reductase, complete 39 0.001
TC14346 similar to UP|Q40310 (Q40310) Chalcone reductase, partia... 38 0.003
BP037828 36 0.008
TC16005 weakly similar to UP|Q96425 (Q96425) Polyketide reductas... 35 0.023
CB829176 30 0.57
BE122451 29 1.3
TC10969 weakly similar to UP|O23148 (O23148) Patatin-like protei... 28 2.9
TC17688 similar to UP|Q94A68 (Q94A68) At1g06690/F4H5_17, partial... 28 2.9
BF177636 27 3.7
TC14525 weakly similar to UP|CHMT_MEDSA (P93324) Isoliquiritigen... 27 3.7
>TC9510 similar to UP|O22709 (O22709) F8A5.23 protein, partial (49%)
Length = 694
Score = 70.9 bits (172), Expect = 3e-13
Identities = 59/233 (25%), Positives = 99/233 (42%), Gaps = 4/233 (1%)
Frame = +3
Query: 51 VSRLCLGTM----TFGEQNTLSQSFQLLDEAYHAGINFFDSAEMYPVPQRAQTWGMSEEY 106
VS+L G M + + ++ A++ GI FFD+A++Y G +E
Sbjct: 81 VSKLGFGCMGLSGAYNDPVAEEDGVSIIKYAFNKGITFFDTADVYGAA------GANELL 242
Query: 107 FGHWIKHRNIPRDRLVIATKVAGPSGQMTWIRGGPKSLDATNISQAIDNSLLRMQLDYID 166
G +K + R+++ +ATK Q G + I+ SL R+ ++YID
Sbjct: 243 VGKALKQ--LHREKVQLATKFGISRTQSRDFSGMIVKGSPDYVRSCIEASLKRLDVEYID 416
Query: 167 LYQIHWPDRYVPMFGETEYDPVQQYSSISIDEQLDALSRAVNDGKIRYIGLSNETPYGLM 226
LY H D VP I++ + L + V + KI+YIGLS +P
Sbjct: 417 LYYQHRVDTSVP-----------------IEDTVGELKKLVEEEKIKYIGLSEASP---- 533
Query: 227 KFIQVAEKSSSYPKIVSLQNSYSLLCRTFDSAMAECCHQESISLLAYSPLAMG 279
++ + I ++Q +SL R + + C + I ++ YSPL G
Sbjct: 534 ---DTIRRAHAVHPITAVQIEWSLWTRDIEEEIVPLCRELGIGIVPYSPLGRG 683
>TC9667 similar to PIR|T52133|T52133 potassium channel beta subunit homolog
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (51%)
Length = 574
Score = 58.9 bits (141), Expect = 1e-09
Identities = 42/134 (31%), Positives = 65/134 (48%), Gaps = 3/134 (2%)
Frame = +2
Query: 49 LNVSRLCLGT-MTFGEQNTLSQSFQLLDEAYHAGINFFDSAEMYPVPQRAQTWGMSEEYF 107
L VS+L G ++FG Q + ++ LL G+NFFD+AE+Y G +EE
Sbjct: 101 LKVSQLSYGAWVSFGNQLDVKEAKSLLQCCRDHGVNFFDNAEVY-------ANGRAEEIM 259
Query: 108 GHWIKHRNIPRDRLVIATKVAGPSGQMTWIRGGP--KSLDATNISQAIDNSLLRMQLDYI 165
G I+ R +V++TK+ W GP K L +I + SL R+ ++Y+
Sbjct: 260 GQAIRELGWKRSDIVVSTKI-------FWGGQGPNDKGLSRKHIVEGTRASLKRLDMEYV 418
Query: 166 DLYQIHWPDRYVPM 179
D+ H PD P+
Sbjct: 419 DVIYCHRPDTSTPI 460
>BP047508
Length = 504
Score = 55.5 bits (132), Expect = 1e-08
Identities = 46/151 (30%), Positives = 73/151 (47%), Gaps = 5/151 (3%)
Frame = +3
Query: 77 AYHAGINFFDSAEMYPVPQRAQTWGMSEEYFGHWIKHRNIPRDRLVIATK-----VAGPS 131
A++ GI FFD++++Y ++E G +K ++PRD++ IATK V S
Sbjct: 138 AFNKGITFFDTSDVYGPH-------VNEILVGKALK--DLPRDQVQIATKFGIVKVEAGS 290
Query: 132 GQMTWIRGGPKSLDATNISQAIDNSLLRMQLDYIDLYQIHWPDRYVPMFGETEYDPVQQY 191
G + + G P+ + + SL R+ + YIDLY H D VP
Sbjct: 291 GNII-VNGTPEY-----VRSCCEASLQRLGVQYIDLYYQHRIDTTVP------------- 413
Query: 192 SSISIDEQLDALSRAVNDGKIRYIGLSNETP 222
I++ + L + V +GKI+YIGLS +P
Sbjct: 414 ----IEDTMGELKKLVQEGKIKYIGLSEASP 494
>BP047468
Length = 502
Score = 54.3 bits (129), Expect = 3e-08
Identities = 49/168 (29%), Positives = 77/168 (45%), Gaps = 4/168 (2%)
Frame = +2
Query: 55 CLG-TMTFGEQNTLSQSFQLLDEAYHAGINFFDSAEMYPVPQRAQTWGMSEEYFGHWIKH 113
C+G T + L+ A+ GI FFD++++Y ++E G +K
Sbjct: 38 CMGLTGVYNSPLPEEDGISLIKYAFSKGITFFDTSDVYGPH-------VNEVLVGKALK- 193
Query: 114 RNIPRDRLVIATK---VAGPSGQMTWIRGGPKSLDATNISQAIDNSLLRMQLDYIDLYQI 170
++PRD++ IATK V SG + + G P+ + + SL R+ + YIDLY
Sbjct: 194 -DLPRDQVQIATKFGIVKFDSGNVI-VNGTPEY-----VRSCCEASLQRLGVQYIDLYYQ 352
Query: 171 HWPDRYVPMFGETEYDPVQQYSSISIDEQLDALSRAVNDGKIRYIGLS 218
H D VP I++ + L + V +GKI+YIGLS
Sbjct: 353 HRIDTSVP-----------------IEDTMGELKKLVQEGKIKYIGLS 445
>TC14770 similar to PIR|T52133|T52133 potassium channel beta subunit homolog
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (75%)
Length = 1144
Score = 52.4 bits (124), Expect = 1e-07
Identities = 47/169 (27%), Positives = 74/169 (42%), Gaps = 3/169 (1%)
Frame = +2
Query: 134 MTWIRGGP--KSLDATNISQAIDNSLLRMQLDYIDLYQIHWPDRYVPMFGETEYDPVQQY 191
++W GP K L +I + SL R+ ++Y+D+ H PD P
Sbjct: 56 ISWGGQGPNDKGLSRKHIVEGTRASLKRLDMEYVDVIYCHRPDTSTP------------- 196
Query: 192 SSISIDEQLDALSRAVNDGKIRYIGLSNETPYGLMKFIQVAEKSSSYPKIVSLQNSYSLL 251
I+E + A++ ++ G Y G S + + + VA + IV Q Y+LL
Sbjct: 197 ----IEETVRAMNFVIDQGWAFYWGTSEWSAQQITEAWAVAHRLDLVGPIVE-QPEYNLL 361
Query: 252 CR-TFDSAMAECCHQESISLLAYSPLAMGILSGKYFSHGNGPADARLNL 299
R ++ I L +SPLA G+L+GKY G P+D+R L
Sbjct: 362 SRHKVEAEYLPLYSSYGIGLTTWSPLASGVLTGKY-KKGAIPSDSRFAL 505
>TC10388 similar to UP|Q84L20 (Q84L20) L-galactose dehydrogenase, partial
(44%)
Length = 466
Score = 50.8 bits (120), Expect = 3e-07
Identities = 44/153 (28%), Positives = 70/153 (44%), Gaps = 4/153 (2%)
Frame = +2
Query: 23 SQRSIEKVELMMSGGGGVPV----FNLAPNLNVSRLCLGTMTFGEQNTLSQSFQLLDEAY 78
+ + K+EL G G+ + F +P NV GT+ EQ S F A+
Sbjct: 17 TNKQTRKMELRELGRTGLKLSTVGFGASPLGNV----FGTVP-EEQAIASVRF-----AF 166
Query: 79 HAGINFFDSAEMYPVPQRAQTWGMSEEYFGHWIKHRNIPRDRLVIATKVAGPSGQMTWIR 138
+GINFFD++ Y +SE G +K N+PR ++ATK +
Sbjct: 167 QSGINFFDTSPFYGGT-------LSETVLGKALKALNVPRSDYIVATKCGRYKEGFDF-- 319
Query: 139 GGPKSLDATNISQAIDNSLLRMQLDYIDLYQIH 171
A ++++++ SL R+QLDY+D+ Q H
Sbjct: 320 ------SAERVTKSVEESLERLQLDYVDILQCH 400
>AV769441
Length = 594
Score = 45.8 bits (107), Expect = 1e-05
Identities = 30/85 (35%), Positives = 42/85 (49%)
Frame = +3
Query: 148 NISQAIDNSLLRMQLDYIDLYQIHWPDRYVPMFGETEYDPVQQYSSISIDEQLDALSRAV 207
++ +A D +L +QLDY+DLY IHWP V M Q + I A+
Sbjct: 324 DVPKAFDRTLRDLQLDYLDLYLIHWP---VSMKN-------GQLTKPDIPSTWRAMEALY 473
Query: 208 NDGKIRYIGLSNETPYGLMKFIQVA 232
N GK R IG+SN + L ++VA
Sbjct: 474 NSGKARAIGVSNFSVKKLQDLLEVA 548
>TC15665 similar to PIR|T02543|T02543 aldehyde dehydrogenase homolog
At2g37770 - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (59%)
Length = 582
Score = 45.4 bits (106), Expect = 1e-05
Identities = 28/81 (34%), Positives = 40/81 (48%)
Frame = +1
Query: 152 AIDNSLLRMQLDYIDLYQIHWPDRYVPMFGETEYDPVQQYSSISIDEQLDALSRAVNDGK 211
A D +L +QLDYIDLY IHWP R G + P + I A+ + GK
Sbjct: 340 AFDRTLQDLQLDYIDLYLIHWPIR--TKKGAVGFKP-ENLDQPDIPSTWRAMEALFDSGK 510
Query: 212 IRYIGLSNETPYGLMKFIQVA 232
+ IG+SN + L +++A
Sbjct: 511 AKAIGVSNFSSKKLQDLLEIA 573
>TC15046 similar to UP|Q9FVN7 (Q9FVN7) NADPH-dependent mannose 6-phosphate
reductase, partial (54%)
Length = 547
Score = 40.8 bits (94), Expect = 3e-04
Identities = 27/82 (32%), Positives = 47/82 (56%), Gaps = 7/82 (8%)
Frame = +1
Query: 145 DATNISQAIDNSLLRMQLDYIDLYQIHWP--DRYVPMFGET-----EYDPVQQYSSISID 197
D ++ +A +SL ++QLDY+DLY +H+P R+ + G T E + ++IS++
Sbjct: 289 DHGHVLEACKDSLKKLQLDYLDLYLVHFPVATRHTGV-GTTDSVKGEDGVLDIDTTISLE 465
Query: 198 EQLDALSRAVNDGKIRYIGLSN 219
A+ V+ G +R IG+SN
Sbjct: 466 TTWHAMEGLVSSGLVRSIGISN 531
>TC14344 similar to UP|Q40310 (Q40310) Chalcone reductase, complete
Length = 1180
Score = 39.7 bits (91), Expect = 7e-04
Identities = 38/150 (25%), Positives = 63/150 (41%), Gaps = 6/150 (4%)
Frame = +2
Query: 76 EAYHAGINFFDSAEMYPVPQRAQTWGMSEEYFGHWIKHRN----IPRDRLVIATKVAGPS 131
EA G FD+A Y SE+ G +K + R+ L + +K+
Sbjct: 206 EAIKQGYRHFDTAAAYG----------SEQALGEALKEALDLGLVSREELFVTSKL---- 343
Query: 132 GQMTWIRGGPKSLDATNISQAIDNSLLRMQLDYIDLYQIHWPDRYVPMFGETEYDPVQQY 191
W+ L + A+ SL +QL+Y+DLY IHWP P G+ + P+
Sbjct: 344 ----WVTENHPHL----VVPALQKSLKTLQLEYLDLYLIHWPLSSTP--GKFSF-PIAME 490
Query: 192 SSISIDEQ--LDALSRAVNDGKIRYIGLSN 219
+ D + +++ + G + IG+SN
Sbjct: 491 DLLPFDVKGVWESMEECLKLGLTKAIGVSN 580
>TC8351 homologue to UP|Q41399 (Q41399) Chalcone reductase, complete
Length = 1331
Score = 39.3 bits (90), Expect = 0.001
Identities = 34/143 (23%), Positives = 61/143 (41%), Gaps = 2/143 (1%)
Frame = +1
Query: 136 WIRGGPKSLDATN--ISQAIDNSLLRMQLDYIDLYQIHWPDRYVPMFGETEYDPVQQYSS 193
+I P + DA I A+ +L ++ ++Y+DLY IHWP R +
Sbjct: 277 FITSKPWNTDAHQDLIVPALKTTLKKLGVEYVDLYLIHWPVRLRHDLENPVVFSKEDLLP 456
Query: 194 ISIDEQLDALSRAVNDGKIRYIGLSNETPYGLMKFIQVAEKSSSYPKIVSLQNSYSLLCR 253
I+ A+ G + IG+ N +G K ++ E ++ P + ++ + S
Sbjct: 457 FDIEGTWKAMEECYKLGLAKSIGICN---FGTKKLTKLLEIATITPAVNQVEMNPSWQ-- 621
Query: 254 TFDSAMAECCHQESISLLAYSPL 276
+ E C Q+ I + A+S L
Sbjct: 622 --QGKLREFCKQKGIHVSAWSAL 684
>TC14346 similar to UP|Q40310 (Q40310) Chalcone reductase, partial (38%)
Length = 435
Score = 37.7 bits (86), Expect = 0.003
Identities = 29/102 (28%), Positives = 43/102 (41%), Gaps = 4/102 (3%)
Frame = +2
Query: 76 EAYHAGINFFDSAEMYPVPQRAQTWGMSEEYFGHWIKHRN----IPRDRLVIATKVAGPS 131
EA G FD+A Y SE+ G +K + R+ L + +K+
Sbjct: 191 EAIKQGYRHFDTAAAYG----------SEQALGEALKEALDLGLVSREELFVTSKL---- 328
Query: 132 GQMTWIRGGPKSLDATNISQAIDNSLLRMQLDYIDLYQIHWP 173
W+ L + A+ SL +QL+Y+DLY IHWP
Sbjct: 329 ----WVTENHPHL----VVPALQKSLKTLQLEYLDLYLIHWP 430
>BP037828
Length = 554
Score = 36.2 bits (82), Expect = 0.008
Identities = 14/23 (60%), Positives = 19/23 (81%)
Frame = -1
Query: 263 CHQESISLLAYSPLAMGILSGKY 285
C + IS++AYSP+A G+LSGKY
Sbjct: 545 CDELGISIIAYSPIAQGVLSGKY 477
>TC16005 weakly similar to UP|Q96425 (Q96425) Polyketide reductase (GGPKR1),
partial (40%)
Length = 474
Score = 34.7 bits (78), Expect = 0.023
Identities = 29/98 (29%), Positives = 44/98 (44%)
Frame = +1
Query: 76 EAYHAGINFFDSAEMYPVPQRAQTWGMSEEYFGHWIKHRNIPRDRLVIATKVAGPSGQMT 135
EA G FD+A Y V +++ ++E I R D L + +K+
Sbjct: 199 EAIKQGYRHFDAAAAYGV-EKSVGEAIAEALQLGLIASR----DELFVTSKL-------- 339
Query: 136 WIRGGPKSLDATNISQAIDNSLLRMQLDYIDLYQIHWP 173
W+ L I A+ SL +QL+Y+DL+ IHWP
Sbjct: 340 WVTDNHPHL----IVPALQKSLRTLQLEYLDLFLIHWP 441
>CB829176
Length = 536
Score = 30.0 bits (66), Expect = 0.57
Identities = 19/63 (30%), Positives = 29/63 (45%), Gaps = 2/63 (3%)
Frame = +2
Query: 267 SISLLAYSPLAMGILSGKYFSHGNGPADARLNLFKGLLTH--CNICFAFMCFHPFQHKRE 324
SI L + + ++ Y HG G D L+ F L C CF F F+PF+ +R
Sbjct: 338 SICFLHLNAFTLLVVVVIYCKHGGGGGDFLLDPFTFQLAEVIC*SCFNFFLFNPFRLQRV 517
Query: 325 KIL 327
++
Sbjct: 518 YVI 526
>BE122451
Length = 478
Score = 28.9 bits (63), Expect = 1.3
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = -1
Query: 285 YFSHGNGPADARLNLFKGLLTHCNICFAFMCFHPFQHKREKILNMFL 331
+FS N +++ NL + C IC F+ HPF H+ L FL
Sbjct: 268 FFSGRNLKRNSKTNLSLHCINQCLICLIFL--HPFLHELLTTLTPFL 134
>TC10969 weakly similar to UP|O23148 (O23148) Patatin-like protein
(Fragment), partial (45%)
Length = 765
Score = 27.7 bits (60), Expect = 2.9
Identities = 13/32 (40%), Positives = 21/32 (65%)
Frame = -1
Query: 224 GLMKFIQVAEKSSSYPKIVSLQNSYSLLCRTF 255
G+ F++V + K+VSL NS+++LCR F
Sbjct: 513 GITTFVKV------WLKVVSLNNSFTILCRYF 436
>TC17688 similar to UP|Q94A68 (Q94A68) At1g06690/F4H5_17, partial (31%)
Length = 553
Score = 27.7 bits (60), Expect = 2.9
Identities = 26/96 (27%), Positives = 44/96 (45%), Gaps = 15/96 (15%)
Frame = +3
Query: 48 NLNVSRLCLGTMTFGEQNTLSQSFQ-----------LLDEAYHAGINFFDSAEMYPVPQR 96
+L V+ L +G ++G+ T +F+ D + + G+ FFD+AE+Y
Sbjct: 231 DLKVTTLGIGAWSWGD-TTYWNNFEWNDRNEKAAKAAFDASINGGLTFFDTAEVY---GS 398
Query: 97 AQTWGM--SEEYFGHWIKHR--NIPRDRLVIATKVA 128
+G SE G +I+ R P + +ATK A
Sbjct: 399 GLAFGAVNSETLLGRFIRERKEKDPNVEVAVATKFA 506
>BF177636
Length = 483
Score = 27.3 bits (59), Expect = 3.7
Identities = 17/51 (33%), Positives = 24/51 (46%), Gaps = 2/51 (3%)
Frame = -2
Query: 268 ISLLAYSPLAMGILSG--KYFSHGNGPADARLNLFKGLLTHCNICFAFMCF 316
+S++ S L +G+L G + S GPA L FK L A+ CF
Sbjct: 407 VSVVPRSYLLLGVLDGTDRSSSPSGGPATGFLPSFKAFLISAAFHTAYFCF 255
>TC14525 weakly similar to UP|CHMT_MEDSA (P93324) Isoliquiritigenin
2'-O-methyltransferase (Chalcone O-methyltransferase)
(ChOMT) , partial (58%)
Length = 1077
Score = 27.3 bits (59), Expect = 3.7
Identities = 16/59 (27%), Positives = 28/59 (47%)
Frame = +3
Query: 220 ETPYGLMKFIQVAEKSSSYPKIVSLQNSYSLLCRTFDSAMAECCHQESISLLAYSPLAM 278
E P+ ++ Q + SY N+ +L+C+T +A +C SLL +P+ M
Sbjct: 165 EAPHKMVGSCQPLKSLLSYQP-----NTTTLICQTGSNACCDCSRATLFSLLPLAPVRM 326
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.138 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,323,641
Number of Sequences: 28460
Number of extensions: 86801
Number of successful extensions: 471
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 466
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 466
length of query: 337
length of database: 4,897,600
effective HSP length: 91
effective length of query: 246
effective length of database: 2,307,740
effective search space: 567704040
effective search space used: 567704040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 55 (25.8 bits)
Medicago: description of AC141106.8