BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139344.2 - phase: 0
(1591 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 227 2e-64
BP052632 102 6e-22
BP075943 79 6e-15
BP063694 65 1e-10
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 61 2e-09
AV427921 59 6e-09
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 56 4e-08
AU088980 55 7e-08
AV424544 48 1e-05
BP033602 48 1e-05
AV766665 44 2e-04
AV780009 44 3e-04
AV766088 43 3e-04
BP081717 38 0.011
BP085942 36 0.012
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 37 0.032
BP030995 36 0.055
AV773507 36 0.055
TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase regi... 35 0.12
BP078157 34 0.16
>BP066094
Length = 532
Score = 227 bits (579), Expect(3) = 2e-64
Identities = 109/149 (73%), Positives = 130/149 (87%)
Frame = +2
Query: 1293 ARLETIRLLLSYAINHGIILYQMDVKSVFLNGVIEEEVYVKQPPGFEDLKHPDHVYKLKK 1352
++ E IRLL+S+++NH IIL+QM+VKS FLNG I EEVYV QPPG ED K+ DH++KLKK
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 1353 SLYGLKQAPRAWYDRLSNFLIKNDFERGQVDTTLFRRTLKKDILIVQIYVDDIIFGSTNA 1412
SLYGLKQAPRAWY+RLS+FL++N+ RG+VDTTLF +T K DILIVQIYVDDIIFGS N
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 1413 SLCKEFSKLMQDEFEMSMMGELKFFLGIQ 1441
SLCKEFS++MQ EFEM MMGELK+FLGIQ
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 35.4 bits (80), Expect(3) = 2e-64
Identities = 16/18 (88%), Positives = 17/18 (93%)
Frame = +3
Query: 1277 YSQQEGINYTETFAPVAR 1294
YSQQ GI+YTETFAPVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
Score = 23.1 bits (48), Expect(3) = 2e-64
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +1
Query: 1267 RNKARLVAQG 1276
RNKARLVAQG
Sbjct: 10 RNKARLVAQG 39
>BP052632
Length = 489
Score = 102 bits (253), Expect = 6e-22
Identities = 48/75 (64%), Positives = 57/75 (76%)
Frame = -3
Query: 1011 KTAYELFKGRRPNISYFHQFGCTCYILNTKDYLKKFDAKAQRGIFLGYSERSKAYRVYNS 1070
KT YEL++GRRP +SYFH FGC +ILNT D + KFD K + I LGYS SKAY V+NS
Sbjct: 487 KTPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNS 308
Query: 1071 ETQCVEESMHVKFDD 1085
TQ VEES++VKFDD
Sbjct: 307 RTQVVEESINVKFDD 263
>BP075943
Length = 547
Score = 79.0 bits (193), Expect = 6e-15
Identities = 47/157 (29%), Positives = 78/157 (48%), Gaps = 1/157 (0%)
Frame = -3
Query: 1400 IYVDDIIFGSTNASLCKEFSKLMQDEFEMSMMGELKFFLGIQINQSKEGVYVHQTKYTKE 1459
+YVDD++ + + + D+F + +GE K+FLG++I +S G+ ++Q KY +
Sbjct: 515 LYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRKYALQ 336
Query: 1460 LLKKFKLEDCKVMNTPMHPTCTLSKEDTGT-VVDQKLYRGMIGSLLYLTASRPDILFSVC 1518
L+ + +TGT + D YR ++G LLYL +RPDI F+V
Sbjct: 335 LISDSGHFGFQPRFYSHGTNSQTLGTNTGTPLTDIGSYRRIVGRLLYLNTTRPDITFAVN 156
Query: 1519 LCARFQSDPRESHLTAVKRIFRYLKGTTNLGLLYRKS 1555
++F S P + H + +YL G+ GL Y S
Sbjct: 155 QLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPAS 45
>BP063694
Length = 511
Score = 64.7 bits (156), Expect = 1e-10
Identities = 40/116 (34%), Positives = 58/116 (49%), Gaps = 3/116 (2%)
Frame = -2
Query: 799 VWHKRLGHANWRLISKISK---LQLVKGLPNIDYHSDALCGACQKGKIVKSSFKSKDIVS 855
+WH RLGH N+ +I ++ K L + LP C +CQ K + F +
Sbjct: 360 LWHSRLGHVNFDIIKQLHKHGCLDVSSILPK-----PICCTSCQMAKSKRLVFHDNN-KR 199
Query: 856 TSRPLELLHIDLFGPVNTASLYGSKYGLVIVDDYSRWTWVKFIKSKDYACEVFSSF 911
S L+L+H DL GP AS+ G Y ++ VDD+SR+TW +K K +V F
Sbjct: 198 ASAVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRF 31
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 60.8 bits (146), Expect = 2e-09
Identities = 36/145 (24%), Positives = 74/145 (50%), Gaps = 9/145 (6%)
Frame = +2
Query: 1370 NFLIKNDFERGQVD-TTLFRRTLKKDILIVQIYVDDIIFGSTNASLCKEFSKLMQDEFEM 1428
+F++ + R D T +R D +I+ +YVDD++ N +E + EF+M
Sbjct: 8 SFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFDM 187
Query: 1429 SMMGELKFFLGIQINQSKEG--VYVHQTKYTKELLKKFKLEDCKVMNTPMHPTCTLSKED 1486
+G LG+QI++ ++ +++ Q Y +++L++F ++DC ++TP+ LS
Sbjct: 188 KDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSSM 367
Query: 1487 TGTVVDQKL------YRGMIGSLLY 1505
+ +++ Y +GSL+Y
Sbjct: 368 IPSSEAERMEMSRVPYASAVGSLMY 442
>AV427921
Length = 284
Score = 58.9 bits (141), Expect = 6e-09
Identities = 32/79 (40%), Positives = 43/79 (53%)
Frame = +1
Query: 957 TPQQNGVVERKNRTLQEMARTMIHENNLAKHFWAEAVNTSCYIQNRIYIRPMLEKTAYEL 1016
TPQQNGV ERKNRT+ M R+++ + L K F EAV S +I NR + E
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 1017 FKGRRPNISYFHQFGCTCY 1035
+ GR+P + +F C Y
Sbjct: 202 WSGRQPAVDHFRISRCLAY 258
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 56.2 bits (134), Expect = 4e-08
Identities = 34/99 (34%), Positives = 53/99 (53%), Gaps = 1/99 (1%)
Frame = -1
Query: 1453 QTKYTKELLKKFKLEDCKVMNTPM-HPTCTLSKEDTGTVVDQKLYRGMIGSLLYLTASRP 1511
Q Y ++LKKFK+ + K ++T + + + G VD Y+ +IGS+ YL R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 1512 DILFSVCLCARFQSDPRESHLTAVKRIFRYLKGTTNLGL 1550
DI+ V L +RF +P + H +R RY+KGT G+
Sbjct: 559 DIVCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGI 446
>AU088980
Length = 360
Score = 55.5 bits (132), Expect = 7e-08
Identities = 29/112 (25%), Positives = 52/112 (45%)
Frame = +2
Query: 961 NGVVERKNRTLQEMARTMIHENNLAKHFWAEAVNTSCYIQNRIYIRPMLEKTAYELFKGR 1020
N +VERK+R + + R ++ + L K+ W AV + Y+ N + + + Y+
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 1021 RPNISYFHQFGCTCYILNTKDYLKKFDAKAQRGIFLGYSERSKAYRVYNSET 1072
P + FG CY +K D +A++ +FLG+ + Y V + T
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGYIVMDLPT 337
>AV424544
Length = 276
Score = 48.1 bits (113), Expect = 1e-05
Identities = 23/90 (25%), Positives = 49/90 (53%)
Frame = +3
Query: 1367 RLSNFLIKNDFERGQVDTTLFRRTLKKDILIVQIYVDDIIFGSTNASLCKEFSKLMQDEF 1426
+LS++L + + D +LF + ++ +YVDD+I + + + + +F
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQF 185
Query: 1427 EMSMMGELKFFLGIQINQSKEGVYVHQTKY 1456
+ +G LK+FLG+++ +S G+++ Q KY
Sbjct: 186 RIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>BP033602
Length = 533
Score = 47.8 bits (112), Expect = 1e-05
Identities = 28/99 (28%), Positives = 53/99 (53%)
Frame = +3
Query: 792 SMNDKKWVWHKRLGHANWRLISKISKLQLVKGLPNIDYHSDALCGACQKGKIVKSSFKSK 851
S+ D+ +WH RLGH N++ + + L K N++ S C +C K ++ + S+
Sbjct: 255 SVRDQIMLWHNRLGHPNFQYLRHLFP-DLFK---NVNC-SSLECESCVLAKNQRAPYYSQ 419
Query: 852 DIVSTSRPLELLHIDLFGPVNTASLYGSKYGLVIVDDYS 890
SRP L+H D++GP + +G ++ + +DD++
Sbjct: 420 PY-HASRPFYLIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>AV766665
Length = 601
Score = 44.3 bits (103), Expect = 2e-04
Identities = 24/59 (40%), Positives = 32/59 (53%)
Frame = +3
Query: 1533 TAVKRIFRYLKGTTNLGLLYRKSLDYKLIGFCDADYAGDRIERKSTSGNCQFLGENLIS 1591
T RIF YLK + G L++K + GF ADY G ++R ST G FL NL++
Sbjct: 360 TFTTRIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVT 536
>AV780009
Length = 529
Score = 43.5 bits (101), Expect = 3e-04
Identities = 23/58 (39%), Positives = 32/58 (54%)
Frame = -1
Query: 1534 AVKRIFRYLKGTTNLGLLYRKSLDYKLIGFCDADYAGDRIERKSTSGNCQFLGENLIS 1591
A R+ RY+KG GL + KL + D+D+AG R+S +G FLG +LIS
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLIS 353
>AV766088
Length = 501
Score = 43.1 bits (100), Expect = 3e-04
Identities = 31/107 (28%), Positives = 57/107 (52%), Gaps = 4/107 (3%)
Frame = -3
Query: 799 VWHKRLGHANWRLISKISKLQLVKGLPNIDY---HSDALCGACQKGKIVKSSFKSKDIVS 855
+WH RLGH + S L+ ++ I Y + +C +C GK V+ F S + V
Sbjct: 307 LWHSRLGHPS------SSALRYLRSNKFISYELLNYSPVCESCVFGKHVRLPFVSSNNV- 149
Query: 856 TSRPLELLHIDLF-GPVNTASLYGSKYGLVIVDDYSRWTWVKFIKSK 901
T P ++LH DL+ PV +++ G ++ ++ +DD++ + W + +K
Sbjct: 148 TVMPFDILHSDLWTSPVLSSA--GHRFYVLFLDDFTDFLWTFPLSNK 14
>BP081717
Length = 291
Score = 38.1 bits (87), Expect = 0.011
Identities = 19/57 (33%), Positives = 28/57 (48%)
Frame = -1
Query: 799 VWHKRLGHANWRLISKISKLQLVKGLPNIDYHSDALCGACQKGKIVKSSFKSKDIVS 855
+WH RLGH + +++ ISK+ P + HS+ +C C K K F D S
Sbjct: 168 MWHFRLGHPSSKVLQHISKV-----FPYVKLHSNIICDFCHMAKQTKLPFPISDTKS 13
>BP085942
Length = 208
Score = 35.8 bits (81), Expect(2) = 0.012
Identities = 20/61 (32%), Positives = 32/61 (51%)
Frame = +3
Query: 879 SKYGLVIVDDYSRWTWVKFIKSKDYACEVFSSFCTQIQSEKELKILKVRSDHGGEFENEP 938
SK+ +V++D +I KD F++F TQ+Q E + ++ VRSDHG +
Sbjct: 6 SKHRVVLLDQ-------NWILHKDETHTRFTNFITQVQKEFQTSVITVRSDHGWRI*EQS 164
Query: 939 F 939
F
Sbjct: 165 F 167
Score = 21.2 bits (43), Expect(2) = 0.012
Identities = 9/21 (42%), Positives = 12/21 (56%)
Frame = +1
Query: 931 GGEFENEPFELFCEKHGILHE 951
GGEFEN+ E + G L +
Sbjct: 142 GGEFENKALEEWFNSQGNLSQ 204
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 36.6 bits (83), Expect = 0.032
Identities = 16/45 (35%), Positives = 29/45 (63%), Gaps = 8/45 (17%)
Frame = +2
Query: 294 KKFLSKRGSYKNS--KKEDQKG------CFNCKKPGHFIADCPDL 330
+K S R SY+++ +++ ++G C NCK+PGHF +CP++
Sbjct: 260 RKIRSDRFSYRDAPYRRDSRRGFSRDNLCKNCKRPGHFARECPNV 394
Score = 31.2 bits (69), Expect = 1.3
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +2
Query: 314 CFNCKKPGHFIADCPD 329
C+NCK+PGH + CP+
Sbjct: 458 CWNCKEPGHMASSCPN 505
>BP030995
Length = 458
Score = 35.8 bits (81), Expect = 0.055
Identities = 28/125 (22%), Positives = 50/125 (39%), Gaps = 28/125 (22%)
Frame = -3
Query: 721 INNVWLVDGLKHNLLSISQFC-DNEYDVTFSKTNCTLVNKDDKSITFKGKRVENV----- 774
+NN+ V G+ NL S+S FC N F +C + +++ + +G +
Sbjct: 420 LNNLLHVPGVCKNLSSVSXFCKQNNVFFEFHSDSCLVKSQETEETLLQGTLRNGLCAFDN 241
Query: 775 --YKINFSDLADQKVVCLLSMNDK--------------------KWVWHKRLGHANWRLI 812
+K+N + LLS + +WH RLGHA+ +++
Sbjct: 240 LLHKVNLHSASTLSNSALLSSSSPASALSVSVFSSNKCAQADIDSRLWHSRLGHASQKIV 61
Query: 813 SKISK 817
S + K
Sbjct: 60 SHVLK 46
>AV773507
Length = 496
Score = 35.8 bits (81), Expect = 0.055
Identities = 14/29 (48%), Positives = 16/29 (54%)
Frame = +2
Query: 300 RGSYKNSKKEDQKGCFNCKKPGHFIADCP 328
RGSY + Q CF C +PGH DCP
Sbjct: 308 RGSYGAGDRVGQDDCFECGRPGHRARDCP 394
>TC11573 similar to UP|Q9M6N4 (Q9M6N4) Pol protein integrase region
(Fragment), partial (10%)
Length = 572
Score = 34.7 bits (78), Expect = 0.12
Identities = 16/53 (30%), Positives = 30/53 (56%)
Frame = +2
Query: 929 DHGGEFENEPFELFCEKHGILHEFSSPRTPQQNGVVERKNRTLQEMARTMIHE 981
++G +F ++ + FC+ GI FSS + PQ NG E N+ + + + ++E
Sbjct: 8 ENGIQFTSKQTQDFCDGMGIQMRFSSVKHPQTNGQTEAANKVILKGIKRRLYE 166
>BP078157
Length = 395
Score = 34.3 bits (77), Expect = 0.16
Identities = 17/52 (32%), Positives = 27/52 (51%)
Frame = -2
Query: 799 VWHKRLGHANWRLISKISKLQLVKGLPNIDYHSDALCGACQKGKIVKSSFKS 850
+WH RLGH + +++ +S L LP+ H+ C C K+ + SF S
Sbjct: 148 LWHSRLGHLSDKVLKVVSNLVPFTVLPDFHSHN---CNVCPLSKMRRLSFVS 2
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.131 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,350,575
Number of Sequences: 28460
Number of extensions: 311949
Number of successful extensions: 1450
Number of sequences better than 10.0: 74
Number of HSP's better than 10.0 without gapping: 1416
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1441
length of query: 1591
length of database: 4,897,600
effective HSP length: 102
effective length of query: 1489
effective length of database: 1,994,680
effective search space: 2970078520
effective search space used: 2970078520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 62 (28.5 bits)
Medicago: description of AC139344.2