BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137077.1 - phase: 0
(1555 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9543 164 1e-40
TC13324 149 4e-36
TC18445 64 2e-10
TC9938 weakly similar to GB|AAM97131.1|22531255|AY136466 ribonuc... 42 0.001
TC19911 weakly similar to PIR|A44805|A44805 eggshell protein pre... 39 0.005
TC10062 similar to BAD08700 (BAD08700) Cold shock domain protein... 37 0.024
TC7812 UP|Q9LKJ6 (Q9LKJ6) Water-selective transport intrinsic me... 36 0.053
CN825770 35 0.070
TC14274 weakly similar to GB|AAF79559.1|8778551|AC022464 F22G5.3... 35 0.070
TC10403 similar to UP|O80815 (O80815) T8F5.22 protein, partial (... 35 0.091
BP070010 35 0.091
TC8088 similar to UP|Q9C5Q6 (Q9C5Q6) Fasciclin-like arabinogalac... 35 0.091
TC14338 similar to UP|Q9MAH5 (Q9MAH5) F12M16.16, partial (33%) 35 0.12
TC16174 similar to UP|Q940N7 (Q940N7) AT4g05150/C17L7_70, partia... 34 0.20
BP033767 34 0.20
TC13393 similar to UP|Q9FMU6 (Q9FMU6) Mitochondrial phosphate tr... 33 0.27
TC15523 similar to UP|GRPA_MAIZE (P10979) Glycine-rich RNA-bindi... 33 0.35
TC9507 similar to UP|Q8VZB6 (Q8VZB6) DnaJ-like protein, partial ... 33 0.45
CB826639 33 0.45
TC7814 similar to UP|Q9LKJ6 (Q9LKJ6) Water-selective transport i... 31 1.3
>TC9543
Length = 1003
Score = 164 bits (414), Expect = 1e-40
Identities = 77/113 (68%), Positives = 89/113 (78%)
Frame = +3
Query: 1443 ANENPSTSSTNDWSYHPPKHAQTMTMQRFPSAGNISKQGQTDGNESHFSHSRRTASWSGS 1502
ANE+PSTSSTNDWSY PPKH + MQ+FPS GNISKQG T G+ SHF++SRRTASWSG+
Sbjct: 3 ANEDPSTSSTNDWSYQPPKHVSAVAMQKFPSMGNISKQGATGGSNSHFANSRRTASWSGN 182
Query: 1503 FNDSFSPPKMGEIKPSGAALGMPPSAFMPDPSSLMQGPTRSGSFGEDLQEVEL 1555
ND+FSPP + E KP G ALGMPP F+P+ SS M +S SFGEDLQEVEL
Sbjct: 183 VNDAFSPPTVRETKPLGDALGMPPLTFIPNQSSSMHPTMKSSSFGEDLQEVEL 341
>TC13324
Length = 430
Score = 149 bits (375), Expect = 4e-36
Identities = 87/161 (54%), Positives = 95/161 (58%), Gaps = 6/161 (3%)
Frame = +2
Query: 533 GSWSGSHGVNHQQGFDMYATEASTKIGNNTASSGNQQVHHSYGNQ------QVNTSSSFG 586
GSWSG GVNHQQG DMY E + K + S QQV+HSYG+ Q NTSSSFG
Sbjct: 2 GSWSGLQGVNHQQGIDMYTAETTAKREDYITSGIYQQVNHSYGSSISETKDQQNTSSSFG 181
Query: 587 SVALNNKGSFEPKAFVPHRDIAHQFNYQDTEFDNGTFAPKTFVPHGDIAQQFNYPNTKFD 646
SVAL NK V H D F NGTF PK+FVP G+I QQFNY +TKFD
Sbjct: 182 SVALYNK--------VNH----------DHGFANGTFEPKSFVPSGNIGQQFNYSSTKFD 307
Query: 647 EQKQFSNVFAENQNSHSYSQQPIQGGLQYSYAPHAGRSSAG 687
EQK+FSN FA NQ SYS Q G YAP GRSSAG
Sbjct: 308 EQKKFSNEFAGNQKPFSYSYQSSPDGYHSQYAPDVGRSSAG 430
>TC18445
Length = 532
Score = 63.9 bits (154), Expect = 2e-10
Identities = 32/49 (65%), Positives = 35/49 (71%)
Frame = +1
Query: 1507 FSPPKMGEIKPSGAALGMPPSAFMPDPSSLMQGPTRSGSFGEDLQEVEL 1555
+ P M E KP G ALGMPPS FMPD +SLM P +SGSFG DL EVEL
Sbjct: 4 YPTPHMNEAKPLGEALGMPPSTFMPDDASLMHTPVKSGSFG-DLHEVEL 147
>TC9938 weakly similar to GB|AAM97131.1|22531255|AY136466
ribonucleoprotein-like {Arabidopsis thaliana;}, partial
(9%)
Length = 633
Score = 41.6 bits (96), Expect = 0.001
Identities = 32/107 (29%), Positives = 44/107 (40%)
Frame = +1
Query: 434 YSQAGNHVSQGVGSQAVNGSWSGSHGVSQAGNYDGSHGVGSQAVNGSWSGSHGVNQAGNY 493
Y G+ G G + G + AG Y GS G G + G+ A
Sbjct: 34 YGGYGSEFGGYGGYAGAMGPYRGDPSLGFAGRYGGSFSRGYDL--GGYGGASESYGAYGA 207
Query: 494 GGSQGVGSQAVNGSWSGSHGVNQAGNYGGSQGVGSQAVNGSWSGSHG 540
G + G GS A G++ G + + AG YGG+ G GS+ GS G
Sbjct: 208 GAAAGGGSSAA-GAYQGGYDASLAGGYGGASG-------GSFYGSRG 324
>TC19911 weakly similar to PIR|A44805|A44805 eggshell protein precursor -
fluke (Schistosoma haematobium) {Schistosoma
haematobium;} , partial (24%)
Length = 350
Score = 39.3 bits (90), Expect = 0.005
Identities = 33/111 (29%), Positives = 44/111 (38%), Gaps = 5/111 (4%)
Frame = +1
Query: 434 YSQAGNHVSQGVGSQAVNG---SWSGSHGVSQAGNYDGSHGVGSQAVNGSWSGSHGVNQA 490
+S G+H + S +G S + HG+S G YD + G + G G H
Sbjct: 37 HSGGGSHGNDHRHSDNEHGGRRSETTGHGLS-GGGYDDDNRSGGRTGGGYGDGGHSAGGY 213
Query: 491 GNYGGSQGVGSQAV--NGSWSGSHGVNQAGNYGGSQGVGSQAVNGSWSGSH 539
G++GG G G G SGS G G G G +G SG H
Sbjct: 214 GDHGGRSGGGGGGYGDGGLSSGSGGYGDGGRSGVGYG------DGXLSGGH 348
>TC10062 similar to BAD08700 (BAD08700) Cold shock domain protein 2, partial
(66%)
Length = 481
Score = 37.0 bits (84), Expect = 0.024
Identities = 29/97 (29%), Positives = 32/97 (32%)
Frame = +3
Query: 444 GVGSQAVNGSWSGSHGVSQAGNYDGSHGVGSQAVNGSWSGSHGVNQAGNYGGSQGVGSQA 503
G QAV G+ G G G G +G G G G G G YGG G G
Sbjct: 243 GPNGQAVQGTRRGG-GAGGGGRGGGGYGGGGYGGGGYGGGGRG---GGGYGGGGGYGG-- 404
Query: 504 VNGSWSGSHGVNQAGNYGGSQGVGSQAVNGSWSGSHG 540
+G YGG G G G G G
Sbjct: 405 -------------SGGYGGGGGYGGGGRGGGRGGGGG 476
>TC7812 UP|Q9LKJ6 (Q9LKJ6) Water-selective transport intrinsic membrane
protein 1, complete
Length = 1149
Score = 35.8 bits (81), Expect = 0.053
Identities = 33/108 (30%), Positives = 44/108 (40%), Gaps = 12/108 (11%)
Frame = -2
Query: 41 DEGNDSDDANAFANLSISDVDAGAFDNSVVGESGVEVKEELGVVKSDVGLDG-------- 92
DEG + + LS DVD A + V + E + V DVG DG
Sbjct: 446 DEGEEQGGDDGSEKLS-DDVDDTAEEGDVTADEEAEGDGGVDVAAGDVGADGDGDEEGEC 270
Query: 93 ----GGDDDGKEGNLLMGSSSVECDSKTELGKEEIGIGSEFTAVAPVG 136
GGD+ G G ++G V DS T G++E G E + P G
Sbjct: 269 VGDGGGDEAGGGGGAVVGEFVVG-DSGTGAGEDEDEGGDELSQG*PQG 129
>CN825770
Length = 586
Score = 35.4 bits (80), Expect = 0.070
Identities = 31/95 (32%), Positives = 39/95 (40%)
Frame = +3
Query: 446 GSQAVNGSWSGSHGVSQAGNYDGSHGVGSQAVNGSWSGSHGVNQAGNYGGSQGVGSQAVN 505
G+ V G+ G G + N G G Q GS GS+ + G+ G+ G G N
Sbjct: 3 GNPGVYGAVGGRTGSTPNSNASGPGGSELQGSGGS--GSYMGSGYGDANGNSGYG----N 164
Query: 506 GSWSGSHGVNQAGNYGGSQGVGSQAVNGSWSGSHG 540
+W S GNYG QG NGS G G
Sbjct: 165 AAWR-SEQAQATGNYGTPQG------NGSHGGQVG 248
>TC14274 weakly similar to GB|AAF79559.1|8778551|AC022464 F22G5.35
{Arabidopsis thaliana;} , partial (16%)
Length = 1208
Score = 35.4 bits (80), Expect = 0.070
Identities = 40/116 (34%), Positives = 52/116 (44%), Gaps = 4/116 (3%)
Frame = +3
Query: 1313 ALPPPPPTTAAFQNGSADYNLKSALKTEGLTPNEFSSTRTSSPELSPGMPPIPPSSNQFS 1372
A+ PP PTT A S S T LTP FS +R+S+P PPIPP+ +
Sbjct: 174 AVSPPNPTTPATPPPSGTSVSPSLSLTPSLTP--FSPSRSSTPN-----PPIPPNPSSPP 332
Query: 1373 ARSRLGVRSRYVDTFNQNGGSSANLFQSPSVQSVKPALP----ANAKFFIPAPVPS 1424
+ S S T + +SAN SPS S PA P ++ + AP PS
Sbjct: 333 SAS-----SSPTSTTSMIPPASAN---SPS--SAPPAAPTARSTSSSASLVAPNPS 470
>TC10403 similar to UP|O80815 (O80815) T8F5.22 protein, partial (15%)
Length = 1392
Score = 35.0 bits (79), Expect = 0.091
Identities = 33/116 (28%), Positives = 42/116 (35%), Gaps = 1/116 (0%)
Frame = +3
Query: 452 GSWSGSHGV-SQAGNYDGSHGVGSQAVNGSWSGSHGVNQAGNYGGSQGVGSQAVNGSWSG 510
GS+S S G S D +G G S G+ G++ G +A G W G
Sbjct: 846 GSYSSSRGHNSNNERQDSGYGAGRWGP-ASKDGNDGLSNFPGAKVQNSPGREAFPGGWGG 1022
Query: 511 SHGVNQAGNYGGSQGVGSQAVNGSWSGSHGVNHQQGFDMYATEASTKIGNNTASSG 566
S G +GG G NG W G G A E S+ G T +G
Sbjct: 1023SDG---GSGWGGGASTGD---NGGWGQGSG-----GDQNDAQEGSSGWGTGTKKAG 1157
>BP070010
Length = 519
Score = 35.0 bits (79), Expect = 0.091
Identities = 35/102 (34%), Positives = 42/102 (40%), Gaps = 2/102 (1%)
Frame = +2
Query: 1312 AALPPPPPTTAAFQNGSADYNLKSALKTEGLTPNEFSSTR-TSSPELSPGMPPIPPSSNQ 1370
AA PPPP A F N +A + SA L P SS SP + PP SS+
Sbjct: 200 AATPPPPTAAAFFANSAASFPSPSATSV-ALFPQSCSSKP*LGSPPATITSPP-SSSSSS 373
Query: 1371 FSARSRLGVRSRYVDTFNQNGGSSANLFQ-SPSVQSVKPALP 1411
S L + R SS +L Q SPS PA+P
Sbjct: 374 SSTTPPLSIAMRLSSFNTICKSSSLSLSQSSPSPPPPPPAVP 499
>TC8088 similar to UP|Q9C5Q6 (Q9C5Q6) Fasciclin-like arabinogalactan-protein
2, partial (69%)
Length = 1527
Score = 35.0 bits (79), Expect = 0.091
Identities = 42/154 (27%), Positives = 67/154 (43%), Gaps = 10/154 (6%)
Frame = +2
Query: 1315 PPPPPTTAAFQNGSADYNLKSALKTEGLTPNEFSSTRTSSPELSPG----MPPIPPSSNQ 1370
P PPP AA + N ++ + +P+ S R+++ + SP PP PPSS
Sbjct: 119 PQPPPPMAATTLPAFSPNTPTSPPSTTTSPSPTSPARSTTAKPSPSSPSTTPPCPPSSTS 298
Query: 1371 FSARSRLGVRSRYVDTFNQNGGSSANLFQSPSVQ--SVKPALPANAKFFIPAPVP---SS 1425
S S T + + SS L S + + P+ P ++ P P P S+
Sbjct: 299 TSP-------SPP*KTSSPSTSSSTTLAPKSSTR*PTEPPSSPPCSRPPAPPPEPPATST 457
Query: 1426 SEQNMEAIAESNLEDSAANENPSTSSTNDW-SYH 1458
S + EA S L + A+ + S++N W S+H
Sbjct: 458 SPTSKEAK*ASPLRTTTASTH---STSNQWKSFH 550
>TC14338 similar to UP|Q9MAH5 (Q9MAH5) F12M16.16, partial (33%)
Length = 1751
Score = 34.7 bits (78), Expect = 0.12
Identities = 37/142 (26%), Positives = 49/142 (34%), Gaps = 25/142 (17%)
Frame = +2
Query: 446 GSQAVNGSWSGSHGVSQAGNYDGSHGVGSQAVNGSWSGSHGVNQAG-------------- 491
G N G H +QAG Y + G N G +QAG
Sbjct: 929 GGYPPNNQAGGYHPQNQAGGYPPNSQAGGYPPNSQAGGYPPNSQAGVYPPNNQGVYPPNQ 1108
Query: 492 -NYGG--SQGVGSQAVNGSWSGSHGV-----NQAGNYGG---SQGVGSQAVNGSWSGSHG 540
NY G VG N ++ + G N AGN G +Q VG + N WS
Sbjct: 1109 NNYAGRTPPNVGGAPPNSAYGPNAGYPSNQNNYAGNMEGVPPNQNVGGYSPNAGWS---- 1276
Query: 541 VNHQQGFDMYATEASTKIGNNT 562
N+ D + + +G NT
Sbjct: 1277 -NNAPTRDTPSRDFGAAVGGNT 1339
>TC16174 similar to UP|Q940N7 (Q940N7) AT4g05150/C17L7_70, partial (20%)
Length = 491
Score = 33.9 bits (76), Expect = 0.20
Identities = 20/57 (35%), Positives = 32/57 (56%), Gaps = 2/57 (3%)
Frame = +2
Query: 1315 PPPPPTTAAFQNGSADYNLKSALKTEGLTPNEFSSTRTSSPELSPG--MPPIPPSSN 1369
PP PT+ ++ SA ++ +A + T S+T ++PE SP +PP PPSS+
Sbjct: 173 PPLAPTSMTRRHASASWSASAARSSPAPTTIS-SATSAATPESSPSTALPPSPPSSS 340
>BP033767
Length = 541
Score = 33.9 bits (76), Expect = 0.20
Identities = 23/69 (33%), Positives = 31/69 (44%)
Frame = +1
Query: 1163 LPPPAPTSSQATVHGSEQHYQHMAPRVSTSQSTMAMSSLVPSASLEPISEWTADNNRMAA 1222
LPPP+P S + S + P S+S S + S +SL P S TAD
Sbjct: 328 LPPPSPPSPPTSPPSSSLKPPNPNPPTSSSASPLPPSPAPSPSSLSPPSS-TADAAAKTT 504
Query: 1223 KPNRSVSEP 1231
P R +S+P
Sbjct: 505 PPTRRLSDP 531
>TC13393 similar to UP|Q9FMU6 (Q9FMU6) Mitochondrial phosphate translocator
(AT5g14040/MUA22_4), partial (8%)
Length = 541
Score = 33.5 bits (75), Expect = 0.27
Identities = 40/147 (27%), Positives = 58/147 (39%), Gaps = 2/147 (1%)
Frame = +1
Query: 1350 TRTSSPELSPGMPPIPPSSNQFSARSRLGVRSRYVDTFNQNGGSSANLFQSPSVQSVKPA 1409
TRTSSP SP P PPS+ S G +A P+ S + +
Sbjct: 97 TRTSSPSSSPHSVP-PPSTPSKSF------------PITPTGSLAA-----PAASSSRTS 222
Query: 1410 LPANAKFFIPAPVPSSSEQNMEAIAESNLEDSAANENPSTSSTNDWSYHPPKHAQTMTMQ 1469
P+ A P P SSS A ++ + S +P+T ST S +T+
Sbjct: 223 PPSPAPSLSPNPRSSSSSPTSSASPKTPMISSPGLRSPNTDSTTPSSMQ--ASPSDLTIL 396
Query: 1470 RFPSAGNISKQG--QTDGNESHFSHSR 1494
F + G + + G Q GN + S S+
Sbjct: 397 LFSTPGMVFRGGSCQNGGNLNSGSGSK 477
>TC15523 similar to UP|GRPA_MAIZE (P10979) Glycine-rich RNA-binding,
abscisic acid-inducible protein, complete
Length = 972
Score = 33.1 bits (74), Expect = 0.35
Identities = 26/78 (33%), Positives = 33/78 (41%), Gaps = 6/78 (7%)
Frame = +2
Query: 464 GNYDGSHGVGSQAVNGSWSGSHGVNQAGNYGGSQGVGSQAVNGSWSGSHGVNQ---AGNY 520
G G G G G + G G +G GG G GS G +G + G Y
Sbjct: 602 GGGGGGGGYGGGRREGGYGGGGGSRYSGGGGGGYG-------GSGGGGYGGRREGGGGGY 760
Query: 521 GGSQ--GVGSQ-AVNGSW 535
GGS+ G GS+ + GSW
Sbjct: 761 GGSREGGGGSRYSSGGSW 814
Score = 32.3 bits (72), Expect = 0.59
Identities = 25/86 (29%), Positives = 32/86 (37%), Gaps = 3/86 (3%)
Frame = +2
Query: 426 DDNSLYSEYSQAGNHVSQGVGSQAVNGSWSGSHGVSQAGNYDGSHGVGSQAVNGSWSGSH 485
D ++ +QA G G G G +G Y G G G G G
Sbjct: 557 DGRNVTVNEAQARGRGGGGGGGGYGGGRREGGYGGGGGSRYSGGGGGGYGGSGGGGYGGR 736
Query: 486 GVNQAGNYGGSQ--GVGSQ-AVNGSW 508
G YGGS+ G GS+ + GSW
Sbjct: 737 REGGGGGYGGSREGGGGSRYSSGGSW 814
Score = 30.8 bits (68), Expect = 1.7
Identities = 26/95 (27%), Positives = 37/95 (38%), Gaps = 9/95 (9%)
Frame = +2
Query: 461 SQAGNYDGSHGVGSQAVNGSWSGSHGVNQA-----GNYGGSQGVGSQAVNGSWSGSHGVN 515
S+ D G+ Q ++G + VN+A G GG G G G + G G
Sbjct: 506 SEQSMKDAIEGMNGQDIDGR---NVTVNEAQARGRGGGGGGGGYGGGRREGGYGGGGGSR 676
Query: 516 QAGN----YGGSQGVGSQAVNGSWSGSHGVNHQQG 546
+G YGGS G G G +G + + G
Sbjct: 677 YSGGGGGGYGGSGGGGYGGRREGGGGGYGGSREGG 781
>TC9507 similar to UP|Q8VZB6 (Q8VZB6) DnaJ-like protein, partial (40%)
Length = 905
Score = 32.7 bits (73), Expect = 0.45
Identities = 20/56 (35%), Positives = 29/56 (51%)
Frame = -1
Query: 1327 GSADYNLKSALKTEGLTPNEFSSTRTSSPELSPGMPPIPPSSNQFSARSRLGVRSR 1382
G+A + +TEGL+ F + +S LSP PP PP + S+ R+G R R
Sbjct: 431 GAAAAEAEDLRRTEGLSAAGFPGSAAASVGLSPAPPP-PPIAVNRSSTGRVGSRRR 267
>CB826639
Length = 542
Score = 32.7 bits (73), Expect = 0.45
Identities = 22/66 (33%), Positives = 27/66 (40%)
Frame = -3
Query: 1315 PPPPPTTAAFQNGSADYNLKSALKTEGLTPNEFSSTRTSSPELSPGMPPIPPSSNQFSAR 1374
PPPPP T+ F + L LTP FS R L+ PP++ S R
Sbjct: 363 PPPPPPTSPFPR-------RECLSKPPLTPRRFSPPRAGGSSLARKTSSAPPTTCP-SRR 208
Query: 1375 SRLGVR 1380
R VR
Sbjct: 207 GRSSVR 190
>TC7814 similar to UP|Q9LKJ6 (Q9LKJ6) Water-selective transport intrinsic
membrane protein 1, partial (37%)
Length = 660
Score = 31.2 bits (69), Expect = 1.3
Identities = 24/77 (31%), Positives = 35/77 (45%), Gaps = 6/77 (7%)
Frame = -1
Query: 66 DNSVVGESGVEVK------EELGVVKSDVGLDGGGDDDGKEGNLLMGSSSVECDSKTELG 119
D G+ GV+V + G + + DGGGD+ G G ++G V DS T G
Sbjct: 660 DEEXEGDXGVDVAAGDVGDDXDGDEEGECMGDGGGDEXGGGGGTVIGEFIVG-DSGTGAG 484
Query: 120 KEEIGIGSEFTAVAPVG 136
++E G E + P G
Sbjct: 483 EDEDEGGDELSXGXPQG 433
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.310 0.128 0.370
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,278,525
Number of Sequences: 28460
Number of extensions: 362101
Number of successful extensions: 2618
Number of sequences better than 10.0: 101
Number of HSP's better than 10.0 without gapping: 2263
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2556
length of query: 1555
length of database: 4,897,600
effective HSP length: 102
effective length of query: 1453
effective length of database: 1,994,680
effective search space: 2898270040
effective search space used: 2898270040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 62 (28.5 bits)
Medicago: description of AC137077.1