BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC134823.6 + phase: 0
(662 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9445 similar to PIR|T08956|T08956 AIM1 protein - Arabidopsis t... 363 0.0
BI418281 271 2e-73
AV407350 148 2e-36
TC13883 weakly similar to UP|Q9LDF5 (Q9LDF5) 3-hydroxybutyryl-Co... 90 1e-18
AV780782 66 2e-11
TC15752 similar to PIR|T08956|T08956 AIM1 protein - Arabidopsis ... 51 7e-07
TC14643 similar to UP|Q9T0K7 (Q9T0K7) 3-hydroxyisobutyryl-coenzy... 48 6e-06
TC16259 weakly similar to UP|O23300 (O23300) Carnitine racemase ... 44 6e-05
TC11677 weakly similar to UP|Q87R67 (Q87R67) Naphthoate synthase... 40 0.001
BP073016 40 0.001
TC10228 similar to UP|Q9LDF5 (Q9LDF5) 3-hydroxybutyryl-CoA dehyd... 35 0.050
AV421087 35 0.050
AV410093 30 0.95
TC14665 29 2.1
TC13714 homologue to UP|Q7XA53 (Q7XA53) Sucrose transporter, par... 28 6.2
TC9835 similar to AAQ56831 (AAQ56831) At3g03960, partial (67%) 27 8.0
TC19171 similar to UP|Q9SXV6 (Q9SXV6) Cycloartenol synthase, par... 27 8.0
>TC9445 similar to PIR|T08956|T08956 AIM1 protein - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (58%)
Length = 1401
Score = 363 bits (932), Expect(2) = 0.0
Identities = 176/206 (85%), Positives = 193/206 (93%)
Frame = +3
Query: 457 VRTNKTSAQVILDLVTVGKIIKKSPVVVGNCTGFAVNRTFFPYAQGAHFLANLGVDVFRI 516
VRT+KTS+Q ILDL+TVGKIIKKSPVVVGNCTGFAVNR FFPY+QGAH L NLGVDVFRI
Sbjct: 555 VRTDKTSSQAILDLMTVGKIIKKSPVVVGNCTGFAVNRAFFPYSQGAHLLVNLGVDVFRI 734
Query: 517 DRLISNFGLPMGPFQLQDLSGYGVAVAVGKEFAGSFAGRTFPTPLLDLLIKSGRNGKNNG 576
D+LISNFGLPMGPFQLQDL+GYGVA+AVGKEF +F RTF T LL+LLIKSGRNGKNNG
Sbjct: 735 DKLISNFGLPMGPFQLQDLAGYGVAMAVGKEFTTAFPDRTFRTTLLELLIKSGRNGKNNG 914
Query: 577 KGYYIYEKGSKPKPDPSVLPIVEESRRLSNIMPNGKPISITDQEIVEMILFPVVNEACRV 636
KGYY+YEKGSKPKPD S+LPI+EESRRL NIMP GKPIS+TDQEIVEMILFP+VNE+CR+
Sbjct: 915 KGYYVYEKGSKPKPDASILPIIEESRRLCNIMPGGKPISVTDQEIVEMILFPIVNESCRI 1094
Query: 637 LEEGIVIRASDLDIASVLGMSFPSYR 662
LEEG+VIRASDLDIASVLGMSFPSYR
Sbjct: 1095LEEGVVIRASDLDIASVLGMSFPSYR 1172
Score = 306 bits (785), Expect(2) = 0.0
Identities = 155/180 (86%), Positives = 166/180 (92%)
Frame = +2
Query: 277 AKGLIHVFFAQRTISKIPGVTDIGLKPRNVRKAAVIGGGLMGSGIATALILGNIRVILKE 336
AKGLIHVFFAQR ISK+PGVTDIGLKPRNVRKAAVIGGGLMGSGIATALILGNI VILKE
Sbjct: 14 AKGLIHVFFAQRAISKVPGVTDIGLKPRNVRKAAVIGGGLMGSGIATALILGNIHVILKE 193
Query: 337 VNSEYLQKGIKTIEANVRGLVTRKKLTQQKAEGALSLLKGVLDYAEFKDVDMVIEAVIEK 396
VNSEYL KGIK IEANV GLV KLT+QKA+GALSLLKGVLDY E KDVD+VIEAVIE
Sbjct: 194 VNSEYLHKGIKFIEANVSGLVRTGKLTKQKADGALSLLKGVLDYTECKDVDLVIEAVIEN 373
Query: 397 VSLKQDIFSDLEKICPPHCILASNTSTIDLNIIGEKISSQDRVIGAHFFSPAHIMPLLEI 456
V LKQ IFSDLEKICPPHCIL++NTSTIDLN++G+K SSQDR++GAHFFSPAHIMPLLEI
Sbjct: 374 VGLKQTIFSDLEKICPPHCILSTNTSTIDLNLVGQKTSSQDRIVGAHFFSPAHIMPLLEI 553
>BI418281
Length = 494
Score = 271 bits (694), Expect = 2e-73
Identities = 135/147 (91%), Positives = 144/147 (97%)
Frame = +2
Query: 1 MASVKVDFEVGNDGVAVITMCNPPVNALAIPIIRGLKNKFEEAARRNDVKAIVLTGKGGR 60
MASVKVDFEVGNDGVAVITMCNPPVNALA+PII+ LK++F+EAARRNDVKAIVLTGKGGR
Sbjct: 53 MASVKVDFEVGNDGVAVITMCNPPVNALAVPIIKALKDRFDEAARRNDVKAIVLTGKGGR 232
Query: 61 FSGGFDISVMQKVHQTGDITLVPDVSVELVVNSIEDSKKPVVAAVEGLALGGGLELAMGC 120
FSGGFDISVMQKVHQTGD+TLVPDVSVELVVN+IEDSKKPVVAAVEGLALGGGL+LAMGC
Sbjct: 233 FSGGFDISVMQKVHQTGDVTLVPDVSVELVVNAIEDSKKPVVAAVEGLALGGGLKLAMGC 412
Query: 121 HARVAAPKAQLGLPELTLGIIPGFGGT 147
HARVAAP+ LGLPELTLGIIPGFGGT
Sbjct: 413 HARVAAPRT*LGLPELTLGIIPGFGGT 493
>AV407350
Length = 425
Score = 148 bits (374), Expect = 2e-36
Identities = 69/133 (51%), Positives = 94/133 (69%)
Frame = +1
Query: 473 VGKIIKKSPVVVGNCTGFAVNRTFFPYAQGAHFLANLGVDVFRIDRLISNFGLPMGPFQL 532
+ K IKK+PVVVGNCTGFAVNR FFPY Q A FL G DV++ID++I+ FG+PMGPF+L
Sbjct: 7 ISKKIKKTPVVVGNCTGFAVNRIFFPYTQAALFLVERGADVYQIDKVITKFGMPMGPFRL 186
Query: 533 QDLSGYGVAVAVGKEFAGSFAGRTFPTPLLDLLIKSGRNGKNNGKGYYIYEKGSKPKPDP 592
DL G+GVA+A G +F +F RT+ + L+ LL + R G+ KG+Y+Y+ KP PDP
Sbjct: 187 CDLVGFGVAIATGTQFVQNFPERTYKSMLIPLLQEDKRAGETTRKGFYLYDDKRKPSPDP 366
Query: 593 SVLPIVEESRRLS 605
+ +E++R S
Sbjct: 367 ELKNYIEKARSTS 405
>TC13883 weakly similar to UP|Q9LDF5 (Q9LDF5) 3-hydroxybutyryl-CoA
dehydrogenase-like protein, partial (46%)
Length = 465
Score = 89.7 bits (221), Expect = 1e-18
Identities = 49/139 (35%), Positives = 76/139 (54%)
Frame = +2
Query: 306 VRKAAVIGGGLMGSGIATALILGNIRVILKEVNSEYLQKGIKTIEANVRGLVTRKKLTQQ 365
V++ +GGG MGSGIA + + V L +V+ + L K +I ++ LV++ L+Q
Sbjct: 44 VKRIGGVGGGQMGSGIAQVAAMHGVDVCLFDVDPQALSKASSSISNSINRLVSKSHLSQV 223
Query: 366 KAEGALSLLKGVLDYAEFKDVDMVIEAVIEKVSLKQDIFSDLEKICPPHCILASNTSTID 425
A L+ D EF+D D VIEA++E +K+ +F L+K+ ILASNTS+I
Sbjct: 224 AGADAWKRLRFTSD*EEFRDADFVIEAIVESEDVKKTVFVQLDKMVKSTAILASNTSSIS 403
Query: 426 LNIIGEKISSQDRVIGAHF 444
+ + +VIG HF
Sbjct: 404 ITRLASSTCRPSQVIGMHF 460
>AV780782
Length = 511
Score = 65.9 bits (159), Expect = 2e-11
Identities = 28/43 (65%), Positives = 37/43 (85%)
Frame = -2
Query: 620 EIVEMILFPVVNEACRVLEEGIVIRASDLDIASVLGMSFPSYR 662
+I+EMI FPVVNEACRVL+EGI ++A+DLDI+ ++GM FP R
Sbjct: 510 DIIEMIFFPVVNEACRVLDEGIAVQAADLDISGIMGMGFPPSR 382
>TC15752 similar to PIR|T08956|T08956 AIM1 protein - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (11%)
Length = 520
Score = 50.8 bits (120), Expect = 7e-07
Identities = 24/25 (96%), Positives = 25/25 (100%)
Frame = +1
Query: 638 EEGIVIRASDLDIASVLGMSFPSYR 662
EEG+VIRASDLDIASVLGMSFPSYR
Sbjct: 1 EEGVVIRASDLDIASVLGMSFPSYR 75
>TC14643 similar to UP|Q9T0K7 (Q9T0K7) 3-hydroxyisobutyryl-coenzyme A
hydrolase-like protein (AT4G13360/T9E8_100), complete
Length = 1568
Score = 47.8 bits (112), Expect = 6e-06
Identities = 37/141 (26%), Positives = 70/141 (49%), Gaps = 5/141 (3%)
Frame = +2
Query: 10 VGNDGVAVITMCNPP-VNALAIPIIRGLKNKFEEAARRNDVKAIVLTGKGGR-FSGGFDI 67
V ++GVAVIT+ P +NA+ + + K+ ++ VK +++ R F G DI
Sbjct: 146 VHSNGVAVITLDRPKALNAMNLDMDVKYKSYLDQWELDPRVKCVLVDSSSPRAFCAGMDI 325
Query: 68 S-VMQKVHQTGDITLVPDVSVE--LVVNSIEDSKKPVVAAVEGLALGGGLELAMGCHARV 124
V+ ++ + LV V ++ I + KKP ++ ++G+ +G G+ L+ R+
Sbjct: 326 KGVVAEIQKDKSTPLVQKVFTAEYSLICKIAEYKKPYISFMDGITMGFGIGLSGHGRYRI 505
Query: 125 AAPKAQLGLPELTLGIIPGFG 145
+ L +PE +G+ P G
Sbjct: 506 ITERTVLAMPENGIGLFPDVG 568
>TC16259 weakly similar to UP|O23300 (O23300) Carnitine racemase like
protein (At4g14440), partial (75%)
Length = 910
Score = 44.3 bits (103), Expect = 6e-05
Identities = 61/267 (22%), Positives = 113/267 (41%), Gaps = 24/267 (8%)
Frame = +3
Query: 13 DGVAVITMCNPPVNALAIPIIRGLKNKFEEAARRNDVKAIVLTGKGGRF-SGGFDISVMQ 71
D + V+T+ N L+ +I+ L + + ++++T GRF S GFD++ +
Sbjct: 27 DNLWVLTLTGDDQNRLSPTVIQSLLSTLSRLTSQATPGSVLITTATGRFFSNGFDLTWAR 206
Query: 72 KVHQTGDITLVPDVSVELVVNSIEDSKKPVV-----------AAVEGLALGGGLELAMG- 119
S + S+ DS +PVV AAV G A G LA+
Sbjct: 207 AAGSE---------SAYDRLQSMVDSLRPVVAALVSLPMPTIAAVNGHASAAGFLLAISH 359
Query: 120 CHARVAAPKAQLGLPELTLGI-IPGFGGTQRLPRLVGTSKAVEMMLTSKPITAEEGQKLG 178
+ + + + + +PE+ LG+ +P + ++ + +L I A E K G
Sbjct: 360 DYVLMRSDQGVMYMPEVNLGLPLPDYFAAVMRDKIRSPVTLRDFVLGGVKIRAGEAVKRG 539
Query: 179 LIDAIVSPAELLKLSRQWALEIAEQ--RRPWIRSLH--ITDKLGSDAREVLRTARQHV-- 232
++D+ AE + + A+ + E+ RR W+ ++ I L +A VL ++ +
Sbjct: 540 IVDSAHDGAE---KTVEAAMRLGEELGRRKWVGEVYAEIRKSLYPEACLVLGLTQKSIVS 710
Query: 233 ----KKTAPHLPQQQACIDVIEHGILH 255
+ HL CI +I H ++H
Sbjct: 711 KI*MLRLINHLFTIVLCILIIFHFLIH 791
>TC11677 weakly similar to UP|Q87R67 (Q87R67) Naphthoate synthase, partial
(41%)
Length = 499
Score = 40.4 bits (93), Expect = 0.001
Identities = 24/66 (36%), Positives = 34/66 (51%)
Frame = +1
Query: 146 GTQRLPRLVGTSKAVEMMLTSKPITAEEGQKLGLIDAIVSPAELLKLSRQWALEIAEQRR 205
G+ + RLVG KA EM ++ TA E K+GL++ +V L K + +W EI
Sbjct: 4 GSSIMSRLVGPKKAREMWFLTRFYTAVEADKMGLVNTVVPLENLEKETIKWCREILRNSP 183
Query: 206 PWIRSL 211
IR L
Sbjct: 184 TAIRVL 201
>BP073016
Length = 330
Score = 40.0 bits (92), Expect = 0.001
Identities = 18/27 (66%), Positives = 22/27 (80%)
Frame = -3
Query: 623 EMILFPVVNEACRVLEEGIVIRASDLD 649
EMI F VVNEAC VL+EGI ++ +DLD
Sbjct: 328 EMIFFLVVNEAC*VLDEGIAVKVADLD 248
>TC10228 similar to UP|Q9LDF5 (Q9LDF5) 3-hydroxybutyryl-CoA
dehydrogenase-like protein, partial (44%)
Length = 572
Score = 34.7 bits (78), Expect = 0.050
Identities = 27/94 (28%), Positives = 42/94 (43%), Gaps = 4/94 (4%)
Frame = +3
Query: 457 VRTNKTSAQVILDLVTVGKIIKKSPVVVGNCTGFAVNRTFFPYAQGAHFLANLGV----D 512
VR TS + + + + K+ + + +GF VNR P A F GV D
Sbjct: 3 VRGADTSDETFAATKALSERLGKTVITSQDYSGFIVNRILMPMINEAFFTLYTGVATKED 182
Query: 513 VFRIDRLISNFGLPMGPFQLQDLSGYGVAVAVGK 546
+ +L +N PMGP +L D G V +++ K
Sbjct: 183 IDTGMKLGTNH--PMGPLELADFIGLDVCLSIMK 278
>AV421087
Length = 387
Score = 34.7 bits (78), Expect = 0.050
Identities = 19/64 (29%), Positives = 34/64 (52%)
Frame = +1
Query: 97 SKKPVVAAVEGLALGGGLELAMGCHARVAAPKAQLGLPELTLGIIPGFGGTQRLPRLVGT 156
+ KPV+A++ +A GG +AMG A VA G ++G++ G ++L +G
Sbjct: 97 ASKPVIASMADVAASGGYYMAMGAQAIVAESLTLTG----SIGVVTGKFNLEKLYEKIGF 264
Query: 157 SKAV 160
+K +
Sbjct: 265 NKEI 276
>AV410093
Length = 428
Score = 30.4 bits (67), Expect = 0.95
Identities = 17/37 (45%), Positives = 20/37 (53%)
Frame = +2
Query: 308 KAAVIGGGLMGSGIATALILGNIRVILKEVNSEYLQK 344
+AAVIGGG G+ A AL G I L E N + K
Sbjct: 188 RAAVIGGGPAGASAAEALATGGIETFLFERNPPSVPK 298
>TC14665
Length = 542
Score = 29.3 bits (64), Expect = 2.1
Identities = 23/76 (30%), Positives = 36/76 (47%)
Frame = -2
Query: 67 ISVMQKVHQTGDITLVPDVSVELVVNSIEDSKKPVVAAVEGLALGGGLELAMGCHARVAA 126
+S+ Q Q +++++ + E+ S E KK +GL G + GCH R+A
Sbjct: 505 LSIKQHAIQRANLSII---TTEVTTTSSE--KKK*CPKNQGL---G*EKTT*GCHGRIA* 350
Query: 127 PKAQLGLPELTLGIIP 142
P A PE + IIP
Sbjct: 349 PSAMAKAPEEDINIIP 302
>TC13714 homologue to UP|Q7XA53 (Q7XA53) Sucrose transporter, partial (32%)
Length = 512
Score = 27.7 bits (60), Expect = 6.2
Identities = 17/56 (30%), Positives = 23/56 (40%)
Frame = -2
Query: 121 HARVAAPKAQLGLPELTLGIIPGFGGTQRLPRLVGTSKAVEMMLTSKPITAEEGQK 176
H RV G+ L +G+IP F LP +G + + PI A QK
Sbjct: 457 HHRVQHEAKSTGVNTLIIGVIPNFSAVHFLPHPIGVEQ--QEWEPRDPIEASHEQK 296
>TC9835 similar to AAQ56831 (AAQ56831) At3g03960, partial (67%)
Length = 1126
Score = 27.3 bits (59), Expect = 8.0
Identities = 18/58 (31%), Positives = 31/58 (53%), Gaps = 5/58 (8%)
Frame = +1
Query: 305 NVRKAAVIGGGLMGSGIATALIL-----GNIRVILKEVNSEYLQKGIKTIEANVRGLV 357
NVR A ++GGGL S + ++L G+I+ I K + ++ G+ T + +G V
Sbjct: 556 NVRVAKLLGGGLHNSTVVRGMVLKSDAVGSIKRIEKAKVAVFV-SGVDTSATDTKGTV 726
>TC19171 similar to UP|Q9SXV6 (Q9SXV6) Cycloartenol synthase, partial (18%)
Length = 548
Score = 27.3 bits (59), Expect = 8.0
Identities = 9/23 (39%), Positives = 16/23 (69%)
Frame = +2
Query: 188 ELLKLSRQWALEIAEQRRPWIRS 210
++ ++ + W L+IAE PW+RS
Sbjct: 128 QITQVEQMWKLKIAEGGNPWLRS 196
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.139 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,217,990
Number of Sequences: 28460
Number of extensions: 114108
Number of successful extensions: 523
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 521
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 523
length of query: 662
length of database: 4,897,600
effective HSP length: 96
effective length of query: 566
effective length of database: 2,165,440
effective search space: 1225639040
effective search space used: 1225639040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC134823.6