BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC129715.1 + phase: 0
(416 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12782 403 e-113
BI418878 315 7e-87
TC18403 similar to UP|Q9LJE0 (Q9LJE0) Gb|AAC16072.1 (AT3g13510/M... 147 2e-36
TC10728 124 2e-29
TC13570 weakly similar to UP|Q9SL64 (Q9SL64) At2g20170 protein, ... 72 7e-18
BP049671 85 3e-17
TC19215 weakly similar to UP|Q9XIN9 (Q9XIN9) At2g27320 protein, ... 79 1e-15
AV406564 75 3e-14
AV773405 50 9e-07
AV770854 48 3e-06
TC17791 similar to UP|BAC99644 (BAC99644) Carboxyl-terminal prot... 42 2e-04
TC18655 31 0.33
BG662191 30 0.56
TC15511 30 0.74
TC18558 similar to UP|Q7XAE5 (Q7XAE5) S1 self-incompatibility lo... 28 2.1
TC16200 weakly similar to UP|Q9LVF7 (Q9LVF7) Gb|AAD14441.1, part... 27 6.2
AV766676 27 8.1
>TC12782
Length = 982
Score = 403 bits (1035), Expect = e-113
Identities = 182/224 (81%), Positives = 204/224 (90%)
Frame = +2
Query: 192 KIQQTNEFSLSQIWVLGGSFGQDLNSIEAGWQVSPDLYGDNNTRLFTYWTSDAYQATGCY 251
KIQQ NEFSLSQIW+L G+FGQDLNSIEAGWQVSPDLYGDNNTRLFTYWTSDAYQATGCY
Sbjct: 14 KIQQPNEFSLSQIWILAGAFGQDLNSIEAGWQVSPDLYGDNNTRLFTYWTSDAYQATGCY 193
Query: 252 NLLCSGFIQVSSDIAMGASISPISSYRDSQYDISILIWKDPKEGHWWMQFGNQGTVLGYW 311
NLLCSGFIQ+++DIA+GASISP+S+Y SQYDISIL+WKDP EG+WWMQFGNQ VLGYW
Sbjct: 194 NLLCSGFIQINNDIALGASISPLSNYGSSQYDISILVWKDPNEGNWWMQFGNQ-HVLGYW 370
Query: 312 PSFLFSYLADSATMIEWGGEVVNSEPDGQHTSTQMGSGHFPEEGFGKASYFRNIQVVDSS 371
P+ LFSYL DSA+MIEWGGEVVNSE DGQHTSTQMGSGHFPEEGFGKASYF+NIQ+VD +
Sbjct: 371 PAPLFSYLTDSASMIEWGGEVVNSESDGQHTSTQMGSGHFPEEGFGKASYFKNIQIVDGN 550
Query: 372 NNLKAPKGLGTYTEHPNCYDVQTGSNGDWGHFFYYGGPGKNANC 415
N +APK LGTYTE CY+V+TG+ GDWG++FYYGGPG+N C
Sbjct: 551 NKFRAPKDLGTYTEXNGCYNVKTGNAGDWGNYFYYGGPGRNPXC 682
>BI418878
Length = 609
Score = 315 bits (808), Expect = 7e-87
Identities = 147/178 (82%), Positives = 162/178 (90%)
Frame = +2
Query: 18 VLISVSSSTRLGGSRQKFEVNKHLNRLNKPPVKTIQSPDGDIIDCVPVSKQPAFDHPFLK 77
+L+ +SS+ RL SRQK EVNKHLNRLNKPPVKTIQSPDGDIIDCV VSKQPAFDHPFLK
Sbjct: 74 ILL*ISSAARLSASRQKLEVNKHLNRLNKPPVKTIQSPDGDIIDCVHVSKQPAFDHPFLK 253
Query: 78 DHKIQMRPNFHPEGLFEENKLDDNKEKSSTPINQLWHANGKCSEGTIPIRRTKEEDVLRA 137
DHKIQMRP+FHPEGLF+E KL +NKEK+S PI QLWH+ GKC + TIP+RRTKEEDVLRA
Sbjct: 254 DHKIQMRPSFHPEGLFDETKLSENKEKTSAPITQLWHSIGKCPKDTIPVRRTKEEDVLRA 433
Query: 138 SSAKRYGRKKHKSFAKPRSAEPDLVNQSGHQHAIAYVEGDKFYGAKATINVWEPKIQQ 195
SS KRYGRKKH+S KP+SAEPDL+NQSGHQHAIAYV+GDKFYGAKATINVWEPKIQQ
Sbjct: 434 SSVKRYGRKKHRSIPKPKSAEPDLINQSGHQHAIAYVQGDKFYGAKATINVWEPKIQQ 607
>TC18403 similar to UP|Q9LJE0 (Q9LJE0) Gb|AAC16072.1 (AT3g13510/MRP15_15),
partial (23%)
Length = 416
Score = 147 bits (372), Expect = 2e-36
Identities = 76/138 (55%), Positives = 92/138 (66%), Gaps = 10/138 (7%)
Frame = +2
Query: 127 RRTKEEDVLRASSAKRYGRKKHKSFAKPRS----AEPDLVNQSGHQHAIAYV-----EGD 177
RR+ +DVLRA S +G+K R + D+ + + H+HA+AY
Sbjct: 2 RRSTVDDVLRAKSLYEFGKKPPGMLPLSRRTANVSPNDIFSNNDHEHAVAYTGLGSDSPQ 181
Query: 178 KFYGAKATINVWEPKIQQTNEFSLSQIWVLGGSF-GQDLNSIEAGWQVSPDLYGDNNTRL 236
+ YGAKA+INVWEP IQ NEFSLSQIW+L G F G DLNSIEAGWQVSP +YGD+ RL
Sbjct: 182 EIYGAKASINVWEPSIQVMNEFSLSQIWLLSGFFDGSDLNSIEAGWQVSPQIYGDSRPRL 361
Query: 237 FTYWTSDAYQATGCYNLL 254
FTYWTSD+Y TGCYNLL
Sbjct: 362 FTYWTSDSYGKTGCYNLL 415
>TC10728
Length = 500
Score = 124 bits (312), Expect = 2e-29
Identities = 51/88 (57%), Positives = 68/88 (76%)
Frame = +1
Query: 328 WGGEVVNSEPDGQHTSTQMGSGHFPEEGFGKASYFRNIQVVDSSNNLKAPKGLGTYTEHP 387
WGGEVVN+ + +HTSTQMGSGHF E+G+GKA YFRNI++VD +N L++ + + T E
Sbjct: 1 WGGEVVNTRANDEHTSTQMGSGHFAEDGYGKARYFRNIEIVDVTNTLRSVQNITTIAETS 180
Query: 388 NCYDVQTGSNGDWGHFFYYGGPGKNANC 415
NCY++QT + +WG +FYYGGPGKN C
Sbjct: 181 NCYNIQTSYSKEWGTYFYYGGPGKNPQC 264
>TC13570 weakly similar to UP|Q9SL64 (Q9SL64) At2g20170 protein, partial
(13%)
Length = 505
Score = 72.0 bits (175), Expect(2) = 7e-18
Identities = 29/57 (50%), Positives = 42/57 (72%)
Frame = -3
Query: 31 SRQKFEVNKHLNRLNKPPVKTIQSPDGDIIDCVPVSKQPAFDHPFLKDHKIQMRPNF 87
+ +K E + L +NKPP+ TI + G I+DCV + KQPAFDHP LK+HK+Q++P+F
Sbjct: 473 TEEKLE*ERQLKHINKPPIVTINTT*GYIVDCVDIKKQPAFDHPLLKNHKLQLKPSF 303
Score = 35.0 bits (79), Expect(2) = 7e-18
Identities = 24/92 (26%), Positives = 39/92 (42%), Gaps = 4/92 (4%)
Frame = -1
Query: 118 KCSEGTIPIRRTKEEDVLRASSAKRYGRKKHKSFAKPRSAEPDLVNQSGHQHAIAYVEG- 176
+C GT+P+RRT +ED++R S Y P H G
Sbjct: 229 QCPVGTVPMRRTTKEDLIRGKSLFNY---------------PI*TTGQPGTHIAEVTYGP 95
Query: 177 ---DKFYGAKATINVWEPKIQQTNEFSLSQIW 205
+ YGA + ++W PK++ ++ S S +W
Sbjct: 94 FGFKRVYGASGSGSLWNPKVKH-DQTSNSHMW 2
>BP049671
Length = 488
Score = 84.7 bits (208), Expect = 3e-17
Identities = 32/63 (50%), Positives = 47/63 (73%)
Frame = -3
Query: 353 EEGFGKASYFRNIQVVDSSNNLKAPKGLGTYTEHPNCYDVQTGSNGDWGHFFYYGGPGKN 412
++GFGKASYFRN+++VD+ N+L + + T E+ NCY++++ SN WG +FYYGGPG N
Sbjct: 483 DDGFGKASYFRNLEIVDTDNSLSSVNNILTLAENTNCYNIKSSSNNKWGTYFYYGGPGNN 304
Query: 413 ANC 415
C
Sbjct: 303 PQC 295
>TC19215 weakly similar to UP|Q9XIN9 (Q9XIN9) At2g27320 protein, partial
(12%)
Length = 548
Score = 79.3 bits (194), Expect = 1e-15
Identities = 50/144 (34%), Positives = 75/144 (51%), Gaps = 5/144 (3%)
Frame = +1
Query: 272 SPISSYRDSQYDISILIWKDPKEGHWWMQFGNQGTVLGYWPSFLFSYLADSATMIEWGGE 331
S I S S Y + +D GHWW+ G +GYWP+ L ++L+ +++I +GGE
Sbjct: 19 STIGSDYKSLYPFKVK--QDQSTGHWWLIAGRGSWAVGYWPNPLLTHLSHGSSVIRYGGE 192
Query: 332 VVNSEPDGQHTSTQMGSGHFPEEGFGKASYFRNIQVVDSSNN--LKAPKGLGTYTE-HPN 388
S S MGSG P E + +++ N++++DS+ N PK + + + PN
Sbjct: 193 TYASP---HVMSPPMGSGRLPRELYKNSAFMSNLEIIDSNYNEVRVNPKYMKSNCDTTPN 363
Query: 389 CYDVQ-TGSNG-DWGHFFYYGGPG 410
CYDVQ G G + F YGGPG
Sbjct: 364 CYDVQYPGYEGIVFAQAFLYGGPG 435
>AV406564
Length = 416
Score = 74.7 bits (182), Expect = 3e-14
Identities = 50/154 (32%), Positives = 76/154 (48%), Gaps = 2/154 (1%)
Frame = +2
Query: 58 DIIDCVPVSKQPAFDHPFLKDHKIQMRPNFHPEGLFEENKLDDNKEKSSTPINQLWHANG 117
D DCV + KQPAF HP LK+HKIQ+ P F + DNK+K H+
Sbjct: 5 DDFDCVDLYKQPAFQHPLLKNHKIQLFPTFTMSTM---QLGRDNKDK---------HSRK 148
Query: 118 KCSEGTIPIRRT--KEEDVLRASSAKRYGRKKHKSFAKPRSAEPDLVNQSGHQHAIAYVE 175
+C G +PI +T +E+ + ++S + S ++PR H ++ +
Sbjct: 149 RCPMGKVPIFKTNIREQKINMSTSKSQLDGLNQYSQSRPRH----------HFATLSTTQ 298
Query: 176 GDKFYGAKATINVWEPKIQQTNEFSLSQIWVLGG 209
F+GA A V+ + Q N++SLSQIW+ GG
Sbjct: 299 NMMFHGASAQAAVYNLSL-QANQYSLSQIWIQGG 397
>AV773405
Length = 425
Score = 49.7 bits (117), Expect = 9e-07
Identities = 42/140 (30%), Positives = 62/140 (44%), Gaps = 3/140 (2%)
Frame = +2
Query: 58 DIIDCVPVSKQPAFDHPFLKDHKIQMRPNFHPEGLFEENKLDDNKEKSSTPINQLWHANG 117
D DCV + QPAF HP LK+HKIQ+ P L +K+D K+ N +
Sbjct: 62 DSFDCVDMYNQPAFQHPLLKNHKIQL----FPTCLRTHSKIDPVLGKAVKYQNFI----R 217
Query: 118 KCSEGTIPIRR--TKEEDVLRASSAKRYGRKKHKSFAKPRSAEPDLVNQSGHQHAIAYVE 175
+C G +PI + T+++ V ++SS + S +KP GH A
Sbjct: 218 ECPLGKVPILKTTTRQKMVTKSSSKSQLDNFNENSQSKP-----------GHHFATLETT 364
Query: 176 GD-KFYGAKATINVWEPKIQ 194
D F G A I+ + +Q
Sbjct: 365 KDMMFRGGSARISTYNLSLQ 424
>AV770854
Length = 477
Score = 48.1 bits (113), Expect = 3e-06
Identities = 36/133 (27%), Positives = 54/133 (40%), Gaps = 1/133 (0%)
Frame = +1
Query: 58 DIIDCVPVSKQPAFDHPFLKDHKIQMRPNFHPEGLFEENKLDDNKEKSSTPINQLWHANG 117
D DC+ + KQPAF HP LK+HKIQ+ P +++ + I G
Sbjct: 127 DDFDCIDIYKQPAFQHPLLKNHKIQLFPT------CMRSRMQNRSSYLGKAIKDENSMRG 288
Query: 118 KCSEGTIPIRRTKEEDVLRASSAKRYGRKKHKSFAKPRSAEPDLVNQSGHQHAIAYVEGD 177
C G +PI +T L+ S+ R ++K + GH A D
Sbjct: 289 -CRSGKVPIFKTTMRQKLKTHSSSRSELDNFNQYSK---------S*PGHHFATLDTTQD 438
Query: 178 -KFYGAKATINVW 189
F+GA A + +
Sbjct: 439 ITFHGASARMGTY 477
>TC17791 similar to UP|BAC99644 (BAC99644) Carboxyl-terminal
proteinase-like, partial (4%)
Length = 544
Score = 41.6 bits (96), Expect = 2e-04
Identities = 28/77 (36%), Positives = 41/77 (52%)
Frame = -3
Query: 10 VLVFCLWGVLISVSSSTRLGGSRQKFEVNKHLNRLNKPPVKTIQSPDGDIIDCVPVSKQP 69
++ L+ ++ SVSS+ + G R + NK +L+ + Q D D V + QP
Sbjct: 527 IMAIFLFLLVSSVSSAMK*SGGRIHHQ-NKQQLQLHD--YQNFQPSIDDAWDGVDMYNQP 357
Query: 70 AFDHPFLKDHKIQMRPN 86
AF HP LK+HKIQ PN
Sbjct: 356 AFHHPLLKNHKIQSLPN 306
>TC18655
Length = 506
Score = 31.2 bits (69), Expect = 0.33
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = -3
Query: 83 MRPNFHPEGLFEENKLDDNKEKSSTPINQL 112
++PN+HP L + N L ++ E T +NQL
Sbjct: 252 IQPNYHPPALTDSNTLKNDAENQRTGLNQL 163
>BG662191
Length = 377
Score = 30.4 bits (67), Expect = 0.56
Identities = 26/83 (31%), Positives = 43/83 (51%), Gaps = 1/83 (1%)
Frame = +2
Query: 97 KLDDNKEKSSTPINQLWHANGKCSEGTIPIRRTKEEDVLRASSAKRYGRKKHKSFAKPRS 156
K DD + +P+N+ ++ K E I+++ ++D + AK + HKS A P
Sbjct: 134 KGDDPPAQQKSPMNKEIFSSEKRHESYPIIKKSDDKDQSHSVHAKS-SEQLHKSEAAPDF 310
Query: 157 -AEPDLVNQSGHQHAIAYVEGDK 178
A+ DLVNQS +++ E DK
Sbjct: 311 VAKVDLVNQSASYDSVS-EESDK 376
>TC15511
Length = 1191
Score = 30.0 bits (66), Expect = 0.74
Identities = 23/92 (25%), Positives = 40/92 (43%)
Frame = +1
Query: 80 KIQMRPNFHPEGLFEENKLDDNKEKSSTPINQLWHANGKCSEGTIPIRRTKEEDVLRASS 139
K++++ N P+G + D KE P+ W++NG+ S G K+ + +
Sbjct: 307 KLKIQANLIPKG-----QKYDRKESMQPPVAPHWNSNGRISNGAEINTLQKKAPMFDLNQ 471
Query: 140 AKRYGRKKHKSFAKPRSAEPDLVNQSGHQHAI 171
R +K SF SA P +N H++
Sbjct: 472 NARSLSRKDPSFLS--SAPPLDLNHKDMSHSM 561
>TC18558 similar to UP|Q7XAE5 (Q7XAE5) S1 self-incompatibility locus-linked
pollen 3.15 protein (Fragment), partial (18%)
Length = 639
Score = 28.5 bits (62), Expect = 2.1
Identities = 11/27 (40%), Positives = 16/27 (58%)
Frame = +1
Query: 191 PKIQQTNEFSLSQIWVLGGSFGQDLNS 217
P + N FS +W+LGG+ + LNS
Sbjct: 352 PNLGSMNSFSSLLLWILGGASSEGLNS 432
>TC16200 weakly similar to UP|Q9LVF7 (Q9LVF7) Gb|AAD14441.1, partial (13%)
Length = 737
Score = 26.9 bits (58), Expect = 6.2
Identities = 11/27 (40%), Positives = 16/27 (58%)
Frame = +2
Query: 63 VPVSKQPAFDHPFLKDHKIQMRPNFHP 89
+PVS A +HP LK ++ + PN P
Sbjct: 62 LPVSLARASEHPLLKGRRVLITPNTKP 142
>AV766676
Length = 544
Score = 26.6 bits (57), Expect = 8.1
Identities = 21/80 (26%), Positives = 32/80 (39%)
Frame = +2
Query: 118 KCSEGTIPIRRTKEEDVLRASSAKRYGRKKHKSFAKPRSAEPDLVNQSGHQHAIAYVEGD 177
+C+E + T E + AS K G + S PD V H ++Y G+
Sbjct: 212 RCNEN*VEPSPTPE--FVHAS*GKPLGIYNTSPSLRKNSLLPDFVQSILEPHRLSYKRGN 385
Query: 178 KFYGAKATINVWEPKIQQTN 197
T++ EPKI T+
Sbjct: 386 S*LVRGNTVHCLEPKIWHTS 445
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.135 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,573,496
Number of Sequences: 28460
Number of extensions: 127982
Number of successful extensions: 610
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 602
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 603
length of query: 416
length of database: 4,897,600
effective HSP length: 93
effective length of query: 323
effective length of database: 2,250,820
effective search space: 727014860
effective search space used: 727014860
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC129715.1