BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC126784.4 - phase: 0 /pseudo
(525 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18792 similar to UP|AAD03372 (AAD03372) Expressed protein, par... 338 1e-93
AV422889 230 4e-61
TC17186 homologue to UP|AAD03372 (AAD03372) Expressed protein, p... 72 3e-13
BP068360 49 2e-06
AV764911 48 3e-06
AV409159 28 2.8
BP081760 28 2.8
TC12814 similar to UP|O23503 (O23503) DNA chromosome 4, ESSA I C... 28 2.8
TC11455 similar to UP|Q9ZUS8 (Q9ZUS8) At2g37380 protein, partial... 28 4.8
TC10247 similar to UP|VAG1_ARATH (O82628) Vacuolar ATP synthase ... 28 4.8
AV774234 27 6.3
TC17093 weakly similar to UP|PYRH_LACPL (Q88VJ6) Uridylate kinas... 27 6.3
AV781153 27 6.3
BP035422 27 8.2
AW719598 27 8.2
>TC18792 similar to UP|AAD03372 (AAD03372) Expressed protein, partial (41%)
Length = 886
Score = 338 bits (868), Expect = 1e-93
Identities = 182/301 (60%), Positives = 217/301 (71%), Gaps = 30/301 (9%)
Frame = +1
Query: 40 LNKRHK----------ATND----------ILNEPSPLGLSLRKSPSLLDLIQMTLCQEN 79
LNKRHK A+ND IL+EPSPLGL LRKSPSLLDLIQM L Q
Sbjct: 1 LNKRHKPSSSPLQEWSASNDNSDGSSSQIGILDEPSPLGLRLRKSPSLLDLIQMKLSQGR 180
Query: 80 SVNANTANDNLNSKANKNGRASV------EKLKASNFPATHLKIGSWEYKSKYEGDLVAK 133
S ++NL+S K R + EKLKASNFP + L+IG WEYKS+YEGDLVAK
Sbjct: 181 S------DENLSSGVEKESRGAAAASGAAEKLKASNFPGSLLRIGLWEYKSRYEGDLVAK 342
Query: 134 CYFAKQKLVWEVLEGELKSKIEIQWSDISQLKANCPDDGPSTLTLMVARQPLFFRETNPQ 193
CYFAK KLVWEVLEG LKSKIEIQW+DI LKA CP++G STLT+++ RQPLFFRETNPQ
Sbjct: 343 CYFAKHKLVWEVLEGGLKSKIEIQWADIMALKAQCPENGLSTLTVVLGRQPLFFRETNPQ 522
Query: 194 PRKHTLWQSTTDFTGGQASIHRRHVLQCEQGLLIKHYEKLVQCNDRLKFLSQQPEIMVDS 253
PRKHTLWQ+T DFT GQ S HR H LQC QG+L KH+EKL+QC+ RL FLS+QPEI++ S
Sbjct: 523 PRKHTLWQATADFTNGQCSKHRLHFLQCPQGVLAKHFEKLIQCDLRLNFLSRQPEILMHS 702
Query: 254 PHFDPRSAAIENPHNLKDCDLHQGNG---SAVSCFQNMGSPHSSLS-PSFTTEHSDPSAI 309
PHFD + +A E+P + KD DLHQ +G S+ S F++ GSP +SLS SF EH+DP ++
Sbjct: 703 PHFDTQPSAFEDPGHSKDRDLHQVSGKGSSSTSGFEDRGSPQTSLSFSSFKIEHTDPPSM 882
Query: 310 T 310
T
Sbjct: 883 T 885
>AV422889
Length = 427
Score = 230 bits (587), Expect = 4e-61
Identities = 104/141 (73%), Positives = 125/141 (87%)
Frame = +2
Query: 117 IGSWEYKSKYEGDLVAKCYFAKQKLVWEVLEGELKSKIEIQWSDISQLKANCPDDGPSTL 176
IG+WEYKS+YEGDLVAKCYFAK KLVWEVL+G LK+KIEI WSDI LKAN P+D P TL
Sbjct: 2 IGTWEYKSRYEGDLVAKCYFAKHKLVWEVLDGCLKNKIEIPWSDIMALKANYPEDAPGTL 181
Query: 177 TLMVARQPLFFRETNPQPRKHTLWQSTTDFTGGQASIHRRHVLQCEQGLLIKHYEKLVQC 236
+++AR+PLFFRE NPQPRKHTLWQ+T+DFTGGQASI RRH +QC QGLL KH+EKL+QC
Sbjct: 182 EVVLARRPLFFREINPQPRKHTLWQATSDFTGGQASIQRRHFMQCPQGLLGKHFEKLIQC 361
Query: 237 NDRLKFLSQQPEIMVDSPHFD 257
+ RL FLSQQPE++++SP+F+
Sbjct: 362 DPRLNFLSQQPELVLESPYFE 424
>TC17186 homologue to UP|AAD03372 (AAD03372) Expressed protein, partial (7%)
Length = 595
Score = 71.6 bits (174), Expect = 3e-13
Identities = 37/60 (61%), Positives = 44/60 (72%)
Frame = +3
Query: 466 IEGPNDAKVNIDIKATEENSRDVYGGNPAPGMSRKDSFGDLLLSLPRIASLPKFLFDISE 525
++GP K D+K +EE+S+D GG GMSRKDSF DLLL LPRI SLPKFLF+ISE
Sbjct: 12 VDGPVAEK---DVKPSEEDSKDTSGGKQGLGMSRKDSFSDLLLHLPRITSLPKFLFNISE 182
>BP068360
Length = 475
Score = 49.3 bits (116), Expect = 2e-06
Identities = 31/65 (47%), Positives = 41/65 (62%)
Frame = -1
Query: 461 DNTGFIEGPNDAKVNIDIKATEENSRDVYGGNPAPGMSRKDSFGDLLLSLPRIASLPKFL 520
D+T P A+V ID++ + D +G +P GMSRK+S GDLL +LPRIASLP L
Sbjct: 439 DSTPTRLSPCQAQV-IDLECQPD---DAFGCHPGIGMSRKESAGDLLHNLPRIASLPHSL 272
Query: 521 FDISE 525
F + E
Sbjct: 271 FPMPE 257
>AV764911
Length = 321
Score = 48.1 bits (113), Expect = 3e-06
Identities = 24/39 (61%), Positives = 28/39 (71%)
Frame = -3
Query: 487 DVYGGNPAPGMSRKDSFGDLLLSLPRIASLPKFLFDISE 525
D G N GMSRK+S GDLL +LPR ASLP+FLF + E
Sbjct: 316 DASGSNQGIGMSRKESAGDLLHNLPRKASLPRFLFPMPE 200
>AV409159
Length = 422
Score = 28.5 bits (62), Expect = 2.8
Identities = 17/55 (30%), Positives = 29/55 (51%), Gaps = 12/55 (21%)
Frame = +1
Query: 7 SKNPRSQWESSSATSSIS--------PKFEI----EDSIQDQHAPLNKRHKATND 49
S+NP + +++AT++++ PK I DS++ HAP +RH A D
Sbjct: 37 SQNPNTATATATATATVNGDATLRTAPKKHILPTQPDSLRHLHAPTRRRHPARRD 201
>BP081760
Length = 355
Score = 28.5 bits (62), Expect = 2.8
Identities = 19/73 (26%), Positives = 32/73 (43%), Gaps = 3/73 (4%)
Frame = +2
Query: 14 WESSSATSSISPKFEIEDSIQDQHAPLNKRHKATNDILNEPSPLG--LSLRKSPSLLDL- 70
W + + S +SP F+ + ++ + N IL + PLG L ++ +P LL
Sbjct: 116 WSTCTPESYVSPAFQSSGMLLTGTTHVSSPSSSLNSILKDKGPLGGELEVQTAP*LLSSN 295
Query: 71 IQMTLCQENSVNA 83
+Q C S A
Sbjct: 296 LQTKFCVSQSCTA 334
>TC12814 similar to UP|O23503 (O23503) DNA chromosome 4, ESSA I CONTIG
fragment NO. 6 (Glucosyltransferase like protein),
partial (39%)
Length = 794
Score = 28.5 bits (62), Expect = 2.8
Identities = 10/29 (34%), Positives = 18/29 (61%)
Frame = -2
Query: 462 NTGFIEGPNDAKVNIDIKATEENSRDVYG 490
+ G I+G ND ++ ID++ + +D YG
Sbjct: 421 SNGDIDGSNDNEIGIDVEVRQRPEQDAYG 335
>TC11455 similar to UP|Q9ZUS8 (Q9ZUS8) At2g37380 protein, partial (10%)
Length = 575
Score = 27.7 bits (60), Expect = 4.8
Identities = 25/80 (31%), Positives = 36/80 (44%), Gaps = 5/80 (6%)
Frame = +2
Query: 248 EIMVDSPHFDPRSAAIENP-----HNLKDCDLHQGNGSAVSCFQNMGSPHSSLSPSFTTE 302
EI D + A ++NP H L L N + S N+ PHS+L + +T+
Sbjct: 56 EIERDRETWLGEEAKLQNPTLSLQHPLTLLHLPTSNSPSPSHLANL-PPHSALPMNSSTK 232
Query: 303 HSDPSAITLDSVPCEAPSSS 322
S + L + P APSSS
Sbjct: 233 ASSYLSTFLPASPWSAPSSS 292
>TC10247 similar to UP|VAG1_ARATH (O82628) Vacuolar ATP synthase subunit G 1
(V-ATPase G subunit 1) (Vacuolar proton pump G subunit
1) , complete
Length = 716
Score = 27.7 bits (60), Expect = 4.8
Identities = 15/43 (34%), Positives = 25/43 (57%), Gaps = 2/43 (4%)
Frame = +1
Query: 383 MSDFLGHIEHHISKEM--ASGDPSFSAERLEYQQMMDGITQHL 423
++++ H+EH K++ +SGD + +RLE Q D QHL
Sbjct: 223 IAEYRAHLEHEFQKKVSASSGDSGANVKRLE--QETDAKIQHL 345
>AV774234
Length = 410
Score = 27.3 bits (59), Expect = 6.3
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = -3
Query: 263 IENPHNLKDCDLHQGNGSAVSCFQNMGSPHSSLSP 297
+++PH LK D+HQG+ S PH+S P
Sbjct: 291 VDSPHTLKILDIHQGSALGSS------YPHTSQCP 205
>TC17093 weakly similar to UP|PYRH_LACPL (Q88VJ6) Uridylate kinase (UK)
(Uridine monophosphate kinase) (UMP kinase) , partial
(22%)
Length = 594
Score = 27.3 bits (59), Expect = 6.3
Identities = 17/45 (37%), Positives = 22/45 (48%)
Frame = +3
Query: 61 LRKSPSLLDLIQMTLCQENSVNANTANDNLNSKANKNGRASVEKL 105
+ K S++DL +TLCQEN N NLN N EK+
Sbjct: 93 ITKDLSVMDLTAITLCQEN--NIPVVVFNLNKPGNIEKAIKGEKV 221
>AV781153
Length = 532
Score = 27.3 bits (59), Expect = 6.3
Identities = 23/67 (34%), Positives = 30/67 (44%)
Frame = +2
Query: 26 KFEIEDSIQDQHAPLNKRHKATNDILNEPSPLGLSLRKSPSLLDLIQMTLCQENSVNANT 85
KFE DS +D H K+H ++ +++ L LRK L L M NAN
Sbjct: 305 KFEA-DSFEDIHVINPKKHSSSLELIM*FCNLSFCLRKIHDRLKLCNM--------NANR 457
Query: 86 ANDNLNS 92
N N NS
Sbjct: 458 KNLNTNS 478
>BP035422
Length = 559
Score = 26.9 bits (58), Expect = 8.2
Identities = 19/48 (39%), Positives = 27/48 (55%), Gaps = 2/48 (4%)
Frame = +2
Query: 385 DFLGHIEHHISKEMAS--GDPSFSAERLEYQQMMDGITQHLLNDNQVT 430
DF + + KE AS F A+ ++QQ DG+TQHL+ D +VT
Sbjct: 392 DFRFYFDFEHLKEAASVLDKEQFWADWNKWQQK-DGMTQHLVEDFRVT 532
>AW719598
Length = 454
Score = 26.9 bits (58), Expect = 8.2
Identities = 14/52 (26%), Positives = 22/52 (41%)
Frame = +3
Query: 96 KNGRASVEKLKASNFPATHLKIGSWEYKSKYEGDLVAKCYFAKQKLVWEVLE 147
K + LK + HL+ + +Y GD+ C F +K+ W LE
Sbjct: 204 KESVGRISYLKEICYTQIHLRRI*LLRRRQYVGDMTYSCAFTLRKITWNCLE 359
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.135 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,614,761
Number of Sequences: 28460
Number of extensions: 139306
Number of successful extensions: 685
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 682
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 684
length of query: 525
length of database: 4,897,600
effective HSP length: 94
effective length of query: 431
effective length of database: 2,222,360
effective search space: 957837160
effective search space used: 957837160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC126784.4