BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC125481.10 + phase: 0 /pseudo
(116 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14390 weakly similar to UP|Q9FNL8 (Q9FNL8) Peptide transporter... 117 4e-28
TC13000 similar to PIR|T47573|T47573 peptide transport-like prot... 110 8e-26
TC14955 UP|O22305 (O22305) Peptide transporter, complete 101 3e-23
TC12979 similar to UP|Q9LQL2 (Q9LQL2) F5D14.23 protein, partial ... 94 4e-21
AW720047 85 4e-18
TC13937 weakly similar to UP|Q9LVE0 (Q9LVE0) Nitrate transporter... 84 8e-18
TC14389 weakly similar to UP|Q9FNL8 (Q9FNL8) Peptide transporter... 79 2e-16
BI420182 72 2e-14
BP041397 57 1e-09
AV407586 55 4e-09
AV770189 48 4e-07
CB828533 31 0.060
TC13190 similar to GB|AAL09765.1|15982836|AY057525 At1g27000/T7N... 25 3.3
BP084881 24 5.7
TC8023 23 9.6
>TC14390 weakly similar to UP|Q9FNL8 (Q9FNL8) Peptide transporter, partial
(18%)
Length = 622
Score = 117 bits (294), Expect = 4e-28
Identities = 54/102 (52%), Positives = 73/102 (70%)
Frame = +1
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
T DG VD KGRPVLRS +G W+A FI+ E+ ER AYYGI+SNL+ +L L + T +
Sbjct: 85 TQDGTVDLKGRPVLRSKTGSWKACTFIVGYELFERMAYYGISSNLVVFLGSKLHEGTVAS 264
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYILV 115
+ NV+ W+G PL+GA++AD++LGRY T IIAS +Y+LV
Sbjct: 265 SNNVSNWAGVVWTTPLVGAYIADAYLGRYWTFIIASCIYLLV 390
>TC13000 similar to PIR|T47573|T47573 peptide transport-like protein -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(21%)
Length = 446
Score = 110 bits (274), Expect = 8e-26
Identities = 50/101 (49%), Positives = 70/101 (68%)
Frame = +3
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
T DG VD+ G P + +G W+A FI+ E ER AYYG+++NL+ Y L Q +ATA
Sbjct: 114 TKDGTVDYLGNPANKKQTGTWKACPFILGNECCERLAYYGMSTNLVLYFKKQLNQHSATA 293
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
++NV+ WSGT + PL+GAFLADS+LGRY TI S++Y++
Sbjct: 294 SKNVSNWSGTCYITPLIGAFLADSYLGRYWTIACFSIIYVI 416
>TC14955 UP|O22305 (O22305) Peptide transporter, complete
Length = 2300
Score = 101 bits (252), Expect = 3e-23
Identities = 45/101 (44%), Positives = 69/101 (67%)
Frame = +3
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
T+DG VD GRPVL S +G +A +I+ V ERFAYYG+ +NL+N++T L + ++
Sbjct: 54 TLDGTVDLAGRPVLSSLTGKQKACTYILVYRVLERFAYYGVGANLVNFMTTQLNKDVVSS 233
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
+ N WSG A+L P+LGA++AD++ GR+ TI + L+Y +
Sbjct: 234 ITSFNNWSGLATLTPILGAYIADTYTGRFWTITFSLLIYAI 356
>TC12979 similar to UP|Q9LQL2 (Q9LQL2) F5D14.23 protein, partial (25%)
Length = 601
Score = 94.4 bits (233), Expect = 4e-21
Identities = 45/101 (44%), Positives = 65/101 (63%)
Frame = +3
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
T+DG VD+ GRP +R+ SG W A I+ + A++G+ NL+ +LT LGQ A A
Sbjct: 165 TLDGSVDWHGRPAIRAKSGRWVAGTIILLNQGLATLAFFGVGVNLVLFLTRVLGQDNADA 344
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
A NV+ W+GT L L+GAFL+DS+ GRY+T I +++L
Sbjct: 345 ANNVSKWTGTVYLFSLVGAFLSDSYWGRYKTCAIFQGIFVL 467
>AW720047
Length = 419
Score = 84.7 bits (208), Expect = 4e-18
Identities = 40/81 (49%), Positives = 55/81 (67%)
Frame = +3
Query: 34 WRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAF 93
W+A FI+ ER AYYGI +NL+ YLT L + T +AA+NVN + GT L PL+G+F
Sbjct: 6 WKACPFILGSFFCERLAYYGIATNLVIYLTTKLNEGTVSAAKNVNTFHGTCYLTPLIGSF 185
Query: 94 LADSFLGRYRTIIIASLVYIL 114
LAD++ GRY TI + VY++
Sbjct: 186 LADAYWGRYWTITVFYAVYLI 248
>TC13937 weakly similar to UP|Q9LVE0 (Q9LVE0) Nitrate transporter
(AT3g21670/MIL23_23), partial (27%)
Length = 558
Score = 83.6 bits (205), Expect = 8e-18
Identities = 44/99 (44%), Positives = 61/99 (61%)
Frame = +2
Query: 13 ETVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTAT 72
E + VD+ GRP +S +GGW AA I+ VE+A++ GI+ N++ YL G L TA
Sbjct: 65 EDIAAAVDYLGRPPDKSKTGGWTAAWLILGVELAQKIMVLGISMNIVTYLVGVLNLPTAY 244
Query: 73 AAENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLV 111
+A V GT +L+ LLG FLAD LGRY TI I++ +
Sbjct: 245 SATIVTNCLGTMNLVSLLGGFLADVKLGRYLTIAISATI 361
>TC14389 weakly similar to UP|Q9FNL8 (Q9FNL8) Peptide transporter, partial
(72%)
Length = 1990
Score = 79.0 bits (193), Expect = 2e-16
Identities = 35/75 (46%), Positives = 53/75 (70%)
Frame = +3
Query: 40 IISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADSFL 99
++ E+ ER AYYGI+SNL+ +L L + T ++ NV+ W+G PL+GA++AD++L
Sbjct: 18 LVGYELFERMAYYGISSNLVVFLGSKLHEGTVASSNNVSNWAGVVWTTPLVGAYIADAYL 197
Query: 100 GRYRTIIIASLVYIL 114
GRY T IIAS +Y+L
Sbjct: 198 GRYWTFIIASCIYLL 242
>BI420182
Length = 506
Score = 72.4 bits (176), Expect = 2e-14
Identities = 36/85 (42%), Positives = 53/85 (62%)
Frame = +3
Query: 28 RSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLL 87
R GG FI + E+ E+ A G ++N+I+YLT L AA + + GTASL
Sbjct: 138 RRKQGGLVTMPFIFANEICEKLAVVGFSTNMISYLTTQLHMPLTKAANTLTNFGGTASLT 317
Query: 88 PLLGAFLADSFLGRYRTIIIASLVY 112
PLLGAF++DS+ G++ TI +AS++Y
Sbjct: 318 PLLGAFISDSYAGKFWTITMASVLY 392
>BP041397
Length = 397
Score = 56.6 bits (135), Expect = 1e-09
Identities = 27/69 (39%), Positives = 41/69 (59%)
Frame = +1
Query: 28 RSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLL 87
+ GGW+A +I++ E +R A +G+ +N + YLT L AA VN W G ++
Sbjct: 190 KKQPGGWKAMPYILANETFQRLAIFGLVANFMVYLTRELHLEQVYAATIVNAWFGFSNFG 369
Query: 88 PLLGAFLAD 96
PLLGAF++D
Sbjct: 370 PLLGAFISD 396
>AV407586
Length = 429
Score = 54.7 bits (130), Expect = 4e-09
Identities = 33/103 (32%), Positives = 55/103 (53%)
Frame = +2
Query: 12 LETVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTA 71
+E V+ + +P R GG FII+ E R A G+ N+I YL G
Sbjct: 47 VELAYAAVEDESQPQRRK--GGLITMPFIIANEALARVATIGLLPNMILYLMGTYRLHLG 220
Query: 72 TAAENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
TA + + + S +++ P++GAF+ADS+LGR+ + + S + +L
Sbjct: 221 TATQILLLSSASSNFTPVVGAFIADSYLGRFLAVGLGSAITLL 349
>AV770189
Length = 517
Score = 48.1 bits (113), Expect = 4e-07
Identities = 20/46 (43%), Positives = 29/46 (62%)
Frame = +2
Query: 16 DGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINY 61
+ V+F+G P +S +GGW AA I+ E+AER GI+ NL+ Y
Sbjct: 80 EDAVNFRGHPADKSKTGGWLAAGLILGTELAERICVMGISMNLVTY 217
Score = 45.4 bits (106), Expect = 2e-06
Identities = 26/60 (43%), Positives = 36/60 (59%)
Frame = +1
Query: 52 YGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLV 111
YG L + L G L +A +A V GT +LL LLG FLAD+ LGRY T++I++ +
Sbjct: 187 YGHIHELGDLLVGDLHLHSANSATIVTNCMGTLNLLGLLGGFLADAKLGRYLTVVISATI 366
>CB828533
Length = 557
Score = 30.8 bits (68), Expect = 0.060
Identities = 13/29 (44%), Positives = 18/29 (61%)
Frame = +3
Query: 13 ETVDGVVDFKGRPVLRSSSGGWRAAAFII 41
E + VD+ GRP +S +GGW AA I+
Sbjct: 15 EDIAAAVDYLGRPPDKSKTGGWTAAWLIL 101
>TC13190 similar to GB|AAL09765.1|15982836|AY057525 At1g27000/T7N9_6
{Arabidopsis thaliana;}, partial (46%)
Length = 600
Score = 25.0 bits (53), Expect = 3.3
Identities = 13/42 (30%), Positives = 25/42 (58%)
Frame = -1
Query: 49 FAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLL 90
+ Y+ + L N+++ QST ++ +NI++ T SL+P L
Sbjct: 282 YCYWPV*CQLPNFIS----QSTYLSSNGINIFTFTFSLIPRL 169
>BP084881
Length = 488
Score = 24.3 bits (51), Expect = 5.7
Identities = 8/22 (36%), Positives = 14/22 (63%)
Frame = +3
Query: 13 ETVDGVVDFKGRPVLRSSSGGW 34
+T+ ++DF+ P L+S GW
Sbjct: 198 KTISLIIDFEASPGLQSEGRGW 263
>TC8023
Length = 1089
Score = 23.5 bits (49), Expect = 9.6
Identities = 11/21 (52%), Positives = 12/21 (56%)
Frame = -1
Query: 61 YLTGPLGQSTATAAENVNIWS 81
Y T P G ST AEN + WS
Sbjct: 627 YSTQPQG*STGKIAENRSAWS 565
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.139 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,866,131
Number of Sequences: 28460
Number of extensions: 20390
Number of successful extensions: 101
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 101
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 101
length of query: 116
length of database: 4,897,600
effective HSP length: 78
effective length of query: 38
effective length of database: 2,677,720
effective search space: 101753360
effective search space used: 101753360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 49 (23.5 bits)
Medicago: description of AC125481.10