BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149547.9 + phase: 0
(180 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC227194 similar to UP|Q8T5S8 (Q8T5S8) Prolyl 4-hydroxylase alph... 296 5e-81
TC228929 275 8e-75
TC232158 271 2e-73
TC216902 similar to UP|Q8LAN3 (Q8LAN3) Prolyl 4-hydroxylase alph... 181 2e-46
TC219496 168 1e-42
TC219497 167 2e-42
BE822836 121 2e-28
TC227195 108 1e-24
TC229833 99 8e-22
BE823168 92 1e-19
TC222832 similar to UP|Q75ZI4 (Q75ZI4) Prolyl 4-hydroxylase, par... 90 5e-19
TC222092 similar to UP|Q8VZD7 (Q8VZD7) AT3g28480/MFJ20_16, parti... 89 9e-19
BI971299 82 1e-16
TC227193 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alp... 73 6e-14
AW472148 66 1e-11
TC222216 similar to UP|Q9LSI6 (Q9LSI6) Prolyl 4-hydroxylase alph... 51 3e-07
TC222148 weakly similar to UP|Q75ZI4 (Q75ZI4) Prolyl 4-hydroxyla... 45 1e-05
BE607847 42 2e-04
BM521712 similar to GP|21618073|gb| prolyl 4-hydroxylase alpha s... 40 8e-04
TC234894 weakly similar to UP|ELK3_HUMAN (P41970) ETS-domain pro... 38 0.002
>TC227194 similar to UP|Q8T5S8 (Q8T5S8) Prolyl 4-hydroxylase alpha-related
protein PH4[alpha]PV (CG31015-PA), partial (4%)
Length = 965
Score = 296 bits (757), Expect = 5e-81
Identities = 136/178 (76%), Positives = 152/178 (84%)
Frame = +1
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLH 60
MHKS V+D ETG DSR RTSSG FL RG D+IV++IE+RIA ++FIPVEHGE VLH
Sbjct: 37 MHKSTVVDSETGKSKDSRVRTSSGTFLARGRDKIVRDIEKRIAHYSFIPVEHGEGLQVLH 216
Query: 61 YEVGQKYEPHYDYFMDTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVPWWNE 120
YEVGQKYEPHYDYF+D F+T GQRIAT+LMYL+DVEEGGETVFP AKGNFSSVPWWNE
Sbjct: 217 YEVGQKYEPHYDYFLDDFNTKNGGQRIATVLMYLTDVEEGGETVFPAAKGNFSSVPWWNE 396
Query: 121 LSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMHVGEF 178
LS+CGK GLSIKPK G+A+LFWSMKPDATLDPSSLHG CPVIKG+KW KWM V E+
Sbjct: 397 LSECGKKGLSIKPKRGDALLFWSMKPDATLDPSSLHGGCPVIKGNKWSSTKWMRVSEY 570
>TC228929
Length = 979
Score = 275 bits (703), Expect = 8e-75
Identities = 123/165 (74%), Positives = 143/165 (86%)
Frame = +3
Query: 16 DSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLHYEVGQKYEPHYDYFM 75
+SR RTSSG FLKRG D+I++NIE+RIADFTFIP E+GE +LHYEVGQKYEPHYDYF+
Sbjct: 9 ESRVRTSSGMFLKRGRDKIIQNIEKRIADFTFIPEENGEGLQILHYEVGQKYEPHYDYFL 188
Query: 76 DTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVPWWNELSDCGKGGLSIKPKM 135
D F+T GQRIAT+LMYLSDVEEGGETVFP A NFSSVPWWN+LS C + GLS+KPKM
Sbjct: 189 DEFNTKNGGQRIATVLMYLSDVEEGGETVFPAANANFSSVPWWNDLSQCARKGLSVKPKM 368
Query: 136 GNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMHVGEFKI 180
G+A+LFWSM+PDATLDPSSLHG CPVIKG+KW KWMH+ E+K+
Sbjct: 369 GDALLFWSMRPDATLDPSSLHGGCPVIKGNKWSSTKWMHLREYKV 503
>TC232158
Length = 904
Score = 271 bits (692), Expect = 2e-73
Identities = 126/180 (70%), Positives = 146/180 (81%)
Frame = +2
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLH 60
M KS V D ++G V R S+GAFL RG D IV+NIE+RIAD TFIP+E+GE V+H
Sbjct: 299 MQKSTVADNQSGQSVVHDVRKSTGAFLDRGQDEIVRNIEKRIADVTFIPIENGEPIYVIH 478
Query: 61 YEVGQKYEPHYDYFMDTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVPWWNE 120
YEVGQ Y+PHYDYF+D F+ GQRIATMLMYLS+VEEGGET+FP AK NFSSVPWWNE
Sbjct: 479 YEVGQYYDPHYDYFIDDFNIENGGQRIATMLMYLSNVEEGGETMFPRAKANFSSVPWWNE 658
Query: 121 LSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMHVGEFKI 180
LS+CGK GLSIKPKMG+A+LFWSMKP+ATLD +LH ACPVIKG+KW C KWMH EFK+
Sbjct: 659 LSNCGKMGLSIKPKMGDALLFWSMKPNATLDALTLHSACPVIKGNKWSCTKWMHPTEFKM 838
>TC216902 similar to UP|Q8LAN3 (Q8LAN3) Prolyl 4-hydroxylase alpha
subunit-like protein, partial (89%)
Length = 1381
Score = 181 bits (458), Expect = 2e-46
Identities = 90/184 (48%), Positives = 123/184 (65%), Gaps = 6/184 (3%)
Frame = +3
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLH 60
+ +SAV D +G S RTSSG F+ + D IV IE +I+ +TF+P E+GE+ VL
Sbjct: 387 LKRSAVADNLSGESQLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLR 566
Query: 61 YEVGQKYEPHYDYFMDTFSTTYAGQRIATMLMYLSDVEEGGETVF------PNAKGNFSS 114
YE GQKY+PHYDYF D + G RIAT+LMYL+DV +GGETVF P +G +S
Sbjct: 567 YEHGQKYDPHYDYFTDKVNIARGGHRIATVLMYLTDVAKGGETVFXSAEEPPRRRGAETS 746
Query: 115 VPWWNELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMH 174
++LS+C K G+++KP+ G+A+LF+S+ +AT D SSLH CPVI+G+KW KW+H
Sbjct: 747 ----SDLSECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPVIEGEKWSATKWIH 914
Query: 175 VGEF 178
V F
Sbjct: 915 VDSF 926
>TC219496
Length = 910
Score = 168 bits (426), Expect = 1e-42
Identities = 92/177 (51%), Positives = 113/177 (62%), Gaps = 4/177 (2%)
Frame = +1
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFL--KRGSDRIVKNIERRIADFTFIPVEHGENFNV 58
+H S V+D +TG G+ S RTSSG FL K +V+ IE+RI+ ++ IP+E+GE V
Sbjct: 232 LHISTVVDTKTGKGIKSDVRTSSGMFLNSKERKYPMVQAIEKRISVYSQIPIENGELMQV 411
Query: 59 LHYEVGQKYEPHYDYFMDTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVPWW 118
L YE Q Y+PH+DYF DTF+ GQRIATMLMYLSD EGGET FP A
Sbjct: 412 LRYEKNQYYKPHHDYFSDTFNLKRGGQRIATMLMYLSDNIEGGETYFPLAGS-------- 567
Query: 119 NELSDCGK--GGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWM 173
E S GK GLS+KP GNA+LFWSM D DP+S+HG C VI G+KW KW+
Sbjct: 568 GECSCGGKLVKGLSVKPIKGNAVLFWSMGLDGQSDPNSVHGGCEVISGEKWSATKWL 738
>TC219497
Length = 770
Score = 167 bits (423), Expect = 2e-42
Identities = 92/176 (52%), Positives = 112/176 (63%), Gaps = 4/176 (2%)
Frame = +3
Query: 2 HKSAVIDEETGNGVDSRERTSSGAFLKRGSDR--IVKNIERRIADFTFIPVEHGENFNVL 59
H S V+D +TG G+ S RTSSG FL + +V+ IE+RI+ ++ IP+E+GE VL
Sbjct: 24 HISNVVDTKTGKGIKSDVRTSSGMFLNPQERKYPMVQAIEKRISVYSQIPIENGELMQVL 203
Query: 60 HYEVGQKYEPHYDYFMDTFSTTYAGQRIATMLMYLSDVEEGGETVFPNAKGNFSSVPWWN 119
YE Q Y+PH+DYF DTF+ GQRIATMLMYLSD EGGET FP A
Sbjct: 204 RYEKNQYYKPHHDYFSDTFNLKRGGQRIATMLMYLSDNIEGGETYFPLAGS--------G 359
Query: 120 ELSDCGK--GGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWM 173
E S GK GLS+KP GNA+LFWSM D DP+S+HG C VI G+KW KWM
Sbjct: 360 ECSCGGKLVKGLSVKPIKGNAVLFWSMGLDGQSDPNSVHGGCEVISGEKWSATKWM 527
>BE822836
Length = 610
Score = 121 bits (304), Expect = 2e-28
Identities = 59/126 (46%), Positives = 81/126 (63%)
Frame = -2
Query: 48 IPVEHGENFNVLHYEVGQKYEPHYDYFMDTFSTTYAGQRIATMLMYLSDVEEGGETVFPN 107
IP HG FN+L YEV QKY+ HYD F QRIA+ L+YLS+VE GGET+FP
Sbjct: 606 IPXTHGXIFNILKYEVXQKYDSHYDAFNPDEYXXVESQRIASFLLYLSNVEAGGETMFPY 427
Query: 108 AKGNFSSVPWWNELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKW 167
G ++ + C GL +KP+ G+ +LF+S+ P+ +D +SLHG+CPVIKG+KW
Sbjct: 426 EGG--LNIDRGYDYQKC--IGLKVKPRQGDGLLFYSLLPNGKIDKTSLHGSCPVIKGEKW 259
Query: 168 LCAKWM 173
+ KW+
Sbjct: 258 VATKWI 241
>TC227195
Length = 594
Score = 108 bits (271), Expect = 1e-24
Identities = 48/62 (77%), Positives = 53/62 (85%)
Frame = +2
Query: 118 WNELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMHVGE 177
WNELS+CGK GLSIKPK G+A+LFWSMKPDATLDPSSLHG CPVIKG+K KWM V E
Sbjct: 11 WNELSECGKKGLSIKPKRGDALLFWSMKPDATLDPSSLHGGCPVIKGNKGSSTKWMRVSE 190
Query: 178 FK 179
+K
Sbjct: 191 YK 196
>TC229833
Length = 1265
Score = 99.4 bits (246), Expect = 8e-22
Identities = 57/165 (34%), Positives = 88/165 (52%), Gaps = 8/165 (4%)
Frame = +3
Query: 18 RERTSSGAFLKRG-------SDRIVKNIERRIADFTFIPVEHGENFNVLHYEVGQKYEPH 70
+E++S L G D I+ IE R++ + F+P E+ + V+HY Q +
Sbjct: 381 KEKSSGNGGLSEGVETSLDMEDDILARIEERLSVWAFLPKEYSKPLQVMHYGPEQNGR-N 557
Query: 71 YDYFMDTFSTTYAGQRIATMLMYLS-DVEEGGETVFPNAKGNFSSVPWWNELSDCGKGGL 129
DYF + +G +AT+++YLS DV +GG+ +FP SVP + S C
Sbjct: 558 LDYFTNKTQLELSGPLMATIILYLSNDVTQGGQILFPE------SVPGSSSWSSCSNSSN 719
Query: 130 SIKPKMGNAILFWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMH 174
++P GNAILF+S+ P A+ D SS H CPV++GD W K+ +
Sbjct: 720 ILQPVKGNAILFFSLHPSASPDKSSFHARCPVLEGDMWSAIKYFY 854
>BE823168
Length = 654
Score = 92.4 bits (228), Expect = 1e-19
Identities = 44/98 (44%), Positives = 64/98 (64%), Gaps = 6/98 (6%)
Frame = -1
Query: 87 IATMLMYLSDVEEGGETVF------PNAKGNFSSVPWWNELSDCGKGGLSIKPKMGNAIL 140
+A LMYL+DV +GGETVF P KG+ ++ L +C + G+++KP+ G+A+L
Sbjct: 654 VAXXLMYLTDVXKGGETVFXDAXXSPRHKGSETN----ENLXECAQKGIAVKPRRGDALL 487
Query: 141 FWSMKPDATLDPSSLHGACPVIKGDKWLCAKWMHVGEF 178
F+S+ P+A D SLH CPVI+G+KW KW+HV F
Sbjct: 486 FFSLYPNAIPDTLSLHAGCPVIEGEKWSATKWIHVDSF 373
>TC222832 similar to UP|Q75ZI4 (Q75ZI4) Prolyl 4-hydroxylase, partial (57%)
Length = 619
Score = 90.1 bits (222), Expect = 5e-19
Identities = 49/100 (49%), Positives = 60/100 (60%), Gaps = 7/100 (7%)
Frame = +1
Query: 20 RTSSGAFLKRGSD--RIVKNIERRIADFTFIPVEHGENFNVLHYEVGQKYEPHYDYFMDT 77
RTSSG F+ D R + IE +IA T IP HGE FN+L YEV Q+Y HYD F
Sbjct: 316 RTSSGVFVSASEDKTRTLDVIEEKIARATMIPRSHGEAFNILRYEVNQRYNSHYDAFNPA 495
Query: 78 FSTTYAGQRIATMLMYLSDVEEGGETVFP-----NAKGNF 112
QR+A+ L+YL+DVEEGGET+FP N GN+
Sbjct: 496 EYGPQKSQRMASFLLYLTDVEEGGETMFPFENGLNMDGNY 615
>TC222092 similar to UP|Q8VZD7 (Q8VZD7) AT3g28480/MFJ20_16, partial (26%)
Length = 455
Score = 89.4 bits (220), Expect = 9e-19
Identities = 39/83 (46%), Positives = 57/83 (67%)
Frame = +2
Query: 97 VEEGGETVFPNAKGNFSSVPWWNELSDCGKGGLSIKPKMGNAILFWSMKPDATLDPSSLH 156
VE+GGET+FPNA+ P S+C G ++KP+ G+A+LF+ + DA+ D SLH
Sbjct: 2 VEKGGETIFPNAEAKLLQ-PKDESWSECAHKGYAVKPRKGDALLFFILHLDASTDTKSLH 178
Query: 157 GACPVIKGDKWLCAKWMHVGEFK 179
G+CPVI+G+KW KW+HV +F+
Sbjct: 179 GSCPVIEGEKWSATKWIHVSDFE 247
>BI971299
Length = 669
Score = 82.0 bits (201), Expect = 1e-16
Identities = 48/147 (32%), Positives = 77/147 (51%), Gaps = 4/147 (2%)
Frame = -1
Query: 32 DRIVKNIERRIADFTFIPVEHGENFNVLHYEVGQKYEPH---YDYFMDTFSTTYAGQRIA 88
D I+ IE R++ + E+ + V+HY EP+ DYF +G +A
Sbjct: 657 DDILARIEERLSXXXXLXXEYSKPXQVMHYGP----EPNGRNLDYFTXKTQLELSGPLMA 490
Query: 89 TMLMYLSDVE-EGGETVFPNAKGNFSSVPWWNELSDCGKGGLSIKPKMGNAILFWSMKPD 147
T+++YLS+ +GG+ +FP SVP + S C ++P GNAILF+S+ P
Sbjct: 489 TIVLYLSNAATQGGQILFPE------SVPRSSSWSSCSNSSNILQPVKGNAILFFSLHPS 328
Query: 148 ATLDPSSLHGACPVIKGDKWLCAKWMH 174
A+ D +S H CPV++G+ W K+ +
Sbjct: 327 ASPDKNSFHARCPVLEGNMWSAIKYFY 247
>TC227193 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alpha
subunit-like protein, partial (41%)
Length = 557
Score = 73.2 bits (178), Expect = 6e-14
Identities = 34/51 (66%), Positives = 42/51 (81%)
Frame = +1
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVE 51
MHKS+V+D ETG DSR RTSSG FL RG D+IV++IE+RIA ++FIPVE
Sbjct: 403 MHKSSVVDSETGKSKDSRVRTSSGTFLARGRDKIVRDIEKRIAHYSFIPVE 555
>AW472148
Length = 391
Score = 65.9 bits (159), Expect = 1e-11
Identities = 35/99 (35%), Positives = 56/99 (56%)
Frame = +2
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIPVEHGENFNVLH 60
+ +SAV D + S TSS F+ +D IV +IE +I+ +TF+P+E+ E+ +L
Sbjct: 95 LKRSAVADNLSSESKVSEITTSSCMFIPNNNDLIVASIEYKISSWTFLPIENEEDIQILI 274
Query: 61 YEVGQKYEPHYDYFMDTFSTTYAGQRIATMLMYLSDVEE 99
E G KY PHYDY + T R + +L+ L++V +
Sbjct: 275 CEHGHKYHPHYDYCANKVHITRCTHRSSHILISLTNVTD 391
>TC222216 similar to UP|Q9LSI6 (Q9LSI6) Prolyl 4-hydroxylase alpha
subunit-like protein, partial (27%)
Length = 573
Score = 51.2 bits (121), Expect = 3e-07
Identities = 25/49 (51%), Positives = 31/49 (63%)
Frame = +1
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIP 49
+ KS V D E+G + S RTSSG FL + D IV IE RIA +TF+P
Sbjct: 427 LEKSMVADNESGKSIMSEVRTSSGMFLNKAQDEIVAGIEARIAAWTFLP 573
>TC222148 weakly similar to UP|Q75ZI4 (Q75ZI4) Prolyl 4-hydroxylase, partial
(40%)
Length = 619
Score = 45.4 bits (106), Expect = 1e-05
Identities = 23/45 (51%), Positives = 29/45 (64%), Gaps = 2/45 (4%)
Frame = +1
Query: 20 RTSSGAFLKRGSDR--IVKNIERRIADFTFIPVEHGENFNVLHYE 62
RTSSG F+ D+ I+ +ER+IA T IP HGE FN+L YE
Sbjct: 484 RTSSGTFISASEDKSGILDLVERKIAKVTMIPRTHGEIFNILKYE 618
>BE607847
Length = 482
Score = 42.0 bits (97), Expect = 2e-04
Identities = 24/57 (42%), Positives = 34/57 (59%), Gaps = 5/57 (8%)
Frame = +3
Query: 86 RIATMLMYLSDVEEGGETVFP-----NAKGNFSSVPWWNELSDCGKGGLSIKPKMGN 137
++A+ L+YL+DVEEGGET+FP N GN+ DC GL +KP+ G+
Sbjct: 336 QMASFLLYLTDVEEGGETMFPFENGLNMDGNYG-------YEDC--IGLKVKPRQGD 479
>BM521712 similar to GP|21618073|gb| prolyl 4-hydroxylase alpha subunit-like
protein {Arabidopsis thaliana}, partial (30%)
Length = 412
Score = 39.7 bits (91), Expect = 8e-04
Identities = 19/49 (38%), Positives = 29/49 (58%)
Frame = +1
Query: 1 MHKSAVIDEETGNGVDSRERTSSGAFLKRGSDRIVKNIERRIADFTFIP 49
+ +SAV D +G S RTSSG F+ + D IV +E +I+ +T +P
Sbjct: 253 LKRSAVADNLSGESKLSEVRTSSGMFIPKNKDPIVAGVEDKISSWTLLP 399
>TC234894 weakly similar to UP|ELK3_HUMAN (P41970) ETS-domain protein Elk-3
(ETS-related protein NET) (ETS-related protein ERP) (SRF
accessory protein 2) (SAP-2), partial (5%)
Length = 408
Score = 38.1 bits (87), Expect = 0.002
Identities = 15/23 (65%), Positives = 17/23 (73%)
Frame = +3
Query: 51 EHGENFNVLHYEVGQKYEPHYDY 73
EHGE +V+ Y VGQ YEPH DY
Sbjct: 339 EHGEPLHVIRYAVGQYYEPHVDY 407
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.137 0.431
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,445,112
Number of Sequences: 63676
Number of extensions: 113217
Number of successful extensions: 582
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 569
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 570
length of query: 180
length of database: 12,639,632
effective HSP length: 91
effective length of query: 89
effective length of database: 6,845,116
effective search space: 609215324
effective search space used: 609215324
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC149547.9