BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148758.1 - phase: 0 /pseudo
(143 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC205970 179 5e-46
TC209364 161 9e-41
TC217543 similar to UP|Q940R0 (Q940R0) At1g29690/F15D2_24, parti... 113 3e-26
TC220491 101 1e-22
BM953913 96 6e-21
CA936339 91 2e-19
AW760549 78 1e-15
TC210872 53 6e-08
BG652126 similar to GP|14209545|dbj P0481E12.26 {Oryza sativa (j... 46 7e-06
TC220973 42 1e-04
TC211049 similar to UP|Q9LQV3 (Q9LQV3) F10B6.18, partial (19%) 32 0.079
TC229772 weakly similar to UP|Q6NLR7 (Q6NLR7) At5g04070, partial... 28 1.9
TC228745 similar to UP|O65201 (O65201) Acyl-CoA oxidase , parti... 28 1.9
TC227864 uncoupling protein 1a [Glycine max] 27 2.5
TC234648 27 3.3
TC219244 similar to GB|AAO11608.1|27363378|BT002692 At3g53950/F5... 27 4.3
AW099173 27 4.3
TC219305 weakly similar to UP|Q9LSE7 (Q9LSE7) Emb|CAB45497.1 (AT... 26 7.4
TC210060 26 7.4
TC205588 similar to UP|Q9FH57 (Q9FH57) GATA-binding transcriptio... 26 7.4
>TC205970
Length = 1317
Score = 179 bits (453), Expect = 5e-46
Identities = 88/141 (62%), Positives = 107/141 (75%)
Frame = +3
Query: 1 MEDLHRFLEFQLPRQWAPVLGEIHLGSYRKHQVNTWLRFSILGPKLYINTTPVDVGNRPV 60
+E+LH+FLEFQLPRQWAPV GE+ LG RK Q L+FS +GPKLY+NTTPVDVG +PV
Sbjct: 171 IEELHQFLEFQLPRQWAPVFGELALGPERKPQNTASLQFSFMGPKLYVNTTPVDVGKKPV 350
Query: 61 TGLRLQLEGSRSNRLAIHLQHLASLPKSLPLADNANAYLSCDSYSCTFHKKVKRNCFSYV 120
TGLRL LEG RSN LAIHLQHL+SLPK+ L D N S DS +++KV+ FS+V
Sbjct: 351 TGLRLYLEGKRSNCLAIHLQHLSSLPKTFQLQDEPNGNASNDSSERKYYEKVQWKSFSHV 530
Query: 121 CTAPVESDDSLSIVTGAQLQV 141
CTAPV+SDD ++VTGA +V
Sbjct: 531 CTAPVDSDDDNAVVTGAHFEV 593
>TC209364
Length = 725
Score = 161 bits (408), Expect = 9e-41
Identities = 79/94 (84%), Positives = 84/94 (89%)
Frame = +2
Query: 49 NTTPVDVGNRPVTGLRLQLEGSRSNRLAIHLQHLASLPKSLPLADNANAYLSCDSYSCTF 108
NT PVDVGNRPV GLRLQLEG SNRLAIHLQHLASLPKSL ++DN+NAYLSCDSY+C
Sbjct: 2 NTIPVDVGNRPVVGLRLQLEGRSSNRLAIHLQHLASLPKSLSVSDNSNAYLSCDSYNCNL 181
Query: 109 HKKVKRNCFSYVCTAPVESDDSLSIVTGAQLQVE 142
HKKVK N SYVCTAPVESDDS+SIVTGAQLQVE
Sbjct: 182 HKKVKWNSLSYVCTAPVESDDSVSIVTGAQLQVE 283
>TC217543 similar to UP|Q940R0 (Q940R0) At1g29690/F15D2_24, partial (29%)
Length = 1093
Score = 113 bits (283), Expect = 3e-26
Identities = 68/154 (44%), Positives = 92/154 (59%), Gaps = 13/154 (8%)
Frame = +1
Query: 1 MEDLHRFLEFQLPRQWAPVLGEIHLGSYRKHQVNTWLRFSILGPKLYINTTPVDVGNRPV 60
+EDL FL+FQ+ R WAP + RK V L+FS++GPKL+++ V VG +PV
Sbjct: 133 IEDLQYFLDFQITRVWAPEQNNLQ----RKEPVCQSLQFSLMGPKLFVSPDQVTVGRKPV 300
Query: 61 TGLRLQLEGSRSNRLAIHLQHLASLPKSLP------LADNANAYLSCDSYSCTFHKKVKR 114
TGLRL LEGS+ NRLAIHLQHL SLPK+L +A A + + + + +K
Sbjct: 301 TGLRLSLEGSKQNRLAIHLQHLVSLPKNLQPHWDAHMAIGAPKWHGPEEQDSRWFEPIKW 480
Query: 115 NCFSYVCTAPVESDDS-------LSIVTGAQLQV 141
FS+V TAP+E ++ + IVTGAQL V
Sbjct: 481 KNFSHVSTAPIEYTETSIGDLSGVHIVTGAQLGV 582
>TC220491
Length = 875
Score = 101 bits (251), Expect = 1e-22
Identities = 59/148 (39%), Positives = 88/148 (58%), Gaps = 10/148 (6%)
Frame = +2
Query: 2 EDLHRFLEFQLPRQWAPVLGEIHLGSYRKHQVNTWLRFSILGPKLYINTTPVDVGNRPVT 61
+DL FLEFQ+PRQWAP+ ++ L RK + L+F + PKL+++ V +PV
Sbjct: 371 DDLQCFLEFQIPRQWAPMFYDLPLRHQRKKCSSPSLQFGFMFPKLHVSCAQVVSEQKPVV 550
Query: 62 GLRLQLEGSRSNRLAIHLQHLASLPKSLPLADNANAYLSCD--SYSCTFHKKVKRNCFSY 119
GLRL LEG +S+RLAIH+ HL+SLP ++ + +++ D S F + ++ F+
Sbjct: 551 GLRLYLEGRKSDRLAIHVHHLSSLPNTMIYSSGTSSWRGSDDNESSDIFLEPIRWKGFAN 730
Query: 120 VCTAPVESDDS--------LSIVTGAQL 139
VCTA V+ D + + IVTGAQL
Sbjct: 731 VCTAVVKHDPTWLQETSGGVYIVTGAQL 814
>BM953913
Length = 421
Score = 95.9 bits (237), Expect = 6e-21
Identities = 49/89 (55%), Positives = 63/89 (70%)
Frame = +2
Query: 1 MEDLHRFLEFQLPRQWAPVLGEIHLGSYRKHQVNTWLRFSILGPKLYINTTPVDVGNRPV 60
+E+L FLEFQ+P WAP+ +I G RK V L+FSI+G KLY++ + VG RPV
Sbjct: 149 IEELRYFLEFQIPCVWAPLQDKIP-GQQRKEPVCPSLQFSIMGQKLYVSQEQITVGRRPV 325
Query: 61 TGLRLQLEGSRSNRLAIHLQHLASLPKSL 89
TGL L LEGS+ NRL++H+QHL SLPK L
Sbjct: 326 TGLHLCLEGSKQNRLSVHVQHLVSLPKIL 412
>CA936339
Length = 431
Score = 90.5 bits (223), Expect = 2e-19
Identities = 46/86 (53%), Positives = 60/86 (69%)
Frame = +1
Query: 1 MEDLHRFLEFQLPRQWAPVLGEIHLGSYRKHQVNTWLRFSILGPKLYINTTPVDVGNRPV 60
+E+L FLEFQ+ WAP+ I G RK + L+FSI+G KLY++ + VG PV
Sbjct: 175 IEELRYFLEFQIAHVWAPLHERIP-GQQRKEPICPSLQFSIMGQKLYVSQEQITVGRLPV 351
Query: 61 TGLRLQLEGSRSNRLAIHLQHLASLP 86
TGLRL LEGS+ NRL++HLQHL+SLP
Sbjct: 352 TGLRLFLEGSKQNRLSVHLQHLSSLP 429
>AW760549
Length = 296
Score = 78.2 bits (191), Expect = 1e-15
Identities = 34/43 (79%), Positives = 38/43 (88%)
Frame = +3
Query: 1 MEDLHRFLEFQLPRQWAPVLGEIHLGSYRKHQVNTWLRFSILG 43
+EDLH+FLEFQLPR WAPV EI LGS+ KHQVNTW+RFSILG
Sbjct: 168 IEDLHQFLEFQLPRHWAPVASEISLGSHHKHQVNTWIRFSILG 296
>TC210872
Length = 484
Score = 52.8 bits (125), Expect = 6e-08
Identities = 25/35 (71%), Positives = 31/35 (88%)
Frame = +3
Query: 108 FHKKVKRNCFSYVCTAPVESDDSLSIVTGAQLQVE 142
F++KV+ FS+VCTAPVES++ LSIVTGAQLQVE
Sbjct: 6 FYEKVQWKNFSHVCTAPVESEEDLSIVTGAQLQVE 110
>BG652126 similar to GP|14209545|dbj P0481E12.26 {Oryza sativa (japonica
cultivar-group)}, partial (12%)
Length = 239
Score = 45.8 bits (107), Expect = 7e-06
Identities = 28/74 (37%), Positives = 40/74 (53%), Gaps = 6/74 (8%)
Frame = +2
Query: 58 RPVTGLRLQLEGSRSNRLAIHLQHLASLPKSLPLADNANAYLSCDSYSCTFHKKVKR--- 114
RPVTGL L L S+ NRL++H+QHL SLPK L + + + + ++ +R
Sbjct: 5 RPVTGLCLCLAVSKQNRLSVHVQHLVSLPKILHPYWDGDVAIGAPKWQGPEEQESRRLGA 184
Query: 115 ---NCFSYVCTAPV 125
F YV TAP+
Sbjct: 185 C*GKNFFYVSTAPI 226
>TC220973
Length = 986
Score = 41.6 bits (96), Expect = 1e-04
Identities = 29/80 (36%), Positives = 43/80 (53%), Gaps = 13/80 (16%)
Frame = +2
Query: 75 LAIHLQHLASLPKSL------PLADNANAYLSCDSYSCTFHKKVKRNCFSYVCTAPVESD 128
L++H+QHL SLPK L +A A + + + + VK FS+V TAP+E+
Sbjct: 2 LSVHVQHLVSLPKILHPYWDSHVAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENP 181
Query: 129 DS-------LSIVTGAQLQV 141
++ + IVTGAQL V
Sbjct: 182 ETFIGDFSGVYIVTGAQLGV 241
>TC211049 similar to UP|Q9LQV3 (Q9LQV3) F10B6.18, partial (19%)
Length = 668
Score = 32.3 bits (72), Expect = 0.079
Identities = 19/43 (44%), Positives = 24/43 (55%), Gaps = 7/43 (16%)
Frame = +1
Query: 108 FHKKVKRNCFSYVCTAPVESD-------DSLSIVTGAQLQVEK 143
F + + F +VCTAPV+ D D IVTGAQL V+K
Sbjct: 10 FFEAINGKKFYHVCTAPVKYDPRWSSDKDVAFIVTGAQLHVKK 138
Score = 26.6 bits (57), Expect = 4.3
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = -1
Query: 17 APVLGEIHLGSYRKHQVNTWLRF 39
A L E+HLGSY V+TW F
Sbjct: 101 ATSLSELHLGSYFTGAVHTW*NF 33
>TC229772 weakly similar to UP|Q6NLR7 (Q6NLR7) At5g04070, partial (57%)
Length = 935
Score = 27.7 bits (60), Expect = 1.9
Identities = 12/42 (28%), Positives = 23/42 (54%)
Frame = -3
Query: 83 ASLPKSLPLADNANAYLSCDSYSCTFHKKVKRNCFSYVCTAP 124
AS+ +S P + + N+ ++Y+ + +C S+VCT P
Sbjct: 633 ASIIESTPHSGDCNSCRRFNTYNANQDRIAGTSCMSFVCTTP 508
>TC228745 similar to UP|O65201 (O65201) Acyl-CoA oxidase , partial (68%)
Length = 1796
Score = 27.7 bits (60), Expect = 1.9
Identities = 19/46 (41%), Positives = 24/46 (51%), Gaps = 2/46 (4%)
Frame = -2
Query: 66 QLEGSRSNRLAIHLQHLASLP--KSLPLADNANAYLSCDSYSCTFH 109
QL S RL L++ LP + +PL AYL + YSCTFH
Sbjct: 412 QLWQQPSGRL---LRNTQGLPYHQQVPLE*RQTAYLL*EGYSCTFH 284
>TC227864 uncoupling protein 1a [Glycine max]
Length = 1434
Score = 27.3 bits (59), Expect = 2.5
Identities = 20/61 (32%), Positives = 30/61 (48%), Gaps = 1/61 (1%)
Frame = +1
Query: 68 EGSRSNRLAIHLQHLASLPKSLPLADN-ANAYLSCDSYSCTFHKKVKRNCFSYVCTAPVE 126
EG SN +H Q L P +AD+ +N+ LS + F CF+ VCT P++
Sbjct: 157 EGKDSN---LHTQPLVPFPTLTMVADSKSNSDLS---FGKIFASSAFSACFAEVCTIPLD 318
Query: 127 S 127
+
Sbjct: 319 T 321
>TC234648
Length = 626
Score = 26.9 bits (58), Expect = 3.3
Identities = 14/39 (35%), Positives = 22/39 (55%)
Frame = -3
Query: 76 AIHLQHLASLPKSLPLADNANAYLSCDSYSCTFHKKVKR 114
A+ L L LPKSL +++ +C + S HKK+K+
Sbjct: 150 ALPLPKLFCLPKSLTISNPNFPKSACGAISDQLHKKIKQ 34
>TC219244 similar to GB|AAO11608.1|27363378|BT002692 At3g53950/F5K20_250
{Arabidopsis thaliana;} , partial (34%)
Length = 858
Score = 26.6 bits (57), Expect = 4.3
Identities = 17/55 (30%), Positives = 27/55 (48%)
Frame = -2
Query: 65 LQLEGSRSNRLAIHLQHLASLPKSLPLADNANAYLSCDSYSCTFHKKVKRNCFSY 119
L + G + N AIH HL + +P+ + + L+ S +C KK+ N F Y
Sbjct: 803 LLVVGLKKNEFAIHQSHLPASQSPIPINN*RISILNF*SPTCPPKKKI--NKFKY 645
>AW099173
Length = 385
Score = 26.6 bits (57), Expect = 4.3
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +2
Query: 78 HLQHLASLPKSLPLADNANAYLSCDSYSCTFHKKVKRNCF 117
H+ L SL PL++ +N + S + FH K +C+
Sbjct: 113 HIDALCSLEDGFPLSERSNRPRTSRSENRLFHFKTNYSCY 232
>TC219305 weakly similar to UP|Q9LSE7 (Q9LSE7) Emb|CAB45497.1
(AT3g25290/MJL12_25), partial (39%)
Length = 627
Score = 25.8 bits (55), Expect = 7.4
Identities = 22/71 (30%), Positives = 33/71 (45%), Gaps = 3/71 (4%)
Frame = +2
Query: 16 WAPVLGEIHLGSYRKHQVNTWLRF---SILGPKLYINTTPVDVGNRPVTGLRLQLEGSRS 72
W P + H G + + TW SI LY N P+ V N T R+ E S
Sbjct: 68 WLPGIVLRHWGCWLGNWHETWKPIRGDSIQCSSLYWNL-PLFVCNS--TDFRIVPEASEG 238
Query: 73 NRLAIHLQHLA 83
+++ +HL+HL+
Sbjct: 239 SQV*VHLEHLS 271
>TC210060
Length = 759
Score = 25.8 bits (55), Expect = 7.4
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = -3
Query: 104 YSCTFHKKVKRNCFSYVCTAPVESDDSLSIVTG 136
YSC F +++KR FSY T S S S+ G
Sbjct: 259 YSCKFDQELKR*TFSYYHTHT*TSSHSASLHFG 161
>TC205588 similar to UP|Q9FH57 (Q9FH57) GATA-binding transcription
factor-like protein (At5g66320), partial (25%)
Length = 1084
Score = 25.8 bits (55), Expect = 7.4
Identities = 22/78 (28%), Positives = 32/78 (40%)
Frame = -1
Query: 29 RKHQVNTWLRFSILGPKLYINTTPVDVGNRPVTGLRLQLEGSRSNRLAIHLQHLASLPKS 88
R + + FS KL I + VTG +E S S + L+ L+S P+S
Sbjct: 262 RSSKSPAYTNFSYQKTKLEIE---IGTNGERVTGTANSVEKSES*ETPVELELLSSSPRS 92
Query: 89 LPLADNANAYLSCDSYSC 106
D A+ S+SC
Sbjct: 91 CD-CDEADKLCFSSSHSC 41
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.136 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,372,561
Number of Sequences: 63676
Number of extensions: 110179
Number of successful extensions: 615
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 606
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 611
length of query: 143
length of database: 12,639,632
effective HSP length: 88
effective length of query: 55
effective length of database: 7,036,144
effective search space: 386987920
effective search space used: 386987920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC148758.1