BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148718.5 + phase: 0
(344 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC230717 similar to GB|AAP21346.1|30102856|BT006538 At3g60590 {A... 319 1e-87
TC230718 similar to UP|Q6RFM9 (Q6RFM9) NADH dehydrogenase subuni... 258 3e-69
TC233693 similar to UP|Q9FM48 (Q9FM48) Emb|CAB81845.1, partial (... 39 0.003
TC217322 35 0.065
TC216762 similar to UP|Q6NM69 (Q6NM69) At5g46870, partial (73%) 34 0.11
BE659962 33 0.14
TC231969 similar to GB|AAM55302.1|28624758|AY115841 auxin influx... 31 0.93
TC232747 31 0.93
TC217324 30 1.6
AW101280 30 1.6
BU549065 30 2.1
TC206170 UP|CAP1_SOYBN (Q02909) Phosphoenolpyruvate carboxylase,... 29 2.7
TC230659 weakly similar to UP|O24088 (O24088) MtN24 protein, par... 29 2.7
TC210299 homologue to UP|EFT2_SOYBN (P46280) Elongation factor T... 29 2.7
TC217356 similar to UP|Q84JM5 (Q84JM5) Angustifolia, partial (57%) 26 3.1
AI442632 29 3.6
TC224890 similar to UP|TIP2_TOBAC (P24422) Probable aquaporin TI... 29 3.6
TC224822 similar to UP|TIP2_TOBAC (P24422) Probable aquaporin TI... 29 3.6
TC208709 similar to UP|Q8S9G8 (Q8S9G8) AT5g18830/F17K4_80, parti... 28 4.6
NP004888 putative cytochrome P450 28 4.6
>TC230717 similar to GB|AAP21346.1|30102856|BT006538 At3g60590 {Arabidopsis
thaliana;} , partial (61%)
Length = 685
Score = 319 bits (818), Expect = 1e-87
Identities = 153/180 (85%), Positives = 168/180 (93%)
Frame = +2
Query: 161 LDCIFNLGATLFLLMADSCSRPKNPTQEIKSKAPFSYQFWNIVATLTGFIVPSLLMFGSQ 220
LDCIFNLGATLFLLMAD+CSRPK+ TQ+ KSKAPFSYQFWNIVATLTGFI+P L+MFGSQ
Sbjct: 2 LDCIFNLGATLFLLMADACSRPKSLTQDCKSKAPFSYQFWNIVATLTGFIIPLLMMFGSQ 181
Query: 221 KGFLQPQLPFISSAVLLGPYLLLLSVQILTELLTWYWQSPVWLVTPIIYEAYRILQLMRG 280
KGFLQPQLPFI AVLLGPYLLLLSVQ LTE+LTW+WQSPVWLVTP+IYE+YR+LQLMRG
Sbjct: 182 KGFLQPQLPFIPFAVLLGPYLLLLSVQFLTEMLTWHWQSPVWLVTPVIYESYRVLQLMRG 361
Query: 281 LKLGAELTAPAWMMHTIRGLVCWWVLILGLQLMRVAWFAGLSARARKDQSSSSEASTANG 340
LKLG EL+APAW+MHTIRGLVCWWVLILGLQLMRVAWFAGL+AR RK Q SS+ S+ANG
Sbjct: 362 LKLGVELSAPAWVMHTIRGLVCWWVLILGLQLMRVAWFAGLAARTRK-QQLSSDTSSANG 538
>TC230718 similar to UP|Q6RFM9 (Q6RFM9) NADH dehydrogenase subunit F
(Fragment), partial (3%)
Length = 607
Score = 258 bits (659), Expect = 3e-69
Identities = 127/171 (74%), Positives = 141/171 (82%)
Frame = +1
Query: 3 SSLIHTHYKLHTSTFERAYPRSHGLLKSGKPFQSRRFNFPSIRLNQSFICCTKLTPWEPS 62
+SL HYK+HTSTF R + RS GLLKSGK Q + FPSI +NQS ICCTKLTPWE S
Sbjct: 97 ASLTQLHYKVHTSTFRRVHSRSQGLLKSGKLSQLQGSAFPSIHINQSCICCTKLTPWESS 276
Query: 63 PGVAYAPTDNQSDNFLQSTASVFETLESSKVDESPTANVEGLVEEKDRPGPELQLFKWPM 122
P V YAPTDNQSD FL A++FETLESSK ESP N EG+VE + +PG +LQ+FKWP+
Sbjct: 277 P-VTYAPTDNQSDTFLPQNANIFETLESSKTAESPITNAEGVVETESQPGLQLQVFKWPL 453
Query: 123 WLLGPSILLATGMVPTLWLPISSIFLGPNIASLLSLIGLDCIFNLGATLFL 173
WLLGPS+LLATGMVPTLWLPISSIFLGPNIASLLSLIGLDCIFNLGATLFL
Sbjct: 454 WLLGPSVLLATGMVPTLWLPISSIFLGPNIASLLSLIGLDCIFNLGATLFL 606
>TC233693 similar to UP|Q9FM48 (Q9FM48) Emb|CAB81845.1, partial (18%)
Length = 662
Score = 38.9 bits (89), Expect = 0.003
Identities = 35/126 (27%), Positives = 54/126 (42%), Gaps = 21/126 (16%)
Frame = +1
Query: 78 LQSTASVFETLESSKVDESPTANV----------EGLVEEKDRPGPELQLFKWP------ 121
L++TA V E + D SP +N GL+ +RP P
Sbjct: 283 LETTALVLENSNTVAEDISPASNSYFHLSGSDGKPGLLSFYNRPYRRDSKILLPNSERSQ 462
Query: 122 ---MWLLGPSILLATGMVPTLWL--PISSIFLGPNIASLLSLIGLDCIFNLGATLFLLMA 176
+W LGP++L+A+ + P+L+L +S IF + L L + IF G +FL +
Sbjct: 463 NSILWFLGPAVLVASFIFPSLYLRKVLSIIFEDSLLTDFLILFFTEAIFYCGDGVFLYLL 642
Query: 177 DSCSRP 182
RP
Sbjct: 643 XHVRRP 660
>TC217322
Length = 1077
Score = 34.7 bits (78), Expect = 0.065
Identities = 33/126 (26%), Positives = 54/126 (42%), Gaps = 12/126 (9%)
Frame = +1
Query: 161 LDCIFNLGATLFLLMADSCSRPKNPTQEIKS---------KAPFSYQFWNIVATLTGFIV 211
LD +F A+ ++L + P + + +S K ++ + +V G +
Sbjct: 313 LDLVFPALASAYILALSLFAFPSSSSSSTRSLHDSGTEIFKGGRLFRMYVVVGITVGLFL 492
Query: 212 PSLLMFGSQKGFLQPQLPFISSAVLLGPYLLLLSVQILTELLTW---YWQSPVWLVTPII 268
P + G GF + + SA P+L LLS QILTE + + PV + P+I
Sbjct: 493 PLAYVLG---GFARGDEHAVRSAT---PHLFLLSFQILTENIIGGLSMFSPPVRALVPMI 654
Query: 269 YEAYRI 274
Y RI
Sbjct: 655 YTVRRI 672
>TC216762 similar to UP|Q6NM69 (Q6NM69) At5g46870, partial (73%)
Length = 1241
Score = 33.9 bits (76), Expect = 0.11
Identities = 24/63 (38%), Positives = 30/63 (47%)
Frame = -1
Query: 187 QEIKSKAPFSYQFWNIVATLTGFIVPSLLMFGSQKGFLQPQLPFISSAVLLGPYLLLLSV 246
++IK P Q W I A L LL+ G+QKG + LP S VLL L L S
Sbjct: 1112 KQIKGSNPGQEQAWKISAHLL------LLLLGTQKGLVHIDLPLFVSVVLLHSPLSLSSS 951
Query: 247 QIL 249
+L
Sbjct: 950 VLL 942
>BE659962
Length = 667
Score = 33.5 bits (75), Expect = 0.14
Identities = 26/87 (29%), Positives = 42/87 (47%), Gaps = 3/87 (3%)
Frame = +3
Query: 197 YQFWNIVATLTGFIVPSLLMFGSQKGFLQPQLPFISSAVLLGPYLLLLSVQILTELLTWY 256
+Q + +V T G +P + G GF + + SA P+L LL+ QILTE +
Sbjct: 336 FQVYVVVGTTVGLFLPLAYVLG---GFGRGDKQAVRSA---SPHLFLLAFQILTENVISS 497
Query: 257 W---QSPVWLVTPIIYEAYRILQLMRG 280
+ PV + P++Y R L ++ G
Sbjct: 498 FSLVSPPVRAMVPLMYTVPRTLWILGG 578
>TC231969 similar to GB|AAM55302.1|28624758|AY115841 auxin influx carrier
protein {Medicago truncatula;} , partial (32%)
Length = 644
Score = 30.8 bits (68), Expect = 0.93
Identities = 24/104 (23%), Positives = 45/104 (43%)
Frame = +2
Query: 135 MVPTLWLPISSIFLGPNIASLLSLIGLDCIFNLGATLFLLMADSCSRPKNPTQEIKSKAP 194
++P +L I F GP +++ +L+ ++ + A+ +L S S +N + K P
Sbjct: 110 VIPIWFLAIIFPFFGPINSAVGALLVSFTVYIIPASAHMLTYRSASARQNAAE----KLP 277
Query: 195 FSYQFWNIVATLTGFIVPSLLMFGSQKGFLQPQLPFISSAVLLG 238
F W ++ + F+V +L+ G G FI G
Sbjct: 278 FFIPNWTVMYVVNAFVVVWVLVVGFGFGGWASMTNFIKQVDTFG 409
>TC232747
Length = 681
Score = 30.8 bits (68), Expect = 0.93
Identities = 10/19 (52%), Positives = 13/19 (67%)
Frame = -1
Query: 52 CCTKLTPWEPSPGVAYAPT 70
CCTKL PW +P +AP+
Sbjct: 231 CCTKLQPWHSNPWTPFAPS 175
>TC217324
Length = 545
Score = 30.0 bits (66), Expect = 1.6
Identities = 13/39 (33%), Positives = 22/39 (56%), Gaps = 4/39 (10%)
Frame = +2
Query: 257 WQSPVWLVTPIIYEAY----RILQLMRGLKLGAELTAPA 291
WQ P+WL++P+IY +L+RG+ E + P+
Sbjct: 161 WQWPIWLISPLIYLGS*YPGSFQELLRGISKRRERSIPS 277
>AW101280
Length = 422
Score = 30.0 bits (66), Expect = 1.6
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = +1
Query: 30 SGKPFQSRRFNFPSIRLNQSFICCTKLTPWEPSPGVAYAPTDNQ 73
SG P + F PSI + F C + PWE +++ P D Q
Sbjct: 196 SGFPDLTLSFTNPSILNDVRFHPCVRFRPWESHQILSFVPPDGQ 327
>BU549065
Length = 690
Score = 29.6 bits (65), Expect = 2.1
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +3
Query: 52 CCTKLTPWEPSPGVAYAP 69
CCTKL PW +P + + P
Sbjct: 489 CCTKLQPWHSNPWIPFEP 542
>TC206170 UP|CAP1_SOYBN (Q02909) Phosphoenolpyruvate carboxylase, housekeeping
isozyme (PEPCase) , complete
Length = 3286
Score = 29.3 bits (64), Expect = 2.7
Identities = 13/28 (46%), Positives = 16/28 (56%), Gaps = 1/28 (3%)
Frame = -2
Query: 37 RRFNFPSIRLNQSFICCTKLTP-WEPSP 63
+ N P+I LN F CCTK P W+ P
Sbjct: 2427 QHMNIPNILLNNMFECCTKA*PHWKMEP 2344
>TC230659 weakly similar to UP|O24088 (O24088) MtN24 protein, partial (44%)
Length = 1181
Score = 29.3 bits (64), Expect = 2.7
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +3
Query: 275 LQLMRGLKLGAELTAPAWMMHTIRGLVCWWVLI 307
L+LM G +G ++P W MH +C W+L+
Sbjct: 825 LELMIGFPIGVRTSSPTWQMHL---WLCPWLLL 914
>TC210299 homologue to UP|EFT2_SOYBN (P46280) Elongation factor Tu,
chloroplast precursor (EF-Tu), complete
Length = 1615
Score = 29.3 bits (64), Expect = 2.7
Identities = 17/62 (27%), Positives = 30/62 (47%)
Frame = +3
Query: 10 YKLHTSTFERAYPRSHGLLKSGKPFQSRRFNFPSIRLNQSFICCTKLTPWEPSPGVAYAP 69
+KL T + +++GL+K ++ FPS+ +F C L + PSP V +P
Sbjct: 747 WKL*WPTLPSSAAKTNGLIKFTSSWKLWMTTFPSLSAKLNFPFCWPLRMFSPSPVVEPSP 926
Query: 70 TD 71
+
Sbjct: 927 PE 932
>TC217356 similar to UP|Q84JM5 (Q84JM5) Angustifolia, partial (57%)
Length = 1672
Score = 25.8 bits (55), Expect(2) = 3.1
Identities = 17/54 (31%), Positives = 25/54 (45%)
Frame = -1
Query: 48 QSFICCTKLTPWEPSPGVAYAPTDNQSDNFLQSTASVFETLESSKVDESPTANV 101
QS ++TP P+ T +D FLQ T S+ E S+V S T+ +
Sbjct: 1393 QSLYSSQRVTPSSPTVAKISTRTSKTADGFLQFT-SLHSCKEPSRVQFSQTSKL 1235
Score = 21.6 bits (44), Expect(2) = 3.1
Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 4/42 (9%)
Frame = -3
Query: 9 HYK----LHTSTFERAYPRSHGLLKSGKPFQSRRFNFPSIRL 46
HY+ H TFER+ G++ PF+ R P+I L
Sbjct: 1499 HYEYIPIFHKETFERSLSHGTGVIYKLLPFEYYR---PTITL 1383
>AI442632
Length = 322
Score = 28.9 bits (63), Expect = 3.6
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = +3
Query: 114 ELQLFKWPMWLLGPSILLATGMVPTLWLPI 143
+L L WP+ PS+L++ +P+ W+P+
Sbjct: 207 QLLLLDWPLEATSPSLLVSYTGLPSCWVPL 296
>TC224890 similar to UP|TIP2_TOBAC (P24422) Probable aquaporin TIP-type
RB7-18C (Tonoplast intrinsic protein, root-specific
RB7-18C) (TobRB7) (RT-TIP), partial (71%)
Length = 778
Score = 28.9 bits (63), Expect = 3.6
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = +2
Query: 114 ELQLFKWPMWLLGPSILLATGMVPTLWLPI 143
+L L WP+ PS+L++ +P+ W+P+
Sbjct: 254 QLLLLDWPLEATSPSLLVSYTGLPSCWVPL 343
>TC224822 similar to UP|TIP2_TOBAC (P24422) Probable aquaporin TIP-type
RB7-18C (Tonoplast intrinsic protein, root-specific
RB7-18C) (TobRB7) (RT-TIP), partial (96%)
Length = 1202
Score = 28.9 bits (63), Expect = 3.6
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = +2
Query: 114 ELQLFKWPMWLLGPSILLATGMVPTLWLPI 143
+L L WP+ PS+L++ +P+ W+P+
Sbjct: 350 QLLLLDWPLEATSPSLLVSYTGLPSCWVPL 439
>TC208709 similar to UP|Q8S9G8 (Q8S9G8) AT5g18830/F17K4_80, partial (18%)
Length = 1680
Score = 28.5 bits (62), Expect = 4.6
Identities = 26/95 (27%), Positives = 42/95 (43%)
Frame = -3
Query: 183 KNPTQEIKSKAPFSYQFWNIVATLTGFIVPSLLMFGSQKGFLQPQLPFISSAVLLGPYLL 242
KNP K + P+ +I+ ++ F L +F + G P SSA+ P+LL
Sbjct: 1306 KNPLP--KHRRPYLSGNSSILIIISLFKSGILSIFSLEVGLFTFSTPHFSSALDWIPWLL 1133
Query: 243 LLSVQILTELLTWYWQSPVWLVTPIIYEAYRILQL 277
+ + +L+ W+ P + P I E IL L
Sbjct: 1132 IATDSLLS------WEQP*ASIKPFISECTIILSL 1046
>NP004888 putative cytochrome P450
Length = 1500
Score = 28.5 bits (62), Expect = 4.6
Identities = 18/36 (50%), Positives = 23/36 (63%), Gaps = 3/36 (8%)
Frame = -2
Query: 134 GMVPTLW-LPISSIFLGPNIASLL-SLIG-LDCIFN 166
G VP+ W LP+ FLGP +SLL S +G L C+ N
Sbjct: 158 GNVPSWWRLPMKGSFLGPGGSSLLFSAVGFLSCLTN 51
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.137 0.430
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,144,262
Number of Sequences: 63676
Number of extensions: 293058
Number of successful extensions: 1874
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 1852
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1872
length of query: 344
length of database: 12,639,632
effective HSP length: 98
effective length of query: 246
effective length of database: 6,399,384
effective search space: 1574248464
effective search space used: 1574248464
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148718.5