BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148718.4 + phase: 0
(324 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC228780 334 4e-92
TC228781 126 1e-29
TC220735 87 1e-17
TC207574 weakly similar to UP|Q8W228 (Q8W228) Cytochrome P450, p... 33 0.23
AW460156 similar to GP|18033922|gb SEUSS transcriptional co-regu... 32 0.39
CF922863 31 0.87
AW755271 30 1.1
TC234601 similar to UP|MT70_ARATH (O82486) Probable N6-adenosine... 30 1.5
TC232949 homologue to UP|Q9FIR6 (Q9FIR6) Gb|AAD25674.1, partial ... 30 1.9
CO036556 30 1.9
BG156250 30 1.9
TC206788 similar to UP|Q940Y6 (Q940Y6) AT4g27600/T29A15_90, part... 29 2.5
TC205920 similar to UP|14P_ARATH (O64628) UPF0071 protein At2g18... 29 2.5
BM892194 similar to GP|6524879|gb|A cytochrome b {Tachyoryctes s... 29 3.3
TC232940 homologue to UP|Q7XVJ5 (Q7XVJ5) OJ000126_13.10 protein,... 28 4.3
TC214026 similar to UP|Q6G0R7 (Q6G0R7) GTP-binding protein typA,... 28 4.3
TC223603 weakly similar to UP|Q9ZQ26 (Q9ZQ26) Expressed protein ... 28 5.6
TC224872 homologue to UP|Q93VR3 (Q93VR3) AT5g28840/F7P1_20, part... 28 5.6
TC214015 28 5.6
BM891699 28 7.4
>TC228780
Length = 1076
Score = 334 bits (856), Expect = 4e-92
Identities = 157/197 (79%), Positives = 172/197 (86%)
Frame = +1
Query: 128 LNLKGDDGFRMNEVLVSEWYIMGFWPLVYSMLLLPTGRSSKSSVPVWPFLSASFFGGIYA 187
LNLKGDDGFRMNEVLVS WY+MG WPLVYSMLLLPTGRSSK+ +PVWPFL S FGG YA
Sbjct: 1 LNLKGDDGFRMNEVLVSLWYLMGLWPLVYSMLLLPTGRSSKNIIPVWPFLILSCFGGAYA 180
Query: 188 LLPYFVLWKPPPPPVEEAELKTWPLNFLESKITAMILLASGIGIIIYAGLAGEDVWKEFF 247
L PYFVLWKPP PPVEE +LKTWPLNFLESK+TA I LA+G+ II YAGLAG+DVWKEF+
Sbjct: 181 LFPYFVLWKPPAPPVEETQLKTWPLNFLESKVTATISLAAGVAIITYAGLAGQDVWKEFY 360
Query: 248 QYCRESKFIHITSIDFTLLSTLAPFWVYNDMTARKWFDKGSWLLPVSLIPLLGPALYILL 307
QY RESKFIHI SIDF +LST APFWVYNDMTARK DKGSWLLP+SLIP LGP LY+LL
Sbjct: 361 QYFRESKFIHIMSIDFIILSTFAPFWVYNDMTARKRSDKGSWLLPLSLIPFLGPGLYLLL 540
Query: 308 RPSLSTSAVAQSPAESE 324
RPSLST A++Q+P E E
Sbjct: 541 RPSLSTVAISQTPVEPE 591
>TC228781
Length = 408
Score = 126 bits (317), Expect = 1e-29
Identities = 59/73 (80%), Positives = 65/73 (88%)
Frame = +2
Query: 252 ESKFIHITSIDFTLLSTLAPFWVYNDMTARKWFDKGSWLLPVSLIPLLGPALYILLRPSL 311
ESKFIHITSIDF +LST APFWVYNDMTARK FDKGSWLLP+SLIP LGP LY+LLRPSL
Sbjct: 2 ESKFIHITSIDFIILSTFAPFWVYNDMTARKRFDKGSWLLPISLIPFLGPGLYLLLRPSL 181
Query: 312 STSAVAQSPAESE 324
ST A++Q+P E E
Sbjct: 182 STVAISQTPVEPE 220
>TC220735
Length = 456
Score = 87.0 bits (214), Expect = 1e-17
Identities = 58/140 (41%), Positives = 77/140 (54%), Gaps = 15/140 (10%)
Frame = +1
Query: 1 MVLVVAKPNLISCNFPSLKTKKS--------------IQPHITVVASGCCCRLSSFKPL- 45
+ +++A+P +S NFP+L + S + P T + + C KPL
Sbjct: 55 LTMLLAQPIFLSRNFPTLNNRNSRTSLQLHNPPALIRVSPQRTPIIASSGCFGCFVKPLD 234
Query: 46 VAMKTPFVAATTSFRRKSSSSSIVICNDSNKQNSSVEEKEEVKRDWTTSILLFLLWAALI 105
V KT A F+++ + I C+ S K+ S V RDWTTSILLFLLWAAL+
Sbjct: 235 VRTKTSLGAF---FKKRKGCAHI--CH-SLKEESEVGGNGNNGRDWTTSILLFLLWAALM 396
Query: 106 YYVSFLSPNQTPSRDMYFLK 125
YYV FL+PNQTPSRD YFLK
Sbjct: 397 YYVFFLTPNQTPSRDTYFLK 456
>TC207574 weakly similar to UP|Q8W228 (Q8W228) Cytochrome P450, partial (33%)
Length = 1172
Score = 32.7 bits (73), Expect = 0.23
Identities = 21/61 (34%), Positives = 31/61 (50%), Gaps = 2/61 (3%)
Frame = -3
Query: 169 SSVPVWPFLSASFFGGIYALLPYFVLWKP--PPPPVEEAELKTWPLNFLESKITAMILLA 226
SS P LS + +A +P++ L P PP P +L PLNF + +T IL++
Sbjct: 627 SSSPGEQVLSIRIYDP*HAPIPFYTLCSP*APPTPH*GPQLHVMPLNFSVTSLTLPILVS 448
Query: 227 S 227
S
Sbjct: 447 S 445
>AW460156 similar to GP|18033922|gb SEUSS transcriptional co-regulator
{Arabidopsis thaliana}, partial (12%)
Length = 429
Score = 32.0 bits (71), Expect = 0.39
Identities = 14/36 (38%), Positives = 19/36 (51%)
Frame = +2
Query: 23 SIQPHITVVASGCCCRLSSFKPLVAMKTPFVAATTS 58
++ PH+ + SGCCC SF P A F TT+
Sbjct: 182 TVSPHVKSIVSGCCCPDQSFAPSPA----FTVTTTT 277
>CF922863
Length = 392
Score = 30.8 bits (68), Expect = 0.87
Identities = 18/57 (31%), Positives = 25/57 (43%), Gaps = 3/57 (5%)
Frame = -3
Query: 146 WYIM---GFWPLVYSMLLLPTGRSSKSSVPVWPFLSASFFGGIYALLPYFVLWKPPP 199
W+++ GF+P + +L G W FL FFGG +A WK PP
Sbjct: 180 WFLLCPWGFFPFFFGFKMLHLG---------WIFLGLFFFGGSFA-------WKAPP 58
>AW755271
Length = 425
Score = 30.4 bits (67), Expect = 1.1
Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 7/42 (16%)
Frame = +2
Query: 197 PPPPPVEEAELKTWPLN-------FLESKITAMILLASGIGI 231
PPPPP EE ++KT N ++ + +LL +GIGI
Sbjct: 275 PPPPPTEETDVKTQEDNQEAEKAPEVDQPSNSFLLLLNGIGI 400
>TC234601 similar to UP|MT70_ARATH (O82486) Probable
N6-adenosine-methyltransferase MT-A70-like protein ,
partial (14%)
Length = 804
Score = 30.0 bits (66), Expect = 1.5
Identities = 20/58 (34%), Positives = 27/58 (46%), Gaps = 1/58 (1%)
Frame = -3
Query: 184 GIYALLPYFVLWKPPPPPVEEAELKTWPLNFLESKITAMILLASGI-GIIIYAGLAGE 240
G+YAL Y L+ PP P+ W L + K A +L G+ G+ Y LA E
Sbjct: 661 GVYALKEYLYLYVPPSCPL------VWVLPLVLLKTLAAMLTYGGLHGVSTYQTLASE 506
>TC232949 homologue to UP|Q9FIR6 (Q9FIR6) Gb|AAD25674.1, partial (20%)
Length = 647
Score = 29.6 bits (65), Expect = 1.9
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = -3
Query: 37 CRLSSFKPLVAMKTPFVAATTSFRRKSSSSSIVICNDSNKQNSSVEEK 84
CR + K ++ F A+T+FR SSS SI + N SS K
Sbjct: 516 CRSPAGKSIIVFCHSFNTASTNFRSNSSSKSIRLLNKPPSSPSSPSSK 373
>CO036556
Length = 545
Score = 29.6 bits (65), Expect = 1.9
Identities = 16/54 (29%), Positives = 25/54 (45%), Gaps = 1/54 (1%)
Frame = +3
Query: 17 SLKTKKSIQPHITVVASGCCCR-LSSFKPLVAMKTPFVAATTSFRRKSSSSSIV 69
S T + PH+ + SGCCC SSF +A T+ + ++S+ V
Sbjct: 33 STTTTATTAPHVKAILSGCCCSDESSFAAAETFAISTTSAATAATPEDNASTTV 194
>BG156250
Length = 426
Score = 29.6 bits (65), Expect = 1.9
Identities = 21/62 (33%), Positives = 29/62 (45%)
Frame = -3
Query: 239 GEDVWKEFFQYCRESKFIHITSIDFTLLSTLAPFWVYNDMTARKWFDKGSWLLPVSLIPL 298
G +W EF + +E + IH +DF S A + TAR+W K WL + I
Sbjct: 229 GSILWWEFLSWVKEVRTIHCRPMDFLQHSPTAG----SSATARRW--KLWWLAATNEIWK 68
Query: 299 LG 300
LG
Sbjct: 67 LG 62
>TC206788 similar to UP|Q940Y6 (Q940Y6) AT4g27600/T29A15_90, partial (58%)
Length = 1182
Score = 29.3 bits (64), Expect = 2.5
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = -2
Query: 14 NFPSLKTKKSIQPHITVVASGCCCRLSSFKPLVAMKTPFVAA 55
+FP KTK + P I+ +S C + + ++A K PFV A
Sbjct: 1058 SFPFAKTKSTQFPIISQKSSKCLSMQETSEAVIATKAPFVRA 933
>TC205920 similar to UP|14P_ARATH (O64628) UPF0071 protein At2g18990, partial
(86%)
Length = 985
Score = 29.3 bits (64), Expect = 2.5
Identities = 16/45 (35%), Positives = 23/45 (50%), Gaps = 3/45 (6%)
Frame = -2
Query: 279 TARKWFDKGSWLLPVSLIPLLGPALYI---LLRPSLSTSAVAQSP 320
T R WF SW+LP+S+ P ++ +R LS+ V SP
Sbjct: 171 TVRTWFSITSWILPLSIFRYQMPLKWMNNESIRRRLSSPLVPSSP 37
>BM892194 similar to GP|6524879|gb|A cytochrome b {Tachyoryctes splendens},
partial (6%)
Length = 420
Score = 28.9 bits (63), Expect = 3.3
Identities = 18/53 (33%), Positives = 29/53 (53%), Gaps = 12/53 (22%)
Frame = +1
Query: 154 LVYSM-LLLPTGRSSKSSVPVWPFLSASF-----------FGGIYALLPYFVL 194
+ YS+ ++L +G+ K S P++ + S SF FGGI+ L P+F L
Sbjct: 25 IYYSLFVILSSGKPFKYSFPIYIYHSFSFITLSNLVALSPFGGIHFLFPFFSL 183
>TC232940 homologue to UP|Q7XVJ5 (Q7XVJ5) OJ000126_13.10 protein, partial
(13%)
Length = 796
Score = 28.5 bits (62), Expect = 4.3
Identities = 21/63 (33%), Positives = 30/63 (47%), Gaps = 8/63 (12%)
Frame = +3
Query: 39 LSSFKPLVAMKTPFVAATTSFRRKSSSSSI--------VICNDSNKQNSSVEEKEEVKRD 90
+SSF L + + + T SF R+SSSSS + S+ +SS EE E +
Sbjct: 36 ISSFLSLHSQQNKSMDPTPSFHRRSSSSSSDRLLGPYPFSPSPSSAASSSAEELNEAELF 215
Query: 91 WTT 93
W T
Sbjct: 216WAT 224
>TC214026 similar to UP|Q6G0R7 (Q6G0R7) GTP-binding protein typA, partial
(14%)
Length = 501
Score = 28.5 bits (62), Expect = 4.3
Identities = 21/58 (36%), Positives = 24/58 (41%)
Frame = +2
Query: 154 LVYSMLLLPTGRSSKSSVPVWPFLSASFFGGIYALLPYFVLWKPPPPPVEEAELKTWP 211
L +LL PT SS + P P L LP +PPPPP A TWP
Sbjct: 122 LFLPLLLYPTRASSHAPSPPLPPLP----------LPQ----QPPPPPSTPAASATWP 253
>TC223603 weakly similar to UP|Q9ZQ26 (Q9ZQ26) Expressed protein
(At2g24420/T28I24.15), partial (8%)
Length = 462
Score = 28.1 bits (61), Expect = 5.6
Identities = 12/52 (23%), Positives = 27/52 (51%)
Frame = -3
Query: 243 WKEFFQYCRESKFIHITSIDFTLLSTLAPFWVYNDMTARKWFDKGSWLLPVS 294
W+E +C++ I ++S+DF+ + ++ F+ + D W+L +S
Sbjct: 328 WREAISFCKDCMVIDLSSMDFSFSAIISSFF-FTSSNFSLI*DSRIWILELS 176
>TC224872 homologue to UP|Q93VR3 (Q93VR3) AT5g28840/F7P1_20, partial (98%)
Length = 1554
Score = 28.1 bits (61), Expect = 5.6
Identities = 17/52 (32%), Positives = 24/52 (45%), Gaps = 4/52 (7%)
Frame = -3
Query: 177 LSASFFGGIYALLPYFVL----WKPPPPPVEEAELKTWPLNFLESKITAMIL 224
L+AS + +L Y + W P PP+ A LKTW + K +M L
Sbjct: 580 LAASIMLKLIMVLLYIMTEWLDWIKPIPPISAARLKTWSTPLVTVKQLSMTL 425
>TC214015
Length = 536
Score = 28.1 bits (61), Expect = 5.6
Identities = 21/61 (34%), Positives = 32/61 (52%), Gaps = 3/61 (4%)
Frame = +1
Query: 146 WYIMGFWPLVY-SMLLLPTGRSSKSSVPVWPF--LSASFFGGIYALLPYFVLWKPPPPPV 202
++I F P V S+ L PT S++P +PF L SFF ++ +P F + PPP
Sbjct: 31 FFIFLFGPTVIPSLSLEPT-----STLPTFPFNSLHNSFFPNTHSHIPKFPPFSLQPPPT 195
Query: 203 E 203
+
Sbjct: 196 K 198
>BM891699
Length = 421
Score = 27.7 bits (60), Expect = 7.4
Identities = 16/38 (42%), Positives = 21/38 (55%), Gaps = 4/38 (10%)
Frame = +3
Query: 41 SFKPLVAMKTPFVAATTSFRRK----SSSSSIVICNDS 74
SF P ++T TT+FRR+ SSS S+V C S
Sbjct: 288 SFSPESNLRTAVPLLTTTFRRRVLFTSSSVSVVACRSS 401
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.137 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,189,684
Number of Sequences: 63676
Number of extensions: 328951
Number of successful extensions: 2389
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 2312
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2380
length of query: 324
length of database: 12,639,632
effective HSP length: 97
effective length of query: 227
effective length of database: 6,463,060
effective search space: 1467114620
effective search space used: 1467114620
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148718.4