BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148227.2 + phase: 0 /pseudo
(1075 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC235104 weakly similar to UP|Q850H8 (Q850H8) Gag-pol polyprotei... 91 2e-18
CO981879 68 1e-11
TC204439 UP|Q84VI4 (Q84VI4) Gag-pol polyprotein, complete 61 2e-09
BI427153 61 3e-09
CO983154 60 5e-09
TC204438 homologue to UP|Q84VH6 (Q84VH6) Gag-pol polyprotein, co... 59 1e-08
TC222253 similar to UP|Q9ZQE4 (Q9ZQE4) Copia-like retroelement p... 54 3e-07
TC233180 similar to UP|Q944K0 (Q944K0) At1g18030/T10F20_3, parti... 54 4e-07
CD411510 44 5e-06
TC233822 46 1e-04
TC223332 45 1e-04
CO985828 42 0.001
BI701169 40 0.004
BI425121 40 0.006
BI424202 40 0.007
TC213920 39 0.016
CD412284 similar to GP|21434|emb|CA ORF4 {Solanum tuberosum}, pa... 38 0.021
AW666085 similar to GP|21434|emb|CA ORF4 {Solanum tuberosum}, pa... 38 0.021
TC207859 37 0.036
TC213393 weakly similar to UP|Q850H8 (Q850H8) Gag-pol polyprotei... 32 0.073
>TC235104 weakly similar to UP|Q850H8 (Q850H8) Gag-pol polyprotein
(Fragment), partial (28%)
Length = 865
Score = 91.3 bits (225), Expect = 2e-18
Identities = 81/270 (30%), Positives = 112/270 (41%), Gaps = 18/270 (6%)
Frame = +2
Query: 2 NLLSVSKLIWDKRCQTHFFDTHCLFQDSISGKTIVSAKKNGGLYYLEDELETGHQLGQIS 61
N+ S+S+L + C F + Q+ +G TI ++ GLYYL+ L
Sbjct: 128 NITSLSQLTRFRNCSVTFDANSFVIQECGTGWTIGVGIESHGLYYLKPNLS--------- 280
Query: 62 SFSESFFVSNNKDDVMLWHLRLGHPSFKYLKIVFPKLFFGHDFSSFQCEIYEFSKQHRSS 121
+V + L H RLGHP LKI+ P L CE + K RSS
Sbjct: 281 ------WVCSAVTSPKLLHERLGHPHLSKLKIMVPSL---EKIKDLFCESCQLGKHVRSS 433
Query: 122 FPVQTYKPSKLFSIIHSDVWGPNRINSYPTKDGLSPLLMIILDFIGYIY*KKNQR*DKLS 181
+ F +IH D+WGPNR++S M F+ +I + +
Sbjct: 434 XRHVESRVDSPFLVIHXDIWGPNRVSS-----------MSYRYFVTFI-----DEFSQCT 565
Query: 182 RILLN*CRLSLIPLYKFSELTMELNILTQF*ETF------------------FFMENGIV 223
R+ L R ++ S LT I TQF +T F GI+
Sbjct: 566 RVFLMKERSEIL-----SFLTSVNKIKTQFGKTIKILRSDNAKEYFSSVISPFXSAQGIL 730
Query: 224 QQSTCVSSPQQNGITERKNRHLLEMARALL 253
Q +C +PQQN I ERKNRHL+E AR LL
Sbjct: 731 HQFSCPHTPQQNDIAERKNRHLVETARTLL 820
>CO981879
Length = 576
Score = 68.2 bits (165), Expect(2) = 1e-11
Identities = 54/152 (35%), Positives = 73/152 (47%), Gaps = 1/152 (0%)
Frame = -1
Query: 218 MENGIVQQSTCVSSPQQNGITERKNRHLLEMARALLFFH*SSKILMG*GCINCCTLNKLY 277
+ENGI+ QS+CV +PQQNG+ ERKNRHL E+ARALLF + + K G + L
Sbjct: 459 LENGIIHQSSCVDTPQQNGVAERKNRHLXEVARALLFQNKAPKYXWGEAILTGTYLKNKN 280
Query: 278 VISCFKP*DSSRNLLKILSKCPYFSRFAFKNIRMHYFCP*TQTN-RQT*TRASKRVFVGY 336
+ +S R K SK NI +H FC + T R+T* + K F
Sbjct: 279 A*QNLEFQNSIRCFHKCFSK*QALVYSTS*NIWVHCFCTYS*TKPRKT*A*SKKMCFCWL 100
Query: 337 SPTRKGYKCLDLNSKRFLVTMDVTFF*K*TFF 368
KG + + + L F*K +FF
Sbjct: 99 CSQPKGIQMF*SHFQENLCYY*CYLF*KNSFF 4
Score = 20.8 bits (42), Expect(2) = 1e-11
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -2
Query: 197 KFSELTMELNILTQF*ETFF 216
KF +TME NILT * + F
Sbjct: 518 KFFVVTMEGNILTST*ASXF 459
>TC204439 UP|Q84VI4 (Q84VI4) Gag-pol polyprotein, complete
Length = 4731
Score = 61.2 bits (147), Expect = 2e-09
Identities = 66/276 (23%), Positives = 112/276 (39%), Gaps = 24/276 (8%)
Frame = +1
Query: 2 NLLSVSKLIWDKRCQTHFFDTHCLFQDSISGKTIVSAKKNGGLYYLEDELETGHQLGQIS 61
NL+S+S+L D+ +F + CL + S + ++ ++ YL ET +
Sbjct: 1876 NLISISQLC-DEGFNVNFTKSECLVTNEKS-EVLMKGSRSKDNCYLWTPQETSYS----- 2034
Query: 62 SFSESFFVSNNKDDVMLWHLRLGHPSFKYLKIV--------FPKLFFGHDFSSFQCEIYE 113
S +S+ +D+V +WH R GH + +K + P L +C+I +
Sbjct: 2035 ----STCLSSKEDEVRIWHQRFGHLHLRGMKKIIDKGAVRGIPNLKIEEGRICGECQIGK 2202
Query: 114 FSKQHRSSFPVQTYKPSKLFSIIHSDVWGPNRINSYPTKDGLSPLLMIILDFIGYIY*KK 173
K QT S++ ++H D+ GP ++ S G +++ DF + +
Sbjct: 2203 QVKMSHQKLQHQT--TSRVLELLHMDLMGPMQVESL---GGKRYAYVVVDDFSRFTW--- 2358
Query: 174 NQR*DKLSRILLN*CRLSLIPLYKFSELTMELN-----ILTQF*E-----------TFFF 217
+N R F EL++ L ++ + T F
Sbjct: 2359 -----------VNFIREKSETFEVFKELSLRLQREKDCVIKRIRSDHGREFENSRFTEFC 2505
Query: 218 MENGIVQQSTCVSSPQQNGITERKNRHLLEMARALL 253
GI + + +PQQNGI ERKNR L E AR +L
Sbjct: 2506 TSEGITHEFSAAITPQQNGIVERKNRTLQEAARVML 2613
>BI427153
Length = 422
Score = 60.8 bits (146), Expect = 3e-09
Identities = 51/150 (34%), Positives = 69/150 (46%), Gaps = 12/150 (8%)
Frame = +1
Query: 225 QSTCVSSPQQNGITERKNRHLLEMARALLF------FH*SSKILMG*GCINCCTLNKLYV 278
QSTC +PQQNGI ERKN HLLE AR+L+ H +L C +N++
Sbjct: 4 QSTCPHTPQQNGIAERKNHHLLETARSLMLNSNVPTHHWGDAVLTA-----CFLINRM-- 162
Query: 279 ISCFKP*DSSRNLLKILSKCP-----YFSRFAFK-NIRMHYFCP*TQTNRQT*TRASKRV 332
P S N + P Y S F +H P + R+ K V
Sbjct: 163 -----PSSSLENQIPHSIVFPNDLLFYVSPKVFGCTCFVHDLSPGLD---KLSARSVKCV 318
Query: 333 FVGYSPTRKGYKCLDLNSKRFLVTMDVTFF 362
F+GYS +KGY C N +R+ ++ +VTFF
Sbjct: 319 FLGYSRLQKGYTCYFPNMRRYYMSANVTFF 408
>CO983154
Length = 568
Score = 60.1 bits (144), Expect = 5e-09
Identities = 50/147 (34%), Positives = 67/147 (45%), Gaps = 9/147 (6%)
Frame = +3
Query: 231 SPQQNGITERKNRHLLEMARALLF------FH*SSKILMG*GCIN---CCTLNKLYVISC 281
+PQQNGI ERKNRHLLE AR+L+ H +L IN +L S
Sbjct: 6 TPQQNGIAERKNRHLLETARSLMLNLNVPIHHWGDAVLTSCFLINRMPSSSLENQIPHSL 185
Query: 282 FKP*DSSRNLLKILSKCPYFSRFAFKNIRMHYFCP*TQTNRQT*TRASKRVFVGYSPTRK 341
P D ++ + C F +H P + R+ K VF+GYS +K
Sbjct: 186 VFPHDPLFHVSPKVFGCTCF---------VHDLSPGLD---KLSARSVKCVFLGYSRLQK 329
Query: 342 GYKCLDLNSKRFLVTMDVTFF*K*TFF 368
GYKC +R+ ++ DVTFF FF
Sbjct: 330 GYKCYSPTMRRYYMSADVTFFEDTPFF 410
>TC204438 homologue to UP|Q84VH6 (Q84VH6) Gag-pol polyprotein, complete
Length = 4734
Score = 58.9 bits (141), Expect = 1e-08
Identities = 65/276 (23%), Positives = 111/276 (39%), Gaps = 24/276 (8%)
Frame = +1
Query: 2 NLLSVSKLIWDKRCQTHFFDTHCLFQDSISGKTIVSAKKNGGLYYLEDELETGHQLGQIS 61
NL+S+S+L D+ +F + CL + S + ++ ++ YL ET +
Sbjct: 1879 NLISISQLC-DEGFNVNFTKSECLVTNEKS-EVLMKGSRSKDNCYLWTPQETSYS----- 2037
Query: 62 SFSESFFVSNNKDDVMLWHLRLGHPSFKYLKIV--------FPKLFFGHDFSSFQCEIYE 113
S + + +D+V +WH R GH + +K + P L +C+I +
Sbjct: 2038 ----STCLFSKEDEVKIWHQRFGHLHLRGMKKIIDKGAVRGIPNLKIEEGRICGECQIGK 2205
Query: 114 FSKQHRSSFPVQTYKPSKLFSIIHSDVWGPNRINSYPTKDGLSPLLMIILDFIGYIY*KK 173
K QT S++ ++H D+ GP ++ S G +++ DF + +
Sbjct: 2206 QVKMSHQKLQHQT--TSRVLELLHMDLMGPMQVESL---GGKRYAYVVVDDFSRFTW--- 2361
Query: 174 NQR*DKLSRILLN*CRLSLIPLYKFSELTMELN-----ILTQF*E-----------TFFF 217
+N R F EL++ L ++ + T F
Sbjct: 2362 -----------VNFIREKSDTFEVFKELSLRLQREKDCVIKRIRSDHGREFENSKFTEFC 2508
Query: 218 MENGIVQQSTCVSSPQQNGITERKNRHLLEMARALL 253
GI + + +PQQNGI ERKNR L E AR +L
Sbjct: 2509 TSEGITHEFSAAITPQQNGIVERKNRTLQEAARVML 2616
>TC222253 similar to UP|Q9ZQE4 (Q9ZQE4) Copia-like retroelement pol
polyprotein, partial (4%)
Length = 919
Score = 54.3 bits (129), Expect = 3e-07
Identities = 43/155 (27%), Positives = 70/155 (44%), Gaps = 10/155 (6%)
Frame = +1
Query: 109 CEIYEFSKQHRSSFPV-QTYKPSKLFSIIHSDVWGPNRINSYPTKDGLSPLLMIILDFIG 167
C+ E K+HR SFP ++++ KL I+H D+ PT + + I DF
Sbjct: 331 CDTCEIGKKHRESFPTGKSWRMKKLLKIVHLDLCTVE----IPTHGDNNYFITFIDDFSK 498
Query: 168 ----YIY*KKNQR*DKLSRILL-----N*CRLSLIPLYKFSELTMELNILTQF*ETFFFM 218
Y +K++ + N C++ + + K E T FF
Sbjct: 499 KMWVYFLKQKSEACNAFKMFKAFAEKQNGCKVKALIIDKGQEYLSY---------TIFFE 651
Query: 219 ENGIVQQSTCVSSPQQNGITERKNRHLLEMARALL 253
++GI Q T +PQ NG+TERKN+ +++M R +L
Sbjct: 652 KHGIQHQLTTKYTPQHNGVTERKNKTIMDMVRCML 756
>TC233180 similar to UP|Q944K0 (Q944K0) At1g18030/T10F20_3, partial (11%)
Length = 916
Score = 53.9 bits (128), Expect = 4e-07
Identities = 36/97 (37%), Positives = 48/97 (49%), Gaps = 10/97 (10%)
Frame = +2
Query: 2 NLLSVSKLIWDKRCQTHFFDTHCLFQDSISGKTIVSAKKNGGLYYLEDE----------L 51
NLLS+ K+ D C FF +HC+F D G+ I AK+ GGLYYL+ E L
Sbjct: 647 NLLSIHKIT*DLNCVVTFFHSHCVF*DLAMGRMIGIAKE*GGLYYLQHEDNKECTR*KAL 826
Query: 52 ETGHQLGQISSFSESFFVSNNKDDVMLWHLRLGHPSF 88
+ HQ + SE + + + L H LGHP F
Sbjct: 827 TSNHQ-----TSSEPW----SSSQIWLQHKCLGHPPF 910
>CD411510
Length = 633
Score = 43.5 bits (101), Expect(2) = 5e-06
Identities = 33/71 (46%), Positives = 40/71 (55%), Gaps = 3/71 (4%)
Frame = -1
Query: 27 QDSISGKTIVSAKKNGGLYYLEDE---LETGHQLGQISSFSESFFVSNNKDDVMLWHLRL 83
QD +GK I ++ GLY LE+ T QL +S SES S+NKD + L H L
Sbjct: 285 QDQGTGKMIGLVREQNGLYLLEEARGICSTKIQL-PLSLMSESL-PSHNKD-IWLCHYHL 115
Query: 84 GHPSFKYLKIV 94
GHPSF LKIV
Sbjct: 114 GHPSFNTLKIV 82
Score = 26.2 bits (56), Expect(2) = 5e-06
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -2
Query: 94 VFPKLFFGHDFSSFQCEIYEFS 115
+FP LF G D F C+ +EF+
Sbjct: 86 LFPSLF*GLDIGVFHCDDFEFA 21
>TC233822
Length = 632
Score = 45.8 bits (107), Expect = 1e-04
Identities = 46/149 (30%), Positives = 66/149 (43%), Gaps = 1/149 (0%)
Frame = +2
Query: 2 NLLSVSKLIWDKRCQTHFFDTHCLFQDSISGKTIVSAKKNGGLYYLEDELETGHQLGQIS 61
NL+S+S+L F + Q+ + I ++ GLYYLE IS
Sbjct: 230 NLVSLSQLTKALNFSITFDADSFVIQERDTSWLIGVEHESRGLYYLE-----------IS 376
Query: 62 SFSESFFVSNNKDDVMLWHLRLGHPSFKYLKIVFPKLFFGHDFSSFQCEIYEFSKQHRSS 121
S F + K L H LGHP LK+V P L + +CE + K H
Sbjct: 377 SSMSCFATPSPK----LLHNHLGHPHLLKLKMV-PSL---NKLQVLECESCQLGK-HVRF 529
Query: 122 FPVQTY-KPSKLFSIIHSDVWGPNRINSY 149
FP +T + S +FS I SD+W P+ + S+
Sbjct: 530 FPKRTETRCSSVFSTIPSDIWDPSCVTSF 616
>TC223332
Length = 427
Score = 45.4 bits (106), Expect = 1e-04
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = +2
Query: 327 RASKRVFVGYSPTRKGYKCLDLNSKRFLVTMDVTFF*K*TFFFRTIIFKGGNQM 380
RA K +F+ YS T+KGY C L + RF +++DVTFF FF +I K +Q+
Sbjct: 41 RAIKCIFLDYSCTQKGYWCYSLANHRFYMSVDVTFFEDTPFFNSSISSKSVSQL 202
>CO985828
Length = 825
Score = 42.0 bits (97), Expect = 0.001
Identities = 31/88 (35%), Positives = 40/88 (45%), Gaps = 4/88 (4%)
Frame = -2
Query: 28 DSISGKTIVSAKKNGGLYYL--EDELETGHQLGQISSFSESFFVSNNKDDVMLW--HLRL 83
D +G+ I AK+ GGLYYL ED E Q S+ S + LW + L
Sbjct: 248 DLATGRAIGIAKE*GGLYYLQHEDNKECTKQKALTSNHQTS-------SEPWLWLQYKHL 90
Query: 84 GHPSFKYLKIVFPKLFFGHDFSSFQCEI 111
GHP F LK +FP LF F ++
Sbjct: 89 GHPPFSVLKSLFPFLFTKRXXQVFSLDV 6
>BI701169
Length = 407
Score = 40.4 bits (93), Expect = 0.004
Identities = 18/35 (51%), Positives = 26/35 (73%)
Frame = +2
Query: 219 ENGIVQQSTCVSSPQQNGITERKNRHLLEMARALL 253
++GI + + SPQQNG+ ERKNR +L MAR++L
Sbjct: 302 DHGIRRPLMVLRSPQQNGVAERKNRTILNMARSML 406
>BI425121
Length = 412
Score = 40.0 bits (92), Expect = 0.006
Identities = 24/66 (36%), Positives = 37/66 (55%)
Frame = +3
Query: 189 RLSLIPLYKFSELTMELNILTQF*ETFFFMENGIVQQSTCVSSPQQNGITERKNRHLLEM 248
++ + ++ E+TMEL++ * F NGI + +PQ+N + ERKNR L E
Sbjct: 120 KMKKVFVFLLLEVTMELSLRILS*N-HFCERNGIFHNLS*PRTPQENRVVERKNRTLQEK 296
Query: 249 ARALLF 254
AR +LF
Sbjct: 297 ARTILF 314
>BI424202
Length = 421
Score = 39.7 bits (91), Expect = 0.007
Identities = 27/100 (27%), Positives = 46/100 (46%), Gaps = 1/100 (1%)
Frame = -3
Query: 72 NKDDVMLWHLRLGHPSFKYLK-IVFPKLFFGHDFSSFQCEIYEFSKQHRSSFPVQTYKPS 130
N++ MLWH RLGH S + +K +V + DF+ F+ + + + + S
Sbjct: 395 NEESSMLWHRRLGHISIERIKRLVNEGVLSTLDFADFETYVDCIKGKQTNKSKKGAKRSS 216
Query: 131 KLFSIIHSDVWGPNRINSYPTKDGLSPLLMIILDFIGYIY 170
L IIH+D+ P+ + L + I D+ Y+Y
Sbjct: 215 NLLEIIHTDICCPDM-----DANSLKYFITFIDDYSRYMY 111
>TC213920
Length = 428
Score = 38.5 bits (88), Expect = 0.016
Identities = 16/35 (45%), Positives = 26/35 (73%)
Frame = -3
Query: 219 ENGIVQQSTCVSSPQQNGITERKNRHLLEMARALL 253
E I Q T + +PQQNG++E++NR L++M R++L
Sbjct: 363 ETCICVQYTILGTPQQNGVSEKRNRTLMDMVRSML 259
>CD412284 similar to GP|21434|emb|CA ORF4 {Solanum tuberosum}, partial (13%)
Length = 539
Score = 38.1 bits (87), Expect = 0.021
Identities = 22/51 (43%), Positives = 32/51 (62%)
Frame = -1
Query: 992 TSIEIIL*QQGRNKYSS*SSST*SDEAY*NQSSLYKREIRFWNTMSPFCAF 1042
+S EI+L* QG N+Y ST* ++A *N + +K E R+W + FC+F
Sbjct: 452 SSNEIVL**QGCNQYLPEPCST*PNQAC*N**TFHKGEGRYWPNLHAFCSF 300
>AW666085 similar to GP|21434|emb|CA ORF4 {Solanum tuberosum}, partial (16%)
Length = 193
Score = 38.1 bits (87), Expect = 0.021
Identities = 21/48 (43%), Positives = 30/48 (61%)
Frame = +1
Query: 923 CCLGRIHYR*KIDFGLLYLRVGKLGNMEK*EARCSC*KQCRG*VYSNG 970
C LGRI+Y +I+ LL++ +GKLG + + RC +QC *V NG
Sbjct: 7 CRLGRIYY*SEINIRLLHICLGKLGYLNEQITRCRGHEQCLC*VQGNG 150
>TC207859
Length = 751
Score = 37.4 bits (85), Expect = 0.036
Identities = 24/67 (35%), Positives = 32/67 (46%), Gaps = 2/67 (2%)
Frame = +1
Query: 78 LWHLRLGHPSFKYLKIVFPKLFFGHDFSSFQCEIYEFSKQ--HRSSFPVQTYKPSKLFSI 135
L H L HPS L ++ P + S QCE Y K H S V + S F++
Sbjct: 475 LAH*HLHHPSLNKLHLLVPSVSI---IKSLQCESYLLGKHVCHTCSPHVNKHVASP-FAL 642
Query: 136 IHSDVWG 142
+HSD+WG
Sbjct: 643 VHSDIWG 663
>TC213393 weakly similar to UP|Q850H8 (Q850H8) Gag-pol polyprotein
(Fragment), partial (23%)
Length = 678
Score = 32.0 bits (71), Expect(2) = 0.073
Identities = 15/38 (39%), Positives = 21/38 (54%), Gaps = 1/38 (2%)
Frame = -2
Query: 106 SFQCEIYEFSKQHRSSFPVQTYKPSKL-FSIIHSDVWG 142
+ CE +F K +SSFP + F ++HSDVWG
Sbjct: 356 TLSCESCQFGKHVQSSFPYCVICRDRFPFVLVHSDVWG 243
Score = 23.1 bits (48), Expect(2) = 0.073
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = -3
Query: 151 TKDGLSPLLMIILDFIGY 168
++D LSPL IILD +GY
Sbjct: 217 SQDILSPL*KIILDRLGY 164
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.355 0.158 0.554
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,415,527
Number of Sequences: 63676
Number of extensions: 770131
Number of successful extensions: 8966
Number of sequences better than 10.0: 62
Number of HSP's better than 10.0 without gapping: 5038
Number of HSP's successfully gapped in prelim test: 340
Number of HSP's that attempted gapping in prelim test: 3760
Number of HSP's gapped (non-prelim): 5687
length of query: 1075
length of database: 12,639,632
effective HSP length: 107
effective length of query: 968
effective length of database: 5,826,300
effective search space: 5639858400
effective search space used: 5639858400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.6 bits)
S2: 64 (29.3 bits)
Medicago: description of AC148227.2