BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148218.5 - phase: 0
(164 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW760164 similar to GP|11994422|dbj oxidoreductase short-chain ... 85 1e-17
CA937893 similar to GP|20805072|dbj retrovirus-related pol polyp... 54 4e-08
CF922226 51 3e-07
TC218798 similar to UP|Q8W4N9 (Q8W4N9) Nitrate transporter (NTL1... 29 1.2
TC230168 28 2.0
TC208708 weakly similar to UP|Q9SXD6 (Q9SXD6) T3P18.17, partial ... 28 2.0
BM143748 27 4.4
TC233837 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, part... 27 5.7
TC232792 26 7.5
TC205965 26 7.5
>AW760164 similar to GP|11994422|dbj oxidoreductase short-chain
dehydrogenase/reductase family-like protein {Arabidopsis
thaliana}, partial (9%)
Length = 428
Score = 85.1 bits (209), Expect = 1e-17
Identities = 45/91 (49%), Positives = 62/91 (67%), Gaps = 1/91 (1%)
Frame = +3
Query: 1 MGS-KWDIEKFTGDNDFRLWKVKMEAVLIQQKCEKTLKGESSLPVTMSRAEKTEMVDKAR 59
MGS K+++EKFTG NDF L +KM A+L+QQ + L GE L M+ +K ++ KA
Sbjct: 75 MGSAKYEVEKFTGQNDFGLC*LKMRALLVQQGLVEALDGEIKLEKMMADGDKKALLQKAY 254
Query: 60 SAIVLCLGNKDLREATKETTATSMWSKFEYL 90
+AI+L LG+K LR+ +KETTA +WSK E L
Sbjct: 255 NAIILSLGDKVLRQVSKETTAVGVWSKLEVL 347
>CA937893 similar to GP|20805072|dbj retrovirus-related pol polyprotein from
transposon TNT 1-94-like, partial (7%)
Length = 412
Score = 53.5 bits (127), Expect = 4e-08
Identities = 32/106 (30%), Positives = 55/106 (51%)
Frame = -2
Query: 2 GSKWDIEKFTGDNDFRLWKVKMEAVLIQQKCEKTLKGESSLPVTMSRAEKTEMVDKARSA 61
G+K+++ KF G +FRLW+ +++ +L Q K L+ S + E+ ++ +
Sbjct: 315 GAKFEVGKFDGTGNFRLWQKRVKDLLA*QGLLKVLRDSKSN--NTEALDWEEL*ERTATT 142
Query: 62 IVLCLGNKDLREATKETTATSMWSKFEYLYMTKSLAHRQVLKQQLY 107
I LCL ++ L + +W K E YM KSL ++ L Q+LY
Sbjct: 141 IRLCLVDEFLYHMMELAFPGEVWKKLESQYMLKSLTNKLYLMQKLY 4
>CF922226
Length = 667
Score = 50.8 bits (120), Expect = 3e-07
Identities = 30/60 (50%), Positives = 39/60 (65%), Gaps = 1/60 (1%)
Frame = -3
Query: 92 MTKSLAHRQVLKQQLYSFKMVKSKVVVELLVEFNKIIGDFENIKLHLEDAG-ALMVWCCL 150
MTKSL +R KQ LYSFKM + + V E L FNK+I D ENI + ++D AL++ C L
Sbjct: 665 MTKSLVNRLYXKQSLYSFKMHEDRSVGEQLDLFNKLILDLENIDVTIDDEDQALLLLCYL 486
>TC218798 similar to UP|Q8W4N9 (Q8W4N9) Nitrate transporter (NTL1), partial
(33%)
Length = 713
Score = 28.9 bits (63), Expect = 1.2
Identities = 18/49 (36%), Positives = 25/49 (50%)
Frame = +3
Query: 44 VTMSRAEKTEMVDKARSAIVLCLGNKDLREATKETTATSMWSKFEYLYM 92
V M+ A E V + R AI+ N L +ATK T W F+YL++
Sbjct: 192 VAMAVAAVVE-VKRKRVAIMATHSNSLLDDATKPLPITFFWIAFQYLFL 335
>TC230168
Length = 863
Score = 28.1 bits (61), Expect = 2.0
Identities = 13/42 (30%), Positives = 23/42 (53%)
Frame = -3
Query: 20 KVKMEAVLIQQKCEKTLKGESSLPVTMSRAEKTEMVDKARSA 61
++ AV+IQ C+++LKG +L T + TE + +A
Sbjct: 441 RMSQNAVMIQALCQRSLKGSVALKYTSYPIQATEEITVTANA 316
>TC208708 weakly similar to UP|Q9SXD6 (Q9SXD6) T3P18.17, partial (85%)
Length = 1011
Score = 28.1 bits (61), Expect = 2.0
Identities = 16/48 (33%), Positives = 22/48 (45%)
Frame = -1
Query: 37 KGESSLPVTMSRAEKTEMVDKARSAIVLCLGNKDLREATKETTATSMW 84
KG+ +LP+T + K KA A L AT +TAT+ W
Sbjct: 450 KGDLTLPLTPALLIKESTRPKASQAFCTALSMAGPSAATSSSTATARW 307
>BM143748
Length = 406
Score = 26.9 bits (58), Expect = 4.4
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = +2
Query: 5 WDIEKFTGDNDFRLWKVKMEAVLIQQKCEKTLK 37
W E F +LW+ K++ +QKC+ LK
Sbjct: 197 WFFEIFVTTTRLKLWRNKLQYQRSRQKCDHNLK 295
>TC233837 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, partial (6%)
Length = 402
Score = 26.6 bits (57), Expect = 5.7
Identities = 22/83 (26%), Positives = 36/83 (42%), Gaps = 16/83 (19%)
Frame = +2
Query: 72 REATKETTATSMWSKFEYLYMT--------------KSLAHRQVLKQ-QLYSFKMV-KSK 115
RE ++TT ++W F Y M +L H + K+ ++Y M+ KS+
Sbjct: 50 REDVEKTTFVTLWGTFSYRVMAFGLKNTGATYQRAMVALFHDMMHKEIEVYVDDMIAKSR 229
Query: 116 VVVELLVEFNKIIGDFENIKLHL 138
E LV K+ G + +L L
Sbjct: 230 TETEHLVNLCKLFGRLQKYQLKL 298
>TC232792
Length = 480
Score = 26.2 bits (56), Expect = 7.5
Identities = 14/46 (30%), Positives = 24/46 (51%)
Frame = -2
Query: 16 FRLWKVKMEAVLIQQKCEKTLKGESSLPVTMSRAEKTEMVDKARSA 61
F LW+V + V ++ +KG SSLP + S + M +++ A
Sbjct: 329 FFLWRVVVSTVRWRRFPSLRIKGTSSLPSSTSSSVAIRMQQRSKEA 192
>TC205965
Length = 1409
Score = 26.2 bits (56), Expect = 7.5
Identities = 13/40 (32%), Positives = 24/40 (59%)
Frame = +3
Query: 89 YLYMTKSLAHRQVLKQQLYSFKMVKSKVVVELLVEFNKII 128
YL++ KSL LK+ L+SF ++ ++ E L+E + +
Sbjct: 618 YLWLPKSLMVFAHLKELLFSFSDLELDIIKEDLLELSSTV 737
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.131 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,737,505
Number of Sequences: 63676
Number of extensions: 67298
Number of successful extensions: 323
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 323
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 323
length of query: 164
length of database: 12,639,632
effective HSP length: 90
effective length of query: 74
effective length of database: 6,908,792
effective search space: 511250608
effective search space used: 511250608
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC148218.5