BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147179.2 + phase: 0
(185 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC217186 similar to UP|Q8L7Z1 (Q8L7Z1) AT5g16250/T21H19_170, par... 263 3e-71
TC217187 similar to UP|Q8L7Z1 (Q8L7Z1) AT5g16250/T21H19_170, par... 188 1e-48
TC217185 120 5e-28
TC223452 similar to UP|Q8L7Z1 (Q8L7Z1) AT5g16250/T21H19_170, par... 75 2e-14
TC221779 32 0.17
CO985484 29 1.1
TC215931 similar to UP|O81618 (O81618) F8M12.11 protein, partial... 28 1.9
TC226446 homologue to UP|P2A_MEDSA (Q06009) Serine/threonine pro... 28 2.5
TC206933 similar to UP|Q99322 (Q99322) Mucin (Fragment), partial... 28 3.2
BI969502 27 5.5
TC215177 homologue to GB|AAP31934.1|30387537|BT006590 At2g35860 ... 27 5.5
TC234348 UP|Q79UP5 (Q79UP5) Blr1854 protein, partial (19%) 27 7.2
TC215865 homologue to UP|ARGI_SOYBN (O49046) Arginase , complete 27 7.2
TC232981 UP|Q9LLR0 (Q9LLR0) Carboxyl transferase alpha subunit ... 27 7.2
TC219056 similar to UP|Q9LVN1 (Q9LVN1) Gb|AAD23008.1, partial (7%) 27 7.2
TC225464 GB|AAB68399.1|1773321|HAU79736 calmodulin {Helianthus a... 26 9.4
TC205826 similar to UP|KELP_ARATH (O65155) RNA polymerase II tra... 26 9.4
TC214336 homologue to UP|Q8L4F2 (Q8L4F2) 40S ribosomal protein S... 26 9.4
TC206981 similar to UP|Q9LQB4 (Q9LQB4) F4N2.3, partial (25%) 26 9.4
>TC217186 similar to UP|Q8L7Z1 (Q8L7Z1) AT5g16250/T21H19_170, partial (74%)
Length = 534
Score = 263 bits (673), Expect = 3e-71
Identities = 127/146 (86%), Positives = 138/146 (93%)
Frame = +2
Query: 7 NPPATITPRHYNIHKLFLFCNYVLLGAASSCIFLTLSLRLVPSVCGFFLILLHIFTIAGA 66
N ++ITPRHYN HK+FLFCNY+LLGAASSCIFLTLSLRL+PS+CGFF ILL +FTIAGA
Sbjct: 95 NHNSSITPRHYNTHKVFLFCNYILLGAASSCIFLTLSLRLIPSLCGFFFILLQVFTIAGA 274
Query: 67 VSGCAAVGANRWYSAHMVATVLTAIFQGSVSVLVFTRTSDFLGELQSYVREEDGSVILKL 126
VSGCAAVG NRWYSAHMVATVLTAIFQGSVSVLVFTRT DFLG+L+SYVREEDG+VILKL
Sbjct: 275 VSGCAAVGTNRWYSAHMVATVLTAIFQGSVSVLVFTRTGDFLGQLKSYVREEDGAVILKL 454
Query: 127 SGGLAILIFCLEWVVLTLAFFLKYYA 152
+GGL ILIFCLEWVVLTLAFFLKYYA
Sbjct: 455 AGGLTILIFCLEWVVLTLAFFLKYYA 532
>TC217187 similar to UP|Q8L7Z1 (Q8L7Z1) AT5g16250/T21H19_170, partial (51%)
Length = 421
Score = 188 bits (477), Expect = 1e-48
Identities = 89/100 (89%), Positives = 96/100 (96%)
Frame = +2
Query: 11 TITPRHYNIHKLFLFCNYVLLGAASSCIFLTLSLRLVPSVCGFFLILLHIFTIAGAVSGC 70
+ITPRHYN HK+FLFCNY+LLGAASSCIFLTLSLRL+PS+CGFF ILL +FTIAGAVSGC
Sbjct: 122 SITPRHYNTHKVFLFCNYILLGAASSCIFLTLSLRLIPSLCGFFFILLQVFTIAGAVSGC 301
Query: 71 AAVGANRWYSAHMVATVLTAIFQGSVSVLVFTRTSDFLGE 110
AAVGANRWYSAHMVATVLTAIFQGSVSVLVFTRT DFLG+
Sbjct: 302 AAVGANRWYSAHMVATVLTAIFQGSVSVLVFTRTGDFLGQ 421
>TC217185
Length = 531
Score = 120 bits (300), Expect = 5e-28
Identities = 59/72 (81%), Positives = 63/72 (86%)
Frame = +3
Query: 114 YVREEDGSVILKLSGGLAILIFCLEWVVLTLAFFLKYYACVEGGNTCRTVVLGSAKVQQD 173
YVREEDG+VILKL+GGL ILIF LEWVVLTLAFFLKYYACVE GN+ V + S KVQQD
Sbjct: 3 YVREEDGAVILKLAGGLTILIFVLEWVVLTLAFFLKYYACVE-GNSGAVVPVRSGKVQQD 179
Query: 174 EDLKDWPWPFQV 185
EDLKDWPWPFQV
Sbjct: 180 EDLKDWPWPFQV 215
>TC223452 similar to UP|Q8L7Z1 (Q8L7Z1) AT5g16250/T21H19_170, partial (20%)
Length = 484
Score = 74.7 bits (182), Expect = 2e-14
Identities = 36/45 (80%), Positives = 38/45 (84%)
Frame = +2
Query: 141 VLTLAFFLKYYACVEGGNTCRTVVLGSAKVQQDEDLKDWPWPFQV 185
VLTLAFFLKYYACVEG N+ V + S KVQQDEDLKDWPWPFQV
Sbjct: 41 VLTLAFFLKYYACVEG-NSGAVVPVRSGKVQQDEDLKDWPWPFQV 172
>TC221779
Length = 475
Score = 32.0 bits (71), Expect = 0.17
Identities = 26/125 (20%), Positives = 60/125 (47%), Gaps = 1/125 (0%)
Frame = +3
Query: 22 LFLFC-NYVLLGAASSCIFLTLSLRLVPSVCGFFLILLHIFTIAGAVSGCAAVGANRWYS 80
L L C N+ + ++S T+ L++ P+ G L+++ ++ + G + + +
Sbjct: 48 LLLSCLNFSIFILSASSFAPTILLKMPPTSFGMALLMVSGISLLSSFVGFYSQLTHFCFL 227
Query: 81 AHMVATVLTAIFQGSVSVLVFTRTSDFLGELQSYVREEDGSVILKLSGGLAILIFCLEWV 140
H+ + + I Q + +FT+ + L+S ++ V+++L G + + L+ V
Sbjct: 228 THISLLLASLIGQVLTILALFTKEKASMSLLKSPRDPKEAKVLVRLECGALMAMCMLQCV 407
Query: 141 VLTLA 145
VL L+
Sbjct: 408 VLMLS 422
>CO985484
Length = 801
Score = 29.3 bits (64), Expect = 1.1
Identities = 15/52 (28%), Positives = 30/52 (56%), Gaps = 1/52 (1%)
Frame = +1
Query: 14 PRHYNIHKLFLFCNYVLLGAASSCIFLTLSLRLVP-SVCGFFLILLHIFTIA 64
P H N+H LF+F ++ ++ SC+ +T+S L + FF++ + T++
Sbjct: 550 PVHKNLHNLFMFDSHFVV----SCVLVTVSSNLSD*PIHSFFIVSCVLVTVS 693
>TC215931 similar to UP|O81618 (O81618) F8M12.11 protein, partial (25%)
Length = 1148
Score = 28.5 bits (62), Expect = 1.9
Identities = 18/57 (31%), Positives = 23/57 (39%)
Frame = -1
Query: 24 LFCNYVLLGAASSCIFLTLSLRLVPSVCGFFLILLHIFTIAGAVSGCAAVGANRWYS 80
LF N+ LL C L ++ I + IA V G AA+G RW S
Sbjct: 272 LFENF*LLFGTLLCFLFFGFLDILIMSSSVMSIFFSVSAIASVVRGLAAIGRRRWNS 102
>TC226446 homologue to UP|P2A_MEDSA (Q06009) Serine/threonine protein
phosphatase PP2A catalytic subunit , partial (80%)
Length = 1211
Score = 28.1 bits (61), Expect = 2.5
Identities = 9/24 (37%), Positives = 17/24 (70%)
Frame = +1
Query: 44 LRLVPSVCGFFLILLHIFTIAGAV 67
+ +VP+ CG+FL + H+F + +V
Sbjct: 1003 IHIVPAKCGYFLFVFHLFCLLCSV 1074
>TC206933 similar to UP|Q99322 (Q99322) Mucin (Fragment), partial (6%)
Length = 1812
Score = 27.7 bits (60), Expect = 3.2
Identities = 33/133 (24%), Positives = 55/133 (40%), Gaps = 5/133 (3%)
Frame = +3
Query: 23 FLFCNYVLLGAASSCIFLTLSLRLVPSVCGFFLILLHIFTIAGAVSGCAAVGANRWYSAH 82
F ++ +LG + LTL L S F I+ I + G V AV + +A
Sbjct: 1041 FSAVSFQVLGHMKTVCVLTLGWLLFDSELTFKNIMGMIIAVVGMVIYSWAVELEKQSNAK 1220
Query: 83 MVATVLTAIFQGSVSVL-VFTRTSDF----LGELQSYVREEDGSVILKLSGGLAILIFCL 137
+ V ++ + + +L V S LGE Q + D +++KL ++C+
Sbjct: 1221 TLPHVKNSMTEEEIRLLKVGVENSPLKDVELGEAQG*I---DSDLVVKLMRNECRQLYCV 1391
Query: 138 EWVVLTLAFFLKY 150
L AF+L Y
Sbjct: 1392 LLYNLVCAFYLVY 1430
>BI969502
Length = 643
Score = 26.9 bits (58), Expect = 5.5
Identities = 24/91 (26%), Positives = 40/91 (43%), Gaps = 11/91 (12%)
Frame = -3
Query: 69 GCAAVGANRWYSAHMVATVLT------AIFQGSVSVLVFTRTSDFLGELQSYVREEDGSV 122
GC A+ +R +++H++ + IF S V V+ R S F + V++ D
Sbjct: 290 GCTAIPTSRLHASHLIEQFKSL*ESSVGIFCFSFPVSVY*RPS*FASLHTNLVKQIDMRT 111
Query: 123 ILKLSGGLAI-LIFCLEW----VVLTLAFFL 148
+ L + C EW VV+T FF+
Sbjct: 110 CMNFYYLLKLKFCNCYEWHLFSVVITFVFFV 18
>TC215177 homologue to GB|AAP31934.1|30387537|BT006590 At2g35860 {Arabidopsis
thaliana;} , partial (10%)
Length = 768
Score = 26.9 bits (58), Expect = 5.5
Identities = 15/43 (34%), Positives = 23/43 (52%)
Frame = +1
Query: 19 IHKLFLFCNYVLLGAASSCIFLTLSLRLVPSVCGFFLILLHIF 61
I+K+ LFCNY+L ++SS P V + +I+ H F
Sbjct: 664 INKMILFCNYILYISSSS-----------PFVLPYNIIITHPF 759
>TC234348 UP|Q79UP5 (Q79UP5) Blr1854 protein, partial (19%)
Length = 425
Score = 26.6 bits (57), Expect = 7.2
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = -2
Query: 16 HYNIHKLFLFCNYVLLGAASSCIFLTLSLRLVPSVCGFFLI 56
H ++H + F L+ SC +SLRL + C FFL+
Sbjct: 412 HNSMHAAYWFAT--LVNRRLSCAGEAMSLRLSSTPCAFFLL 296
>TC215865 homologue to UP|ARGI_SOYBN (O49046) Arginase , complete
Length = 1335
Score = 26.6 bits (57), Expect = 7.2
Identities = 23/86 (26%), Positives = 33/86 (37%), Gaps = 2/86 (2%)
Frame = -1
Query: 84 VATVLTAIFQGSVSVLVFTRTSDFLGELQSY--VREEDGSVILKLSGGLAILIFCLEWVV 141
VAT + S LVF+RTS + + E G + S FC++W+
Sbjct: 276 VATAPPKLCTSSFLSLVFSRTSVSAASITRFWAFSSESGDIFSAHS-------FCMQWIP 118
Query: 142 LTLAFFLKYYACVEGGNTCRTVVLGS 167
L F + + E VLGS
Sbjct: 117 LRPTFDISLFLAKERRKLIVEQVLGS 40
>TC232981 UP|Q9LLR0 (Q9LLR0) Carboxyl transferase alpha subunit , complete
Length = 2799
Score = 26.6 bits (57), Expect = 7.2
Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Frame = -3
Query: 16 HYNIHKLFLFCNYVLLGAASSCIF-LTLSLRLVPSVCG 52
H ++ L LFC+ LGAA +C TLS ++PS G
Sbjct: 1966 HNSLR*LSLFCSLP*LGAARNCCSNSTLSFSILPSSNG 1853
>TC219056 similar to UP|Q9LVN1 (Q9LVN1) Gb|AAD23008.1, partial (7%)
Length = 833
Score = 26.6 bits (57), Expect = 7.2
Identities = 22/83 (26%), Positives = 39/83 (46%), Gaps = 4/83 (4%)
Frame = -3
Query: 13 TPRHYNIHKLFLFCNYVLLGAASSCIFLTLSLRL----VPSVCGFFLILLHIFTIAGAVS 68
TP + H++ + Y+L+ ASS I+L L +R+ + S FF +F I+ S
Sbjct: 450 TPDPFLSHQILVMLEYILILKASS-IYLALPVRIAVGKILSDSHFFFSFSAVFFISS--S 280
Query: 69 GCAAVGANRWYSAHMVATVLTAI 91
C W+S+ + +L +
Sbjct: 279 SCL------WFSSWALLNILVKL 229
>TC225464 GB|AAB68399.1|1773321|HAU79736 calmodulin {Helianthus annuus;} ,
partial (79%)
Length = 788
Score = 26.2 bits (56), Expect = 9.4
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = -2
Query: 117 EEDGSVILKLSGGLAILIFCLEWVV 141
E DGSV + ++ IL FCL WV+
Sbjct: 103 EIDGSVAISINLVYHILQFCLSWVL 29
>TC205826 similar to UP|KELP_ARATH (O65155) RNA polymerase II transcriptional
coactivator KELP, partial (46%)
Length = 865
Score = 26.2 bits (56), Expect = 9.4
Identities = 16/49 (32%), Positives = 27/49 (54%), Gaps = 3/49 (6%)
Frame = -1
Query: 136 CLEWVVLTLAF---FLKYYACVEGGNTCRTVVLGSAKVQQDEDLKDWPW 181
C+++ V TLAF FLK+ + G T +T + S+ + + +K PW
Sbjct: 793 CIQFEV-TLAFSLAFLKWLSSHSGCKTQKTFTVTSSPLTKRSSIKGAPW 650
>TC214336 homologue to UP|Q8L4F2 (Q8L4F2) 40S ribosomal protein S23, partial
(16%)
Length = 524
Score = 26.2 bits (56), Expect = 9.4
Identities = 21/65 (32%), Positives = 27/65 (41%)
Frame = +2
Query: 21 KLFLFCNYVLLGAASSCIFLTLSLRLVPSVCGFFLILLHIFTIAGAVSGCAAVGANRWYS 80
+L L LG C F L L + FF++LL FT A+ G A N +
Sbjct: 227 ELHLTFQVTYLGLPRLCFFPFLWLLAIV----FFILLL*TFTEYIAIEGLAIASVNESIN 394
Query: 81 AHMVA 85
MVA
Sbjct: 395 REMVA 409
>TC206981 similar to UP|Q9LQB4 (Q9LQB4) F4N2.3, partial (25%)
Length = 1981
Score = 26.2 bits (56), Expect = 9.4
Identities = 13/50 (26%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Frame = -2
Query: 55 LILLHIFTIAGA-VSGCAAVGANRWYSAHMVATVLTAIFQGSVSVLVFTR 103
LILL+IFTI G + G +++ + ++ ++ ++ +L+ TR
Sbjct: 813 LILLNIFTIGGTRICGASSILFGHIFLVNIKVYIIITMYSRRTGMLIITR 664
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.328 0.142 0.449
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,315,655
Number of Sequences: 63676
Number of extensions: 159643
Number of successful extensions: 1317
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 1309
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1315
length of query: 185
length of database: 12,639,632
effective HSP length: 91
effective length of query: 94
effective length of database: 6,845,116
effective search space: 643440904
effective search space used: 643440904
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC147179.2