BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146308.4 + phase: 0
(357 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC230630 similar to UP|Q9LR86 (Q9LR86) T23E23.24, partial (27%) 226 9e-60
TC220664 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial... 166 2e-41
TC230629 similar to UP|Q84TE0 (Q84TE0) At5g51080, partial (13%) 125 4e-29
TC232459 weakly similar to UP|Q9LR86 (Q9LR86) T23E23.24, partial... 124 5e-29
TC233372 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial... 119 2e-27
TC208902 53 2e-07
BI498328 36 0.023
TC219971 UP|Q6T2Z4 (Q6T2Z4) Wee1, complete 31 0.75
BF071657 30 1.3
TC227335 similar to UP|Q9LP69 (Q9LP69) T1N15.17, partial (16%) 30 1.7
CD416211 30 1.7
TC232019 homologue to UP|Q9LEN2 (Q9LEN2) NAD-dependent malic enz... 30 2.2
TC216131 similar to UP|Q9M3V1 (Q9M3V1) Protein phpsphatase 2C (P... 30 2.2
TC212032 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, part... 29 2.9
TC207442 similar to UP|Q9FKZ9 (Q9FKZ9) Gb|AAB71479.1|, partial (... 28 4.9
TC225167 similar to GB|CAA44226.1|20020|NTRPL121A ribosomal prot... 28 4.9
TC230546 similar to GB|AAM62506.1|21553413|AY085274 VAMP (vesicl... 28 6.4
AW832520 28 6.4
>TC230630 similar to UP|Q9LR86 (Q9LR86) T23E23.24, partial (27%)
Length = 825
Score = 226 bits (577), Expect = 9e-60
Identities = 130/233 (55%), Positives = 156/233 (66%), Gaps = 26/233 (11%)
Frame = +1
Query: 6 SHLSSFTSTILARTTQFVANRSLHTTPHTFSFPVKSHHSLLTSFHSQFVLTTVTRCYSTR 65
S LSS+T++I+ T + +ANRSLH S P K + + SF S+F +TT RCYS +
Sbjct: 172 SQLSSYTASIVGSTVRLIANRSLHH--QCCSLPAKPEYRGIRSFRSEFAVTT--RCYSAK 339
Query: 66 KLRKTGSSKLHKVEAETTMNQEQNESDAFYVVRKGDVVGIYNTLTDSQAQVGSSVCDPPV 125
K RK+ KVE E + E DAFYVVRKGDVVGIYN+L DSQAQVGSSVC+PPV
Sbjct: 340 KGRKS------KVEPEAM----KQEKDAFYVVRKGDVVGIYNSLADSQAQVGSSVCNPPV 489
Query: 126 SVYKGYSMSNETEEYLLSHGLKNALYTIRASDLKEDLFGTLVPCPFQDPSSTQGTTSNAD 185
SVYKGYS+S +TEEYL+SHGLKNALYTIRA+DLKEDLFG LVPCPFQ+PS+ +G TSN D
Sbjct: 490 SVYKGYSLSKDTEEYLVSHGLKNALYTIRATDLKEDLFGMLVPCPFQEPSTKEG-TSNKD 666
Query: 186 SSKKRALEVLEQNN--------------------------VKTCIVEFDGASK 212
SK+R+L VL Q+ +TC VEFDGASK
Sbjct: 667 VSKQRSLGVLAQDEKVISEDPFRKQVKLEYAEVAEAPSHATRTCFVEFDGASK 825
>TC220664 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial (31%)
Length = 724
Score = 166 bits (420), Expect = 2e-41
Identities = 86/104 (82%), Positives = 93/104 (88%)
Frame = +1
Query: 232 IYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSICIQGDSKLVCMQMKQIDGLWKVKN 291
I R+REGVGIATNN AEYRAMILGM++ALKKGFT I IQGDSKLVCMQ IDG WKVKN
Sbjct: 31 ICRLREGVGIATNNAAEYRAMILGMKYALKKGFTGIRIQGDSKLVCMQ---IDGSWKVKN 201
Query: 292 ENLSTLYKVAKELKDKFVSFKISHVLRDLNSEADAQANLAVNLA 335
ENLSTLY VAKELKDKF SF+ISHVLR+ NS+ADAQANLA+NLA
Sbjct: 202 ENLSTLYNVAKELKDKFSSFQISHVLRNFNSDADAQANLAINLA 333
>TC230629 similar to UP|Q84TE0 (Q84TE0) At5g51080, partial (13%)
Length = 560
Score = 125 bits (313), Expect = 4e-29
Identities = 73/131 (55%), Positives = 86/131 (64%)
Frame = +3
Query: 1 MNALFSHLSSFTSTILARTTQFVANRSLHTTPHTFSFPVKSHHSLLTSFHSQFVLTTVTR 60
MN L S LSS+T+ I+ T + +ANRSL SFP K + + SF S+F LT R
Sbjct: 198 MNRL-SQLSSYTAAIVGSTVRLIANRSLRH--RCCSFPAKPEYRGIRSFRSEFALTAA-R 365
Query: 61 CYSTRKLRKTGSSKLHKVEAETTMNQEQNESDAFYVVRKGDVVGIYNTLTDSQAQVGSSV 120
CYS +K RK+ K E E + E DAFYVVRKGDVVGIYN+L DSQAQVGSSV
Sbjct: 366 CYSAKKGRKS------KAEPEVPAVVMEQEKDAFYVVRKGDVVGIYNSLADSQAQVGSSV 527
Query: 121 CDPPVSVYKGY 131
C+PPVSVYKGY
Sbjct: 528 CNPPVSVYKGY 560
>TC232459 weakly similar to UP|Q9LR86 (Q9LR86) T23E23.24, partial (19%)
Length = 483
Score = 124 bits (312), Expect = 5e-29
Identities = 60/100 (60%), Positives = 73/100 (73%)
Frame = +3
Query: 90 ESDAFYVVRKGDVVGIYNTLTDSQAQVGSSVCDPPVSVYKGYSMSNETEEYLLSHGLKNA 149
E DAFYVVRKGDVVGIY + +D Q + SSV PVS+YKGYS+ +TEEYL+SHGLK A
Sbjct: 153 EKDAFYVVRKGDVVGIYKSFSDIQPLLASSVSSDPVSIYKGYSLPQKTEEYLVSHGLKGA 332
Query: 150 LYTIRASDLKEDLFGTLVPCPFQDPSSTQGTTSNADSSKK 189
Y+I A+D+ E LFG LV CP+QDP S+ G N SS +
Sbjct: 333 PYSISAADVNEGLFGRLVACPYQDPYSSGGRAFNVSSSSR 452
>TC233372 weakly similar to UP|Q84TE0 (Q84TE0) At5g51080, partial (12%)
Length = 561
Score = 119 bits (298), Expect = 2e-27
Identities = 58/88 (65%), Positives = 70/88 (78%)
Frame = +1
Query: 247 AEYRAMILGMRHALKKGFTSICIQGDSKLVCMQMKQIDGLWKVKNENLSTLYKVAKELKD 306
AEYR++ILG+ H KKG+ I +QGDS LVC QI GLWK+KN+N+ TL AKELKD
Sbjct: 1 AEYRSLILGLXHXXKKGYKXIIVQGDSLLVC---NQIQGLWKIKNQNMGTLCXEAKELKD 171
Query: 307 KFVSFKISHVLRDLNSEADAQANLAVNL 334
KF+SFKISH+ R+ NSEADAQANLA+NL
Sbjct: 172 KFLSFKISHIPREYNSEADAQANLAINL 255
>TC208902
Length = 838
Score = 53.1 bits (126), Expect = 2e-07
Identities = 37/129 (28%), Positives = 68/129 (52%), Gaps = 2/129 (1%)
Frame = +1
Query: 208 DGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSI 267
DGA G G A G +LR++ G ++ +G+ + + AE A+ +G++ A KGFT++
Sbjct: 247 DGAVSG--GVAACGRVLRNQAGAVVV-FAHNLGMCSISQAEIWAITMGVKLAWDKGFTNL 417
Query: 268 CIQGDSKLVCMQMKQIDGLWKVKNENLSTLYKVAKELKDKF--VSFKISHVLRDLNSEAD 325
++ D K ++ +DG+ + N +++ K KD ++ H+LR+ N AD
Sbjct: 418 YVESDFKYA---IEMMDGVCEFSNN----CFQLVKSAKDSV*GETYSWCHILREANKTAD 576
Query: 326 AQANLAVNL 334
A A V++
Sbjct: 577 ALAKFGVDV 603
>BI498328
Length = 335
Score = 36.2 bits (82), Expect = 0.023
Identities = 27/63 (42%), Positives = 35/63 (54%), Gaps = 1/63 (1%)
Frame = +1
Query: 199 NVKTCIVEFDGASKGNPGKAGAGAILRSKDGNLI-YRVREGVGIATNNVAEYRAMILGMR 257
++ IV FDGAS N G GA+L S D I + R G TNN+AEY A LG++
Sbjct: 139 DINKWIVCFDGAS--NALGHGVGAVLVSPDDQCIPFTARLGFD-CTNNMAEYEACALGVQ 309
Query: 258 HAL 260
A+
Sbjct: 310 AAI 318
>TC219971 UP|Q6T2Z4 (Q6T2Z4) Wee1, complete
Length = 1860
Score = 31.2 bits (69), Expect = 0.75
Identities = 27/92 (29%), Positives = 38/92 (40%), Gaps = 1/92 (1%)
Frame = +1
Query: 121 CDPPVSVYKGYSMSNETEEYLLSHGLKNALYTIRASDLKEDL-FGTLVPCPFQDPSSTQG 179
CD +S+ K S S +K AL T + L G + P Q PSST
Sbjct: 25 CDSSLSMVKMKGKSQSQRIGKKSRKMKGALNTHTNTTRHLQLQLGQVSLIPLQQPSSTTA 204
Query: 180 TTSNADSSKKRALEVLEQNNVKTCIVEFDGAS 211
TTS+A S + L+ N + + D A+
Sbjct: 205 TTSSAVSCFQNLLDAEAPNASSSRVALIDNAA 300
>BF071657
Length = 415
Score = 30.4 bits (67), Expect = 1.3
Identities = 13/50 (26%), Positives = 28/50 (56%)
Frame = -1
Query: 295 STLYKVAKELKDKFVSFKISHVLRDLNSEADAQANLAVNLAGEAFKIINV 344
STL + + + +++ H+++++N+ ADA AN ++L +I V
Sbjct: 415 STLVRYINSMLEPTITYFCRHIVQEINAPADALANYGMSLDNGNLRIFQV 266
>TC227335 similar to UP|Q9LP69 (Q9LP69) T1N15.17, partial (16%)
Length = 713
Score = 30.0 bits (66), Expect = 1.7
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = -1
Query: 113 QAQVGSSVCDPPVSVYKGYSMSNETEEYLLSHGLKNALYTIRA 155
Q + SSV DPPV ++K S+ ++E L L+N I A
Sbjct: 536 QINIMSSVLDPPVMIFKITSIFYQSESLSLDERLRNVCQHIAA 408
>CD416211
Length = 551
Score = 30.0 bits (66), Expect = 1.7
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = -3
Query: 202 TCIVEFDGASKGNPGKAGAGAILRSKDGNL 231
T + DG+S GNPG +G G + R++ G++
Sbjct: 459 TIKINTDGSSFGNPGISGYGGVFRNEHGHV 370
>TC232019 homologue to UP|Q9LEN2 (Q9LEN2) NAD-dependent malic enzyme (Malate
oxidoreductase) (Fragment) , partial (37%)
Length = 616
Score = 29.6 bits (65), Expect = 2.2
Identities = 19/58 (32%), Positives = 24/58 (40%)
Frame = -2
Query: 130 GYSMSNETEEYLLSHGLKNALYTIRASDLKEDLFGTLVPCPFQDPSSTQGTTSNADSS 187
GY S EY H L T + D+ E G PCP SST ++ A +S
Sbjct: 345 GYYFS*TRGEYTGYHILLRTYSTASSFDIWESSLGPTSPCPSASSSSTAARSTAAPTS 172
>TC216131 similar to UP|Q9M3V1 (Q9M3V1) Protein phpsphatase 2C (PP2C) ,
partial (47%)
Length = 1320
Score = 29.6 bits (65), Expect = 2.2
Identities = 17/39 (43%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = -3
Query: 22 FVANRSLHTTPHTFSFPVKSHHSLLTSF-HSQFVLTTVT 59
F A R + TTPH SF SH+ L S HS + +VT
Sbjct: 751 FCALRHILTTPHAVSFDTTSHNPSLASIKHSSSSVRSVT 635
>TC212032 similar to UP|Q6WAY3 (Q6WAY3) Gag/pol polyprotein, partial (3%)
Length = 803
Score = 29.3 bits (64), Expect = 2.9
Identities = 21/62 (33%), Positives = 28/62 (44%)
Frame = +2
Query: 269 IQGDSKLVCMQMKQIDGLWKVKNENLSTLYKVAKELKDKFVSFKISHVLRDLNSEADAQA 328
+ GDS LV Q++ G + ++ NL KEL F HV + N ADA A
Sbjct: 245 VYGDSALVIHQLR---GECETRDPNLIPYQAYIKELAGFFDEISFHHVA*EENQMADALA 415
Query: 329 NL 330
L
Sbjct: 416 TL 421
>TC207442 similar to UP|Q9FKZ9 (Q9FKZ9) Gb|AAB71479.1|, partial (26%)
Length = 1378
Score = 28.5 bits (62), Expect = 4.9
Identities = 21/89 (23%), Positives = 40/89 (44%), Gaps = 10/89 (11%)
Frame = +3
Query: 223 ILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFTSICIQ----------GD 272
IL+ K G+ V + NNV + ++LGM+ KG + ++
Sbjct: 807 ILKEKQGSEKLSVHD-----INNVEDRYEILLGMKELASKGMAAEAVEEVNAIDSNFFAQ 971
Query: 273 SKLVCMQMKQIDGLWKVKNENLSTLYKVA 301
+ ++ Q+KQ++ L V + + + KVA
Sbjct: 972 NSILLFQLKQVEFLKLVSSGDYNAALKVA 1058
>TC225167 similar to GB|CAA44226.1|20020|NTRPL121A ribosomal protein L12-1a
{Nicotiana tabacum;} , partial (70%)
Length = 993
Score = 28.5 bits (62), Expect = 4.9
Identities = 18/57 (31%), Positives = 24/57 (41%)
Frame = -3
Query: 209 GASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAEYRAMILGMRHALKKGFT 265
GA++G G + G + RVR G G VAE H +KKGF+
Sbjct: 172 GAARGGGAVGEVGELEGGGVGGEVRRVRRGGGCRIREVAEGEGC*CS*GHFVKKGFS 2
>TC230546 similar to GB|AAM62506.1|21553413|AY085274 VAMP (vesicle-associated
membrane protein)-associated protein-like {Arabidopsis
thaliana;} , partial (60%)
Length = 1019
Score = 28.1 bits (61), Expect = 6.4
Identities = 24/106 (22%), Positives = 46/106 (42%), Gaps = 2/106 (1%)
Frame = -2
Query: 12 TSTILARTTQFVANRSLHTTPHTFSFPVKSHHSLLTSFHSQFVLTTVTRCYSTRKLRKTG 71
T+ +L RT F L FS L + SQ+V C+S +++
Sbjct: 388 TTPVLGRTKYFFGEVVLTLKAT*FSV-------LFRTLRSQYV------CFSNSNWKRSS 248
Query: 72 S--SKLHKVEAETTMNQEQNESDAFYVVRKGDVVGIYNTLTDSQAQ 115
S + +++ EAE M +++ +FY + ++ T+T +A+
Sbjct: 247 SGLTVINEFEAEAVMTMKRSIDQSFYKNHQSSRTSMHTTITT*EAK 110
>AW832520
Length = 423
Score = 28.1 bits (61), Expect = 6.4
Identities = 14/44 (31%), Positives = 20/44 (44%)
Frame = -3
Query: 205 VEFDGASKGNPGKAGAGAILRSKDGNLIYRVREGVGIATNNVAE 248
+ +DGA N K AG ++R GN + T N+AE
Sbjct: 220 LNYDGAVNCNSLKVSAGGVVRDSHGNFVIGFTTTPDSCTINIAE 89
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.131 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,285,507
Number of Sequences: 63676
Number of extensions: 172311
Number of successful extensions: 1137
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1132
length of query: 357
length of database: 12,639,632
effective HSP length: 98
effective length of query: 259
effective length of database: 6,399,384
effective search space: 1657440456
effective search space used: 1657440456
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146308.4