BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144514.8 - phase: 0 /pseudo
(555 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC211561 weakly similar to UP|O22675 (O22675) Reverse transcript... 83 3e-16
CD417230 weakly similar to GP|2244915|emb| reverse transcriptase... 79 5e-15
CO982349 78 9e-15
AW757033 64 2e-10
TC231922 58 1e-08
TC213061 55 6e-08
BG404993 55 8e-08
CA851073 55 8e-08
CO986021 54 2e-07
TC233956 52 5e-07
TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR ret... 39 6e-07
BE610670 51 2e-06
BI471325 50 3e-06
TC221865 50 3e-06
BI973267 49 8e-06
BM527030 48 1e-05
CF921567 47 2e-05
BI974689 47 2e-05
CO979823 46 4e-05
TC231841 46 5e-05
>TC211561 weakly similar to UP|O22675 (O22675) Reverse transcriptase
(Fragment) , partial (59%)
Length = 1077
Score = 83.2 bits (204), Expect = 3e-16
Identities = 44/124 (35%), Positives = 69/124 (55%)
Frame = +1
Query: 52 VMVKTGFNNRWIY*MSMCVETVDFSVLVNNDAVGPFIPGRGLRQGDPLSPYMFILCVEGL 111
+M + F+ +WI + CV++ SVLVN F+P RGLRQGDPL+P++F + EGL
Sbjct: 127 MMWRMDFSPKWIKWIEECVKSASISVLVNGSPTAEFLPQRGLRQGDPLTPFLFNIVAEGL 306
Query: 112 SSLIRDAEEHRIISGISICRGAPSISHLLFADDCFLFFKADEGQTQVMKNILITYKAASG 171
+ L+R A E + + IS L +ADD F +A + + +K IL +++ S
Sbjct: 307 NGLMRRAVEENLYKPYLVGANGVPISILQYADDTIFFGEAAKENVEAIKVILRSFELVSN 486
Query: 172 QAIS 175
I+
Sbjct: 487 LRIN 498
>CD417230 weakly similar to GP|2244915|emb| reverse transcriptase like
protein {Arabidopsis thaliana}, partial (7%)
Length = 688
Score = 79.0 bits (193), Expect = 5e-15
Identities = 40/108 (37%), Positives = 61/108 (56%)
Frame = -3
Query: 69 CVETVDFSVLVNNDAVGPFIPGRGLRQGDPLSPYMFILCVEGLSSLIRDAEEHRIISGIS 128
C+ T S+L N + + F P G+RQ DP++PY+F+LC+E LS LI ++ I
Sbjct: 668 CMSTTTMSLLWNGEVLDHFSPNIGVRQRDPIAPYLFVLCLERLSHLINLVMNKKL*RSIQ 489
Query: 129 ICRGAPSISHLLFADDCFLFFKADEGQTQVMKNILITYKAASGQAISL 176
+ RG ISHL F DD LF +A+ Q V+ L + +SG +++
Sbjct: 488 VNRGGLLISHLAFVDDLILFVEANMDQVDVINLTLELFSDSSGAKVNV 345
>CO982349
Length = 795
Score = 78.2 bits (191), Expect = 9e-15
Identities = 39/107 (36%), Positives = 62/107 (57%)
Frame = +3
Query: 69 CVETVDFSVLVNNDAVGPFIPGRGLRQGDPLSPYMFILCVEGLSSLIRDAEEHRIISGIS 128
C+ + S+LVN + F P RGLRQGDPL+P +F + EGL+ L+R+A + + +
Sbjct: 81 CLHSASVSILVNESPINEFSPQRGLRQGDPLAPLLFNIVAEGLTGLMREAVDRKRFNSFL 260
Query: 129 ICRGAPSISHLLFADDCFLFFKADEGQTQVMKNILITYKAASGQAIS 175
+ + +S L +ADD F +A +V+K IL ++ ASG I+
Sbjct: 261 VGKNKEPVSILQYADDTIFFEEATMENMKVIKTILRCFELASGLKIN 401
>AW757033
Length = 441
Score = 63.5 bits (153), Expect = 2e-10
Identities = 34/89 (38%), Positives = 51/89 (57%)
Frame = -2
Query: 87 FIPGRGLRQGDPLSPYMFILCVEGLSSLIRDAEEHRIISGISICRGAPSISHLLFADDCF 146
F P RGLRQGDPL+P +F + EGL+ L+R+A ++ + +S L +ADD
Sbjct: 431 FSPHRGLRQGDPLAPLLFNIAAEGLTGLMREAVARNHYKSFAVGKYKKPVSILQYADDTI 252
Query: 147 LFFKADEGQTQVMKNILITYKAASGQAIS 175
F +A +V+K IL ++ ASG I+
Sbjct: 251 FFGEATMENVRVIKTILRGFELASGLKIN 165
>TC231922
Length = 803
Score = 57.8 bits (138), Expect = 1e-08
Identities = 36/122 (29%), Positives = 57/122 (46%), Gaps = 1/122 (0%)
Frame = -3
Query: 431 DIAYDVLNTSLVNQVTNDKLIWKAEKNGIYSVKSTFRLCVEDLMDTTHLRCPEFWMGIWR 490
D ++ S+ NQ +D + WKA+ +G+YS KS +RL + + R + +W
Sbjct: 492 DFMNEISGISIQNQA-HDSMFWKADSSGVYSTKSAYRLLMPSISPAPSRR---IFQILWH 325
Query: 491 LKVPPKIKKSHVDDMLDEILPTRMQLKDKGV*C-PTYCVSYEHQEEDLEHIFFQCPFSVQ 549
LK+PP+ LD LPTR L + + C Q E+ H+FF C +
Sbjct: 324 LKIPPRAAVFSWRLFLDR-LPTRGNLSRRSIPIQDIMCPLCGCQHEEAGHLFFHCKMTKG 148
Query: 550 VW 551
+W
Sbjct: 147 LW 142
>TC213061
Length = 823
Score = 55.5 bits (132), Expect = 6e-08
Identities = 42/132 (31%), Positives = 61/132 (45%), Gaps = 1/132 (0%)
Frame = +2
Query: 421 VPFIQ*VFSKDIAYDVLNTSLVNQVTNDKLIWKAEKNGIYSVKSTFRLCVEDLMDTTHLR 480
V FIQ V I D+ D+ +W AE +G Y +S +R+ E + + R
Sbjct: 77 VAFIQEVEGHKIRSDI----------EDQWVWAAESSGSYLARSAYRVIREGIPEEEQDR 226
Query: 481 CPEFWMGIWRLKVPPKIKKSHVDDMLDEILPTRMQLKDKGV*CPTY-CVSYEHQEEDLEH 539
EF +W+LKVP K+ +L +ILPTR L+ K V Y C +E H
Sbjct: 227 --EF-KELWKLKVPMKVTM-FAWRLLKDILPTRDNLRRKRVELHEYVCPLCRSMDESASH 394
Query: 540 IFFQCPFSVQVW 551
+FF C + +W
Sbjct: 395 LFFHCSKILPIW 430
>BG404993
Length = 412
Score = 55.1 bits (131), Expect = 8e-08
Identities = 32/116 (27%), Positives = 57/116 (48%), Gaps = 1/116 (0%)
Frame = -3
Query: 437 LNTSLVNQVTNDKLIWKAEKNGIYSVKSTFRLCVEDLMDTTHLRCPEFWMGIWRLKVPPK 496
+ S + Q D WKA+ +G YS K+ + L E + ++ +W+LK+PPK
Sbjct: 365 IGLSTIQQHEADSWTWKADPSGNYSTKTAYELLQEAIRGDNE---DGIFVQLWKLKIPPK 195
Query: 497 IKKSHVDDMLDEILPTRMQLKDKGV*CP-TYCVSYEHQEEDLEHIFFQCPFSVQVW 551
++++ LPT++ L+ + V + C + EED H+FF C ++ W
Sbjct: 194 APV-FTWRLINDRLPTKVNLRRRQVEISDSLCPLCNNSEEDAAHLFFNCNKTLPFW 30
>CA851073
Length = 633
Score = 55.1 bits (131), Expect = 8e-08
Identities = 30/105 (28%), Positives = 54/105 (50%), Gaps = 1/105 (0%)
Frame = +1
Query: 448 DKLIWKAEKNGIYSVKSTFRLCVEDLMDTTHLRCPEFWMGIWRLKVPPKIKKSHVDDMLD 507
D ++WKA+ G+YS KS +R+ + H+ + IW+LK+PP+ ++
Sbjct: 28 DTMVWKADPCGVYSTKSAYRIL---MTCNRHVSEANIFKTIWKLKIPPRAAVFSW-RLIK 195
Query: 508 EILPTRMQLKDKGV*C-PTYCVSYEHQEEDLEHIFFQCPFSVQVW 551
+ LPTR L + V C +++E+ +H+FF C + +W
Sbjct: 196 DRLPTRHNLLRRNVSIQENECPLCGYEQEEADHLFFNCKMTRGLW 330
>CO986021
Length = 900
Score = 53.5 bits (127), Expect = 2e-07
Identities = 38/142 (26%), Positives = 69/142 (47%), Gaps = 9/142 (6%)
Frame = -1
Query: 419 WDVPFIQ*VFSKD----IAY-DVLNTSLVNQVTNDKLIWKAEKNGIYSVKSTFRLCVEDL 473
WD + + +F + IA+ D ++ ++Q D ++WKA+ GIYS KS +RL + +
Sbjct: 885 WDFKWRRNLFDHENEQAIAFMDDISAISIHQQLQDSMLWKADPTGIYSTKSAYRLLLPNN 706
Query: 474 MDTTHLRCPEFWMGIWRLKVPPKIK----KSHVDDMLDEILPTRMQLKDKGV*CPTYCVS 529
H + +W+LK+PP+ + + D + R + + + CP +
Sbjct: 705 RPGQH---SSNFKILWKLKIPPRAELFSWRLFRDRLSIRANLLRRHVALQDIMCP---LC 544
Query: 530 YEHQEEDLEHIFFQCPFSVQVW 551
HQEE H+FF C ++ +W
Sbjct: 543 GNHQEE-AGHLFFHCRMTIGLW 481
>TC233956
Length = 757
Score = 52.4 bits (124), Expect = 5e-07
Identities = 40/123 (32%), Positives = 62/123 (49%), Gaps = 6/123 (4%)
Frame = +2
Query: 435 DVLNTSLVNQVTNDKLIWKAEKNGIYSVKSTFRLCVEDLMDT-THLRCPEFWMGIWRLKV 493
D ++ +N ND +W+AE GI S KS +++ +L D HL + +W +KV
Sbjct: 20 DHIHEIRLNNNFNDTWVWRAESTGIISTKSAYQVIKSELDDEGQHLGFKK----LWEIKV 187
Query: 494 PPKIKKSHVDDMLDEILPTR-----MQLKDKGV*CPTYCVSYEHQEEDLEHIFFQCPFSV 548
PPK S V +L + LPT+ Q++ + CP +C S Q E H+FF C +
Sbjct: 188 PPKAL-SFVWRLLWDRLPTKDNLIKRQIQVEDDLCP-FCHS---QSETASHLFFTCGKIM 352
Query: 549 QVW 551
+W
Sbjct: 353 PLW 361
>TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR retroelement
reverse transcriptase, partial (7%)
Length = 956
Score = 38.9 bits (89), Expect(2) = 6e-07
Identities = 19/54 (35%), Positives = 34/54 (62%)
Frame = -3
Query: 51 AVMVKTGFNNRWIY*MSMCVETVDFSVLVNNDAVGPFIPGRGLRQGDPLSPYMF 104
+V+ K G+++ + + + + + S+ VN + VG F RG+RQGDPLSP ++
Sbjct: 705 SVLNKFGYSHTFCNWIRVILLSAKLSISVNGEPVGFFSCQRGVRQGDPLSPSLY 544
Score = 32.7 bits (73), Expect(2) = 6e-07
Identities = 18/55 (32%), Positives = 28/55 (50%)
Frame = -2
Query: 128 SICRGAPSISHLLFADDCFLFFKADEGQTQVMKNILITYKAASGQAISLPISEIY 182
S+ G+ + SH++ ADD +F K + N YK + GQ IS S+I+
Sbjct: 478 SVPMGSLTRSHVMHADDIMIFCKGTSRNVHNLINFFQLYKDSFGQVISKHKSKIF 314
>BE610670
Length = 368
Score = 50.8 bits (120), Expect = 2e-06
Identities = 32/105 (30%), Positives = 52/105 (49%), Gaps = 1/105 (0%)
Frame = -2
Query: 448 DKLIWKAEKNGIYSVKSTFRLCVEDLMDTTHLRCPEFWMGIWRLKVPPKIKKSHVDDMLD 507
D LIW+A+ +G YS KS + L + + IW LK+PP+ + +
Sbjct: 313 DSLIWRADPSGAYSTKSAYNLLKGEGSFDNEDSASKI---IWNLKIPPR-TIAFSWRIFK 146
Query: 508 EILPTRMQLKDKGV*CPTY-CVSYEHQEEDLEHIFFQCPFSVQVW 551
LPT+ L+ + V P+Y C + +EE++ HI F C + +W
Sbjct: 145 NRLPTKANLRRRQVELPSYRCPLCDLEEENVGHIMFSCTRTRSLW 11
>BI471325
Length = 421
Score = 50.1 bits (118), Expect = 3e-06
Identities = 28/78 (35%), Positives = 45/78 (56%)
Frame = +2
Query: 124 ISGISICRGAPSISHLLFADDCFLFFKADEGQTQVMKNILITYKAASGQAISLPISEIYC 183
I + + RG +SHLL D FLF + + + V+KNIL T++A+SG ++L SEI
Sbjct: 8 IHRVKVHRGITILSHLLSVDFYFLFCRTIDKEADVLKNILTTFEASSGLVVNLRKSEICF 187
Query: 184 NQNVFDALKASITNIIGV 201
++N + SI +G+
Sbjct: 188 SRNTSQIARDSIFFHLGM 241
>TC221865
Length = 1045
Score = 50.1 bits (118), Expect = 3e-06
Identities = 33/111 (29%), Positives = 54/111 (47%), Gaps = 1/111 (0%)
Frame = -3
Query: 442 VNQVTNDKLIWKAEKNGIYSVKSTFRLCVEDLMDTTHLRCPEFWMGIWRLKVPPKIKKSH 501
+++ D +WK+E NG YS +S + L + P F IW+LK+P K
Sbjct: 686 IHRHLTDCWVWKSEPNGHYSTRSAYHLMQHEAAKAN--TDPAF-EEIWKLKIPAKAA-IF 519
Query: 502 VDDMLDEILPTRMQLKDKGV*CP-TYCVSYEHQEEDLEHIFFQCPFSVQVW 551
++ + LPT+ L+ + V T C+ + EE+ H+FF C + VW
Sbjct: 518 A*RLVKDRLPTKNNLRRRQVQLNGTLCLFCRNYEEEASHLFFSCTKTQPVW 366
>BI973267
Length = 406
Score = 48.5 bits (114), Expect = 8e-06
Identities = 33/118 (27%), Positives = 57/118 (47%), Gaps = 1/118 (0%)
Frame = +1
Query: 435 DVLNTSLVNQVTNDKLIWKAEKNGIYSVKSTFRLCVEDLMDTTHLRCPEFWMGIWRLKVP 494
D ++ S +N D W+AE NG ++ KST+++ +L D E + + +K+P
Sbjct: 22 DQIHASSLNINLKDTWTWRAEANGEFTTKSTYQVLQSELDDQGQY---EGFQQLCNIKIP 192
Query: 495 PKIKKSHVDDMLDEILPTRMQLKDKGV*CPT-YCVSYEHQEEDLEHIFFQCPFSVQVW 551
PK S +L + LPT+ L + + C +Q E H+FF+C + +W
Sbjct: 193 PK-ALSFAWRLL*DRLPTKDNLVKRQIQVENDLCPFCHNQPETASHLFFKCDKIMPMW 363
>BM527030
Length = 421
Score = 48.1 bits (113), Expect = 1e-05
Identities = 33/113 (29%), Positives = 47/113 (41%), Gaps = 8/113 (7%)
Frame = +3
Query: 448 DKLIWKAEKNGIYSVKSTFRLCVEDLMDTTHLRCPEFWMGIWRLKVPPKIKKSHVDDMLD 507
D L+WK Y V+ + +E+ L F+ IW L +P + D
Sbjct: 90 DSLVWKLALTECYFVRDPYLFLIENTNPVYVL----FYRSIWMLAIPHNVAIFEWKLSKD 257
Query: 508 EILPTRMQLKDKGV*CPTYCVSYEHQ--------EEDLEHIFFQCPFSVQVWQ 552
+ LPTR L+ C + +HQ EED H+ F C FS Q+WQ
Sbjct: 258 K-LPTRKNLQ-----CRNILLEEQHQLCPFCSGKEEDSSHLIFTCSFSYQIWQ 398
>CF921567
Length = 686
Score = 47.0 bits (110), Expect = 2e-05
Identities = 30/99 (30%), Positives = 48/99 (48%), Gaps = 1/99 (1%)
Frame = -1
Query: 454 AEKNGIYSVKSTFRLCVEDLMDTTHLRCPEFWMGIWRLKVPPKIKKSHVDDMLDEILPTR 513
++ NG+YS KS +++ E R +W LK+PP+ + + LPTR
Sbjct: 686 SDPNGLYSTKSAYKVL*EGHASAIEDRVLNI---MWSLKIPPRAS-AFSWRLFKNRLPTR 519
Query: 514 MQLKDKGV*CPTY-CVSYEHQEEDLEHIFFQCPFSVQVW 551
LK + V TY C + +EE + H+FF C + +W
Sbjct: 518 ENLKRRQVSLHTYSCPLCDLEEESVNHLFFNCSKTRSLW 402
>BI974689
Length = 409
Score = 47.0 bits (110), Expect = 2e-05
Identities = 25/61 (40%), Positives = 37/61 (59%), Gaps = 1/61 (1%)
Frame = -1
Query: 485 WMGIWRLKVPPKIKKSHVDDMLDEILPTRMQLKDKGV*CPTYCVSYEHQEEDLE-HIFFQ 543
W IW LKVPPK+K + + + LP+R++L KGV CP+ C + E L+ ++ FQ
Sbjct: 388 WKKIWALKVPPKVKHL-IRRIQRDCLPSRVRLHAKGVQCPSTCAFCHNNLESLDVNM*FQ 212
Query: 544 C 544
C
Sbjct: 211 C 209
>CO979823
Length = 853
Score = 46.2 bits (108), Expect = 4e-05
Identities = 32/105 (30%), Positives = 52/105 (49%), Gaps = 1/105 (0%)
Frame = -2
Query: 448 DKLIWKAEKNGIYSVKSTFRLCVEDLMDTTHLRCPEFWMGIWRLKVPPKIKKSHVDDMLD 507
DK +WK E G YS KS + + +L T ++ +F IW+LK+P K ++
Sbjct: 702 DKWLWKPEPGGHYSTKSGYHVLWGEL--TEEIQDADF-AEIWKLKIPTKAAV-FAWRLVR 535
Query: 508 EILPTRMQLKDKGV*CPTY-CVSYEHQEEDLEHIFFQCPFSVQVW 551
+ LPT+ L+ + V C + EE H+FF C ++ +W
Sbjct: 534 DRLPTKSNLRRRQVMVQDMVCPLCNNIEEGAAHLFFNCTKTLPLW 400
>TC231841
Length = 791
Score = 45.8 bits (107), Expect = 5e-05
Identities = 32/109 (29%), Positives = 53/109 (48%), Gaps = 5/109 (4%)
Frame = +3
Query: 448 DKLIWKAEKNGIYSVKSTFRLCVEDLMDTTHLRCPEFWMGIWRLKVPPKIKKSHVDDMLD 507
D+ +WKA+ +G Y+ KS + + ++ + + +W+LK+P KI ++
Sbjct: 63 DQWVWKADPSGQYTAKSAYGVLWGEMFEEQQ---DGVFEELWKLKLPSKIT-IFAWRLIR 230
Query: 508 EILPTRMQLKDKGV*-----CPTYCVSYEHQEEDLEHIFFQCPFSVQVW 551
+ LPTR L+ K + CP +C S EE H+FF C VW
Sbjct: 231 DRLPTRSNLRRKQIEVDDPRCP-FCRS---AEESAAHLFFHCSRIAPVW 365
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.343 0.152 0.517
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,602,927
Number of Sequences: 63676
Number of extensions: 438697
Number of successful extensions: 3466
Number of sequences better than 10.0: 74
Number of HSP's better than 10.0 without gapping: 2512
Number of HSP's successfully gapped in prelim test: 95
Number of HSP's that attempted gapping in prelim test: 888
Number of HSP's gapped (non-prelim): 2666
length of query: 555
length of database: 12,639,632
effective HSP length: 102
effective length of query: 453
effective length of database: 6,144,680
effective search space: 2783540040
effective search space used: 2783540040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.5 bits)
S2: 61 (28.1 bits)
Medicago: description of AC144514.8