BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC130802.4 + phase: 0
(634 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC218158 similar to UP|Q7XAF8 (Q7XAF8) Papillar cell-specific pe... 308 4e-84
TC225075 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B p... 307 1e-83
TC208452 similar to UP|Q9FF77 (Q9FF77) Pectinesterase, partial (... 289 2e-78
TC225101 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B p... 288 5e-78
TC217842 similar to UP|PME2_CITSI (O04887) Pectinesterase 2 prec... 286 1e-77
TC203506 similar to UP|Q9FK05 (Q9FK05) Pectinesterase, partial (... 286 1e-77
TC203493 similar to UP|Q9M3B0 (Q9M3B0) Pectinesterase-like prote... 272 4e-73
TC225094 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B p... 256 3e-68
TC217339 homologue to UP|Q8RVX1 (Q8RVX1) Pectin methylesterase p... 253 1e-67
TC231526 similar to UP|PME_MEDSA (Q42920) Pectinesterase precurs... 241 5e-64
TC231406 similar to UP|Q9FF78 (Q9FF78) Pectinesterase, partial (... 238 6e-63
TC230039 homologue to UP|Q9M5J0 (Q9M5J0) Pectin methylesterase i... 231 6e-61
TC222783 weakly similar to UP|Q9SMV9 (Q9SMV9) Pectin methylester... 221 1e-57
TC220076 homologue to UP|PME3_PHAVU (Q43111) Pectinesterase 3 pr... 218 6e-57
TC214438 similar to UP|Q708X4 (Q708X4) Pectin methylesterase (F... 213 3e-55
AW424141 similar to PIR|T52331|T52 pectinesterase (EC 3.1.1.11) ... 210 2e-54
TC211743 similar to UP|PME2_CITSI (O04887) Pectinesterase 2 prec... 209 4e-54
CK606276 205 4e-53
CA851330 similar to PIR|T12995|T12 pectinesterase homolog T21L8.... 199 4e-51
TC210310 similar to UP|PME2_CITSI (O04887) Pectinesterase 2 prec... 190 2e-48
>TC218158 similar to UP|Q7XAF8 (Q7XAF8) Papillar cell-specific pectin
methylesterase-like protein, partial (59%)
Length = 1291
Score = 308 bits (790), Expect = 4e-84
Identities = 156/306 (50%), Positives = 204/306 (65%), Gaps = 6/306 (1%)
Frame = +3
Query: 327 VTVAKDGSGDFKTISEALAAIPQ---TYKGRYVVYVKEGVYDETVTVTKKMVNLTMYGDG 383
VTV++DGSG+F TI++A+AA P + G +++YV GVY+E V++ KK L M GDG
Sbjct: 153 VTVSQDGSGNFTTINDAIAAAPNKSVSTDGYFLIYVTAGVYEENVSIDKKKTYLMMVGDG 332
Query: 384 GLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSI 443
K+IITGN++ VDG TF +A+ V+G GFVG +M RNTAGA+K QAVA R AD S
Sbjct: 333 INKTIITGNRSVVDGWTTFSSATLAVVGQGFVGVNMTIRNTAGAVKHQAVALRSGADLST 512
Query: 444 FVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNII 503
F +C+FEGYQDTLY + RQFY +C I GT+DFIFG+A VFQNC++ R P+ Q N I
Sbjct: 513 FYSCSFEGYQDTLYVHSLRQFYSECDIYGTVDFIFGNAKVVFQNCKMYPRLPMSGQFNAI 692
Query: 504 TANGRIDSKSNTAFVLQKCVIKGEDDLPST--TKNYIGRPWKEYSRTIIMESDIPALIQP 561
TA GR D +T + C I+ DDL ++ Y+GRPWKEYSRT+ M++ + ++I
Sbjct: 693 TAQGRTDPNQDTGISIHNCTIRAADDLAASNGVATYLGRPWKEYSRTVYMQTVMDSVIHA 872
Query: 562 EGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIKRGEALTYTVEPFLDG-SW 620
+GW W+GDFAL TLYY EY N G G+ TD RV W G I +A +TV FL G W
Sbjct: 873 KGWREWDGDFALSTLYYAEYSNSGPGSGTDNRVTWPGYHVINATDAANFTVSNFLLGDDW 1052
Query: 621 INGTGV 626
+ TGV
Sbjct: 1053LPQTGV 1070
>TC225075 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (87%)
Length = 1744
Score = 307 bits (786), Expect = 1e-83
Identities = 163/331 (49%), Positives = 210/331 (63%), Gaps = 5/331 (1%)
Frame = +1
Query: 307 PSWAAPAVDLPGSTEKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDE 366
PSW P TP+VTVA DGSG++ I +A+ A P R+V+ VK+GVY E
Sbjct: 559 PSWIKPKERKLLQAIAVTPDVTVALDGSGNYAKIMDAVLAAPDYSMKRFVILVKKGVYVE 738
Query: 367 TVTVTKKMVNLTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAG 426
V + KK N+ + G G ++I+GN++ VDG TF++A+F V G GF+ RD+ F+NTAG
Sbjct: 739 NVEIKKKKWNIMILGQGMDATVISGNRSVVDGWTTFRSATFAVSGRGFIARDISFQNTAG 918
Query: 427 AIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQ 486
K QAVA R +D S+F C GYQD+LY T RQF+RDC ISGT+D+IFG A+AVFQ
Sbjct: 919 PEKHQAVALRSDSDLSVFFRCGIFGYQDSLYTHTMRQFFRDCTISGTVDYIFGDATAVFQ 1098
Query: 487 NCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDL-PS--TTKNYIGRPWK 543
NC L ++K L NQKN ITA+GR D T F Q C I + DL PS T + Y+GRPWK
Sbjct: 1099NCFLRVKKGLPNQKNTITAHGRKDPNEPTGFSFQFCNITADSDLIPSVGTAQTYLGRPWK 1278
Query: 544 EYSRTIIMESDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIK 603
YSRT+ M+S + +I EGWL W G+FAL TLYY EY N GAGA RVKW G +
Sbjct: 1279SYSRTVFMQSYMSEVIGAEGWLEWNGNFALDTLYYAEYMNTGAGAGVANRVKWPGYHALN 1458
Query: 604 -RGEALTYTVEPFLDGS-WINGTGVPAHLGL 632
+A +TV F++G+ W+ TGV GL
Sbjct: 1459DSSQASNFTVSQFIEGNLWLPSTGVTFTAGL 1551
Score = 32.3 bits (72), Expect = 0.66
Identities = 35/147 (23%), Positives = 64/147 (42%), Gaps = 10/147 (6%)
Frame = +1
Query: 144 AYEDCKQLFEDAKEEMGFSIT--EVGQLDISKLASKEAELNNWLSAVISYQDTCSDGFPE 201
A DC L + + + + ++++ + + + + ++L WLSA +++ +TC +GF E
Sbjct: 250 AIADCLDLLDLSSDVLSWALSASQNPKGKHNSTGNLSSDLRTWLSAALAHPETCMEGF-E 426
Query: 202 GELKKKMEMIFAESRQLLSNSLAVVSQVSQIVNAFQGGLSGFKLP-WGKSD----AHAPA 256
G ++ A Q++S +++QV + F S + P W K A A
Sbjct: 427 GTNSIVKGLVSAGIGQVVSLVEQLLAQVLPAQDQFDAASSKGQFPSWIKPKERKLLQAIA 606
Query: 257 PDADTDAVADDDEDLA---DAPDGAPD 280
D D + A DA APD
Sbjct: 607 VTPDVTVALDGSGNYAKIMDAVLAAPD 687
>TC208452 similar to UP|Q9FF77 (Q9FF77) Pectinesterase, partial (56%)
Length = 1273
Score = 289 bits (740), Expect = 2e-78
Identities = 147/313 (46%), Positives = 200/313 (62%), Gaps = 7/313 (2%)
Frame = +1
Query: 327 VTVAKDGSGDFKTISEALAAIPQTYK---GRYVVYVKEGVYDETVTVTKKMVNLTMYGDG 383
V V+ G ++ +I +A+AA P K G ++VYV+EG+Y+E V + K+ N+ + GDG
Sbjct: 292 VIVSHYGIDNYTSIGDAIAAAPNNTKPEDGYFLVYVREGLYEEYVVIPKEKKNILLVGDG 471
Query: 384 GLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSI 443
K+IITGN + +DG TF +++F V G+ F+ D+ FRNTAG K QAVA R AD S
Sbjct: 472 INKTIITGNHSVIDGWTTFNSSTFAVSGERFIAVDVTFRNTAGPEKHQAVAVRNNADLST 651
Query: 444 FVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNII 503
F C+FEGYQDTLY + RQFYR+C I GT+DFIFG+A+ VFQ C++ RKPL NQKN +
Sbjct: 652 FYRCSFEGYQDTLYVHSLRQFYRECEIYGTVDFIFGNAAVVFQGCKIYARKPLPNQKNAV 831
Query: 504 TANGRIDSKSNTAFVLQKCVIKGEDDLP---STTKNYIGRPWKEYSRTIIMESDIPALIQ 560
TA GR D NT +Q C I DL ++T +++GRPWK YSRT+ ++S I +IQ
Sbjct: 832 TAQGRTDPNQNTGISIQNCSIDAAPDLVADLNSTMSFLGRPWKVYSRTVYLQSYIGNVIQ 1011
Query: 561 PEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIKRGEALTYTVEPF-LDGS 619
P GWL W G L TL+YGE++N G G+ T RV W G + +A +TV F L +
Sbjct: 1012PAGWLEWNGTVGLDTLFYGEFNNYGPGSNTSNRVTWPGYSLLNATQAWNFTVLNFTLGNT 1191
Query: 620 WINGTGVPAHLGL 632
W+ T +P GL
Sbjct: 1192WLPDTDIPYTEGL 1230
>TC225101 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (64%)
Length = 1177
Score = 288 bits (737), Expect = 5e-78
Identities = 150/308 (48%), Positives = 197/308 (63%), Gaps = 8/308 (2%)
Frame = +3
Query: 333 GSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDETVTVTKKMVNLTMYGDGGLKSIITGN 392
G ++ + +A+ A P RYV+++K GVY E V + KK NL M GDG +II+GN
Sbjct: 207 GRENYTKVMDAVLAAPNYSMQRYVIHIKRGVYYENVEIKKKKWNLMMVGDGMDATIISGN 386
Query: 393 KNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSIFVNCNFEGY 452
++F+DG TF++A+F V G GF+ RD+ F+NTAG K QAVA R +D S+F C GY
Sbjct: 387 RSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVALRSDSDLSVFFRCGIFGY 566
Query: 453 QDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNIITANGRIDSK 512
QD+LY T RQFYR+C ISGT+DFIFG A+A+FQNC + +K L NQKN ITA+GR +
Sbjct: 567 QDSLYTHTMRQFYRECKISGTVDFIFGDATAIFQNCHISAKKGLPNQKNTITAHGRKNPD 746
Query: 513 SNTAFVLQKCVIKGEDDLP------STTKNYIGRPWKEYSRTIIMESDIPALIQPEGWLP 566
T F +Q C I + DL ++T Y+GRPWK YSRTI M+S I +++PEGWL
Sbjct: 747 EPTGFSIQFCNISADYDLVNSVNSCNSTHTYLGRPWKPYSRTIFMQSYISDVLRPEGWLE 926
Query: 567 WEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIK-RGEALTYTVEPFLDGS-WINGT 624
W GDFAL TLYY EY N G GA RVKW G + +A +TV F++G+ + T
Sbjct: 927 WNGDFALDTLYYAEYMNYGPGAGVANRVKWQGYHVMNDSSQASNFTVSQFIEGNLCLPST 1106
Query: 625 GVPAHLGL 632
GV GL
Sbjct: 1107GVTFTAGL 1130
>TC217842 similar to UP|PME2_CITSI (O04887) Pectinesterase 2 precursor
(Pectin methylesterase) (PE) , partial (48%)
Length = 1225
Score = 286 bits (733), Expect = 1e-77
Identities = 139/271 (51%), Positives = 180/271 (66%), Gaps = 4/271 (1%)
Frame = +3
Query: 366 ETVTVTKKMVNLTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTA 425
E + + + + NL + GDG +I+TGN N +DG TF++A+F V GDGF+ RD+ F NTA
Sbjct: 9 ENIDIKRTVKNLMIVGDGMGATIVTGNHNAIDGSTTFRSATFAVDGDGFIARDITFENTA 188
Query: 426 GAIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVF 485
G K QAVA R AD S+F C+F GYQDTLY +RQFYRDC I GT+DFIFG A AV
Sbjct: 189 GPQKHQAVALRSGADHSVFYRCSFRGYQDTLYVYANRQFYRDCDIYGTVDFIFGDAVAVL 368
Query: 486 QNCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDLPS---TTKNYIGRPW 542
QNC + +RKP+ NQ+N +TA GR D NT ++ C I DL + + + ++GRPW
Sbjct: 369 QNCNIYVRKPMSNQQNTVTAQGRTDPNENTGIIIHNCRITAAGDLKAVQGSFRTFLGRPW 548
Query: 543 KEYSRTIIMESDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDI 602
++YSRT++M+S + LI P GW PW G+FAL TLYY E+ N GAGA T RV W G + I
Sbjct: 549 QKYSRTVVMKSALDGLISPAGWFPWSGNFALSTLYYAEHANTGAGASTGGRVDWAGFRVI 728
Query: 603 KRGEALTYTVEPFL-DGSWINGTGVPAHLGL 632
EA+ +TV FL GSWI G+GVP GL
Sbjct: 729 SSTEAVKFTVGNFLAGGSWIPGSGVPFDEGL 821
>TC203506 similar to UP|Q9FK05 (Q9FK05) Pectinesterase, partial (57%)
Length = 1320
Score = 286 bits (733), Expect = 1e-77
Identities = 149/313 (47%), Positives = 194/313 (61%), Gaps = 6/313 (1%)
Frame = +2
Query: 326 NVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDET-VTVTKKMVNLTMYGDGG 384
++TV+K G+G KTI+EA+ P+ R+++YV+ G Y+E + V +K N+ GDG
Sbjct: 77 DITVSKTGNGTVKTIAEAIKKAPEHSTRRFIIYVRAGRYEENNLKVGRKKTNVMFIGDGK 256
Query: 385 LKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSIF 444
K++ITG KN +DG+ TF +ASF G GF+ RD+ F N AG K QAVA RV AD ++
Sbjct: 257 GKTVITGKKNVIDGMTTFHSASFAASGAGFIARDITFENYAGPAKHQAVALRVGADHAVV 436
Query: 445 VNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNIIT 504
CN GYQD+ Y ++RQF+R+C I GT+DFIFG+A+ VFQ C + RKP+ QKN IT
Sbjct: 437 YRCNIVGYQDSCYVHSNRQFFRECNIYGTVDFIFGNAAVVFQKCNIYARKPMAQQKNTIT 616
Query: 505 ANGRIDSKSNTAFVLQKCVIKGEDDL---PSTTKNYIGRPWKEYSRTIIMESDIPALIQP 561
A R D NT L C I DL + Y+GRPWK+YSRT+ + S + I P
Sbjct: 617 AQNRKDPNQNTGISLHNCRILPAPDLAPVKGSFPTYLGRPWKQYSRTVYLVSYMGDHIHP 796
Query: 562 EGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIKRG-EALTYTVEPFLDGS- 619
GWL W GDFAL TLYYGEY N G GA RV+W G + IK EA +TV F+ GS
Sbjct: 797 RGWLEWNGDFALNTLYYGEYMNYGPGAAVGQRVQWPGYRVIKSTMEANRFTVAQFISGSA 976
Query: 620 WINGTGVPAHLGL 632
W+ TGV GL
Sbjct: 977 WLPSTGVAFAAGL 1015
>TC203493 similar to UP|Q9M3B0 (Q9M3B0) Pectinesterase-like protein
(At3g49220), partial (59%)
Length = 1298
Score = 272 bits (695), Expect = 4e-73
Identities = 142/314 (45%), Positives = 189/314 (59%), Gaps = 7/314 (2%)
Frame = +1
Query: 326 NVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDE-TVTVTKKMVNLTMYGDGG 384
++ V+KDG+G KTI+EA+ +P+ R ++Y++ G Y+E + + +K N+ GDG
Sbjct: 205 DIVVSKDGNGTVKTIAEAIKKVPEYSSRRIIIYIRAGRYEEDNLKLGRKKTNVMFIGDGK 384
Query: 385 LKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSIF 444
K++ITG +N+ + TF TASF G GF+ +DM F N AG + QAVA RV AD ++
Sbjct: 385 GKTVITGGRNYYQNLTTFHTASFAASGSGFIAKDMTFENYAGPGRHQAVALRVGADHAVV 564
Query: 445 VNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNIIT 504
CN GYQDT+Y ++RQFYR+C I GT+DFIFG+A+ VFQNC L RKP+ QKN IT
Sbjct: 565 YRCNIIGYQDTMYVHSNRQFYRECDIYGTVDFIFGNAAVVFQNCTLWARKPMAQQKNTIT 744
Query: 505 ANGRIDSKSNTAFVLQKCVIKGEDDLPSTT---KNYIGRPWKEYSRTIIMESDIPALIQP 561
A R D NT + C I DL ++ Y+GRPWK Y+RT+ M S I + P
Sbjct: 745 AQNRKDPNQNTGISIHNCRIMATPDLEASKGSYPTYLGRPWKLYARTVFMLSYIGDHVHP 924
Query: 562 EGWLPWE-GDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIKRG-EALTYTVEPFLDG- 618
GWL W FAL T YYGEY N G G+ RV W G + I EA +TV F+ G
Sbjct: 925 RGWLEWNTSSFALDTCYYGEYMNYGPGSALGQRVNWAGYRAINSTVEASRFTVGQFISGS 1104
Query: 619 SWINGTGVPAHLGL 632
SW+ TGV GL
Sbjct: 1105SWLPSTGVAFIAGL 1146
>TC225094 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (47%)
Length = 919
Score = 256 bits (653), Expect = 3e-68
Identities = 130/250 (52%), Positives = 168/250 (67%), Gaps = 5/250 (2%)
Frame = +3
Query: 388 IITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSIFVNC 447
II+GN++ VDG TF++A+F V G GF+ RD+ F+NTAG K QAVA R D S+F C
Sbjct: 3 IISGNRSVVDGWTTFRSATFAVSGRGFIARDISFQNTAGPEKHQAVALRSDTDLSVFFRC 182
Query: 448 NFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNIITANG 507
GYQD+LY T RQF+R+C I+GT+D+IFG A+AVFQNC L ++K L NQKN ITA+G
Sbjct: 183 GIFGYQDSLYTHTMRQFFRECTITGTVDYIFGDATAVFQNCFLRVKKGLPNQKNTITAHG 362
Query: 508 RIDSKSNTAFVLQKCVIKGEDDL---PSTTKNYIGRPWKEYSRTIIMESDIPALIQPEGW 564
R D T F Q C I + DL S+T++Y+GRPWK YSRT+ M+S + +I+ EGW
Sbjct: 363 RKDPNEPTGFSFQFCNITADSDLVPWVSSTQSYLGRPWKSYSRTVFMQSYMSEVIRGEGW 542
Query: 565 LPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIK-RGEALTYTVEPFLDGS-WIN 622
L W G+FAL+TLYYGEY N GAGA RVKW G +A +TV F++G+ W+
Sbjct: 543 LEWNGNFALETLYYGEYMNTGAGAGLANRVKWPGYHPFNDSNQASNFTVAQFIEGNLWLP 722
Query: 623 GTGVPAHLGL 632
TGV GL
Sbjct: 723 STGVTYTAGL 752
>TC217339 homologue to UP|Q8RVX1 (Q8RVX1) Pectin methylesterase precursor,
partial (48%)
Length = 923
Score = 253 bits (647), Expect = 1e-67
Identities = 128/259 (49%), Positives = 170/259 (65%), Gaps = 8/259 (3%)
Frame = +1
Query: 376 NLTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAA 435
N+ + GDG ++ITGN NF+DG TF+TA+ +GDGF+ +D+ F+NTAG K QAVA
Sbjct: 10 NVMLVGDGKDATVITGNLNFIDGTTTFKTATVAAVGDGFIAQDIWFQNTAGPQKHQAVAL 189
Query: 436 RVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKP 495
RV AD S+ C + +QDTLYA ++RQFYRD I+GT+DFIFG+A+ VFQ C LV RKP
Sbjct: 190 RVGADQSVINRCRIDAFQDTLYAHSNRQFYRDSFITGTVDFIFGNAAVVFQKCDLVARKP 369
Query: 496 LDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDLP---STTKNYIGRPWKEYSRTIIME 552
+D Q N++TA GR D NT +Q+C + DL + K ++GRPWK+YSRT++M+
Sbjct: 370 MDKQNNMVTAQGREDPNQNTGTSIQQCNLTPSSDLKPVVGSIKTFLGRPWKKYSRTVVMQ 549
Query: 553 SDIPALIQPEGWLPWEG---DFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIK-RGEAL 608
S + + I P GW W+ DF L+TLYYGEY N G GA T RV W G IK EA
Sbjct: 550 STLDSHIDPTGWAEWDAQSKDF-LQTLYYGEYMNNGPGAGTSKRVNWPGYHIIKTAAEAS 726
Query: 609 TYTVEPFLDGS-WINGTGV 626
+TV + G+ W+ TGV
Sbjct: 727 KFTVAQLIQGNVWLKNTGV 783
>TC231526 similar to UP|PME_MEDSA (Q42920) Pectinesterase precursor (Pectin
methylesterase) (PE) (P65) , partial (56%)
Length = 966
Score = 241 bits (616), Expect = 5e-64
Identities = 121/252 (48%), Positives = 164/252 (65%), Gaps = 9/252 (3%)
Frame = +2
Query: 389 ITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSIFVNCN 448
ITG+KN+VDGV+T+ TA+F V F+ +++GF NTAGA K QAVA RV AD ++F NCN
Sbjct: 8 ITGSKNYVDGVQTYNTATFGVNAANFMAKNIGFENTAGAEKHQAVALRVTADKAVFYNCN 187
Query: 449 FEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNIITANGR 508
+G+QDTLY Q+ RQFYRDC ++GTIDF+FG A AVFQNC+ ++R PL+NQ+ ++TA GR
Sbjct: 188 MDGFQDTLYTQSQRQFYRDCTVTGTIDFVFGDAVAVFQNCKFIVRMPLENQQCLVTAGGR 367
Query: 509 IDSKSNTAFVLQKCVIKGEDDLPSTTKN--YIGRPWKEYSRTIIMESDIPALIQPEGWLP 566
S +A V Q CV GE ++ + T Y+GRPW+ Y++ +IM+S I + PEG++
Sbjct: 368 SKIDSPSALVFQSCVFTGEPNVLALTPKIAYLGRPWRLYAKVVIMDSQIDDIFVPEGYMA 547
Query: 567 WEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIKRGEALTYTVEPFLD-------GS 619
W G T Y E++N G GA T R+ W G K + EA+ Y F S
Sbjct: 548 WMGSAFKDTSTYYEFNNRGPGANTIGRITWPGFKVLNPIEAVEYYPGKFFQIANSTERDS 727
Query: 620 WINGTGVPAHLG 631
WI G+GVP LG
Sbjct: 728 WILGSGVPYSLG 763
>TC231406 similar to UP|Q9FF78 (Q9FF78) Pectinesterase, partial (35%)
Length = 851
Score = 238 bits (607), Expect = 6e-63
Identities = 115/234 (49%), Positives = 150/234 (63%), Gaps = 1/234 (0%)
Frame = +2
Query: 395 FVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSIFVNCNFEGYQD 454
FVDG TF TA+F V G F+ RDMGFRNTAG K+QAVA AD +++ C + +QD
Sbjct: 2 FVDGTPTFSTATFAVFGRNFIARDMGFRNTAGPQKQQAVALMTSADQAVYYRCQIDAFQD 181
Query: 455 TLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNIITANGRIDSKSN 514
+LYA ++RQFYR+C I GT+DFIFG+++ V QNC ++ R P+ Q+N ITA G+ D N
Sbjct: 182 SLYAHSNRQFYRECNIYGTVDFIFGNSAVVLQNCNIMPRVPMQGQQNPITAQGKTDPNMN 361
Query: 515 TAFVLQKCVIKGEDDLPSTTKNYIGRPWKEYSRTIIMESDIPALIQPEGWLPWEGDFALK 574
T +Q C I DL S+ K Y+GRPWK YS + M S + I P GWLPW G+ A
Sbjct: 362 TGISIQNCNITPFGDL-SSVKTYLGRPWKNYSTPVFM*SPMGIFIHPNGWLPWVGNSAPD 538
Query: 575 TLYYGEYDNVGAGAKTDARVKWIGRKDIKRGEALTYTVEPFLDGS-WINGTGVP 627
T++Y E+ NVG GA T RV W G + I R +A +TV+ FL G WI +G P
Sbjct: 539 TIFYAEFQNVGPGASTKNRVNWKGLRVITRKQASMFTVKAFLSGERWITASGAP 700
>TC230039 homologue to UP|Q9M5J0 (Q9M5J0) Pectin methylesterase isoform alpha
(Fragment) , partial (70%)
Length = 913
Score = 231 bits (590), Expect = 6e-61
Identities = 116/253 (45%), Positives = 159/253 (61%), Gaps = 5/253 (1%)
Frame = +1
Query: 304 DAPPSWAAPAVD--LPGSTEKPTPNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKE 361
D P+W + L + + N+ VAKDG+G+F TI+EA+A P + R+V+++K
Sbjct: 154 DGFPTWLSTKDRKLLQAAVNETNFNLLVAKDGTGNFTTIAEAVAVAPNSSATRFVIHIKA 333
Query: 362 GVYDETVTVTKKMVNLTMYGDGGLKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGF 421
G Y E V V +K NL GDG K+++ ++N VDG TFQ+A+ V+GDGF+ + + F
Sbjct: 334 GAYFENVEVIRKKTNLMFVGDGIGKTVVKASRNVVDGWTTFQSATVAVVGDGFIAKGITF 513
Query: 422 RNTAGAIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHA 481
N+AG K QAVA R +D S F C+F YQDTLY + RQFYRDC + GT+DFIFG+A
Sbjct: 514 ENSAGPSKHQAVALRSGSDFSAFYKCSFVAYQDTLYVHSLRQFYRDCDVYGTVDFIFGNA 693
Query: 482 SAVFQNCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDL---PSTTKNYI 538
+ V QNC L RKP +NQ+N+ TA GR D NT + C + DL S KNY+
Sbjct: 694 ATVLQNCNLYARKPNENQRNLFTAQGREDPNQNTGISILNCKVAAAADLIPVKSQFKNYL 873
Query: 539 GRPWKEYSRTIIM 551
GRPWK+YSRT+ +
Sbjct: 874 GRPWKKYSRTVYL 912
>TC222783 weakly similar to UP|Q9SMV9 (Q9SMV9) Pectin methylesterase
precursor , partial (29%)
Length = 630
Score = 221 bits (562), Expect = 1e-57
Identities = 100/183 (54%), Positives = 138/183 (74%)
Frame = +3
Query: 325 PNVTVAKDGSGDFKTISEALAAIPQTYKGRYVVYVKEGVYDETVTVTKKMVNLTMYGDGG 384
P+ TVAKDGSG F T+ +A+ + P+ ++GRY++YVK G+YDE +TV KK NL +YGDG
Sbjct: 81 PHATVAKDGSGQFTTVLDAINSYPKKHQGRYIIYVKAGIYDEYITVDKKKPNLLIYGDGP 260
Query: 385 LKSIITGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSIF 444
K+IITG KNF +G +T +TA+F + + F+ + + F NTAGA QAVA RVQ D S+F
Sbjct: 261 TKTIITGRKNFHEGTKTMRTATFSTVAEDFMAKSIAFENTAGAEGHQAVALRVQGDRSVF 440
Query: 445 VNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNIIT 504
+C GYQDTLYA HRQFYR+C ISGTIDFIFG+++ + QN ++++RKP+ NQ+NI+
Sbjct: 441 FDCAMRGYQDTLYAHAHRQFYRNCEISGTIDFIFGYSTTLIQNSKILVRKPMPNQQNIVV 620
Query: 505 ANG 507
A+G
Sbjct: 621 ADG 629
>TC220076 homologue to UP|PME3_PHAVU (Q43111) Pectinesterase 3 precursor
(Pectin methylesterase 3) (PE 3) , partial (39%)
Length = 808
Score = 218 bits (555), Expect = 6e-57
Identities = 110/222 (49%), Positives = 149/222 (66%), Gaps = 4/222 (1%)
Frame = +2
Query: 409 VLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDC 468
V G GF+ +D+GF N AGA K QAVA R +D S+F C+F G+QDTLYA ++RQFYRDC
Sbjct: 83 VKGKGFIAKDIGFVNNAGASKHQAVAFRSGSDRSVFFRCSFNGFQDTLYAHSNRQFYRDC 262
Query: 469 VISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKG-E 527
I+GTIDFIFG+A+AVFQNC+++ R+PL NQ N ITA G+ D NT ++QK E
Sbjct: 263 DITGTIDFIFGNAAAVFQNCKIMPRQPLPNQFNTITAQGKKDRNQNTGIIIQKSKFTPLE 442
Query: 528 DDLPSTTKNYIGRPWKEYSRTIIMESDIPALIQPEGWLPWEGDF-ALKTLYYGEYDNVGA 586
++L T Y+GRPWK++S T+IM+SDI + ++P GW+ W + + T++Y EY N G
Sbjct: 443 NNL--TAPTYLGRPWKDFSTTVIMQSDIGSFLKPVGWMSWVPNVEPVSTIFYAEYQNTGP 616
Query: 587 GAKTDARVKWIGRK-DIKRGEALTYTVEPFLDG-SWINGTGV 626
GA RVKW G K + GEA +TV+ F+ G W+ V
Sbjct: 617 GADVSQRVKWAGYKPTLTDGEAGKFTVQSFIQGPEWLPNAAV 742
>TC214438 similar to UP|Q708X4 (Q708X4) Pectin methylesterase (Fragment) ,
partial (49%)
Length = 1026
Score = 213 bits (541), Expect = 3e-55
Identities = 108/207 (52%), Positives = 132/207 (63%), Gaps = 5/207 (2%)
Frame = +3
Query: 431 QAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQL 490
QAVA RV D S F NC+ +QDTLY +RQF+ C+I+GT+DFIFG+++ VFQ+C +
Sbjct: 6 QAVALRVGGDLSAFFNCDILAFQDTLYVHNNRQFFEKCLIAGTVDFIFGNSAVVFQDCDI 185
Query: 491 VLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDLPSTTKN---YIGRPWKEYSR 547
R P QKN++TA GR+D NT V+QKC I +DL S KN Y+GRPWKEYSR
Sbjct: 186 HARLPSSGQKNMVTAQGRVDPNQNTGIVIQKCRIGATNDLESVKKNFKTYLGRPWKEYSR 365
Query: 548 TIIMESDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDI-KRGE 606
T+IM+S I +I P GW W G+FAL TL Y EY N G GA T RV W G K I E
Sbjct: 366 TVIMQSSISDVIDPIGWHEWSGNFALSTLVYREYQNTGPGAGTSNRVTWKGYKVITDAAE 545
Query: 607 ALTYTVEPFLDG-SWINGTGVPAHLGL 632
A YT F+ G SW+ TG P LGL
Sbjct: 546 ARDYTPGSFIGGSSWLGSTGFPFSLGL 626
>AW424141 similar to PIR|T52331|T52 pectinesterase (EC 3.1.1.11) [imported] -
Salix gilgiana, partial (22%)
Length = 418
Score = 210 bits (534), Expect = 2e-54
Identities = 102/137 (74%), Positives = 114/137 (82%), Gaps = 3/137 (2%)
Frame = +2
Query: 429 KEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNC 488
K QAVAARVQAD +IF NC FEGYQDTLYAQTHRQFYRDC ISGTIDFIFG ASAVFQNC
Sbjct: 8 KHQAVAARVQADRAIFFNCAFEGYQDTLYAQTHRQFYRDCYISGTIDFIFGDASAVFQNC 187
Query: 489 QLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDD---LPSTTKNYIGRPWKEY 545
+V+RKPL+NQ+NI+TA GR+D + NT FVLQKCVIK + D L T KNY+GRPWKEY
Sbjct: 188 TMVVRKPLENQQNIVTAQGRLDKQENTGFVLQKCVIKADTDLVPLKDTIKNYLGRPWKEY 367
Query: 546 SRTIIMESDIPALIQPE 562
SRTIIME+ I LI P+
Sbjct: 368 SRTIIMETQIDDLIHPD 418
>TC211743 similar to UP|PME2_CITSI (O04887) Pectinesterase 2 precursor
(Pectin methylesterase) (PE) , partial (39%)
Length = 601
Score = 209 bits (531), Expect = 4e-54
Identities = 103/201 (51%), Positives = 131/201 (64%), Gaps = 1/201 (0%)
Frame = +2
Query: 417 RDMGFRNTAGAIKEQAVAARVQADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDF 476
+D+ FRNTAGA QAVA R +D S+F C+FEGYQDTLY + RQFYR+C I GT+DF
Sbjct: 2 QDITFRNTAGATNHQAVALRSGSDLSVFYRCSFEGYQDTLYVYSDRQFYRECDIYGTVDF 181
Query: 477 IFGHASAVFQNCQLVLRKPLDNQKNIITANGRIDSKSNTAFVLQKCVIKGEDDLPSTTKN 536
IFG+A+ VFQNC + R P N+ N ITA GR D NT + + DL +
Sbjct: 182 IFGNAAVVFQNCNIYARNP-PNKVNTITAQGRTDPNQNTGISIHNSKVTAASDLMG-VRT 355
Query: 537 YIGRPWKEYSRTIIMESDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKW 596
Y+GRPW++YSRT+ M++ + +LI PEGWL W G+FAL TLYYGEY N G G+ T RV W
Sbjct: 356 YLGRPWQQYSRTVFMKTYLDSLINPEGWLEWSGNFALSTLYYGEYMNTGPGSSTANRVNW 535
Query: 597 IGRKDI-KRGEALTYTVEPFL 616
+G I EA +TV F+
Sbjct: 536 LGYHVITSASEASKFTVGNFI 598
>CK606276
Length = 751
Score = 205 bits (522), Expect = 4e-53
Identities = 103/201 (51%), Positives = 124/201 (61%), Gaps = 7/201 (3%)
Frame = +1
Query: 439 ADCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDN 498
AD S F NC+FEGYQDTLY + RQFY+ C I GT+DFIFG+A+A+ Q+C + R P+ N
Sbjct: 10 ADMSTFYNCSFEGYQDTLYVHSLRQFYKSCDIYGTVDFIFGNAAALLQDCNMYPRLPMQN 189
Query: 499 QKNIITANGRIDSKSNTAFVLQKCVIKGEDDLPSTTKN------YIGRPWKEYSRTIIME 552
Q N ITA GR D NT +Q C I DL T N Y+GRPWKEYSRT+ M+
Sbjct: 190 QFNAITAQGRTDPNQNTGISIQNCCIIAASDLGDATNNYNGIKTYLGRPWKEYSRTVYMQ 369
Query: 553 SDIPALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDIKRGEALTYTV 612
S I LI P+GW W GDFAL TLYY E+ N G G+ T RV W G I +A +TV
Sbjct: 370 SFIDGLIDPKGWNEWSGDFALSTLYYAEFANWGPGSNTSNRVAWEGYHLIDEKDADDFTV 549
Query: 613 EPFLDG-SWINGTGVPAHLGL 632
F+ G W+ TGVP GL
Sbjct: 550 HKFIQGEKWLPQTGVPFXAGL 612
>CA851330 similar to PIR|T12995|T12 pectinesterase homolog T21L8.150 -
Arabidopsis thaliana, partial (37%)
Length = 700
Score = 199 bits (505), Expect = 4e-51
Identities = 102/227 (44%), Positives = 142/227 (61%), Gaps = 4/227 (1%)
Frame = +2
Query: 331 KDGSGDFKTISEAL-AAIPQTYKGRYVVYVKEGVYDETVTVTKKMVNLTMYGDGGLKSII 389
K GSG+FKT+ +AL AA + K R+V++VK+GVY E + V N+ + GDG +II
Sbjct: 8 KXGSGNFKTVQDALNAAAKRKEKTRFVIHVKKGVYRENIEVALHNDNIMLVGDGLRNTII 187
Query: 390 TGNKNFVDGVRTFQTASFVVLGDGFVGRDMGFRNTAGAIKEQAVAARVQADCSIFVNCNF 449
T ++ DG T+ +A+ + G F+ RD+ F+N+AG K QAVA R +D S+F C
Sbjct: 188 TSARSVQDGYTTYSSATAGIDGLHFIARDITFQNSAGVHKGQAVALRSASDLSVFYRCGI 367
Query: 450 EGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQKNIITANGRI 509
GYQDTL A RQFYR C I GT+DFIFG+A+ VFQNC + R+PL+ Q N+ITA GR
Sbjct: 368 MGYQDTLMAHAQRQFYRQCYIYGTVDFIFGNAAVVFQNCYIFARRPLEGQANMITAQGRG 547
Query: 510 DSKSNTAFVLQKCVIKGEDDLPSTTKNY---IGRPWKEYSRTIIMES 553
D NT + I+ DL Y +GRPW++YSR ++M++
Sbjct: 548 DPFQNTGISIHNSQIRAAPDLKPVVDKYNTFLGRPWQQYSRVVVMKT 688
>TC210310 similar to UP|PME2_CITSI (O04887) Pectinesterase 2 precursor
(Pectin methylesterase) (PE) , partial (38%)
Length = 678
Score = 190 bits (482), Expect = 2e-48
Identities = 93/193 (48%), Positives = 122/193 (63%), Gaps = 5/193 (2%)
Frame = +1
Query: 440 DCSIFVNCNFEGYQDTLYAQTHRQFYRDCVISGTIDFIFGHASAVFQNCQLVLRKPLDNQ 499
D S+F C+FEGYQDTLY + RQFYR+C I GT+DFIFG+A+ V QNC + R P N+
Sbjct: 1 DLSVFYKCSFEGYQDTLYVHSERQFYRECNIYGTVDFIFGNAAVVLQNCNIFARNP-PNK 177
Query: 500 KNIITANGRIDSKSNTAFVLQKCVIKGEDDL---PSTTKNYIGRPWKEYSRTIIMESDIP 556
N ITA GR D NT + + DL ++ + Y+GRPWK+YSRT+ M++ +
Sbjct: 178 VNTITAQGRTDPNQNTGISIHNSRVTAASDLRPVQNSVRTYLGRPWKQYSRTVFMKTYLD 357
Query: 557 ALIQPEGWLPWEGDFALKTLYYGEYDNVGAGAKTDARVKWIGRKDI-KRGEALTYTVEPF 615
LI P GW+ W G+FAL TLYYGEY N G G+ T RVKW G + I EA ++V F
Sbjct: 358 GLINPAGWMEWSGNFALDTLYYGEYMNTGPGSSTARRVKWSGYRVITSASEASKFSVANF 537
Query: 616 LDG-SWINGTGVP 627
+ G +W+ T VP
Sbjct: 538 IAGNAWLPSTKVP 576
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.316 0.134 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,555,715
Number of Sequences: 63676
Number of extensions: 322329
Number of successful extensions: 2054
Number of sequences better than 10.0: 148
Number of HSP's better than 10.0 without gapping: 1922
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1981
length of query: 634
length of database: 12,639,632
effective HSP length: 103
effective length of query: 531
effective length of database: 6,081,004
effective search space: 3229013124
effective search space used: 3229013124
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 62 (28.5 bits)
Medicago: description of AC130802.4