BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC125481.10 + phase: 0 /pseudo
(116 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC212040 similar to UP|Q9FNL8 (Q9FNL8) Peptide transporter, part... 123 1e-29
BI787336 similar to PIR|T47573|T47 peptide transport-like protei... 107 8e-25
TC213589 weakly similar to UP|Q8RX67 (Q8RX67) At1g72130/F28P5.1,... 103 1e-23
AW707240 89 4e-19
TC221656 similar to UP|Q9SVS9 (Q9SVS9) Peptide transporter-like ... 86 3e-18
TC233468 similar to UP|O81393 (O81393) Nitrate transporter NTL1,... 75 3e-15
BM177061 similar to GP|20147231|gb At1g68570/F24J5_7 {Arabidopsi... 70 1e-13
TC208340 UP|Q9FRU5 (Q9FRU5) Nitrate transporter NRT1-2, partial ... 69 2e-13
TC233014 similar to UP|Q96400 (Q96400) Nitrite transporter, part... 69 2e-13
CA784302 similar to GP|4102839|gb| LeOPT1 {Lycopersicon esculent... 51 1e-12
BE661196 64 1e-11
AW233936 similar to PIR|T47573|T47 peptide transport-like protei... 62 3e-11
TC230623 UP|Q9FRU2 (Q9FRU2) Nitrate transporter NRT1-5 (Fragment... 62 4e-11
BI316774 55 4e-09
BM084986 50 1e-07
BU551277 47 2e-06
TC223303 similar to UP|Q8RV26 (Q8RV26) Oligopeptide transporter-... 39 3e-04
BU965567 35 0.005
BU763225 28 0.63
TC216341 similar to UP|Q6K4D4 (Q6K4D4) Proliferating-cell nucleo... 27 1.1
>TC212040 similar to UP|Q9FNL8 (Q9FNL8) Peptide transporter, partial (29%)
Length = 765
Score = 123 bits (309), Expect = 1e-29
Identities = 57/100 (57%), Positives = 75/100 (75%)
Frame = +1
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
T DG V+ KG+P+LRS SGGW+A +F++ EV ER AYYGI+SNLI YLT L Q T ++
Sbjct: 160 TQDGTVNIKGKPILRSKSGGWKACSFVVVYEVFERMAYYGISSNLILYLTTKLHQGTVSS 339
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYI 113
A NV W GT + P+LGA++AD+FLGRY T +IAS +Y+
Sbjct: 340 ANNVTNWVGTIWMTPILGAYVADAFLGRYWTFVIASTIYL 459
>BI787336 similar to PIR|T47573|T47 peptide transport-like protein -
Arabidopsis thaliana, partial (18%)
Length = 421
Score = 107 bits (267), Expect = 8e-25
Identities = 48/99 (48%), Positives = 69/99 (69%)
Frame = +3
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
T DG VD+ G P + +G W+A +I+ E ER AYYG+++NL+ Y L Q +ATA
Sbjct: 120 TKDGTVDYCGNPANKKETGTWKACPYILGNECCERLAYYGMSTNLVLYFKNRLNQHSATA 299
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVY 112
++NV+ WSGT + PL+GA+LADS+LGRY TI + S++Y
Sbjct: 300 SKNVSNWSGTCYITPLIGAYLADSYLGRYWTIAVFSIIY 416
>TC213589 weakly similar to UP|Q8RX67 (Q8RX67) At1g72130/F28P5.1, partial
(16%)
Length = 683
Score = 103 bits (257), Expect = 1e-23
Identities = 48/81 (59%), Positives = 61/81 (75%)
Frame = +3
Query: 32 GGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLG 91
GGW AA FII VE AERFAY G+ SNLI YLT L + AA++VN W G +SL PLLG
Sbjct: 84 GGWNAAIFIIFVEFAERFAYQGLASNLIQYLTNVLNEPITQAAKDVNTWVGASSLFPLLG 263
Query: 92 AFLADSFLGRYRTIIIASLVY 112
F+ADS+LGR+ TI+++S++Y
Sbjct: 264 GFIADSYLGRFNTILLSSVIY 326
>AW707240
Length = 411
Score = 88.6 bits (218), Expect = 4e-19
Identities = 48/98 (48%), Positives = 64/98 (64%), Gaps = 1/98 (1%)
Frame = +3
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
TV V++KG P RS +GGW AA I+ +E+ ER + GI NL+ Y+ + ++TA
Sbjct: 102 TVADAVNYKGFPADRSKTGGWVPAALILGIEIVERLSTMGIAVNLVTYMISIMHLPSSTA 281
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTI-IIASL 110
A V + GT+ LL LLG FLADSFLGRY+TI I AS+
Sbjct: 282 ANTVTDFMGTSFLLCLLGGFLADSFLGRYKTIGIFASI 395
>TC221656 similar to UP|Q9SVS9 (Q9SVS9) Peptide transporter-like protein,
partial (31%)
Length = 860
Score = 85.9 bits (211), Expect = 3e-18
Identities = 40/101 (39%), Positives = 61/101 (59%)
Frame = +3
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATA 73
T DG +D G P +R +G W AA I+ + A++G+ NL+ +LT +GQ A A
Sbjct: 144 TSDGAIDSHGHPAVRKKTGDWVAAILILVNQGLATLAFFGVGVNLVLFLTRVMGQDNAEA 323
Query: 74 AENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
A +V+ W+GT LLGAFL+DS+ GRY T I +++++
Sbjct: 324 ANSVSKWTGTVYHFSLLGAFLSDSYWGRYMTCAIFQVIFVI 446
>TC233468 similar to UP|O81393 (O81393) Nitrate transporter NTL1, partial
(17%)
Length = 975
Score = 75.5 bits (184), Expect = 3e-15
Identities = 38/100 (38%), Positives = 62/100 (62%)
Frame = +3
Query: 16 DGVVDFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAE 75
+G V+++ +P L +GG AA+F++ VE+ E A+ SNL+ YL + S + +A
Sbjct: 45 EGYVNWRNKPALSGCNGGMLAASFVLVVEILENLAFLANASNLVLYLRQYMHMSPSKSAN 224
Query: 76 NVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYILV 115
NV + GTA LL LLG FL+D+F Y+ +I++++ LV
Sbjct: 225 NVTNFMGTAFLLALLGGFLSDAFFTTYQIYLISAVIEFLV 344
>BM177061 similar to GP|20147231|gb At1g68570/F24J5_7 {Arabidopsis thaliana},
partial (21%)
Length = 396
Score = 70.1 bits (170), Expect = 1e-13
Identities = 33/70 (47%), Positives = 47/70 (67%)
Frame = +3
Query: 45 VAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADSFLGRYRT 104
V E+ A G N+N+I+YLT L AA + + GTASL PLLGAF+ADS+ G++ T
Sbjct: 3 VCEKLAVVGFNTNIISYLTTQLHMPLTKAANTLTNFGGTASLTPLLGAFIADSYAGKFWT 182
Query: 105 IIIASLVYIL 114
+ +AS++Y L
Sbjct: 183 VTLASIIYFL 212
>TC208340 UP|Q9FRU5 (Q9FRU5) Nitrate transporter NRT1-2, partial (98%)
Length = 2068
Score = 69.3 bits (168), Expect = 2e-13
Identities = 38/86 (44%), Positives = 53/86 (61%), Gaps = 3/86 (3%)
Frame = +3
Query: 33 GWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGA 92
GW+ FII E E+ G +NL+ YLT S+ TA +NI++G+ASL LLGA
Sbjct: 54 GWKVMPFIIGNETFEKLGTIGTLANLLVYLTTVFNLSSLTATNIINIFTGSASLSTLLGA 233
Query: 93 FLADSFLGRYRTI---IIASLVYILV 115
FL D++ GRY+T+ IAS + +LV
Sbjct: 234 FLCDTYFGRYKTLGFCTIASFLGLLV 311
>TC233014 similar to UP|Q96400 (Q96400) Nitrite transporter, partial (14%)
Length = 647
Score = 69.3 bits (168), Expect = 2e-13
Identities = 36/84 (42%), Positives = 50/84 (58%)
Frame = +2
Query: 32 GGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLG 91
GG R FI++ EV +R A G + NLI YLT L A+ + + GT+S PL+G
Sbjct: 77 GGIRTLPFILANEVCDRSASAGFHGNLIRYLTQELNMPLVAASNTLTNYGGTSSFTPLIG 256
Query: 92 AFLADSFLGRYRTIIIASLVYILV 115
A +ADS GR+ T+ +A L+Y LV
Sbjct: 257 AIIADSLAGRFWTMTVA*LIYELV 328
>CA784302 similar to GP|4102839|gb| LeOPT1 {Lycopersicon esculentum}, partial
(22%)
Length = 436
Score = 51.2 bits (121), Expect(2) = 1e-12
Identities = 22/47 (46%), Positives = 32/47 (67%)
Frame = +3
Query: 68 QSTATAAENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
+ ++A N++IW GT+ L PL+GA LAD + GRY TI + S VY +
Sbjct: 126 EGNVSSARNISIWLGTSYLTPLIGAVLADGYWGRYWTIAVFSAVYFI 266
Score = 36.2 bits (82), Expect(2) = 1e-12
Identities = 14/28 (50%), Positives = 20/28 (71%)
Frame = +1
Query: 14 TVDGVVDFKGRPVLRSSSGGWRAAAFII 41
T +G V+F+G PVL+ +G WRA FI+
Sbjct: 34 TGEGSVNFRGEPVLKKDTGNWRACPFIL 117
>BE661196
Length = 609
Score = 63.5 bits (153), Expect = 1e-11
Identities = 34/92 (36%), Positives = 53/92 (56%)
Frame = +1
Query: 20 DFKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNI 79
D++GRP + GG AAF++ ++ E A + +NLI Y+T + + AA V
Sbjct: 22 DWRGRPSNPAKHGGMIPAAFVLGLQAFEIMAIAAVGNNLITYVTSEMHFPLSKAANLVTN 201
Query: 80 WSGTASLLPLLGAFLADSFLGRYRTIIIASLV 111
+ GT LL LLG +L+DS+LG + T++I V
Sbjct: 202 FVGTIFLLALLGGYLSDSYLGSFWTMLIFGFV 297
>AW233936 similar to PIR|T47573|T47 peptide transport-like protein -
Arabidopsis thaliana, partial (22%)
Length = 407
Score = 62.4 bits (150), Expect = 3e-11
Identities = 29/47 (61%), Positives = 35/47 (73%)
Frame = +1
Query: 68 QSTATAAENVNIWSGTASLLPLLGAFLADSFLGRYRTIIIASLVYIL 114
Q ATAA NV WSGT + PL+GAFLADS+LGRY TI S+VY++
Sbjct: 31 QGNATAANNVTTWSGTCYITPLIGAFLADSYLGRYWTISSFSIVYVI 171
>TC230623 UP|Q9FRU2 (Q9FRU2) Nitrate transporter NRT1-5 (Fragment), complete
Length = 1873
Score = 62.0 bits (149), Expect = 4e-11
Identities = 29/73 (39%), Positives = 43/73 (58%)
Frame = +3
Query: 33 GWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGA 92
GW+A FII E E+ G +NL+ YLT TA +NI++G+ + +GA
Sbjct: 30 GWKAMPFIIGNETFEKLGAIGTLANLLVYLTTVFNLKNITATNIINIFNGSTNFATFIGA 209
Query: 93 FLADSFLGRYRTI 105
FL+D++ GRY+TI
Sbjct: 210 FLSDTYFGRYKTI 248
>BI316774
Length = 282
Score = 55.5 bits (132), Expect = 4e-09
Identities = 29/70 (41%), Positives = 38/70 (53%)
Frame = +1
Query: 32 GGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLG 91
GG R FII E E+ A YGI N+I YL S A + W+ + +L + G
Sbjct: 73 GGLRTMPFIIVNESLEKVASYGIMPNMILYLRDEYRMSIAKGTSVIYTWTAASDILSIFG 252
Query: 92 AFLADSFLGR 101
AFL+DS+LGR
Sbjct: 253 AFLSDSYLGR 282
>BM084986
Length = 437
Score = 50.4 bits (119), Expect = 1e-07
Identities = 29/83 (34%), Positives = 44/83 (52%)
Frame = +3
Query: 32 GGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLG 91
GG FII+ E R A G+ N+I YL G A A + + T++ P++G
Sbjct: 84 GGLVTMPFIIANEALARVASLGLLPNMILYLMGTYRLHLAQATQILLSSHATSNFTPVVG 263
Query: 92 AFLADSFLGRYRTIIIASLVYIL 114
AF+ADS+LGR+ + + S + L
Sbjct: 264 AFIADSYLGRFLAVGLGSAITFL 332
>BU551277
Length = 560
Score = 46.6 bits (109), Expect = 2e-06
Identities = 23/76 (30%), Positives = 40/76 (52%)
Frame = -2
Query: 40 IISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLLPLLGAFLADSFL 99
I + E + A G+ N++ YL G A + + W + P++GAF+AD++L
Sbjct: 385 IAANEALAKLASVGLMPNMVXYLIGDYRLRVLKATKIMFYWFAATNFAPVIGAFVADAYL 206
Query: 100 GRYRTIIIASLVYILV 115
GR+ I + S++ LV
Sbjct: 205 GRFLAIGLGSILSFLV 158
>TC223303 similar to UP|Q8RV26 (Q8RV26) Oligopeptide transporter-like
protein, partial (20%)
Length = 432
Score = 39.3 bits (90), Expect = 3e-04
Identities = 16/20 (80%), Positives = 20/20 (100%)
Frame = +1
Query: 95 ADSFLGRYRTIIIASLVYIL 114
ADSFLGRYRTI++ASL+Y+L
Sbjct: 1 ADSFLGRYRTIVLASLIYVL 60
>BU965567
Length = 455
Score = 35.0 bits (79), Expect = 0.005
Identities = 22/66 (33%), Positives = 30/66 (45%)
Frame = +3
Query: 28 RSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGPLGQSTATAAENVNIWSGTASLL 87
R GG FII+ E R A G+ N+I YL G A + + + T + +
Sbjct: 258 RCGKGGLVTMPFIIANEALARVATLGLLPNMILYLMGNYNLHLGKATKILLLSVATTNFM 437
Query: 88 PLLGAF 93
PL GAF
Sbjct: 438 PLPGAF 455
>BU763225
Length = 426
Score = 28.1 bits (61), Expect = 0.63
Identities = 28/109 (25%), Positives = 44/109 (39%), Gaps = 17/109 (15%)
Frame = +2
Query: 21 FKGRPVLRSSSGGWRAAAFIISVEVAERFAYYGINSNLINYLTGP-----LGQSTATAAE 75
F G+ VL SG F + +E A + N+ ++ +LT P L + T ++
Sbjct: 5 FSGKRVLEVGSGHGLPGIFAL-LEGAAAVHFQDFNAEVLRFLTIPNLNANLSRDTQLSSF 181
Query: 76 NVNI------------WSGTASLLPLLGAFLADSFLGRYRTIIIASLVY 112
N I WSG LLP + ++ Y I++A VY
Sbjct: 182 NSTICDKAEVCFFAGDWSGIDKLLPHVSTDAKNNQGDGYDFILMAETVY 328
>TC216341 similar to UP|Q6K4D4 (Q6K4D4) Proliferating-cell nucleolar
protein-like, partial (25%)
Length = 1234
Score = 27.3 bits (59), Expect = 1.1
Identities = 15/40 (37%), Positives = 20/40 (49%)
Frame = +3
Query: 63 TGPLGQSTATAAENVNIWSGTASLLPLLGAFLADSFLGRY 102
TGPL +T A + W G ASL ++GA L R+
Sbjct: 972 TGPLQVDDSTIA--IGCWKGRASLTIMVGALECQELLERF 1085
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.323 0.139 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,803,460
Number of Sequences: 63676
Number of extensions: 53380
Number of successful extensions: 218
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 218
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of query: 116
length of database: 12,639,632
effective HSP length: 92
effective length of query: 24
effective length of database: 6,781,440
effective search space: 162754560
effective search space used: 162754560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC125481.10