BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0365.17
(568 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
VML2_HUMAN (Q8NFU0) Bestrophin 3 (Vitelliform macular dystrophy ... 34 0.89
VML1_HUMAN (Q8NFU1) Bestrophin 2 (Vitelliform macular dystrophy ... 32 3.4
SPCN_CHICK (P07751) Spectrin alpha chain, brain (Spectrin, non-e... 32 5.8
LX1B_MOUSE (O35936) Arachidonate 15-lipoxygenase, type II (EC 1.... 32 5.8
5HT1_APLCA (Q16950) 5-hydroxytryptamine 1 receptor (5-HTB1) (Ser... 32 5.8
ERG4_YEAST (P25340) Delta(24(24(1)))-sterol reductase (EC 1.3.1.... 31 7.6
VML1_MOUSE (Q8BGM5) Bestrophin 2 (Vitelliform macular dystrophy ... 31 9.9
>VML2_HUMAN (Q8NFU0) Bestrophin 3 (Vitelliform macular dystrophy
2-like protein 2)
Length = 473
Score = 34.3 bits (77), Expect = 0.89
Identities = 17/48 (35%), Positives = 23/48 (47%), Gaps = 3/48 (6%)
Query: 477 YNKRLLNLSPFTYAPSYYATNAFKAWWSEYWAQISKPLVDCLQCMTDA 524
Y R +L P ++ +Y T WWS+Y S PL D L C+ A
Sbjct: 68 YCNRSADLIPLSFVLGFYVTLVVNRWWSQY---TSIPLPDQLMCVISA 112
>VML1_HUMAN (Q8NFU1) Bestrophin 2 (Vitelliform macular dystrophy
2-like protein 1)
Length = 509
Score = 32.3 bits (72), Expect = 3.4
Identities = 14/45 (31%), Positives = 22/45 (48%), Gaps = 3/45 (6%)
Query: 477 YNKRLLNLSPFTYAPSYYATNAFKAWWSEYWAQISKPLVDCLQCM 521
Y + +L P ++ +Y T WWS+Y + PL D L C+
Sbjct: 68 YCDQYASLIPVSFVLGFYVTLVVNRWWSQY---LCMPLPDALMCV 109
>SPCN_CHICK (P07751) Spectrin alpha chain, brain (Spectrin,
non-erythroid alpha chain) (Fodrin alpha chain)
Length = 2477
Score = 31.6 bits (70), Expect = 5.8
Identities = 28/99 (28%), Positives = 43/99 (43%), Gaps = 15/99 (15%)
Query: 288 LNEAVEDIRNTKTVSLNAAGPLWLFQLWLNAVFESLL-PVQDNPLTVSNTRIDAHRLETL 346
L EA E R + + L A F W E L PV+ N L ++ L
Sbjct: 2084 LLEAQEHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSL---------EEIKAL 2134
Query: 347 TPAYDA-----SSFEADFRKYFTMFLELKHYRSSFSPYS 380
A+DA SS +ADF + + ++K +R + +PY+
Sbjct: 2135 REAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYT 2173
>LX1B_MOUSE (O35936) Arachidonate 15-lipoxygenase, type II (EC
1.13.11.33) (15-LOX-2) (8S-lipoxygenase) (8S-LOX)
Length = 677
Score = 31.6 bits (70), Expect = 5.8
Identities = 30/95 (31%), Positives = 43/95 (44%), Gaps = 3/95 (3%)
Query: 176 FGMITPTLLDVAAITG-LWPIGDDYHSAPAPVKPISIPTDNVSFSRFIKDHYVENGEVSD 234
F TLL ++ +G L PI P P PI +P+D+ ++ + +V N E
Sbjct: 310 FSAAPMTLLHQSSGSGPLLPIAIQLKQTPGPDNPIFLPSDD-TWDWLLAKTWVRNSEFYI 368
Query: 235 AEHVAFLLY-WLSAYVFCTKSLRIPAKLLPLANLL 268
E V LL+ L VF +LR + PL LL
Sbjct: 369 HEAVTHLLHAHLIPEVFALATLRQLPRCHPLFKLL 403
>5HT1_APLCA (Q16950) 5-hydroxytryptamine 1 receptor (5-HTB1)
(Serotonin receptor 1)
Length = 453
Score = 31.6 bits (70), Expect = 5.8
Identities = 47/198 (23%), Positives = 83/198 (41%), Gaps = 28/198 (14%)
Query: 229 NGEVSDAEHVA-FLLYWLSAYVFCTKSLRIPAKLLPLAN-----LLHEGRQLAMARLVLG 282
+ + S+A+HV F +++ + ++ +P L+ L N L+ + Q+ + L +
Sbjct: 215 DNQKSNAKHVCLFDVHFTYSVIYSFICFYVPCTLM-LTNYLRLFLIAQTHQVRIRSLQMT 273
Query: 283 NLYQMLNEAVEDIRNTKTVSLNAAGPL----------WLFQLWLN---AVFESLLPVQDN 329
N Q+ + RN T AA L WL +N A E L+P++
Sbjct: 274 NPPQLRGQGASSYRNQGTQGSKAARTLTIITGTFLACWLPFFIINPIAAADEHLIPLECF 333
Query: 330 PLTVSNTRIDAHRLETLTPAYDASSFEADFRKYFTMFLELKHYRS---SFSPYSKAIRGP 386
+T+ ++ ++ P +S + FR F L + +S S SP S A R
Sbjct: 334 MVTIWLGYFNS----SVNPIIYGTS-NSKFRAAFKRLLRCRSVKSVVGSISPVSPAYRAF 388
Query: 387 FWLRNSYPNASDSALPKE 404
W+R S + S S P +
Sbjct: 389 SWIRPSRLDLSSSEHPSD 406
>ERG4_YEAST (P25340) Delta(24(24(1)))-sterol reductase (EC 1.3.1.71)
(Sterol delta(24(28))-reductase) (C-24(28) sterol
reductase)
Length = 473
Score = 31.2 bits (69), Expect = 7.6
Identities = 26/97 (26%), Positives = 42/97 (42%), Gaps = 11/97 (11%)
Query: 192 LWPIGDDYHSAPAPVKPISIPTDNVSFSRFIKDHY---VENGEVSDAEHVAFLLYWLSAY 248
+W + YH +++P S+ FIK Y +ENG + FL +W+
Sbjct: 60 MWICAEFYHGK------VALPKAGESWMHFIKHLYQLVLENGIPEKYDWTIFLTFWVFQI 113
Query: 249 VFCTKSLRIPAKLLPLANLLHEGRQLAMARLVLGNLY 285
+F I K PL++L +G+QL + LY
Sbjct: 114 IFYYTLPGIWTKGQPLSHL--KGKQLPYFCNAMWTLY 148
>VML1_MOUSE (Q8BGM5) Bestrophin 2 (Vitelliform macular dystrophy
2-like protein 1)
Length = 508
Score = 30.8 bits (68), Expect = 9.9
Identities = 13/45 (28%), Positives = 22/45 (48%), Gaps = 3/45 (6%)
Query: 477 YNKRLLNLSPFTYAPSYYATNAFKAWWSEYWAQISKPLVDCLQCM 521
Y + +L P ++ +Y T WW++Y + PL D L C+
Sbjct: 68 YCDQYASLIPVSFVLGFYVTLVVHRWWNQY---LCMPLPDALMCI 109
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.136 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,016,176
Number of Sequences: 164201
Number of extensions: 2934439
Number of successful extensions: 6440
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 6438
Number of HSP's gapped (non-prelim): 9
length of query: 568
length of database: 59,974,054
effective HSP length: 116
effective length of query: 452
effective length of database: 40,926,738
effective search space: 18498885576
effective search space used: 18498885576
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 68 (30.8 bits)
Lotus: description of TM0365.17