BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0282.12
(484 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UFOG_PETHY (Q43716) Flavonol 3-O-glucosyltransferase (EC 2.4.1.9... 219 9e-57
MGT_STRLI (Q54387) Macrolide glycosyltransferase (EC 2.4.1.-) 42 0.004
OLED_STRAT (Q53685) Oleandomycin glycosyltransferase (EC 2.4.1.-) 40 0.010
HQGT_RAUSE (Q9AR73) Hydroquinone glucosyltransferase (EC 2.4.1.2... 40 0.010
ZOX_PHAVU (P56725) Zeatin O-xylosyltransferase (EC 2.4.2.40) (Ze... 34 0.74
UFO2_MANES (Q40285) Flavonol 3-O-glucosyltransferase 2 (EC 2.4.1... 34 0.74
HQGT_ARATH (Q9M156) Probable hydroquinone glucosyltransferase (E... 34 0.96
ITB8_RABIT (P26013) Integrin beta-8 precursor 33 1.3
UFO1_MANES (Q40284) Flavonol 3-O-glucosyltransferase 1 (EC 2.4.1... 32 4.8
CRTX_ERWHE (Q01330) Zeaxanthin glucosyl transferase (EC 2.4.1.-) 32 4.8
RHO2_SCHPO (Q10133) Rho2 protein 31 8.2
>UFOG_PETHY (Q43716) Flavonol 3-O-glucosyltransferase (EC 2.4.1.91)
(UDP-glucose flavonoid 3-O-glucosyltransferase)
(Anthocyanin rhamnosyl transferase)
Length = 473
Score = 219 bits (559), Expect = 9e-57
Identities = 127/314 (40%), Positives = 188/314 (59%), Gaps = 19/314 (6%)
Query: 12 SNSKLHIAMFPWFAMGHMTPYVHLANELAKRGHKITFLLPN-KARLALHHLNRYPNLITF 70
SN LH+ MFP+FA GH++P+V LAN+L+ G K++F + A LN P T
Sbjct: 8 SNDALHVVMFPFFAFGHISPFVQLANKLSSYGVKVSFFTASGNASRVKSMLNSAP---TT 64
Query: 71 HII--TVPNVDGLPLGTETASEIPLSLNHLLVVAMDKTRNQVEQVMKSKKPHFVLFDVAY 128
HI+ T+P+V+GLP G E+ +E+ + LL VA+D + Q++ ++ KPHFVLFD A
Sbjct: 65 HIVPLTLPHVEGLPPGAESTAELTPASAELLKVALDLMQPQIKTLLSHLKPHFVLFDFAQ 124
Query: 129 -WVPEAARKLGIKTICYHVVSAMSMAMSFVPAKIIPKDRPLTVEDMSQPPPGYPSSSNSK 187
W+P+ A LGIKT+ Y VV A+S A PA+++ + ++EDM +PP G+P +S +
Sbjct: 125 EWLPKMANGLGIKTVYYSVVVALSTAFLTCPARVLEPKKYPSLEDMKKPPLGFPQTSVTS 184
Query: 188 LVLRRYEAEQQMFASIPFGEGGVTLYDRTTTGLRECDAIAIKTTTEVEGCFCDYIAIQYH 247
+ R +EA ++ F G TLYDR +GLR C AI KT +++EG + Y+ Q++
Sbjct: 185 V--RTFEARDFLYVFKSF-HNGPTLYDRIQSGLRGCSAILAKTCSQMEGPYIKYVEAQFN 241
Query: 248 KNVLLTGPVLSEEAEGEELEERWAKWLDGLGLGLGLGLGLGPVVYCAFGSQVILGKAQFQ 307
K V L GPV+ + G +LEE+WA WL+ G V+YC+FGS+ L Q +
Sbjct: 242 KPVFLIGPVVPDPPSG-KLEEKWATWLN--------KFEGGTVIYCSFGSETFLTDDQVK 292
Query: 308 EILLGLEISGFSIF 321
E+ LGLE +G F
Sbjct: 293 ELALGLEQTGLPFF 306
>MGT_STRLI (Q54387) Macrolide glycosyltransferase (EC 2.4.1.-)
Length = 418
Score = 42.0 bits (97), Expect = 0.004
Identities = 35/122 (28%), Positives = 55/122 (44%), Gaps = 9/122 (7%)
Query: 17 HIAMFPWFAMGHMTPYVHLANELAKRGHKITFLLPNKARLALHHLNRYPNLITFHIITVP 76
HIAMF GH+ P + + EL RGH++T+ +P + P L T+P
Sbjct: 24 HIAMFSIALHGHVNPSLEVIRELVARGHRVTYAIPRLLADKVAEAGAEPKLWN---STLP 80
Query: 77 NVDGLPLGTETASEIPLSLNHLLVVAMDKTRNQVEQVMKSKKPHFVLFDVAYWVPEAARK 136
D P S + ++ L A+ ++ Q+ Q + +P VL D+A + AR
Sbjct: 81 GPDADP--EAWGSTLLDNVEPFLADAI-QSLPQLAQAYEGDEPDLVLHDIASY---TARV 134
Query: 137 LG 138
LG
Sbjct: 135 LG 136
>OLED_STRAT (Q53685) Oleandomycin glycosyltransferase (EC 2.4.1.-)
Length = 430
Score = 40.4 bits (93), Expect = 0.010
Identities = 16/42 (38%), Positives = 26/42 (61%)
Query: 10 SSSNSKLHIAMFPWFAMGHMTPYVHLANELAKRGHKITFLLP 51
++ + HIAMF A GH+ P + + EL RGH++T+ +P
Sbjct: 2 TTQTTPAHIAMFSIAAHGHVNPSLEVIRELVARGHRVTYAIP 43
>HQGT_RAUSE (Q9AR73) Hydroquinone glucosyltransferase (EC 2.4.1.218)
(Arbutin synthase)
Length = 470
Score = 40.4 bits (93), Expect = 0.010
Identities = 64/311 (20%), Positives = 124/311 (39%), Gaps = 36/311 (11%)
Query: 17 HIAMFPWFAMGHMTPYVHLANELAKR-GHKITFLLPNKARL---ALHHLNRYPNLITFHI 72
HIAM P MGH+ P V A L R +TF++P L L+ P + + +
Sbjct: 6 HIAMVPTPGMGHLIPLVEFAKRLVLRHNFGVTFIIPTDGPLPKAQKSFLDALPAGVNYVL 65
Query: 73 ITVPNVDGLPLGTETASEIPLSLNHLLVVAMDKTRNQVEQVMKSKKPHFVLFDV-AYWVP 131
+ + D LP + I L++ ++ R+ V+ ++ + K ++ D+
Sbjct: 66 LPPVSFDDLPADVRIETRICLTITR----SLPFVRDAVKTLLATTKLAALVVDLFGTDAF 121
Query: 132 EAARKLGIKTICYHVVSAMSMAMSFVPAKIIPKDRPLTVE--DMSQP--PPGYPSSSNSK 187
+ A + + ++ +AM +++ F K+ D+ ++ E D+ +P PG
Sbjct: 122 DVAIEFKVSPYIFYPTTAMCLSLFFHLPKL---DQMVSCEYRDVPEPLQIPGCIPIHGKD 178
Query: 188 LVLRRYEAEQQMFASIPFGEGGVTLYDRTTTGLRECDAIAIKTTTEVEGCFCDYI--AIQ 245
+ + + + + R + I + T ++E + Q
Sbjct: 179 FLDPAQDRKNDAYKCLL----------HQAKRYRLAEGIMVNTFNDLEPGPLKALQEEDQ 228
Query: 246 YHKNVLLTGPVLSEEAEGEELEERWAKWLDGLGLGLGLGLGLGPVVYCAFGSQVILGKAQ 305
V GP++ ++ + + KWLD G V++ +FGS + Q
Sbjct: 229 GKPPVYPIGPLIRADSSSKVDDCECLKWLD--------DQPRGSVLFISFGSGGAVSHNQ 280
Query: 306 FQEILLGLEIS 316
F E+ LGLE+S
Sbjct: 281 FIELALGLEMS 291
>ZOX_PHAVU (P56725) Zeatin O-xylosyltransferase (EC 2.4.2.40)
(Zeatin O-beta-D-xylosyltransferase)
Length = 454
Score = 34.3 bits (77), Expect = 0.74
Identities = 65/322 (20%), Positives = 116/322 (35%), Gaps = 57/322 (17%)
Query: 11 SSNSKLHIAMFPWFAMGHMTPYVHLANELAKRGHKITFL--LPNKARLALHHLNRYPNLI 68
+ +K+ + + P+ GH+ P++ L++ +A + + ++ + + + L + N N I
Sbjct: 4 NDETKVVVLLLPFPVQGHLNPFLQLSHLIAAQNIAVHYVGTVTHIRQAKLRYHNATSN-I 62
Query: 69 TFHIITVPNVDGLPLGTETASEIPLSLNHLLVVAMDKTRNQVEQVMKSKKPHFVLFD--- 125
FH VP P E L + + + ++ Q + S+ VL +
Sbjct: 63 HFHAFEVPPYVSPPPNPEDDFPSHLIPSFEASAHLREPVGKLLQSLSSQAKRVVLINDSL 122
Query: 126 VAYWVPEAARKLGIKTICYHVVSAMSMAMSFVPAKIIPKDRPLTVEDMSQPP------PG 179
+A +AA ++ C+ V SA++ A F E M +PP P
Sbjct: 123 MASVAQDAANFSNVERYCFQVFSALNTAGDF-------------WEQMGKPPLADFHFPD 169
Query: 180 YPSSSNS-KLVLRRYEAEQQMFASIPFGEGGVTLYDRTTTGLRECDAIAIKTTTEVEGCF 238
PS + Q F F G + T+ +EG +
Sbjct: 170 IPSLQGCISAQFTDFLTAQNEFRK--FNNGDI-----------------YNTSRVIEGPY 210
Query: 239 CDYI-AIQYHKNVLLTG---PVLSEEAEGEELEERWAKWLDGLGLGLGLGLGLGPVVYCA 294
+ + K V G P+ E+ + +WLD V+Y +
Sbjct: 211 VELLERFNGGKEVWALGPFTPLAVEKKDSIGFSHPCMEWLD--------KQEPSSVIYVS 262
Query: 295 FGSQVILGKAQFQEILLGLEIS 316
FG+ L Q QE+ GLE S
Sbjct: 263 FGTTTALRDEQIQELATGLEQS 284
>UFO2_MANES (Q40285) Flavonol 3-O-glucosyltransferase 2 (EC
2.4.1.91) (UDP-glucose flavonoid 3-O-glucosyltransferase
2) (Fragment)
Length = 346
Score = 34.3 bits (77), Expect = 0.74
Identities = 27/99 (27%), Positives = 40/99 (40%), Gaps = 15/99 (15%)
Query: 221 RECDAIAIKTTTEVEGCFCDYIAIQ--YHKNVLLTGPVLSEEAEGEELEERWAKWLDGLG 278
R+ I + T E+E + + YH GP+L +++G +WLD
Sbjct: 89 RQAKGIIVNTFLELESRAIESFKVPPLYH-----VGPILDVKSDGRNTHPEIMQWLDDQP 143
Query: 279 LGLGLGLGLGPVVYCAFGSQVILGKAQFQEILLGLEISG 317
G VV+ FGS + Q +EI LE SG
Sbjct: 144 EG--------SVVFLCFGSMGSFSEDQLKEIAYALENSG 174
>HQGT_ARATH (Q9M156) Probable hydroquinone glucosyltransferase (EC
2.4.1.218) (Arbutin synthase)
Length = 480
Score = 33.9 bits (76), Expect = 0.96
Identities = 27/85 (31%), Positives = 43/85 (49%), Gaps = 10/85 (11%)
Query: 17 HIAMFPWFAMGHMTPYVHLANELAK-RGHKITFLL-----PNKARLALHHLNRYPNLITF 70
H+A+ P MGH+ P V A L G +TF++ P+KA+ + L+ P+ I+
Sbjct: 8 HVAIIPSPGMGHLIPLVEFAKRLVHLHGLTVTFVIAGEGPPSKAQRTV--LDSLPSSIS- 64
Query: 71 HIITVPNVDGLPLGTETASEIPLSL 95
+ +P VD L + T E +SL
Sbjct: 65 -SVFLPPVDLTDLSSSTRIESRISL 88
>ITB8_RABIT (P26013) Integrin beta-8 precursor
Length = 768
Score = 33.5 bits (75), Expect = 1.3
Identities = 22/56 (39%), Positives = 36/56 (64%), Gaps = 2/56 (3%)
Query: 94 SLNHLLVVAMDKTRNQVEQVMKSKKPHFVLFDVAYWVPEAARKLGIKTICYHVVSA 149
+LN+L+V A K ++V+ ++SK P V F+V P+ ARKLG++ C +V S+
Sbjct: 372 NLNNLVVEAYQKLISEVKVQVESKVPG-VYFNVTAICPDGARKLGMEG-CSNVTSS 425
>UFO1_MANES (Q40284) Flavonol 3-O-glucosyltransferase 1 (EC
2.4.1.91) (UDP-glucose flavonoid 3-O-glucosyltransferase
1)
Length = 449
Score = 31.6 bits (70), Expect = 4.8
Identities = 28/98 (28%), Positives = 39/98 (39%), Gaps = 15/98 (15%)
Query: 222 ECDAIAIKTTTEVEGCFCDYIAIQYHKN--VLLTGPVLSEEAEGEELEERWAKWLDGLGL 279
E + I T E+E AI+ K+ + GP+L + G + +WLD
Sbjct: 194 EAKGVIINTFFELESH-----AIESFKDPPIYPVGPILDVRSNGRNTNQEIMQWLDDQPP 248
Query: 280 GLGLGLGLGPVVYCAFGSQVILGKAQFQEILLGLEISG 317
VV+ FGS K Q +EI LE SG
Sbjct: 249 S--------SVVFLCFGSNGSFSKDQVKEIACALEDSG 278
>CRTX_ERWHE (Q01330) Zeaxanthin glucosyl transferase (EC 2.4.1.-)
Length = 413
Score = 31.6 bits (70), Expect = 4.8
Identities = 18/47 (38%), Positives = 23/47 (48%)
Query: 17 HIAMFPWFAMGHMTPYVHLANELAKRGHKITFLLPNKARLALHHLNR 63
H A+ H LA E+A+RGH++TFL N A LA R
Sbjct: 3 HFAIVAPPLYSHAVALHALALEMAQRGHRVTFLTGNVASLAEQETER 49
>RHO2_SCHPO (Q10133) Rho2 protein
Length = 200
Score = 30.8 bits (68), Expect = 8.2
Identities = 23/104 (22%), Positives = 43/104 (41%)
Query: 114 MKSKKPHFVLFDVAYWVPEAARKLGIKTICYHVVSAMSMAMSFVPAKIIPKDRPLTVEDM 173
M K H +L A P++ + K I ++ V K + P+ +E+M
Sbjct: 75 MSYAKAHIILVGFAIDSPDSLENVSTKWIEEINTLCPNVPFILVGMKADLRSDPVAIEEM 134
Query: 174 SQPPPGYPSSSNSKLVLRRYEAEQQMFASIPFGEGGVTLYDRTT 217
+ + S ++LV +R A + M S G+G +++ T
Sbjct: 135 RRRNQNFVKSQQAELVAQRIGARKYMECSSLTGDGVDDVFEAAT 178
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.334 0.148 0.485
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,497,771
Number of Sequences: 164201
Number of extensions: 2551150
Number of successful extensions: 8818
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 8806
Number of HSP's gapped (non-prelim): 14
length of query: 484
length of database: 59,974,054
effective HSP length: 114
effective length of query: 370
effective length of database: 41,255,140
effective search space: 15264401800
effective search space used: 15264401800
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.5 bits)
S2: 68 (30.8 bits)
Lotus: description of TM0282.12