BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0246c.4
(536 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YLE1_SCHPO (Q10451) Hypothetical protein C1093.01 in chromosome I 45 4e-04
APA1_SALTR (Q91488) Apolipoprotein A-I precursor (Apo-AI) 34 0.84
ANK1_MOUSE (Q02357) Ankyrin 1 (Erythrocyte ankyrin) 34 0.84
SYI_BUCAP (Q8K9Z2) Isoleucyl-tRNA synthetase (EC 6.1.1.5) (Isole... 34 1.1
RMLA_STRMU (P95778) Glucose-1-phosphate thymidylyltransferase (E... 33 1.9
AP12_ONCMY (O57524) Apolipoprotein A-I-2 precursor (APOA-I-2) 33 1.9
MET4_YEAST (P32389) Transcriptional activator of sulfur metaboli... 33 2.4
ADA_HUMAN (P00813) Adenosine deaminase (EC 3.5.4.4) (Adenosine a... 32 3.2
LPRC_HUMAN (P42704) 130 kDa leucine-rich protein (LRP 130) (GP13... 32 4.1
AROB_STAEP (Q8CP68) 3-dehydroquinate synthase (EC 4.2.3.4) 32 4.1
YVYE_BACSU (P32437) Hypothetical UPF0029 protein yvyE 31 7.1
STRM_STRGR (P29783) dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1... 31 7.1
HST6_CANAL (P53706) ATP-dependent permease HST6 (STE6 homolog) 31 7.1
EFP_STRAW (Q827R5) Elongation factor P (EF-P) 31 9.2
>YLE1_SCHPO (Q10451) Hypothetical protein C1093.01 in chromosome I
Length = 1261
Score = 45.4 bits (106), Expect = 4e-04
Identities = 33/124 (26%), Positives = 58/124 (46%), Gaps = 12/124 (9%)
Query: 182 GILPRKFCKAFHMHHEMTHKGFLPDFVSGFS-----------PSLVTYNALIHGLCLLGR 230
G +PR A H+ + T +G D + + PS+ YNA++ L R
Sbjct: 883 GYIPRASTFA-HLINNSTRRGDTDDATTALNIFEETKRHNVKPSVFLYNAVLSKLGRARR 941
Query: 231 VEEALGILRGMAAMGLPPDAVCYTTVISGLCKIGEPRKAYELKLEMDGKNFWWLDLDTYD 290
E + + M GL P +V Y TVI+ C+IG+ A +L EM+ + + + Y+
Sbjct: 942 TTECWKLFQEMKESGLLPTSVTYGTVINAACRIGDESLAEKLFAEMENQPNYQPRVAPYN 1001
Query: 291 SLME 294
++++
Sbjct: 1002 TMIQ 1005
Score = 32.7 bits (73), Expect = 2.4
Identities = 24/95 (25%), Positives = 42/95 (43%), Gaps = 14/95 (14%)
Query: 97 VVEEMNRKGLTPDENTNMSLF---------PLVSAYC-----VGERERVEKAVGILRVMA 142
++ R+G T D T +++F P V Y +G R + + + M
Sbjct: 894 LINNSTRRGDTDDATTALNIFEETKRHNVKPSVFLYNAVLSKLGRARRTTECWKLFQEMK 953
Query: 143 KRGLSPDAVSYTTVISGFCYLDEPELDKALEMKAE 177
+ GL P +V+Y TVI+ C + + L + L + E
Sbjct: 954 ESGLLPTSVTYGTVINAACRIGDESLAEKLFAEME 988
Score = 32.7 bits (73), Expect = 2.4
Identities = 24/100 (24%), Positives = 45/100 (45%), Gaps = 6/100 (6%)
Query: 59 AFGLWKAMTDKGVEPNLVSLNAVIQGLCEEERMEEAEEVVEEMNRKGLTPDENTNMSLFP 118
A +++ V+P++ NAV+ L R E ++ +EM GL P T+++
Sbjct: 910 ALNIFEETKRHNVKPSVFLYNAVLSKLGRARRTTECWKLFQEMKESGLLP---TSVTYGT 966
Query: 119 LVSAYC-VGERERVEKAVGILRVMAKRGLSPDAVSYTTVI 157
+++A C +G+ EK + + P Y T+I
Sbjct: 967 VINAACRIGDESLAEKL--FAEMENQPNYQPRVAPYNTMI 1004
>APA1_SALTR (Q91488) Apolipoprotein A-I precursor (Apo-AI)
Length = 262
Score = 34.3 bits (77), Expect = 0.84
Identities = 24/76 (31%), Positives = 35/76 (45%), Gaps = 6/76 (7%)
Query: 64 KAMTDKGVEPNLVSLNAVIQGLCEEERME------EAEEVVEEMNRKGLTPDENTNMSLF 117
K + DK ++ L +I+ + E+ R E + E VVEEM K T E T L
Sbjct: 125 KEVLDKHIDEYRKKLEPLIKEIVEQRRTELEAFRVKMEPVVEEMRAKVSTNVEETKAKLM 184
Query: 118 PLVSAYCVGERERVEK 133
P+V ER+E+
Sbjct: 185 PIVETVRAKLTERLEE 200
>ANK1_MOUSE (Q02357) Ankyrin 1 (Erythrocyte ankyrin)
Length = 1862
Score = 34.3 bits (77), Expect = 0.84
Identities = 62/243 (25%), Positives = 99/243 (40%), Gaps = 39/243 (16%)
Query: 47 IVARCKEKKVREAFGLWKAMTDKGVEPNLVSLNAV--IQGLCEEERMEEAEEVVEEMNRK 104
+ A C +V K + DKG +PN +LN + C++ + V+E + +
Sbjct: 340 VAAHCGHHRVA------KVLLDKGAKPNSRALNGFTPLHIACKKNHI----RVMELLLKT 389
Query: 105 GLTPDENTNMSLFPLVSAYCVGERERVEKAVGILRVMAKRGLSPDA--VSYTTVISGFCY 162
G + D T L PL A +G + I++ + +RG SP+ V T +
Sbjct: 390 GASIDAVTESGLTPLHVASFMGH-------LPIVKNLLQRGASPNVSNVKVETPLHMAAR 442
Query: 163 LDEPELDK-ALEMKAEMLHKGILPRKFCKAFHMHHEMTHKGFLPDFV-SGFSPSLVTY-- 218
E+ K L+ KA+ K + H + H G + + +G SP+L T
Sbjct: 443 AGHTEVAKYLLQNKAKANAKA---KDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAG 499
Query: 219 NALIHGLCLLGRVEEALGILRGMAAMGLPPDAVCYT----TVISGLCKIGEPRKAYELKL 274
+ +H G V+ AL +L A+ C T T + K G+ R A EL L
Sbjct: 500 HTPLHTAAREGHVDTALALLEKEASQ------ACMTKKGFTPLHVAAKYGKVRLA-ELLL 552
Query: 275 EMD 277
E D
Sbjct: 553 EHD 555
>SYI_BUCAP (Q8K9Z2) Isoleucyl-tRNA synthetase (EC 6.1.1.5)
(Isoleucine--tRNA ligase) (IleRS)
Length = 938
Score = 33.9 bits (76), Expect = 1.1
Identities = 34/129 (26%), Positives = 56/129 (43%), Gaps = 16/129 (12%)
Query: 379 ENCSNNEFKSVVGLVKDFSMRGLVGDAGRARDTMLHGNYKPEGSVYNLLIFDHCRSHSVN 438
+ C K + KDF G++GD A TM ++K E ++ L S +
Sbjct: 124 KKCRKYAEKQIKKQKKDFIRLGVIGDWENAHLTM---DFKNEANIIKTL------SKIIE 174
Query: 439 KAYDMYKEMVHYGFAPHMFSILAVIKALHYDRRYNEMMWVIKNTLKSCNLNDSELRKVLN 498
K Y +YKE + +S L+ + H+D+ + + IK+ N+ L++VLN
Sbjct: 175 KKY-LYKESKPIHWCLKCYSSLSDAEIEHFDKESDSIFVAIKSK------NNKILKEVLN 227
Query: 499 KINVRECDI 507
E DI
Sbjct: 228 LNISSEKDI 236
>RMLA_STRMU (P95778) Glucose-1-phosphate thymidylyltransferase (EC
2.7.7.24) (dTDP-glucose synthase) (dTDP-glucose
pyrophosphorylase)
Length = 289
Score = 33.1 bits (74), Expect = 1.9
Identities = 25/83 (30%), Positives = 43/83 (51%), Gaps = 14/83 (16%)
Query: 259 GLCKIGEPRKAY----ELKLEMDGKNFWWLDLDTYDSLMEDLSDEDIYSNMMNDYFAQGN 314
G +I + KAY +L +E+ + F WLD T++SL+E + M N A N
Sbjct: 193 GELEITDVNKAYLDRGDLSVEVMERGFAWLDTGTHESLLEAAQYIETVQRMQNLQVA--N 250
Query: 315 MKKV-YRLDYDMKHDGYLTSDVI 336
++++ YR+ GY+T+D +
Sbjct: 251 LEEIAYRM-------GYITADQV 266
>AP12_ONCMY (O57524) Apolipoprotein A-I-2 precursor (APOA-I-2)
Length = 262
Score = 33.1 bits (74), Expect = 1.9
Identities = 30/96 (31%), Positives = 42/96 (43%), Gaps = 14/96 (14%)
Query: 64 KAMTDKGVEPNLVSLNAVIQGLCEEERME------EAEEVVEEMNRKGLTPDENTNMSLF 117
K + DK ++ L +I+ + E+ R E + E VVEEM K E T L
Sbjct: 125 KEVLDKHIDEYRKRLEPLIKDIVEQRRTELEAFRVKIEPVVEEMRAKVSANVEETKAKLM 184
Query: 118 PLVSAYCVGERERVEKAVGILRVMAKRGLSPDAVSY 153
P+V ER+E+ LR +A SP A Y
Sbjct: 185 PIVETVRAKLTERLEE----LRTLA----SPYAEEY 212
>MET4_YEAST (P32389) Transcriptional activator of sulfur metabolism
MET4
Length = 634
Score = 32.7 bits (73), Expect = 2.4
Identities = 19/65 (29%), Positives = 28/65 (42%), Gaps = 3/65 (4%)
Query: 437 VNKAYDMYKEMVHYGFAPHMFSILAVIKALHYDRRYNEMMWVIKNTLKSCNLNDSELRKV 496
VN Y ++ GF +A I A H+ ++ E N K+ N NDS+ +
Sbjct: 327 VNVPNGAYNSLISAGFDNDQIDAIAAIMAYHHQKKIRENN---SNNNKNINTNDSQEAPI 383
Query: 497 LNKIN 501
L IN
Sbjct: 384 LKNIN 388
>ADA_HUMAN (P00813) Adenosine deaminase (EC 3.5.4.4) (Adenosine
aminohydrolase)
Length = 362
Score = 32.3 bits (72), Expect = 3.2
Identities = 26/85 (30%), Positives = 37/85 (42%), Gaps = 4/85 (4%)
Query: 287 DTYDSLMEDLSDEDIYSNMMNDYFAQGNMKKVYRLDYDM-KHDGYLTSDVIFRVYLNALN 345
DT +++ +D+ YS +D K DY M K D T + R+ +NA
Sbjct: 274 DTEHAVIRLKNDQANYSLNTDDPLI---FKSTLDTDYQMTKRDMGFTEEEFKRLNINAAK 330
Query: 346 KKARTIDAKRTLLDMIYKNLWTKPS 370
D KR LLD++YK PS
Sbjct: 331 SSFLPEDEKRELLDLLYKAYGMPPS 355
>LPRC_HUMAN (P42704) 130 kDa leucine-rich protein (LRP 130) (GP130)
(Leucine-rich PPR-motif containing protein)
Length = 1273
Score = 32.0 bits (71), Expect = 4.1
Identities = 39/183 (21%), Positives = 75/183 (40%), Gaps = 11/183 (6%)
Query: 18 FRNGMSVNIY-NFRIRGFAGAGNFESESALIVARCKEKKVREAFGLWKAMTDKGVEPNLV 76
F M V + I+GF N + S LI+ + + ++EA K + D+ P+ +
Sbjct: 962 FTQAMEVKAFAETHIKGFTL--NDAANSRLIITQVRRDYLKEAVTTLKTVLDQQQTPSRL 1019
Query: 77 SLNAVIQGLCEEERMEEAEEVVEEMNRKGLTPDENTNMSLFPLVSAYCVGERERVEKAVG 136
++ VIQ L + +E E V + +N GL + +F A + ++ A+
Sbjct: 1020 AVTRVIQALAMKGDVENIEVVQKMLN--GLEDSIGLSKMVFINNIALAQIKNNNIDAAIE 1077
Query: 137 ILRVMAKRGLSPDAVSYTTVISGFCYLDEPELDKALEMKAEMLHKGILPRKFCKAFHMHH 196
+ M L+ + G YL +++ LE E + I+ + F ++
Sbjct: 1078 NIENM----LTSENKVIEPQYFGLAYLFRKVIEEQLEPAVEKI--SIMAERLANQFAIYK 1131
Query: 197 EMT 199
+T
Sbjct: 1132 PVT 1134
>AROB_STAEP (Q8CP68) 3-dehydroquinate synthase (EC 4.2.3.4)
Length = 354
Score = 32.0 bits (71), Expect = 4.1
Identities = 28/105 (26%), Positives = 45/105 (42%), Gaps = 12/105 (11%)
Query: 414 HGNYKPEGSVYNLLIFDHCRSHSVN-KAYDMYKEMVHYGFAPHMFSILAVIKALHYDRRY 472
HG+ G +Y ++ +H + N + Y Y + + Y L++IK LH++ Y
Sbjct: 256 HGHAVMIGILYQFIVANHLFETNYNIQHYINYMKKLKYP--------LSIIKQLHFEDTY 307
Query: 473 NEMMWVIKNTLKSCNLNDSELRKVLNKINVRECDIDALLDVLAEI 517
+ M+ KN + L K L K V D D LL E+
Sbjct: 308 HFMLLDKKNDYNGIQM---VLLKNLGKPVVTHVDKDTLLSAFEEL 349
>YVYE_BACSU (P32437) Hypothetical UPF0029 protein yvyE
Length = 217
Score = 31.2 bits (69), Expect = 7.1
Identities = 30/124 (24%), Positives = 52/124 (41%), Gaps = 22/124 (17%)
Query: 92 EEAEEVVEEMNRKGLTPDENTNMSLFPLVSAYCVGERERVEKA-----------VGILRV 140
+EA+E ++++ ++ N SAY +GE + ++KA V +L V
Sbjct: 34 QEAQEFIQKIKKQHWNATHNC--------SAYVIGENDHIQKANDDGEPSGTAGVPMLEV 85
Query: 141 MAKRGLSPDAVSYTTVISGFCYLDEPELDKAL--EMKAEMLHKGILPRKFCKAFHMHHEM 198
+ KR L D + T G L L +A + + H G++ RK + H +
Sbjct: 86 LKKRRLK-DTCAVVTRYFGGIKLGAGGLIRAYGKSVSEGLNHIGVVERKLMRIMHTSADY 144
Query: 199 THKG 202
T G
Sbjct: 145 TWLG 148
>STRM_STRGR (P29783) dTDP-4-dehydrorhamnose 3,5-epimerase (EC
5.1.3.13) (dTDP-4-keto-6-deoxyglucose 3,5-epimerase)
(dTDP-L-rhamnose synthetase)
Length = 200
Score = 31.2 bits (69), Expect = 7.1
Identities = 28/110 (25%), Positives = 47/110 (42%), Gaps = 9/110 (8%)
Query: 224 GLCLLGRVEEALGILRGMAAMGLPPDAVCYTTVISGL-------CKIGEPR-KAYELKLE 275
G+ + R G+LRG+ LPP Y T +SG + G P +A+E
Sbjct: 42 GVAQVNRSVSRRGVLRGVHFAQLPPSQAKYVTCLSGAVLDVVVDIRTGSPTYRAWEAVRL 101
Query: 276 MDGKNFWWLDLDTYDSLMEDLSDEDIYSNMMNDYFAQGNMKKVYRLDYDM 325
D +++ S M L+D+ + + + +A G V+ LD D+
Sbjct: 102 DDPHRSLYVEAGLGHSFMA-LTDDAVVVYLTSQGYAAGREHGVHPLDPDL 150
>HST6_CANAL (P53706) ATP-dependent permease HST6 (STE6 homolog)
Length = 1323
Score = 31.2 bits (69), Expect = 7.1
Identities = 29/132 (21%), Positives = 61/132 (45%), Gaps = 18/132 (13%)
Query: 385 EFKSVVGLVKDFSMRGLVGDAGRARDTMLHGNYKPEGSVYNLLIFDHCRSHSVNKAYDMY 444
+ KS+ VK F+ G+VG +G + T+L ++ +Y++ I S++ K +D
Sbjct: 1053 QLKSISLDVKKFTTIGIVGQSGSGKSTILKILFR----LYDIKISPD--SNTTKKYHDQT 1106
Query: 445 KE-------MVHYGFAPHMFSILAVIKALHYDRRYNEMMWVIKNT-----LKSCNLNDSE 492
+ +++ G +I+ Y+ + + I NT S +++DSE
Sbjct: 1107 VKIFNQNLYLINSGLLCQTIAIVPQFPKFFSGTIYDNLTYGINNTNSAGSNSSSSVSDSE 1166
Query: 493 LRKVLNKINVRE 504
+ K+L +N+ +
Sbjct: 1167 IIKILKLVNLHQ 1178
>EFP_STRAW (Q827R5) Elongation factor P (EF-P)
Length = 188
Score = 30.8 bits (68), Expect = 9.2
Identities = 14/35 (40%), Positives = 21/35 (60%)
Query: 276 MDGKNFWWLDLDTYDSLMEDLSDEDIYSNMMNDYF 310
MDG+ F ++D+DTYD LM D +N + + F
Sbjct: 76 MDGEYFVFMDMDTYDQLMVDRKSVGDAANFLIEGF 110
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.321 0.137 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,750,662
Number of Sequences: 164201
Number of extensions: 2792363
Number of successful extensions: 7269
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 7250
Number of HSP's gapped (non-prelim): 30
length of query: 536
length of database: 59,974,054
effective HSP length: 115
effective length of query: 421
effective length of database: 41,090,939
effective search space: 17299285319
effective search space used: 17299285319
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 68 (30.8 bits)
Lotus: description of TM0246c.4