BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0083.3
         (287 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

M310_ARATH (P93295) Hypothetical mitochondrial protein AtMg00310...    45  3e-04
PHFD_MOUSE (Q8K2W6) PHD finger protein 13                              31  3.1
MIAM_SARMU (Q08668) Major microneme antigen precursor                  30  5.2
ACO2_SYNY3 (P74582) Aconitate hydratase 2 (EC 4.2.1.3) (Citrate ...    30  5.2
NIR_RHIHE (Q60214) Copper-containing nitrite reductase precursor...    30  8.9
MANB_BOVIN (Q29444) Beta-mannosidase precursor (EC 3.2.1.25) (Ma...    30  8.9
GLK1_STRCO (Q9ADA7) Glycerol kinase 1 (EC 2.7.1.30) (ATP:glycero...    30  8.9

>M310_ARATH (P93295) Hypothetical mitochondrial protein AtMg00310
           (ORF154)
          Length = 154

 Score = 44.7 bits (104), Expect = 3e-04
 Identities = 33/122 (27%), Positives = 54/122 (44%), Gaps = 14/122 (11%)

Query: 53  DYWRRSDEGRSHLGTVVRDCVNWKQIVLPK-NQGGLGVRSTRESNVSLLGKLAWK----- 106
           ++W  S E +  +  V      W+++   K + GGLG R     N +LL K +++     
Sbjct: 27  EFWWSSCENKRKISWVA-----WQKLCKSKEDDGGLGFRDLGWFNQALLAKQSFRIIHQP 81

Query: 107 --LGSRIFITKYLKGSSIFSYELTGSSSYVWKGLVKAFSVLRDGLSFAIHDGC-TSFWYS 163
             L SR+  ++Y   SS+    +    SY W+ ++    +L  GL   I DG  T  W  
Sbjct: 82  HTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLRTIGDGIHTKVWLD 141

Query: 164 DW 165
            W
Sbjct: 142 RW 143


>PHFD_MOUSE (Q8K2W6) PHD finger protein 13
          Length = 296

 Score = 31.2 bits (69), Expect = 3.1
 Identities = 19/56 (33%), Positives = 30/56 (52%), Gaps = 3/56 (5%)

Query: 188 SDVWVEGSWNLSMLYTELPDPY--KPTITSMQGPSHETVDDCWVWNSN-PAGYSCS 240
           SD+ V+  +  S+L  E  DPY   P+  +MQ     + D C  W+S+ P+  SC+
Sbjct: 123 SDIPVKEGFRESLLKLEAADPYVETPSSPTMQDIPQASADPCSGWDSDTPSSGSCA 178


>MIAM_SARMU (Q08668) Major microneme antigen precursor
          Length = 241

 Score = 30.4 bits (67), Expect = 5.2
 Identities = 16/72 (22%), Positives = 31/72 (42%), Gaps = 5/72 (6%)

Query: 209 YKPTITSMQGPSHETVDDCWVWNSNPAGYSCSDAYQWLRDWTVFRVLSVLGSMSSIISLF 268
           Y   +T  +   H   D+CW+ + NP G      +Q+      F   +  G+   ++  F
Sbjct: 171 YTGDMTGPRSCEHTCTDNCWMHSGNPLG-----TFQYSGHAPAFCWAACKGTAGCVMYTF 225

Query: 269 EEHMCKKSKENA 280
           +  +CK   +N+
Sbjct: 226 QGGVCKLYSKNS 237


>ACO2_SYNY3 (P74582) Aconitate hydratase 2 (EC 4.2.1.3) (Citrate
           hydro-lyase 2) (Aconitase 2)
          Length = 868

 Score = 30.4 bits (67), Expect = 5.2
 Identities = 17/52 (32%), Positives = 28/52 (53%), Gaps = 7/52 (13%)

Query: 178 VHISDT---ALTISDVWVEGSWNLSMLYTELPDPYKPTITSMQGPSHETVDD 226
           +H+SDT   A  + D W EG+W ++    E+P+  + T+T  + P     DD
Sbjct: 135 LHLSDTNGYAKQVIDAWAEGAWFINK--PEVPE--RITVTVFKVPGETNTDD 182


>NIR_RHIHE (Q60214) Copper-containing nitrite reductase precursor
           (EC 1.7.2.1) (Cu-NIR)
          Length = 377

 Score = 29.6 bits (65), Expect = 8.9
 Identities = 22/85 (25%), Positives = 35/85 (40%), Gaps = 15/85 (17%)

Query: 90  RSTRESNVSLLGKLAWKLGSRI----------FITKYLKGSSIFSYELTGSSSYVWKGLV 139
           R TR   +   G+  W+ G  +          FI    +G++ +++E  G  +YV   L+
Sbjct: 287 RDTRPHLIGGHGEYVWRTGKFVNVPDRDQETWFIPGPTRGAAYYTFEQPGIYAYVNHNLI 346

Query: 140 KAFSVLRDGLSFAIHDGCTSFWYSD 164
           +AF      L  A H   T  W  D
Sbjct: 347 EAFE-----LGAAAHFKVTGDWNDD 366


>MANB_BOVIN (Q29444) Beta-mannosidase precursor (EC 3.2.1.25)
           (Mannanase) (Mannase)
          Length = 879

 Score = 29.6 bits (65), Expect = 8.9
 Identities = 30/118 (25%), Positives = 46/118 (38%), Gaps = 25/118 (21%)

Query: 176 PFVHISDT--ALTISDVWVEGSWNLSMLYTELPDPYKPTITSMQGPSHETVDDCWVWNSN 233
           PF+  S T  A TI++ W+             P+PY      +    ++ V DCW W + 
Sbjct: 501 PFITSSPTNGAKTIAEGWLS------------PNPYDLNYGDVH--FYDYVSDCWNWRTF 546

Query: 234 PAGYSCSD-AYQWLRDWTVFRVLSVLG-----SMSSIISLFEEHMCKKSKENATGMEL 285
           P     S+  YQ    W  F  L  +      S  S  +L  +H+   + E    +EL
Sbjct: 547 PKARFVSEYGYQ---SWPSFSTLEKVSSEEDWSYRSSFALHRQHLINGNNEMLHQIEL 601


>GLK1_STRCO (Q9ADA7) Glycerol kinase 1 (EC 2.7.1.30) (ATP:glycerol
           3-phosphotransferase 1) (Glycerokinase 1) (GK 1)
          Length = 512

 Score = 29.6 bits (65), Expect = 8.9
 Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 5/60 (8%)

Query: 12  VMGPVLTPVLSEALCLRWAIGMARDLGFRHVWFDTDCLVLFDYWRRSDEGRSHLGTVVRD 71
           V  PV+ P+++E  CL  A      +GF   W  TD L     WRR+ E    +   +RD
Sbjct: 437 VDAPVVRPMVAETTCLGAAYAAGLAVGF---WNSTDDLRA--NWRRAAEWTPRMDADIRD 491


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.321    0.136    0.446 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,017,118
Number of Sequences: 164201
Number of extensions: 1539268
Number of successful extensions: 2819
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 2819
Number of HSP's gapped (non-prelim): 7
length of query: 287
length of database: 59,974,054
effective HSP length: 109
effective length of query: 178
effective length of database: 42,076,145
effective search space: 7489553810
effective search space used: 7489553810
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 65 (29.6 bits)