BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0022.14
         (334 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

YL09_YEAST (Q06078) Hypothetical 104.8 kDa Trp-Asp repeats conta...    36  0.12
NHR1_HUMAN (O14745) Ezrin-radixin-moesin binding phosphoprotein ...    32  2.2
PLPB_PASHA (Q08869) Outer membrane lipoprotein 2 precursor (PLP2)      31  3.8
YHH5_YEAST (P38760) Hypothetical 75.9 kDa protein in SPO13-ARG4 ...    31  5.0
NHR1_RAT (Q9JJ19) Ezrin-radixin-moesin binding phosphoprotein 50...    31  5.0

>YL09_YEAST (Q06078) Hypothetical 104.8 kDa Trp-Asp repeats
           containing protein in RPL31B-VIP1 intergenic region
          Length = 939

 Score = 36.2 bits (82), Expect = 0.12
 Identities = 29/102 (28%), Positives = 41/102 (39%), Gaps = 12/102 (11%)

Query: 150 HAITSTSFFDFFYYPLDAFSSKQLMTPDQSHSFGGVTLVTKKEIKRKNSYDSTPTLILSR 209
           H    TS  D  +Y LD  S   ++      S+GGVT  T          +  P ++ S 
Sbjct: 263 HLSVGTSSGDLIFYDLDRRSRIHVLKNIHRESYGGVTQAT--------FLNGQPIIVTSG 314

Query: 210 GAQSVSPQHSFRQVSQTVDTVLHNPPRHLESSSPRRVNHREP 251
           G  S+        +SQ    V+  PPR+L S    R  H +P
Sbjct: 315 GDNSLKEYVFDPSLSQGSGDVVVQPPRYLRS----RGGHSQP 352


>NHR1_HUMAN (O14745) Ezrin-radixin-moesin binding phosphoprotein 50
           (EBP50) (Na(+)/H(+) exchange regulatory cofactor NHE-RF)
           (NHERF-1) (Regulatory cofactor of Na(+)/H(+) exchanger)
           (Sodium-hydrogen exchanger regulatory factor 1) (Solute
           carrier family 9 i
          Length = 358

 Score = 32.0 bits (71), Expect = 2.2
 Identities = 26/116 (22%), Positives = 54/116 (46%), Gaps = 18/116 (15%)

Query: 166 DAFSSKQLMTPDQSHSFGGVTL-VTKKEIKRKNSYDS-------TPTLILSRGAQSVSPQ 217
           D F  K  + P Q H  G + +  T  EI+++NS ++       +P   L R A S + +
Sbjct: 236 DEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSE 295

Query: 218 HSFRQVSQTVDTVLHNPPRHLESSSPRRVNHREPVRHGHLTMGVVRQSTHRRREGQ 273
               Q S         PP+  +S++P   +  +P+   ++++ + ++  H++R  +
Sbjct: 296 ELNSQDS---------PPKQ-DSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSK 341


>PLPB_PASHA (Q08869) Outer membrane lipoprotein 2 precursor (PLP2)
          Length = 276

 Score = 31.2 bits (69), Expect = 3.8
 Identities = 14/46 (30%), Positives = 26/46 (56%)

Query: 1   MSPMKSSEVDKPEEKPLTGKLVRVEDVQDEPLAVDYTQSFTTDKVF 46
           +SP K   + + ++ P    +V  ED +D+P    + +SF T++VF
Sbjct: 216 LSPDKDGIIVESKDSPYVNLVVSREDNKDDPRLQTFVKSFQTEEVF 261


>YHH5_YEAST (P38760) Hypothetical 75.9 kDa protein in SPO13-ARG4
           intergenic region
          Length = 659

 Score = 30.8 bits (68), Expect = 5.0
 Identities = 19/68 (27%), Positives = 33/68 (47%)

Query: 169 SSKQLMTPDQSHSFGGVTLVTKKEIKRKNSYDSTPTLILSRGAQSVSPQHSFRQVSQTVD 228
           S  +  T + S    G+T   ++ +   +SY ++ T + S  A  + P  +F  ++ TVD
Sbjct: 507 SMMRFRTQELSAHQKGLTSQFQQVVSPFSSYSNSYTNMNSLVATPMKPHPAFNLITNTVD 566

Query: 229 TVLHNPPR 236
             LH P R
Sbjct: 567 EKLHQPKR 574


>NHR1_RAT (Q9JJ19) Ezrin-radixin-moesin binding phosphoprotein 50
           (EBP50) (Na(+)/H(+) exchange regulatory cofactor NHE-RF)
           (NHERF-1) (Regulatory cofactor of Na(+)/H(+) exchanger)
           (Sodium-hydrogen exchanger regulatory factor 1) (Solute
           carrier family 9 iso
          Length = 356

 Score = 30.8 bits (68), Expect = 5.0
 Identities = 26/116 (22%), Positives = 51/116 (43%), Gaps = 17/116 (14%)

Query: 166 DAFSSKQLMTPDQSHSFGGVTL-VTKKEIKRKNSYDS-------TPTLILSRGAQSVSPQ 217
           D F  K  +TP Q H  G +    +  EI+++NS ++       +P   L+R A S + +
Sbjct: 233 DEFFKKCRVTPSQEHLDGPLPEPFSNGEIQKENSREALVEPASESPRPALARSASSDTSE 292

Query: 218 HSFRQVSQTVDTVLHNPPRHLESSSPRRVNHREPVRHGHLTMGVVRQSTHRRREGQ 273
               Q S         P RH  +      +  +P+   ++++ V ++  H++R  +
Sbjct: 293 ELNAQDS---------PKRHDSTEPSSTSSSSDPILDFNISLAVAKERAHQKRSSK 339


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.319    0.133    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,246,725
Number of Sequences: 164201
Number of extensions: 1571973
Number of successful extensions: 3357
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 3354
Number of HSP's gapped (non-prelim): 5
length of query: 334
length of database: 59,974,054
effective HSP length: 111
effective length of query: 223
effective length of database: 41,747,743
effective search space: 9309746689
effective search space used: 9309746689
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 66 (30.0 bits)