BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0008.5
(504 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PGL2_CRYJA (P43212) Polygalacturonase precursor (EC 3.2.1.15) (P... 244 3e-64
PGL2_CHAOB (Q7M1E7) Polygalacturonase precursor (PG) (EC 3.2.1.1... 243 1e-63
PGLR_ACTCH (P35336) Polygalacturonase precursor (EC 3.2.1.15) (P... 227 6e-59
PGL2_JUNAS (Q9FY19) Polygalacturonase precursor (PG) (EC 3.2.1.1... 225 2e-58
PGLR_PERAE (Q02096) Polygalacturonase precursor (EC 3.2.1.15) (P... 214 3e-55
PGLR_LYCES (P05117) Polygalacturonase 2A precursor (EC 3.2.1.15)... 207 4e-53
PGLR_MALDO (P48978) Polygalacturonase precursor (EC 3.2.1.15) (P... 206 9e-53
PGLR_PRUPE (P48979) Polygalacturonase precursor (EC 3.2.1.15) (P... 191 4e-48
PGLT_MAIZE (P35339) Exopolygalacturonase precursor (EC 3.2.1.67)... 173 8e-43
PGLR_MAIZE (P26216) Exopolygalacturonase precursor (EC 3.2.1.67)... 167 6e-41
PGL2_PLAAC (Q6H9K0) Exopolygalacturonase precursor (EC 3.2.1.67)... 166 1e-40
PGL2_ARATH (P49063) Exopolygalacturonase clone GBGA483 precursor... 166 1e-40
PGLS_MAIZE (P35338) Exopolygalacturonase precursor (EC 3.2.1.67)... 165 2e-40
PGL1_ARATH (P49062) Exopolygalacturonase clone GBGE184 precursor... 164 4e-40
PGLR_GOSHI (Q39786) Polygalacturonase precursor (EC 3.2.1.15) (P... 163 8e-40
PGLR_GOSBA (Q39766) Polygalacturonase precursor (EC 3.2.1.15) (P... 162 2e-39
PGLR_MEDSA (Q40312) Polygalacturonase precursor (EC 3.2.1.15) (P... 157 6e-38
PGLR_OENOR (P24548) Exopolygalacturonase (EC 3.2.1.67) (ExoPG) (... 145 2e-34
PGLR_TOBAC (Q05967) Polygalacturonase precursor (EC 3.2.1.15) (P... 134 5e-31
PGLR_BRANA (P35337) Polygalacturonase precursor (EC 3.2.1.15) (P... 131 4e-30
>PGL2_CRYJA (P43212) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase) (Major pollen allergen Cry j 2) (Cry j II)
Length = 514
Score = 244 bits (624), Expect = 3e-64
Identities = 140/369 (37%), Positives = 202/369 (53%), Gaps = 50/369 (13%)
Query: 113 MFNVLDFGAKGDGRTDDTKAFQATCAMACKVEASTMVVPAAYVFYVGPISFSGPYCKPNI 172
+FNV +GA GDG+ D T+AF ACK ++ ++VP F V + F+GP C+P+
Sbjct: 58 IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGP-CQPHF 116
Query: 173 VFQLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFPYDDPIDD 232
F++DG I A N +W + WL+F+KL G T+ GKGVIDG+G WW
Sbjct: 117 TFKVDGIIAAYQNPASWKNNRI-WLQFAKLTGFTLMGKGVIDGQGKQWWA---------G 166
Query: 233 EEKLIVPLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFSPTVTGITIQNSPQCHLKFD 292
+ K + + N+ R PTA++F S + G+ + NSP+ HL F
Sbjct: 167 QCKWV--------NGREICNDRDR------PTAIKFDFSTGLIIQGLKLMNSPEFHLVFG 212
Query: 293 NCNGVLVHDVSISSPGDSPNTDGI--------HLQ---------------NSKDVLIHSS 329
NC GV + +SI++P DSPNTDGI HLQ S +++I
Sbjct: 213 NCEGVKIIGISITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDL 272
Query: 330 KMACGHGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQGGSGSVQGVLFSNIQ 389
GHGISIGSLG++N+RA VS + + +T NG+RIKTWQGGSG +++ N++
Sbjct: 273 ICGPGHGISIGSLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVE 332
Query: 390 VSEVQLPIVIDQFYCDKRS-CTNHTSAVALAGINYEGIKGT-YTVKPVHFACSDSLPCVD 447
+ + PI+I+QFYC S C N SAV + + Y+ I+GT T + CSDS+PC D
Sbjct: 333 MINSENPILINQFYCTSASACQNQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDSMPCKD 392
Query: 448 VSLTSVELK 456
+ L+ + LK
Sbjct: 393 IKLSDISLK 401
>PGL2_CHAOB (Q7M1E7) Polygalacturonase precursor (PG) (EC 3.2.1.15)
(Pectinase) (Major pollen allergen Cha o 2)
Length = 514
Score = 243 bits (619), Expect = 1e-63
Identities = 140/372 (37%), Positives = 203/372 (53%), Gaps = 50/372 (13%)
Query: 110 SSTMFNVLDFGAKGDGRTDDTKAFQATCAMACKVEASTMVVPAAYVFYVGPISFSGPYCK 169
++T+FNV +GA GDG+ D T+AF T ACK ++ ++VPA F+V + F GP C+
Sbjct: 55 AATVFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGP-CQ 113
Query: 170 PNIVFQLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFPYDDP 229
P++ F++DGTI+A + W + WL+F++L + G GVIDG+G WW
Sbjct: 114 PHLSFKVDGTIVAQPDPARWKNSKI-WLQFAQLTDFNLMGTGVIDGQGQQWWAG------ 166
Query: 230 IDDEEKLIVPLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFSPTVTGITIQNSPQCHL 289
+ K++ V N+ R PTA++ S S TV +T+ NSP+ HL
Sbjct: 167 ---QCKVV--------NGRTVCNDRNR------PTAIKIDYSKSVTVKELTLMNSPEFHL 209
Query: 290 KFDNCNGVLVHDVSISSPGDSPNTDGIHLQNSK-----------------------DVLI 326
F C GV + + I +P DSPNTDGI + SK ++ I
Sbjct: 210 VFGECEGVKIQGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITI 269
Query: 327 HSSKMACGHGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQGGSGSVQGVLFS 386
GHGISIGSLG+DN+RA VS++ + +T NG+RIKTWQGGSG + +
Sbjct: 270 KDLICGPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYE 329
Query: 387 NIQVSEVQLPIVIDQFYCDKRS-CTNHTSAVALAGINYEGIKGT-YTVKPVHFACSDSLP 444
N+++ + PI+I+QFYC S C N SAV + G+ Y+ I GT T + CSDS+P
Sbjct: 330 NVEMINSENPILINQFYCTSASACQNQRSAVQIQGVTYKNIHGTSATAAAIQLMCSDSVP 389
Query: 445 CVDVSLTSVELK 456
C + L++V LK
Sbjct: 390 CTGIQLSNVSLK 401
>PGLR_ACTCH (P35336) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 467
Score = 227 bits (578), Expect = 6e-59
Identities = 135/372 (36%), Positives = 196/372 (52%), Gaps = 53/372 (14%)
Query: 110 SSTMFNVLDFGAKGDGRTDDTKAFQATCAMACKVEASTMVVPAAYVFYVGPISFSGPYCK 169
+S NV DFGAKGDGR DDTKAF+ AC +S +++ + V PISFSGP CK
Sbjct: 86 ASKTVNVDDFGAKGDGR-DDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGP-CK 143
Query: 170 PNIVFQLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFPYDDP 229
+ Q+ GTI A + + + WL F + + ++G G I+G G +WWQ+
Sbjct: 144 SGLTMQIYGTIEASDDRSDYRKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQN------ 197
Query: 230 IDDEEKLIVPLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFSPTVTGITIQNSPQCHL 289
+ +LP ++ PTAL FY S V + I+N+ Q H+
Sbjct: 198 -----------SCKTNKALPCKDA---------PTALTFYKSKHVIVKNLKIENAQQIHV 237
Query: 290 KFDNCNGVLVHDVSISSPGDSPNTDGIHLQNSKDVLIHS--------------------- 328
FDNC V ++ +++P +SPNTDGIH+ ++++ I S
Sbjct: 238 SFDNCVNVQASNLMVTAPENSPNTDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRV 297
Query: 329 SKMACG--HGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQGGSGSVQGVLFS 386
+ + CG HGISIGSLG N+ A VS++ + + T NGVRIKTWQGGSGS + F
Sbjct: 298 NDITCGPGHGISIGSLGYGNSEAHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQ 357
Query: 387 NIQVSEVQLPIVIDQFYCDK-RSCTNHTSAVALAGINYEGIKGTYTVK-PVHFACSDSLP 444
N+++ V+ PI+IDQ YCD+ + C +SAV + + Y+ IKGT + F CS P
Sbjct: 358 NVEMHNVENPIIIDQNYCDQDKPCQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSKRFP 417
Query: 445 CVDVSLTSVELK 456
C + L V+L+
Sbjct: 418 CQGIVLEDVDLE 429
>PGL2_JUNAS (Q9FY19) Polygalacturonase precursor (PG) (EC 3.2.1.15)
(Pectinase) (Major pollen allergen Jun a 2)
Length = 507
Score = 225 bits (573), Expect = 2e-58
Identities = 135/369 (36%), Positives = 195/369 (52%), Gaps = 50/369 (13%)
Query: 113 MFNVLDFGAKGDGRTDDTKAFQATCAMACKVEASTMVVPAAYVFYVGPISFSGPYCKPNI 172
+FNV +GA GDG+ D T AF+ T AC ++ +VPA F V + F GP C+P+
Sbjct: 59 VFNVEHYGAVGDGKHDSTDAFEKTWNAACNKLSAVFLVPANKKFVVNNLVFYGP-CQPHF 117
Query: 173 VFQLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFPYDDPIDD 232
F++DGTI A + W + W+ F++L + G GVIDG+G+ WW D
Sbjct: 118 SFKVDGTIAAYPDPAKWKNSKI-WMHFARLTDFNLMGTGVIDGQGNRWWSD--------- 167
Query: 233 EEKLIVPLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFSPTVTGITIQNSPQCHLKFD 292
+ K I V N+ GR PTA++ S S TV +T+ NSP+ HL F
Sbjct: 168 QCKTI--------NGRTVCNDKGR------PTAIKIDFSKSVTVKELTLTNSPEFHLVFG 213
Query: 293 NCNGVLVHDVSISSPGDSPNTDGIHLQNSK-----------------------DVLIHSS 329
C+GV + + I +P DSPNTDGI + SK ++ I
Sbjct: 214 ECDGVKIQGIKIKAPRDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDL 273
Query: 330 KMACGHGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQGGSGSVQGVLFSNIQ 389
GHG+SIGSLGK N+R+ VS + + +T NG+RIKTWQGGSG + + N++
Sbjct: 274 TCGPGHGMSIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVE 333
Query: 390 VSEVQLPIVIDQFYC-DKRSCTNHTSAVALAGINYEGIKGT-YTVKPVHFACSDSLPCVD 447
+ + PI+I+QFYC +C N SAV + + ++ I GT T + CSDS+PC +
Sbjct: 334 MINAENPILINQFYCTSAAACKNQRSAVKIQDVTFKNIHGTSATTAAIQLMCSDSVPCSN 393
Query: 448 VSLTSVELK 456
+ L++V LK
Sbjct: 394 IKLSNVFLK 402
>PGLR_PERAE (Q02096) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 462
Score = 214 bits (546), Expect = 3e-55
Identities = 134/387 (34%), Positives = 201/387 (51%), Gaps = 63/387 (16%)
Query: 95 VDPPSTPPSNDYNGGSSTMFNVLDFGAKGDGRTDDTKAFQATCAMACKVEASTMVVPAAY 154
V P T P D +V DFGA+GDG TDDTKAF+ AC S ++VP
Sbjct: 74 VGGPETSPDTD--------ISVDDFGARGDG-TDDTKAFEKAWKDACS-SGSVLIVPENK 123
Query: 155 VFYVGPISFSGPYCKPNIVFQLDGTIIAPTNSNAW-GGGLLQWLEFSKLVGITIQGKGVI 213
+ + I+FSGP CK ++ ++ GTI A ++ + W G +W+EF + +T++G G
Sbjct: 124 NYLLKQITFSGP-CKSDLRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTS 182
Query: 214 DGRGSVWWQDFPYDDPIDDEEKLIVPLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFS 273
+G G WW + +K SLP ++ PTAL F +
Sbjct: 183 NGNGETWWDS-----------------SCKRKKSLPCKSA---------PTALTFRSCKN 216
Query: 274 PTVTGITIQNSPQCHLKFDNCNGVLVHDVSISSPGDSPNTDGIHLQNSKDV--------- 324
V+ ++I++S + HL FD C V+ ++ +++P SPNTDGIH+ ++ +
Sbjct: 217 LIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEHSPNTDGIHITGTQRIHVMNSVIGT 276
Query: 325 ------------LIHSSKMACG--HGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRI 370
++ ++ + CG HGISIGSLG N+ A VS + + N+ +T NG+RI
Sbjct: 277 GDDCISIESGSKMVIATNITCGPGHGISIGSLGDRNSEAHVSGVLVDGGNLFDTTNGLRI 336
Query: 371 KTWQGGSGSVQGVLFSNIQVSEVQLPIVIDQFYCD-KRSCTNHTSAVALAGINYEGIKGT 429
KTWQGGSGS + + F NI + V PI+IDQ+YCD K C SAV ++ + Y I+GT
Sbjct: 337 KTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKDPCPEQESAVKVSNVAYMNIRGT 396
Query: 430 YTVK-PVHFACSDSLPCVDVSLTSVEL 455
+ V F CS S PC + ++ L
Sbjct: 397 SASEVAVKFDCSKSSPCQGYIVGNINL 423
>PGLR_LYCES (P05117) Polygalacturonase 2A precursor (EC 3.2.1.15)
(PG-2A) (Pectinase)
Length = 457
Score = 207 bits (528), Expect = 4e-53
Identities = 131/405 (32%), Positives = 200/405 (49%), Gaps = 56/405 (13%)
Query: 113 MFNVLDFGAKGDGRTDDTKAFQATCAMACKVEASTM-VVPAAYVFYVGPISFSGPYCKPN 171
+ NVL FGAKGDG+T D AF+ AC VVP + + I+FSGP C+ +
Sbjct: 75 VINVLSFGAKGDGKTYDNIAFEQAWNEACSSRTPVQFVVPKNKNYLLKQITFSGP-CRSS 133
Query: 172 IVFQLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFPYDDPID 231
I ++ G++ A + + + L W+ F + + + G G I+G G VWW
Sbjct: 134 ISVKIFGSLEASSKISDYKDRRL-WIAFDSVQNLVVGGGGTINGNGQVWW---------- 182
Query: 232 DEEKLIVPLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFSPTVTGITIQNSPQCHLKF 291
P + SLP ++ PTAL F+ + V + +N+ Q H+KF
Sbjct: 183 -------PSSCKINKSLPCRDA---------PTALTFWNCKNLKVNNLKSKNAQQIHIKF 226
Query: 292 DNCNGVLVHDVSISSPGDSPNTDGIHLQNSKDVLI---------------------HSSK 330
++C V+ ++ I++ SPNTDG+H+ N++ + I ++
Sbjct: 227 ESCTNVVASNLMINASAKSPNTDGVHVSNTQYIQISDTIIGTGDDCISIVSGSQNVQATN 286
Query: 331 MACG--HGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQGGSGSVQGVLFSNI 388
+ CG HGISIGSLG N+ A VSN+T+ + + NGVRIKTWQGGSG + F N+
Sbjct: 287 ITCGPGHGISIGSLGSGNSEAYVSNVTVNEAKIIGAENGVRIKTWQGGSGQASNIKFLNV 346
Query: 389 QVSEVQLPIVIDQFYCDK-RSCTNHTSAVALAGINYEGIKGTYTVK-PVHFACSDSLPCV 446
++ +V+ PI+IDQ YCD+ C SAV + + YE IKGT K + F CS + PC
Sbjct: 347 EMQDVKYPIIIDQNYCDRVEPCIQQFSAVQVKNVVYENIKGTSATKVAIKFDCSTNFPCE 406
Query: 447 DVSLTSVELKPVQEQYHLYEPFCWQTYGQLMTPTTPPIDCIQIGK 491
+ + ++ L V E E C + TP ++I +
Sbjct: 407 GIIMENINL--VGESGKPSEATCKNVHFNNAEHVTPHCTSLEISE 449
>PGLR_MALDO (P48978) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 460
Score = 206 bits (525), Expect = 9e-53
Identities = 132/408 (32%), Positives = 205/408 (49%), Gaps = 60/408 (14%)
Query: 92 KPKVDPPSTPPSNDYNGGSSTM------FNVLDFGAKGDGRTDDTKAFQATCAMACKVEA 145
+P+ S+ N GG +T +V DFGAKG+G DDT+AF AC
Sbjct: 71 RPRTQLFSSRKLNTITGGIATSSAPAKTISVDDFGAKGNG-ADDTQAFVKAWKAACSSSG 129
Query: 146 STMVVPAAYVFYVGPISFSGPYCKPNIVFQLDGTIIAPTNSNAWGGGLLQWLEFSKLVGI 205
+ ++V + V PI FSGP CK + Q+ GTI A + + + + WL F + +
Sbjct: 130 AMVLVVPQKNYLVRPIEFSGP-CKSQLTLQIYGTIEASEDRSIYKD-IDHWLIFDNVQNL 187
Query: 206 TIQGKGVIDGRGSVWWQDFPYDDPIDDEEKLIVPLNHTQKPSLPVQNEMGRQMPAVKPTA 265
+ G G I+G G++WW++ + KP P PTA
Sbjct: 188 LVVGPGTINGNGNIWWKN-----------------SCKIKPQPP--------CGTYAPTA 222
Query: 266 LRFYGSFSPTVTGITIQNSPQCHLKFDNCNGVLVHDVSISSPGDSPNTDGIHLQNSKDVL 325
+ F + V + IQ++ Q H+ F NC V +++++P DSPNTDGIH+ N++++
Sbjct: 223 VTFNRCNNLVVKNLNIQDAQQIHVIFQNCINVQASCLTVTAPEDSPNTDGIHVTNTQNIT 282
Query: 326 IHSSKMAC-----------------------GHGISIGSLGKDNTRACVSNITIRDVNMH 362
I SS + GHGISIGSLG+D + VS + + +
Sbjct: 283 ISSSVIGTGDDCISIVSGSQRVQATDITCGPGHGISIGSLGEDGSEDHVSGVFVNGAKLS 342
Query: 363 NTMNGVRIKTWQGGSGSVQGVLFSNIQVSEVQLPIVIDQFYCDKRS--CTNHTSAVALAG 420
T NG+RIKTW+GGSGS ++F N+Q+++V PI+IDQ YCD ++ C SAV +
Sbjct: 343 GTSNGLRIKTWKGGSGSATNIVFQNVQMNDVTNPIIIDQNYCDHKTKDCKQQKSAVQVKN 402
Query: 421 INYEGIKGT-YTVKPVHFACSDSLPCVDVSLTSVELKPVQEQYHLYEP 467
+ Y+ I+GT + + CS S+PC + L SV+L+ + + + +P
Sbjct: 403 VLYQNIRGTSASGDAITLNCSQSVPCQGIVLQSVQLQNGRAECNNVQP 450
>PGLR_PRUPE (P48979) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 393
Score = 191 bits (485), Expect = 4e-48
Identities = 128/373 (34%), Positives = 183/373 (48%), Gaps = 58/373 (15%)
Query: 110 SSTMFNVLDFGAKGDGRTDDTKAFQATCAMACKVEASTMVVPAAYVFYVGPISFSGPYCK 169
S +NV GAK DG+TD TKAF + A AC ++ A F++ + FSGP CK
Sbjct: 24 SPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYVPAGTFFLRDVVFSGP-CK 82
Query: 170 PN-IVFQLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFPYDD 228
N I F++ GT++AP++ G W+ F + G+TI G G++DG+G+ W
Sbjct: 83 NNAITFRIAGTLVAPSDYRVIGNAA-NWIFFHHVNGVTISG-GILDGQGTALWA------ 134
Query: 229 PIDDEEKLIVPLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFSPTVTGITIQNSPQCH 288
+ G P+ T L F S + V+G+ NS H
Sbjct: 135 ---------------------CKACHGESCPS-GATTLGFSDSNNIVVSGLASLNSQMFH 172
Query: 289 LKFDNCNGVLVHDVSISSPGDSPNTDGIHLQNSKDVLIHSSKMACG-------------- 334
+ ++ V + V +S G+SPNTDGIH+Q S V I +SK+A G
Sbjct: 173 IVINDFQNVQMQGVRVSRSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLW 232
Query: 335 ---------HGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTW-QGGSGSVQGVL 384
HGISIGSLGK+ A V N+T++ V T NG+RIK+W + +G + +L
Sbjct: 233 IEGVACGPGHGISIGSLGKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNIL 292
Query: 385 FSNIQVSEVQLPIVIDQFYC-DKRSCTNHTSAVALAGINYEGIKGT-YTVKPVHFACSDS 442
F + + V+ PIVIDQ YC D + C S V ++ + YE I GT T V F CS
Sbjct: 293 FQHATMVNVENPIVIDQHYCPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPK 352
Query: 443 LPCVDVSLTSVEL 455
PC ++ L V+L
Sbjct: 353 HPCREIKLEDVKL 365
>PGLT_MAIZE (P35339) Exopolygalacturonase precursor (EC 3.2.1.67)
(ExoPG) (Pectinase) (Galacturan
1,4-alpha-galacturonidase)
Length = 410
Score = 173 bits (439), Expect = 8e-43
Identities = 110/375 (29%), Positives = 180/375 (47%), Gaps = 56/375 (14%)
Query: 109 GSSTMFNVLDFGAKGDGRTDDTKAFQATCAMACKVEASTMVVPAAYVFYVGPISFSGPYC 168
G F++ GA G+G+TD TKA Q A AC ++ F VGP++F+GP C
Sbjct: 36 GPGGSFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGPLNFTGP-C 94
Query: 169 KPNIVFQLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFPYDD 228
K ++ Q++G ++A T+ + + W+E ++ + I GKG +DG+G W
Sbjct: 95 KGDVTIQVNGNLLATTDLSQYKDHG-NWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVK 153
Query: 229 PIDDEEKLIVPLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFSPTVTGITIQNSPQCH 288
D + + P +L + V+GIT+ NS H
Sbjct: 154 KYDCK---------------------------ILPNSLVMDFVNNGEVSGITLLNSKFFH 186
Query: 289 LKFDNCNGVLVHDVSISSPGDSPNTDGIHLQNSKDVLIHSSKMAC--------------- 333
+ C +L+ DV++++PGDSPNTDGIH+ +S V I ++ +
Sbjct: 187 MNMYKCKDMLIKDVNVTAPGDSPNTDGIHMGDSSGVTITNTVIGVGDDCISIGPGTSKVN 246
Query: 334 --------GHGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQGGSG--SVQGV 383
GHGISIGSLG+ V++I ++D + T NGVRIK ++ + + +
Sbjct: 247 ITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTANGVRIKAYEDAASVLTASKI 306
Query: 384 LFSNIQVSEVQLPIVIDQFYCDKRSCT-NHTSAVALAGINYEGIKGT-YTVKPVHFACSD 441
+ NI++ + PI+ID YC + CT N S V + + ++ I GT T + V+ C+
Sbjct: 307 HYENIKMEDSGYPIIIDMKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPEAVNLLCTA 366
Query: 442 SLPCVDVSLTSVELK 456
+PC V++ V +K
Sbjct: 367 KIPCTGVTMDDVNIK 381
>PGLR_MAIZE (P26216) Exopolygalacturonase precursor (EC 3.2.1.67)
(ExoPG) (Pectinase) (Galacturan
1,4-alpha-galacturonidase)
Length = 410
Score = 167 bits (423), Expect = 6e-41
Identities = 108/375 (28%), Positives = 178/375 (46%), Gaps = 56/375 (14%)
Query: 109 GSSTMFNVLDFGAKGDGRTDDTKAFQATCAMACKVEASTMVVPAAYVFYVGPISFSGPYC 168
G F++ GA G+G+TD TKA Q A AC ++ F VG ++F+GP C
Sbjct: 36 GPGGSFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGP-C 94
Query: 169 KPNIVFQLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFPYDD 228
K ++ Q+DG ++A T+ + + W+E ++ + I GKG +DG+G W
Sbjct: 95 KGDVTIQVDGNLLATTDLSQYKDHG-NWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTK 153
Query: 229 PIDDEEKLIVPLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFSPTVTGITIQNSPQCH 288
D + + P +L + V+G+T+ NS H
Sbjct: 154 KYDCK---------------------------ILPNSLVMDFVNNGEVSGVTLLNSKFFH 186
Query: 289 LKFDNCNGVLVHDVSISSPGDSPNTDGIHLQNSKDVLIHSSKMAC--------------- 333
+ C +L+ DV++++PGDSPNTDGIH+ +S + I ++ +
Sbjct: 187 MNMYRCKDMLIKDVTVTAPGDSPNTDGIHMGDSSGITITNTVIGVGDDCISIGPGTSKVN 246
Query: 334 --------GHGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQGGSG--SVQGV 383
GHGISIGSLG+ V++I ++D + TM GVRIK ++ + +V +
Sbjct: 247 ITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAYEDAASVLTVSKI 306
Query: 384 LFSNIQVSEVQLPIVIDQFYCDKRSCT-NHTSAVALAGINYEGIKGT-YTVKPVHFACSD 441
+ NI++ + PI ID YC + CT N S V + + ++ I GT T + V C+
Sbjct: 307 HYENIKMEDSANPIFIDMKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPEAVSLLCTA 366
Query: 442 SLPCVDVSLTSVELK 456
+PC V++ V ++
Sbjct: 367 KVPCTGVTMDDVNVE 381
>PGL2_PLAAC (Q6H9K0) Exopolygalacturonase precursor (EC 3.2.1.67)
(Pollen allergen Pla a 2) (Fragment)
Length = 377
Score = 166 bits (421), Expect = 1e-40
Identities = 117/376 (31%), Positives = 175/376 (46%), Gaps = 65/376 (17%)
Query: 110 SSTMFNVLDFGAKGDGRTDDT--KAFQATCAMACKVEASTMVVPAAYVFYVGPISFSGPY 167
S ++FNV D+GAKG G KA++A CA ST+++P + +G ++ GP
Sbjct: 6 SGSVFNVNDYGAKGAGDISQAVMKAWKAACASQ---GPSTVLIPKGN-YNMGEVAMQGPC 61
Query: 168 CKPNIVFQLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFPYD 227
I FQ+DG + AP + + + W+ F ++ G+T+ G G +DG+G W D
Sbjct: 62 KGSKIGFQIDGVVKAPADPSKFKSD--GWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCD 119
Query: 228 DPIDDEEKLIVPLNHTQKPSLPVQNEMGRQMPAVKPTA--LRFYGSFSPTVTGITIQNSP 285
+ P K A LRF V IT NS
Sbjct: 120 -----------------------------KNPNCKHAAMNLRFDFLKHAMVRDITSLNSK 150
Query: 286 QCHLKFDNCNGVLVHDVSISSPGDSPNTDGIHLQNSKDVLIHSSKMACG----------- 334
H+ C + V++++PG S NTDGIH+ SK V I ++K+A G
Sbjct: 151 MFHINVLECEDITFQHVTVTAPGTSINTDGIHVGISKGVTITNTKIATGDDCISIGPGSQ 210
Query: 335 ------------HGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQGG-SGSVQ 381
HGISIGSLG+ N V IT++ TMNGVR+KTW G+
Sbjct: 211 NVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKGCTFSGTMNGVRVKTWPNSPPGAAT 270
Query: 382 GVLFSNIQVSEVQLPIVIDQFYCDKRSCTNHT-SAVALAGINYEGIKGTYTVK-PVHFAC 439
+ F ++ ++ VQ P+++DQ YC C+ S + L+ IN+ I+GT T K V AC
Sbjct: 271 DLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPSRIKLSNINFNNIRGTSTGKVAVVIAC 330
Query: 440 SDSLPCVDVSLTSVEL 455
S +PC ++ + + L
Sbjct: 331 SHGMPCSNMKIGEINL 346
>PGL2_ARATH (P49063) Exopolygalacturonase clone GBGA483 precursor
(EC 3.2.1.67) (ExoPG) (Pectinase) (Galacturan
1,4-alpha-galacturonidase)
Length = 444
Score = 166 bits (421), Expect = 1e-40
Identities = 114/363 (31%), Positives = 169/363 (46%), Gaps = 58/363 (15%)
Query: 120 GAKGDGRTDDTKAFQATCAMACKVEASTMVVPAAYVFYVGPISFSGPYCKPNIVFQLDGT 179
GAKGD +TDD+ AF A AC ST+ VP + V + F GP CK + +L+G
Sbjct: 76 GAKGDSKTDDSAAFAAAWKEACAA-GSTITVPKGE-YMVESLEFKGP-CKGPVTLELNGN 132
Query: 180 IIAPTNSNAWGGGLLQWLEFSKLVGITIQG-KGVIDGRGSVWWQDFPYDDPIDDEEKLIV 238
AP W++F + T+ G K + DG+GS+ W+ +D + +
Sbjct: 133 FKAPATVKTTKPHA-GWIDFENIADFTLNGNKAIFDGQGSLAWKA---NDCAKTGKCNSL 188
Query: 239 PLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFSPTVTGITIQNSPQCHLKFDNCNGVL 298
P+N +RF G + + IT NS H+ NC +
Sbjct: 189 PIN------------------------IRFTGLTNSKINSITSTNSKLFHMNILNCKNIT 224
Query: 299 VHDVSISSPGDSPNTDGIHLQNS-----------------------KDVLIHSSKMACGH 335
+ D+ I +P +S NTDGIH+ S +++++ + + GH
Sbjct: 225 LSDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGH 284
Query: 336 GISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQGG-SGSVQGVLFSNIQVSEVQ 394
GISIGSLG+ V +T+R + NT NGVRIKTW G G +LF +I + V
Sbjct: 285 GISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVS 344
Query: 395 LPIVIDQFYCDKRSC-TNHTSAVALAGINYEGIKGTYTVK-PVHFACSDSLPCVDVSLTS 452
LP++IDQ YC C S V L+ + +GIKGT K V CS +PC +++L+
Sbjct: 345 LPVLIDQEYCPYGHCKAGVPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSD 404
Query: 453 VEL 455
+ L
Sbjct: 405 INL 407
>PGLS_MAIZE (P35338) Exopolygalacturonase precursor (EC 3.2.1.67)
(ExoPG) (Pectinase) (Galacturan
1,4-alpha-galacturonidase)
Length = 410
Score = 165 bits (418), Expect = 2e-40
Identities = 106/375 (28%), Positives = 177/375 (46%), Gaps = 56/375 (14%)
Query: 109 GSSTMFNVLDFGAKGDGRTDDTKAFQATCAMACKVEASTMVVPAAYVFYVGPISFSGPYC 168
G F++ GA G+G+TD TKA Q A AC ++ F VG ++F+GP C
Sbjct: 36 GPGGSFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGP-C 94
Query: 169 KPNIVFQLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFPYDD 228
K ++ Q+DG ++A T+ + + W+E ++ + I GKG +DG+G W
Sbjct: 95 KGDVTIQVDGNLLATTDLSQYKEHG-NWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTK 153
Query: 229 PIDDEEKLIVPLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFSPTVTGITIQNSPQCH 288
D + + P +L + V+G+T+ NS H
Sbjct: 154 KYDCK---------------------------ILPNSLVMDFVNNGEVSGVTLLNSKFFH 186
Query: 289 LKFDNCNGVLVHDVSISSPGDSPNTDGIHLQNSKDVLIHSSKMAC--------------- 333
+ C +L+ DV++++PGDSPNTDGIH+ +S + I ++ +
Sbjct: 187 MNMYQCKNMLIKDVTVTAPGDSPNTDGIHMGDSSGITITNTVIGVGDDCISIGPGTSKVN 246
Query: 334 --------GHGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQGGSG--SVQGV 383
GHGISIGSLG+ V++I ++D + TM GVRIK ++ + +V +
Sbjct: 247 ITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAYEDAASVLTVSKI 306
Query: 384 LFSNIQVSEVQLPIVIDQFYCDKRSCT-NHTSAVALAGINYEGIKGT-YTVKPVHFACSD 441
+ NI++ + PI ID YC + CT N S V + + ++ I GT T + + C+
Sbjct: 307 HYENIKMEDSANPIFIDMKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPEAISLLCTA 366
Query: 442 SLPCVDVSLTSVELK 456
+PC ++ V ++
Sbjct: 367 KVPCTGATMDDVNVE 381
>PGL1_ARATH (P49062) Exopolygalacturonase clone GBGE184 precursor
(EC 3.2.1.67) (ExoPG) (Pectinase) (Galacturan
1,4-alpha-galacturonidase)
Length = 422
Score = 164 bits (416), Expect = 4e-40
Identities = 115/376 (30%), Positives = 176/376 (46%), Gaps = 65/376 (17%)
Query: 107 NGGSSTMFNVLDFGAKGDGRTDDTKAFQATCAMACKVEA-STMVVPAAYVFYVGPISFSG 165
N G+ T++++ FGA GDG T+ KAF T C +T++VP F GP+ F+G
Sbjct: 45 NSGNPTVYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPVPATLLVPKG-TFLAGPVIFAG 103
Query: 166 PYCKPNIVFQLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFP 225
P CK + + GTIIA T+ G +W F ++ + + G G G+G W+
Sbjct: 104 P-CKSKVTVNVIGTIIATTS----GYATPEWFLFERVDNLVLTGTGTFHGKGEAVWK--- 155
Query: 226 YDDPIDDEEKLIVPLNHTQKPSLPVQNEMGRQMPA-VKPTALRFYGSFSPTVTGITIQNS 284
+ G+++ + PT+L+F + + GI+ N+
Sbjct: 156 -------------------------ADGCGKKVQCNLPPTSLKFRNMKNVEINGISSVNA 190
Query: 285 PQCHLKFDNCNGVLVHDVSISSPGDSPNTDGIHLQNSKDVLIHSSKMAC----------- 333
H+ V + ++ +++P +SPNTDGIHL N+ +V I S +A
Sbjct: 191 KAFHMFLVKTENVNIQNIKLTAPAESPNTDGIHLSNADNVSILDSTIATGDDCVSVGRGS 250
Query: 334 ------------GHGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQGGSGS-V 380
GHG+S+GSLGK VS I + + M T NG+RIKTW G S
Sbjct: 251 NNVTVERVICGPGHGLSVGSLGKYKNEEDVSGIHVNNCTMIETDNGLRIKTWGGSDPSKA 310
Query: 381 QGVLFSNIQVSEVQLPIVIDQFYCDKRSCTNHTSAVALAGINYEGIKGTYTVKP-VHFAC 439
+ F NI + V+ PI+IDQ Y + S VA++ I ++ I+GT K V C
Sbjct: 311 VDIKFENIIMQSVKNPIIIDQNYGSR----GGDSQVAISDILFKNIRGTTITKDVVQIMC 366
Query: 440 SDSLPCVDVSLTSVEL 455
S S+PC V++ V L
Sbjct: 367 SKSVPCQGVNVVDVNL 382
>PGLR_GOSHI (Q39786) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 407
Score = 163 bits (413), Expect = 8e-40
Identities = 115/368 (31%), Positives = 175/368 (47%), Gaps = 58/368 (15%)
Query: 116 VLDFGAKGDGRTDDTKAFQATCAMAC-KVEASTMVVPAAYVFYVGPISFSGPYCKPNIVF 174
V FGAK DG+TD +K F AC V ST+V+P + + ++ GP CK I
Sbjct: 32 VAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKG-TYLLSKVNLEGP-CKAPIEI 89
Query: 175 QLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFPYDDPIDDEE 234
+ GTI AP + +A+ W+ F + + G G+ DG+GS+ ++ ++
Sbjct: 90 NVQGTIQAPADPSAFKDP--NWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCEN------ 141
Query: 235 KLIVPLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFSPTVTGITIQNSPQCHLKFDNC 294
R+ + P +RF + + IT ++S H+ C
Sbjct: 142 ---------------------REFRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVFAC 180
Query: 295 NGVLVHDVSISSPGDSPNTDGIHLQNSKDVLIHSS---------------------KMAC 333
+ + + I +P +SPNTDGIH+ S+ V I +S ++ C
Sbjct: 181 KNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITC 240
Query: 334 --GHGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQG-GSGSVQGVLFSNIQV 390
GHGISIGSLGK V I I + + NT NG RIKTW G G+V + F +I +
Sbjct: 241 GPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITM 300
Query: 391 SEVQLPIVIDQFYCDKRSC-TNHTSAVALAGINYEGIKGTYTV-KPVHFACSDSLPCVDV 448
+ V PI+IDQ YC C N S V L+ I+++ I+GT + + + F CS S PC +V
Sbjct: 301 NNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNV 360
Query: 449 SLTSVELK 456
L +++K
Sbjct: 361 ELADIDIK 368
>PGLR_GOSBA (Q39766) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 407
Score = 162 bits (409), Expect = 2e-39
Identities = 114/368 (30%), Positives = 175/368 (46%), Gaps = 58/368 (15%)
Query: 116 VLDFGAKGDGRTDDTKAFQATCAMAC-KVEASTMVVPAAYVFYVGPISFSGPYCKPNIVF 174
V FGAK DG+TD +K F AC V ST+V+P + + ++ GP CK I
Sbjct: 32 VAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKG-TYLLSKVNLEGP-CKAPIEI 89
Query: 175 QLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFPYDDPIDDEE 234
+ GTI AP + +A+ W+ F + + G G+ DG+GS+ ++ ++
Sbjct: 90 NVQGTIQAPADPSAFKDP--NWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCEN------ 141
Query: 235 KLIVPLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFSPTVTGITIQNSPQCHLKFDNC 294
R+ + P +RF + + IT ++S H+ C
Sbjct: 142 ---------------------REFRSKLPVNIRFDFVTNALIQDITSKDSKLFHINVFAC 180
Query: 295 NGVLVHDVSISSPGDSPNTDGIHLQNSKDVLIHSS---------------------KMAC 333
+ + + I +P +SPNTDGIH+ S+ V I +S ++ C
Sbjct: 181 KNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITC 240
Query: 334 --GHGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQG-GSGSVQGVLFSNIQV 390
GHGISIGSLGK V I I + + NT NG RIKTW G G+V + F +I +
Sbjct: 241 GPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITM 300
Query: 391 SEVQLPIVIDQFYCDKRSC-TNHTSAVALAGINYEGIKGTYTV-KPVHFACSDSLPCVDV 448
+ V PI+IDQ YC C N S V L+ I+++ I+GT + + + F CS S PC +V
Sbjct: 301 NNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNV 360
Query: 449 SLTSVELK 456
L ++++
Sbjct: 361 ELADIDIQ 368
>PGLR_MEDSA (Q40312) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 421
Score = 157 bits (397), Expect = 6e-38
Identities = 114/381 (29%), Positives = 178/381 (45%), Gaps = 58/381 (15%)
Query: 105 DYNGGSSTMFNVLDFGAKGDGRTDD--TKAFQATCAMACKVEASTMVVPAAYVFYVGPIS 162
D+ S + ++ FG K + T A+ CA A+ +V+PA + + I
Sbjct: 16 DFCAAQSGVLDISKFGGKPNSDIGQALTSAWNEACA---STTAAKIVIPAG-TYQLNGIE 71
Query: 163 FSGPYCKPNIVFQLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQ 222
GP CK I Q+DGTI AP + + G QW +F + +T+ GKGV DG+G+ +
Sbjct: 72 LKGP-CKAPIELQVDGTIQAPADPSVIKG-TEQWFKFLYMDHLTLSGKGVFDGQGATVY- 128
Query: 223 DFPYDDPIDDEEKLIVPLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFSPTVTGITIQ 282
K + P G+ + F + V G+T +
Sbjct: 129 ----------------------KKAAPASAWSGKNSNSKVFMNFGFNFVNNSIVRGVTSK 166
Query: 283 NSPQCHLKFDNCNGVLVHDVSISSPGDSPNTDGIHLQNSKDVLIHSSKMACG-------- 334
+S H+ C + +I++PGDSPNTDGIH+ S DV I ++ + G
Sbjct: 167 DSKNFHVMVFGCKNITFDGFTITAPGDSPNTDGIHMGKSTDVKILNTNIGTGDDCVSIGD 226
Query: 335 ---------------HGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQGGSG- 378
HG+S+GSLGK T V IT+++ + T NGVRIKTW G
Sbjct: 227 GSKQITVQGVNCGPGHGLSVGSLGKFTTEENVEGITVKNCTLTATDNGVRIKTWPDAPGT 286
Query: 379 -SVQGVLFSNIQVSEVQLPIVIDQFYCDKRSCT-NHTSAVALAGINYEGIKGTY-TVKPV 435
+V + F +I ++ V+ P++IDQ Y C+ + S + L+ I+++ +KGT T + V
Sbjct: 287 ITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCSKKNPSKIKLSKISFKNVKGTSGTAEGV 346
Query: 436 HFACSDSLPCVDVSLTSVELK 456
CS ++PC V L +V+LK
Sbjct: 347 VLICSSAVPCDGVELNNVDLK 367
>PGLR_OENOR (P24548) Exopolygalacturonase (EC 3.2.1.67) (ExoPG)
(Pectinase) (Galacturan 1,4-alpha-galacturonidase)
(Fragment)
Length = 362
Score = 145 bits (366), Expect = 2e-34
Identities = 112/354 (31%), Positives = 160/354 (44%), Gaps = 63/354 (17%)
Query: 130 TKAFQATCAMACKVEASTMVVPAAYVFYVGPISFSGPYCKPNIVFQLDGTIIAPTNSNAW 189
T A++ CA A ST++VP F VG I+ GP CK +I QL GT+ AP + +
Sbjct: 7 TTAWKEACASA---SPSTILVPKGN-FAVGLITLEGP-CKSSIGLQLQGTLKAPADPSKI 61
Query: 190 GGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFPYDDPIDDEEKLIVPLNHTQKPSLP 249
G L W+ +K+ +TI G GV DG+G W
Sbjct: 62 KG--LGWINLNKIDLLTIFGGGVFDGQGKSAW---------------------------- 91
Query: 250 VQNEMGRQMPAVKPTA--LRFYGSFSPTVTGITIQNSPQCHLKFDNCNGVLVHDVSISSP 307
VQN+ + P K + LR Y + + +T +S H+ C + IS+
Sbjct: 92 VQNDCHKNGPICKTLSMNLRLYAVTNSILRDVTTLDSKNFHVNVIGCKNLTFERFKISAA 151
Query: 308 GDSPNTDGIHL-----------------------QNSKDVLIHSSKMACGHGISIGSLGK 344
S NTDGIH+ SK++ I + GHGIS+GSLG+
Sbjct: 152 ETSINTDGIHIGRSDGVNIINTEIKTGDDCISLGDGSKNINITNITCGPGHGISVGSLGR 211
Query: 345 DNTRACVSNITIRDVNMHNTMNGVRIKTW-QGGSGSVQGVLFSNIQVSEVQLPIVIDQFY 403
V I +++ + + NGVRIKTW + G + F +I ++ V PI+IDQ Y
Sbjct: 212 YKNEESVVGIYVKNCTITGSQNGVRIKTWPKSEPGEASEMHFQDITMNSVGTPILIDQGY 271
Query: 404 CDKRSCTNHT-SAVALAGINYEGIKGTYTVK-PVHFACSDSLPCVDVSLTSVEL 455
C CT S+V L+ I+++ IKGT T K V CS S PC V L ++L
Sbjct: 272 CPYNQCTAEVPSSVKLSKISFKNIKGTSTTKEAVKLVCSKSFPCNGVELADIDL 325
>PGLR_TOBAC (Q05967) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 396
Score = 134 bits (337), Expect = 5e-31
Identities = 105/377 (27%), Positives = 166/377 (43%), Gaps = 66/377 (17%)
Query: 108 GGSST-MFNVLDFGAKGDGRTDDT--KAFQATCAMACKVEASTMVVPAAYVFYVGPISFS 164
G S T +F++ +GA + + AF+ C ST+V+P F + +
Sbjct: 20 GESQTGVFDITKYGANSNADISEALLNAFKEACQ---STSPSTIVIPKG-TFTMNQVKLE 75
Query: 165 GPYCKPNIVFQLDGTIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDF 224
GP CK + Q+ T+ AP++ + G +WL +KL T+ G G++DG+ + W+
Sbjct: 76 GP-CKSPLELQIQATLKAPSDPSQLKVG--EWLTVNKLDQFTMSGGGILDGQAAAAWEC- 131
Query: 225 PYDDPIDDEEKLIVPLNHTQKPSLPVQNEMGRQMPAVKPTALRFYGSFSPTVTGITIQNS 284
Q++ ++P L F + T+ IT +S
Sbjct: 132 -------------------------KQSKKCNKLP----NNLSFNSLTNSTIKDITTLDS 162
Query: 285 PQCHLKFDNCNGVLVHDVSISSPGDSPNTDGIHLQNSKDVLIHSS--------------- 329
H+ + C + ++S+P +SPNTDGIH+ S V I S
Sbjct: 163 KSFHVNVNQCKNLTFIRFNVSAPANSPNTDGIHVSRSSSVNITDSNFSTGDDCISVGDET 222
Query: 330 ------KMAC--GHGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQGG-SGSV 380
++ C GHGIS+GSLG + V + +R+ NT NGVRIKTW G V
Sbjct: 223 EQLYITRVTCGPGHGISVGSLGGNPDEKPVVGVFVRNCTFTNTDNGVRIKTWPASHPGVV 282
Query: 381 QGVLFSNIQVSEVQLPIVIDQFYCDKRSCTNH-TSAVALAGINYEGIKGT-YTVKPVHFA 438
V F +I V V P+VIDQ YC C S V ++ ++++ IKGT T V
Sbjct: 283 NDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLL 342
Query: 439 CSDSLPCVDVSLTSVEL 455
S +PC + + +++
Sbjct: 343 RSKGVPCEGIEVGDIDI 359
>PGLR_BRANA (P35337) Polygalacturonase precursor (EC 3.2.1.15) (PG)
(Pectinase)
Length = 397
Score = 131 bits (330), Expect = 4e-30
Identities = 100/366 (27%), Positives = 156/366 (42%), Gaps = 60/366 (16%)
Query: 119 FGAKGDGRTDDTKAFQATCAMACKVEASTMVVPAAYVFYVGPISFSGPYCKPNIVFQLDG 178
F A G +D T A AC+ A + V+ F +G +GP CK I F L G
Sbjct: 25 FTAGGPPNSDITAAVLKAFTSACQAPAPSQVLIPKGDFKLGETVMTGP-CKSPIEFTLQG 83
Query: 179 TIIAPTNSNAWGGGLLQWLEFSKLVGITIQGKGVIDGRGSVWWQDFPYDDPIDDEEKLIV 238
+ S G +W+ F K+ G + G G DG G+ W+
Sbjct: 84 NVKTDGGSTQ---GKDRWVVFEKINGFKLNGGGTFDGEGNAAWK---------------- 124
Query: 239 PLNHTQKPSLPVQNEMGRQMPAVK-PTALRFYGSFSPTVTGITIQNSPQCHLKFDNCNGV 297
N + K P ++RF + + +T ++ H + +
Sbjct: 125 ------------ANNCHKTFECKKLPISVRFDFVDNAEIKDVTSLDAKNFHFNVISGKNM 172
Query: 298 LVHDVSISSPGDSPNTDGIHLQNSKDVLIHSSKMACG----------------------- 334
++ I +P +SPNTDGIHL + V I ++K+A G
Sbjct: 173 TFDNIKIIAPAESPNTDGIHLGRCEGVKILNTKIATGDDCISVGDGMKNLLIEKVVCGPG 232
Query: 335 HGISIGSLGKDNTRACVSNITIRDVNMHNTMNGVRIKTWQGG--SGSVQGVLFSNIQVSE 392
HGIS+GSLG+ V++IT+++ + T NG+RIKTW + + G+ F +I +++
Sbjct: 233 HGISVGSLGRYGWEQDVTDITVKNCTLEGTSNGLRIKTWPSAACTTTAAGIHFEDIILNK 292
Query: 393 VQLPIVIDQFYCDKRSCT-NHTSAVALAGINYEGIKGTYTVK-PVHFACSDSLPCVDVSL 450
V PI+IDQ YC C N S + L I + I+GT K V CS PC +V +
Sbjct: 293 VSNPILIDQEYCPWNQCNKNKPSTIKLVDITFRNIRGTSENKDAVKLLCSKGHPCENVEI 352
Query: 451 TSVELK 456
+ ++
Sbjct: 353 GDINIE 358
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.318 0.135 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,573,125
Number of Sequences: 164201
Number of extensions: 3227250
Number of successful extensions: 15047
Number of sequences better than 10.0: 186
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 83
Number of HSP's that attempted gapping in prelim test: 13426
Number of HSP's gapped (non-prelim): 1090
length of query: 504
length of database: 59,974,054
effective HSP length: 115
effective length of query: 389
effective length of database: 41,090,939
effective search space: 15984375271
effective search space used: 15984375271
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 68 (30.8 bits)
Lotus: description of TM0008.5