BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0143c.1
(197 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g47020 putative peptide chain release factor 262 1e-70
At3g62910 translation releasing factor RF-1 -like protein 103 5e-23
At1g56350 hypothetical protein 96 9e-21
At5g36170 translation releasing factor RF-2 86 1e-17
At1g33330 peptide chain release factor, putative 68 4e-12
At1g35540 auxin response factor, putative 44 4e-05
At3g57190 unknown protein 40 6e-04
At5g10670 putative protein 32 0.17
At4g25190 putative protein 30 1.1
At3g44670 disease resistance protein homlog 29 1.8
At3g44480 disease resistance protein -like 29 1.8
At5g53670 putative protein 28 2.4
At2g02060 unknown protein 28 2.4
At3g27200 blue copper protein, putative 28 4.1
At4g30500 unknown protein 27 5.4
At1g79280 hypothetical protein 27 5.4
At5g56210 unknown protein 27 7.0
At3g56600 putative protein 27 7.0
At5g27890 putative protein 27 9.1
At4g36180 receptor protein kinase like protein 27 9.1
>At2g47020 putative peptide chain release factor
Length = 395
Score = 262 bits (669), Expect = 1e-70
Identities = 125/162 (77%), Positives = 153/162 (94%)
Query: 36 SSSGSVGLTNEIMKVDVQLKNEDLKVDTYRSGGSGGQHANTTNSAVRITHIPTGIIVAIQ 95
+S+ SV + + +VDVQL+NEDL++DTYRSGGSGGQHANTTNSAVRI H+PTG++V+IQ
Sbjct: 233 TSAISVAILPQADEVDVQLRNEDLRIDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSIQ 292
Query: 96 DERSQHMNKAKALKILCAKLYEMERLRLHSNRSKLRSEQIGSGDRSERIRTYNFPQGRVT 155
DERSQHMN+AKALK+LCA+LYE+ERLR+ S+RSKLRS+QIGSGDRS RIRTYNFPQGRVT
Sbjct: 293 DERSQHMNRAKALKVLCARLYEIERLRIQSSRSKLRSDQIGSGDRSGRIRTYNFPQGRVT 352
Query: 156 DHRVGITYHSIDDVMQGENLDVFIDALLLREEMDAVASFTSS 197
DHRVGIT+H+I+D+MQGENLD+FIDALLLR+EMDA+ASF+S+
Sbjct: 353 DHRVGITHHAIEDMMQGENLDMFIDALLLRQEMDAIASFSST 394
>At3g62910 translation releasing factor RF-1 -like protein
Length = 422
Score = 103 bits (258), Expect = 5e-23
Identities = 52/131 (39%), Positives = 89/131 (67%), Gaps = 6/131 (4%)
Query: 36 SSSGSVGLTNEIMKVDVQLKNEDLKVDTYRSGGSGGQHANTTNSAVRITHIPTGIIVAIQ 95
+S+ +V + E +V+V + +D+++ + RSGG+GGQ+ N +A+ + H P+GI +
Sbjct: 254 TSTATVAIMPEADEVEVVIDPKDIELTSARSGGAGGQNVNKVETAIDLFHKPSGIRIFCT 313
Query: 96 DERSQHMNKAKALKILCAKLYEMERLRLHSNRSKLRSE---QIGSGDRSERIRTYNFPQG 152
+ER+Q NKA+A ++L AKLYE +++ + K+R+E Q+G+G RSE+IRTYN+
Sbjct: 314 EERTQIRNKARAFQLLRAKLYE---IKVREQQEKIRNERKSQVGTGARSEKIRTYNYKDS 370
Query: 153 RVTDHRVGITY 163
RVTDHR+ + +
Sbjct: 371 RVTDHRLKMNF 381
>At1g56350 hypothetical protein
Length = 524
Score = 96.3 bits (238), Expect = 9e-21
Identities = 51/123 (41%), Positives = 77/123 (62%), Gaps = 2/123 (1%)
Query: 52 VQLKNEDLKVDTYRSGGSGGQHANTTNSAVRITHIPTGIIVAIQDERSQHMNKAKALKIL 111
V++ + DL+++ +RSGG+GGQHANTT+SAVRI HIPTGI Q+ERSQH NKA A+ +L
Sbjct: 366 VEINDSDLRIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNKASAMAVL 425
Query: 112 CAKLYEMERLRLHSNRSKLRSEQIGSGDRSERIRTYNF-PQGRVTDHRVGITYHSIDDVM 170
++L ++E R + ++ ++ + +IRTY P V D R D V+
Sbjct: 426 QSRLDQLEMARQTAMNAQ-HTQSLTEISWGNQIRTYVLHPYRMVKDLRTNYEVSDPDSVL 484
Query: 171 QGE 173
+G+
Sbjct: 485 EGD 487
>At5g36170 translation releasing factor RF-2
Length = 456
Score = 86.3 bits (212), Expect = 1e-17
Identities = 57/145 (39%), Positives = 80/145 (54%), Gaps = 3/145 (2%)
Query: 50 VDVQLKNEDLKVDTYRSGGSGGQHANTTNSAVRITHIPTGIIVAIQDERSQHMNKAKALK 109
V +++ EDL + R+GG GGQ+ N +AVRITHIPTG+ V +ERSQ NK +AL
Sbjct: 310 VGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALI 369
Query: 110 ILCAKLYEMERLRLHSNRSKLRSEQIGSGDRSERIRTYNF-PQGRVTDHRVGITYHSIDD 168
L AKL + + + ++R + + + ++IR Y F P V D R G I
Sbjct: 370 RLKAKLMVIAEEQRATEIKEIRGDAV-KAEWGQQIRNYVFHPYKLVKDVRTGHETSDITS 428
Query: 169 VMQGENLDVFIDALLLREEMDAVAS 193
VM G+ LD FI A L + A+AS
Sbjct: 429 VMDGD-LDPFIKAYLKHKYTLAMAS 452
>At1g33330 peptide chain release factor, putative
Length = 257
Score = 67.8 bits (164), Expect = 4e-12
Identities = 44/112 (39%), Positives = 58/112 (51%), Gaps = 12/112 (10%)
Query: 12 LSLSLSLSLSLSLSLSLSHTHTHSSSSGSVGLTNEIMKV--DVQLKN----------EDL 59
LS LS L L S S SS G G N+ + V DVQ N +
Sbjct: 39 LSDGLSTRLCSILRYSSSDGVNGGSSGGDFGNDNDSVSVVSDVQSPNYLKFTDEELMKQC 98
Query: 60 KVDTYRSGGSGGQHANTTNSAVRITHIPTGIIVAIQDERSQHMNKAKALKIL 111
+++T+R G GGQH N +SAVR+ H+PTGI+ ++RSQH N+A AL L
Sbjct: 99 RLETFRVSGPGGQHRNKRDSAVRLKHLPTGIVAQAVEDRSQHKNRASALNRL 150
>At1g35540 auxin response factor, putative
Length = 661
Score = 44.3 bits (103), Expect = 4e-05
Identities = 37/85 (43%), Positives = 45/85 (52%), Gaps = 21/85 (24%)
Query: 1 SLSLSLSLSLSLSLSLSLS------------LSLSLSLSLSHTHTHSSSSGSVGLTNEIM 48
SLSLSLSLSLSLSLSLSLS LSLSLSLSL +H +S+ + I
Sbjct: 69 SLSLSLSLSLSLSLSLSLSPFSLFSLFSLSPLSLSLSLSLLLSHVEASTREELNELQPIC 128
Query: 49 K---------VDVQLKNEDLKVDTY 64
+ +QLK E+ +TY
Sbjct: 129 DFPSKLQCRVIAIQLKVENNSDETY 153
Score = 37.7 bits (86), Expect = 0.004
Identities = 24/29 (82%), Positives = 24/29 (82%)
Query: 1 SLSLSLSLSLSLSLSLSLSLSLSLSLSLS 29
SLSL LSLSLSLSLSLSLSLSLSLS
Sbjct: 59 SLSLYSFSLLSLSLSLSLSLSLSLSLSLS 87
Score = 29.3 bits (64), Expect = 1.4
Identities = 19/28 (67%), Positives = 20/28 (70%)
Query: 2 LSLSLSLSLSLSLSLSLSLSLSLSLSLS 29
+ L SLSL LSLSLSLSLSLSLS
Sbjct: 54 IELVSSLSLYSFSLLSLSLSLSLSLSLS 81
>At3g57190 unknown protein
Length = 406
Score = 40.4 bits (93), Expect = 6e-04
Identities = 33/113 (29%), Positives = 59/113 (52%), Gaps = 5/113 (4%)
Query: 81 VRITHIPTGIIVAIQDERSQHMNKAKALKILCAKLYEMERLRLHSNRSKLRSEQIGSGDR 140
V I HIP+G+ + ER++ N+ KAL L AKL + + + S+ +K+ S+ I +
Sbjct: 297 VCIHHIPSGVTLQSSGERNRFANRIKALNRLKAKLLVIAKEQKVSDVNKIDSKNI--LEP 354
Query: 141 SERIRTY-NFPQGRVTDHRVGITYHSIDDVMQGENLDVFIDA-LLLREEMDAV 191
E R+Y + V D + G+ + V+ G N+ + A + +R +DA+
Sbjct: 355 REETRSYVSKGHKMVVDRKTGLEILDLKSVLDG-NIGPLLGAHISMRRSIDAI 406
>At5g10670 putative protein
Length = 218
Score = 32.3 bits (72), Expect = 0.17
Identities = 20/35 (57%), Positives = 23/35 (65%)
Query: 4 LSLSLSLSLSLSLSLSLSLSLSLSLSHTHTHSSSS 38
LSLSLSLSLSLSLSLS L + + H +S S
Sbjct: 184 LSLSLSLSLSLSLSLSPRLHRASGIGHREDCNSVS 218
Score = 30.8 bits (68), Expect = 0.48
Identities = 20/34 (58%), Positives = 21/34 (60%)
Query: 8 LSLSLSLSLSLSLSLSLSLSLSHTHTHSSSSGSV 41
LSLSLSLSLSLSLSLS L + H SV
Sbjct: 184 LSLSLSLSLSLSLSLSPRLHRASGIGHREDCNSV 217
Score = 30.4 bits (67), Expect = 0.63
Identities = 20/35 (57%), Positives = 22/35 (62%)
Query: 6 LSLSLSLSLSLSLSLSLSLSLSLSHTHTHSSSSGS 40
LSLSLSLSLSLSLSLS L + H +S S
Sbjct: 184 LSLSLSLSLSLSLSLSPRLHRASGIGHREDCNSVS 218
Score = 29.3 bits (64), Expect = 1.4
Identities = 16/16 (100%), Positives = 16/16 (100%)
Query: 2 LSLSLSLSLSLSLSLS 17
LSLSLSLSLSLSLSLS
Sbjct: 184 LSLSLSLSLSLSLSLS 199
Score = 27.7 bits (60), Expect = 4.1
Identities = 15/15 (100%), Positives = 15/15 (100%)
Query: 1 SLSLSLSLSLSLSLS 15
SLSLSLSLSLSLSLS
Sbjct: 185 SLSLSLSLSLSLSLS 199
>At4g25190 putative protein
Length = 443
Score = 29.6 bits (65), Expect = 1.1
Identities = 24/79 (30%), Positives = 34/79 (42%)
Query: 11 SLSLSLSLSLSLSLSLSLSHTHTHSSSSGSVGLTNEIMKVDVQLKNEDLKVDTYRSGGSG 70
S ++S S+SL +SL+ SLS + + SSSS + +M Q + SG
Sbjct: 18 SRTISSSISLPVSLNASLSSSTSSSSSSSPSNSSKRVMITRSQSTTRSSRPIGSSDSKSG 77
Query: 71 GQHANTTNSAVRITHIPTG 89
NSA R I G
Sbjct: 78 ENIIPARNSASRSQEINNG 96
>At3g44670 disease resistance protein homlog
Length = 1199
Score = 28.9 bits (63), Expect = 1.8
Identities = 19/36 (52%), Positives = 22/36 (60%)
Query: 3 SLSLSLSLSLSLSLSLSLSLSLSLSLSHTHTHSSSS 38
S S LS S + SLSLSLS S+SLSH + S S
Sbjct: 1151 SSSPDLSPESSRAFSLSLSLSPSISLSHELVNDSIS 1186
>At3g44480 disease resistance protein -like
Length = 1220
Score = 28.9 bits (63), Expect = 1.8
Identities = 19/36 (52%), Positives = 22/36 (60%)
Query: 3 SLSLSLSLSLSLSLSLSLSLSLSLSLSHTHTHSSSS 38
S S LS S + SLSLSLS S+SLSH + S S
Sbjct: 1172 SSSPDLSPESSRAFSLSLSLSPSISLSHELVNDSIS 1207
>At5g53670 putative protein
Length = 244
Score = 28.5 bits (62), Expect = 2.4
Identities = 33/135 (24%), Positives = 57/135 (41%), Gaps = 5/135 (3%)
Query: 1 SLSLSLSLSLSLSLSLSLSLSLSLSLSLSHTHTHSSSSGS-VGLTNEIMKVDVQLKNEDL 59
S+ + L+ L+LS SL L + SLS+ + SGS E + + + L + DL
Sbjct: 5 SVGYKVYLTTYLTLSFSLFSVLGYTSSLSNVDSIPMLSGSNFSEWKEHLLLVLALMDLDL 64
Query: 60 KVDTYRSGG----SGGQHANTTNSAVRITHIPTGIIVAIQDERSQHMNKAKALKILCAKL 115
+ T R +N + + IP G + D+ + + +L+ AK
Sbjct: 65 SLMTERPSSPKELKHWDRSNRVSIMIMKIRIPQGFRGVVPDDVTTAKDFLASLENFFAKN 124
Query: 116 YEMERLRLHSNRSKL 130
E ER R+ + S +
Sbjct: 125 EEAERSRVQAESSSM 139
>At2g02060 unknown protein
Length = 626
Score = 28.5 bits (62), Expect = 2.4
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 8/66 (12%)
Query: 22 LSLSLSLSHTHTHSSSSGSVGLTNEIMKVDVQLKNEDLKVDTYRSGGSGGQHANTTNSAV 81
LSLSLSL+H H S+ S + L+N + K S H+++ +AV
Sbjct: 200 LSLSLSLNHHHWRSNGSSVMNLSNRF--------GSNSKTAILNDSYSDENHSSSAAAAV 251
Query: 82 RITHIP 87
IP
Sbjct: 252 TSVVIP 257
>At3g27200 blue copper protein, putative
Length = 174
Score = 27.7 bits (60), Expect = 4.1
Identities = 22/73 (30%), Positives = 36/73 (49%), Gaps = 1/73 (1%)
Query: 9 SLSLSLSLSLSLSLSLSLSLSHTHTHSSSSGSVGLTNEIMKVDVQLKNEDLKVDTYRSG- 67
S L S++ S + + LS T T + G+VG + MK+ V + + D K + SG
Sbjct: 76 SCDLGTSVNSLSSGNDVVKLSKTGTRYFACGTVGHCEQGMKIKVNVVSSDSKSASSPSGS 135
Query: 68 GSGGQHANTTNSA 80
GSG + + S+
Sbjct: 136 GSGSDSGSGSGSS 148
>At4g30500 unknown protein
Length = 173
Score = 27.3 bits (59), Expect = 5.4
Identities = 13/35 (37%), Positives = 23/35 (65%), Gaps = 1/35 (2%)
Query: 95 QDERSQHMNKAKALKILCAKLYEMERLRL-HSNRS 128
+DE ++HM K + + ILC LY + R+ + HS+ +
Sbjct: 10 KDENARHMAKLRLIMILCNILYVLVRVIISHSSHT 44
>At1g79280 hypothetical protein
Length = 2111
Score = 27.3 bits (59), Expect = 5.4
Identities = 17/56 (30%), Positives = 32/56 (56%), Gaps = 3/56 (5%)
Query: 94 IQDERSQHMNKAKALKI-LCAKLYEMERLRLHSNRSKLRSEQIGSGDRSERIRTYN 148
++ E +++ K K ++ LC K EME+LR+ ++ K R +++ R+ I YN
Sbjct: 1325 MESENFENLLKTKQTELDLCMK--EMEKLRMETDLHKKRVDELRETYRNIDIADYN 1378
>At5g56210 unknown protein
Length = 509
Score = 26.9 bits (58), Expect = 7.0
Identities = 12/44 (27%), Positives = 25/44 (56%)
Query: 94 IQDERSQHMNKAKALKILCAKLYEMERLRLHSNRSKLRSEQIGS 137
++D+ S M +KALK + + L + ++H ++R + +GS
Sbjct: 55 VKDDTSVSMENSKALKRVLSGLVDPNAKQMHLPGPEVRQDSVGS 98
>At3g56600 putative protein
Length = 533
Score = 26.9 bits (58), Expect = 7.0
Identities = 17/44 (38%), Positives = 23/44 (51%), Gaps = 8/44 (18%)
Query: 148 NFPQGRVTDHRVGITYHSIDDVMQGENLDVFIDALLLREEMDAV 191
N+PQ V + + Y S NLD F DA LLR E+D++
Sbjct: 296 NWPQASVPFTDIELQYIS--------NLDPFKDAELLRTELDSI 331
>At5g27890 putative protein
Length = 799
Score = 26.6 bits (57), Expect = 9.1
Identities = 14/42 (33%), Positives = 21/42 (49%)
Query: 22 LSLSLSLSHTHTHSSSSGSVGLTNEIMKVDVQLKNEDLKVDT 63
+S SL+ T HS SS +G + + + V ED V+T
Sbjct: 347 ISASLAAVRTRDHSPSSSEMGKASRQIPIYVDTFGEDSSVET 388
>At4g36180 receptor protein kinase like protein
Length = 1136
Score = 26.6 bits (57), Expect = 9.1
Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
Query: 7 SLSLSLSLSLSLSLSLSLSLSLSHTHTHSSSSGSVGLTNEIMKVDVQLKN 56
+LS + +S S SL+ SLSL H H GS + + K+D+ + N
Sbjct: 631 NLSGEIPPEISQSSSLN-SLSLDHNHLSGVIPGSFSGLSNLTKMDLSVNN 679
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.314 0.128 0.339
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,799,921
Number of Sequences: 26719
Number of extensions: 147676
Number of successful extensions: 500
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 423
Number of HSP's gapped (non-prelim): 35
length of query: 197
length of database: 11,318,596
effective HSP length: 94
effective length of query: 103
effective length of database: 8,807,010
effective search space: 907122030
effective search space used: 907122030
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0143c.1