BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0346.9
(569 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BM813055 80 3e-15
TC87179 similar to PIR|T45627|T45627 replication factor A-like p... 51 1e-06
TC91093 weakly similar to PIR|T01873|T01873 hypothetical protein... 49 7e-06
BF646503 38 0.009
AJ501083 38 0.009
BF649736 35 0.10
TC82757 similar to PIR|T08563|T08563 dnaJ-related protein T22F8.... 34 0.13
BM815608 33 0.23
TC84341 homologue to GP|48188|emb|CAA77863.1|| N-terminus of bet... 33 0.39
TC90756 similar to PIR|T41394|T41394 hypothetical serine-rich pr... 31 1.1
TC82515 similar to GP|3688284|emb|CAA09694.1 lanatoside 15'-O-ac... 30 1.9
TC81458 similar to GP|21553367|gb|AAM62460.1 DnaJ protein-like {... 30 3.3
TC76980 similar to GP|14487954|gb|AAK63815.1 early light inducib... 29 5.6
TC80010 similar to GP|9758185|dbj|BAB08570.1 gene_id:MTE17.25~pi... 28 7.3
BQ150237 28 7.3
TC93131 similar to GP|19310729|gb|AAL85095.1 unknown protein {Ar... 28 9.5
>BM813055
Length = 549
Score = 79.7 bits (195), Expect = 3e-15
Identities = 44/142 (30%), Positives = 79/142 (54%), Gaps = 1/142 (0%)
Frame = +3
Query: 242 VVLGTMKYIPDGMDWYYPAC-KCSKKVYPADGMYFCEACNRHVASPLYKFRIQLRVMNSK 300
+VL T+ I WYY AC KC+ +V G +C CN + + +F++ ++VM++
Sbjct: 75 IVLATICGIESEYSWYYQACTKCAGRVRTVAGRLYCGKCNTG-RNAVPRFKLHVQVMDNT 251
Query: 301 NTATFVLFDQEANSVVKKPCAILVDASNKAGPDSPNVPSDILDLIDRTFLFKIEVSNSSN 360
+ +F+LFD+ +S V + ++DA +S + PSD+ + + LFK+EVS+ +
Sbjct: 252 GSTSFILFDRNVSSYVNRTVQDMIDAQPN---NSSDYPSDLDSFVGKRMLFKVEVSDGNL 422
Query: 361 SIYEPSYRVKKFCYDPEIINQF 382
+Y VK+ + ++INQF
Sbjct: 423 LHNWRNYAVKRTTAEEDVINQF 488
>TC87179 similar to PIR|T45627|T45627 replication factor A-like protein -
Arabidopsis thaliana, partial (84%)
Length = 2245
Score = 51.2 bits (121), Expect = 1e-06
Identities = 39/170 (22%), Positives = 75/170 (43%), Gaps = 16/170 (9%)
Frame = +2
Query: 182 ILFNPAIDEATKIRERNDPASQVLSQLQDSGMIYVEEDLLRQTDGKTIEQIKDLTEKSYC 241
+L NP + EA K+R D S+ +D+ M + + ++ + D SY
Sbjct: 1391 VLVNPEVPEAQKLRCWYD------SEGKDAAMAAISSGSISSSNSGSRSVYSDRVPLSYI 1552
Query: 242 V--------------VLGTMKYIPDGMDWYYPACK-CSKKVYP-ADGMYFCEACNRHVAS 285
+ G + +I +Y ACK C+KKV D Y+CE+C ++ A
Sbjct: 1553 TSNLSLGAEKPAFFSIRGNISFIKADQAMWYRACKTCNKKVTEDMDAGYWCESCQKNDAE 1732
Query: 286 PLYKFRIQLRVMNSKNTATFVLFDQEANSVVKKPCAILVDASNKAGPDSP 335
+++ + +V ++ A F++EA ++ P L + ++ G ++P
Sbjct: 1733 CNFRYIMVAKVSDASGEAFVSTFNEEAEKIIGCPAEELDNLKSQDGEENP 1882
>TC91093 weakly similar to PIR|T01873|T01873 hypothetical protein T24M8.10 -
Arabidopsis thaliana, partial (11%)
Length = 701
Score = 48.5 bits (114), Expect = 7e-06
Identities = 32/123 (26%), Positives = 59/123 (47%), Gaps = 3/123 (2%)
Frame = +3
Query: 18 WNIVAKVQRLWLSPSLYGSKLPFSLDMVLVDSKGDKIHATVRKTLIYRFQSLLTEGRVYQ 77
W I ++ +W+ ++ + L+MVL DSKGDKI + K I ++ S+L E + Y
Sbjct: 6 WMIGVRITDMWIVTNMNNHQ---HLEMVLTDSKGDKIQGIIVKDDIAKWGSILIEQKTYV 176
Query: 78 ISFFGVGESGRDFRTTGHAFKINFQIHTFVCVVTNTPINHSPYSFMAISEIM---FNDPD 134
+ F V ++ F+ H K+ + +C I + F +EI+ ++P+
Sbjct: 177 MQNFKVLKNDLQFKLCDHPCKMIIHGGSVICEHPFPNIPRTKVEFKTFAEILTGKMSEPN 356
Query: 135 ASF 137
S+
Sbjct: 357 DSY 365
>BF646503
Length = 640
Score = 38.1 bits (87), Expect = 0.009
Identities = 22/67 (32%), Positives = 34/67 (49%)
Frame = +1
Query: 140 DVIGILTGASGEHEFEKDGVSQKKITLELDQDGIHNVYGATKILFNPAIDEATKIRERND 199
DVIG+LTG E E+E++G + K + ++ DG Y LF +DE +
Sbjct: 256 DVIGMLTGVGTEREYERNGSATKLNVIAMEADG----YKLQCTLFGTYVDELNTFLATGE 423
Query: 200 PASQVLS 206
A+ V+S
Sbjct: 424 TANVVVS 444
>AJ501083
Length = 679
Score = 38.1 bits (87), Expect = 0.009
Identities = 15/41 (36%), Positives = 25/41 (60%)
Frame = -1
Query: 8 ISTINPSKETWNIVAKVQRLWLSPSLYGSKLPFSLDMVLVD 48
IS ++P K++W + +V R W KL FS+++VL+D
Sbjct: 598 ISHVSPRKQSWTLXVRVIRAWFVQDYKNKKLSFSMELVLMD 476
>BF649736
Length = 274
Score = 34.7 bits (78), Expect = 0.10
Identities = 18/54 (33%), Positives = 27/54 (49%)
Frame = +3
Query: 1 MSGKIYVISTINPSKETWNIVAKVQRLWLSPSLYGSKLPFSLDMVLVDSKGDKI 54
MS I + + P + WNI +V RLW S ++MVL+D G++I
Sbjct: 108 MSPPITSLLNVRPPRMAWNIRVRVVRLWXIMSSLVRGXVIFMEMVLLDQDGNRI 269
>TC82757 similar to PIR|T08563|T08563 dnaJ-related protein T22F8.50 -
Arabidopsis thaliana, partial (95%)
Length = 1317
Score = 34.3 bits (77), Expect = 0.13
Identities = 21/81 (25%), Positives = 39/81 (47%)
Frame = +2
Query: 487 PNAGKNKLKEKMDCLKTARDEKLKTLHAEIIEAVVAPNDSDDNASAGGEVSPQSKRPSPS 546
P K++EKM + R+EKLK++ + ++ V + + A E SK
Sbjct: 452 PQVRMQKIQEKMKVWQKEREEKLKSVLIDRLQPFVDGREEEFTTWANSEARNLSKAAFGE 631
Query: 547 AVDVKIGSVGSKKILKSVKKE 567
A+ IG + ++K K ++K+
Sbjct: 632 AMLHTIGYIYTRKAAKELEKD 694
>BM815608
Length = 558
Score = 33.5 bits (75), Expect = 0.23
Identities = 17/47 (36%), Positives = 28/47 (59%)
Frame = +3
Query: 338 PSDILDLIDRTFLFKIEVSNSSNSIYEPSYRVKKFCYDPEIINQFIS 384
P ++ +D+ LF++EVS+++ +Y VKK D EI QF+S
Sbjct: 201 PQELNMGVDKRMLFRVEVSDANLYRNWRTYAVKKMTEDEEITKQFLS 341
>TC84341 homologue to GP|48188|emb|CAA77863.1|| N-terminus of beta subunit
of RNA polymerase {Thermotoga maritima}, partial (3%)
Length = 687
Score = 32.7 bits (73), Expect = 0.39
Identities = 18/63 (28%), Positives = 32/63 (50%), Gaps = 2/63 (3%)
Frame = -2
Query: 364 EPSYRVKKFCYDPEIINQFISNSPHLMYDESNPFYFLATPTSVLKQESINPEISK--DLN 421
EP + FC P +I+ F +++ D +P +F +PTS ++ SI + +L
Sbjct: 686 EPRPKSSPFCQLPGVISDFNLTLENILXDLVSPLWFATSPTSPSEERSIKRSHVEHVELE 507
Query: 422 VEF 424
+EF
Sbjct: 506 IEF 498
>TC90756 similar to PIR|T41394|T41394 hypothetical serine-rich protein -
fission yeast (Schizosaccharomyces pombe), partial (7%)
Length = 1430
Score = 31.2 bits (69), Expect = 1.1
Identities = 13/27 (48%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
Frame = -1
Query: 256 WYYPAC-KCSKKVYPADGMYFCEACNR 281
WYY AC KC+ +V G FC CN+
Sbjct: 611 WYYEACTKCAGRVTIVAGKMFCGRCNQ 531
>TC82515 similar to GP|3688284|emb|CAA09694.1 lanatoside
15'-O-acetylesterase {Digitalis lanata}, partial (76%)
Length = 1230
Score = 30.4 bits (67), Expect = 1.9
Identities = 26/107 (24%), Positives = 49/107 (45%), Gaps = 4/107 (3%)
Frame = +1
Query: 182 ILFNPAIDEATKIRERNDPASQVLSQLQDSGMIYVEED--LLRQTDGKTIEQIKDLTEKS 239
I +N A+D+ K+ + + +Q L D+ +IYV+ + L+ T +K T+
Sbjct: 814 ITYNNAVDDYNKLLK--ETLTQTRKSLSDASLIYVDTNSALMELFRHPTSYGLKHSTKAC 987
Query: 240 YCVVLGTMKYIPDGM--DWYYPACKCSKKVYPADGMYFCEACNRHVA 284
G + P + + AC+ + DG++F EA N+ +A
Sbjct: 988 CGHGGGDYNFDPKALCGNMLASACEDPQNYVSWDGIHFTEAANKIIA 1128
>TC81458 similar to GP|21553367|gb|AAM62460.1 DnaJ protein-like {Arabidopsis
thaliana}, partial (38%)
Length = 1178
Score = 29.6 bits (65), Expect = 3.3
Identities = 10/26 (38%), Positives = 18/26 (68%)
Frame = -2
Query: 372 FCYDPEIINQFISNSPHLMYDESNPF 397
FC D EI+N F+ + P+++Y S+ +
Sbjct: 1165 FCIDAEIVN*FVHHDPNIIYQGSSTY 1088
>TC76980 similar to GP|14487954|gb|AAK63815.1 early light inducible protein
{Medicago sativa}, partial (87%)
Length = 833
Score = 28.9 bits (63), Expect = 5.6
Identities = 13/34 (38%), Positives = 17/34 (49%)
Frame = -3
Query: 32 SLYGSKLPFSLDMVLVDSKGDKIHATVRKTLIYR 65
S + +PF LD L S ATV TL+Y+
Sbjct: 573 SAFEDMIPFDLDSTLTPSNNGTKEATVNNTLVYK 472
>TC80010 similar to GP|9758185|dbj|BAB08570.1
gene_id:MTE17.25~pir||T07080~similar to unknown protein
{Arabidopsis thaliana}, partial (50%)
Length = 1610
Score = 28.5 bits (62), Expect = 7.3
Identities = 37/168 (22%), Positives = 69/168 (41%), Gaps = 7/168 (4%)
Frame = +3
Query: 408 KQESINPEISKDLNVEFAVAGELPNTDSPPASAVDLGCDTQNLSFDGGSSDMSGIKLIAI 467
K E NP+I +N + + E TDS + DT+NLS + G + + ++
Sbjct: 861 KIEFPNPKI---VNEDHLMVSEYFGTDSLATT------DTENLSSEKGVQLVESFSVCSV 1013
Query: 468 YGDSVGSDTSPP-KGGSVESPN---AGKNKLKEK---MDCLKTARDEKLKTLHAEIIEAV 520
SPP G V SP+ + K++ K + + + + + H E +
Sbjct: 1014 ESVQPPKVESPPSSNGVVSSPSVAASSKSRAATKPPNQEQISGTKKKNVDLKHGESDSSN 1193
Query: 521 VAPNDSDDNASAGGEVSPQSKRPSPSAVDVKIGSVGSKKILKSVKKEK 568
N+S + + P+ K VD+ + S+ ++ +S+ K K
Sbjct: 1194 TLLNESFPSPVVSVNIEPEPKMVQQDIVDMYLKSM--QQFTESLAKMK 1331
>BQ150237
Length = 668
Score = 28.5 bits (62), Expect = 7.3
Identities = 12/50 (24%), Positives = 27/50 (54%)
Frame = -2
Query: 272 GMYFCEACNRHVASPLYKFRIQLRVMNSKNTATFVLFDQEANSVVKKPCA 321
G++ C+A N + + +FR+ L + KN +T ++ ++ K+ C+
Sbjct: 604 GLFSCQALNGGLRLGVQRFRVLLALSTPKNLSTVMVQSSGQSACDKRSCS 455
>TC93131 similar to GP|19310729|gb|AAL85095.1 unknown protein {Arabidopsis
thaliana}, partial (22%)
Length = 786
Score = 28.1 bits (61), Expect = 9.5
Identities = 12/41 (29%), Positives = 17/41 (41%)
Frame = +1
Query: 239 SYCVVLGTMKYIPDGMDWYYPACKCSKKVYPADGMYFCEAC 279
S C + DG +W+ P C C ++ G F E C
Sbjct: 154 SLCCFCDSFFRASDGFNWFSPECTCGNRI---AGFMFSENC 267
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.134 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,195,965
Number of Sequences: 36976
Number of extensions: 218296
Number of successful extensions: 1113
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1111
length of query: 569
length of database: 9,014,727
effective HSP length: 101
effective length of query: 468
effective length of database: 5,280,151
effective search space: 2471110668
effective search space used: 2471110668
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0346.9