
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0309.4
(116 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC85352 weakly similar to GP|9279599|dbj|BAB01057.1 contains sim... 213 1e-56
BQ144095 weakly similar to GP|12842949|dbj| data source:SPTR so... 42 3e-05
TC81712 similar to GP|23617251|dbj|BAC20918. putative transcript... 42 5e-05
TC80108 similar to GP|22045251|gb|AAL69483.2 putative SPL1-relat... 30 0.14
TC84595 weakly similar to SP|Q9M7J4|MFP1_TOBAC MAR binding filam... 29 0.30
TC84683 similar to GP|13878013|gb|AAK44084.1 putative zinc finge... 27 0.88
TC87607 similar to SP|Q9ZTS1|SYM_ORYSA Probable methionyl-tRNA s... 27 1.5
BF645697 similar to GP|12659318|gb KEULE {Arabidopsis thaliana},... 25 3.4
TC79650 similar to GP|20197545|gb|AAD12706.2 expressed protein {... 25 5.7
BQ144035 similar to GP|21732311|emb hypothetical protein {Homo s... 25 5.7
TC85547 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2... 24 7.5
TC89592 similar to GP|20197026|gb|AAM14882.1 expressed protein {... 24 7.5
BQ123697 PIR|T47839|T47 hypothetical protein T2O9.130 - Arabidop... 24 7.5
TC85548 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2... 24 7.5
TC81849 similar to GP|19698885|gb|AAL91178.1 putative protein {A... 24 9.8
BQ139229 weakly similar to GP|20453199|gb AT3g18290/MIE15_8 {Ara... 24 9.8
TC85784 similar to GP|15131688|emb|CAC48323. 2-Cys peroxiredoxin... 24 9.8
TC82570 similar to PIR|T51853|T51853 RING-H2 finger protein RHF1... 24 9.8
BQ137344 24 9.8
TC93354 weakly similar to GP|13561914|gb|AAK30565.1 coat protein... 24 9.8
>TC85352 weakly similar to GP|9279599|dbj|BAB01057.1 contains similarity to
transcription-associated zinc ribbon
protein~gene_id:MPE11.11, partial (88%)
Length = 899
Score = 213 bits (541), Expect = 1e-56
Identities = 99/114 (86%), Positives = 105/114 (91%)
Frame = +1
Query: 3 YSRERDFLFCHYCGTMLTVPSVNYAECPLCKNRSNIKDIEGKEISYTISAEEIRRELGIE 62
YSR RDFLFCH CGTMLTVPS +YA CPLCK + NIKDI+GKEISYTISAE+IRRELGI+
Sbjct: 211 YSRGRDFLFCHLCGTMLTVPSTDYALCPLCKTKCNIKDIKGKEISYTISAEDIRRELGID 390
Query: 63 TIEEQKVQLSKVNKTCEKCGNGEATFYTRQMRSADEGQTTFYACTRCGHQFQEN 116
IEEQKVQLSKVNKTCEKCG+GEA FYTRQMRSADEGQTTFY CTRCGHQFQEN
Sbjct: 391 LIEEQKVQLSKVNKTCEKCGHGEAAFYTRQMRSADEGQTTFYTCTRCGHQFQEN 552
>BQ144095 weakly similar to GP|12842949|dbj| data source:SPTR source
key:Q9Y2Y1 evidence:ISS~homolog to PUTATIVE
DNA-DIRECTED RNA POLYMERASE, partial (39%)
Length = 615
Score = 42.4 bits (98), Expect = 3e-05
Identities = 18/45 (40%), Positives = 29/45 (64%), Gaps = 2/45 (4%)
Frame = +2
Query: 73 KVNKTCEKCGNGEATFYTRQMRSADEGQTTFYAC--TRCGHQFQE 115
K + C C + +A+++ +Q RSADE T FY C +CGH+++E
Sbjct: 311 KADVPCPNCRHPQASYHMQQTRSADEPATIFYECLNEKCGHKWKE 445
>TC81712 similar to GP|23617251|dbj|BAC20918. putative transcription
elongation factor {Oryza sativa (japonica
cultivar-group)}, partial (34%)
Length = 721
Score = 41.6 bits (96), Expect = 5e-05
Identities = 15/37 (40%), Positives = 23/37 (61%)
Frame = +1
Query: 78 CEKCGNGEATFYTRQMRSADEGQTTFYACTRCGHQFQ 114
C +CG + T+Y Q RSADE TT+ C C ++++
Sbjct: 412 CGRCGQRKTTYYQMQTRSADEPMTTYVTCVNCNNRWK 522
>TC80108 similar to GP|22045251|gb|AAL69483.2 putative SPL1-related protein
{Arabidopsis thaliana}, partial (42%)
Length = 1251
Score = 30.0 bits (66), Expect = 0.14
Identities = 15/46 (32%), Positives = 24/46 (51%)
Frame = +2
Query: 40 DIEGKEISYTISAEEIRRELGIETIEEQKVQLSKVNKTCEKCGNGE 85
D + E+S I E LGIE ++++ Q+ +V +C KC E
Sbjct: 365 DRQRSEVSVRIDNEIEHPSLGIELMQKRINQVKRVGDSCSKCAIAE 502
>TC84595 weakly similar to SP|Q9M7J4|MFP1_TOBAC MAR binding filament-like
protein 1-1. [Common tobacco] {Nicotiana tabacum},
partial (10%)
Length = 710
Score = 28.9 bits (63), Expect = 0.30
Identities = 22/84 (26%), Positives = 33/84 (39%)
Frame = +1
Query: 32 CKNRSNIKDIEGKEISYTISAEEIRRELGIETIEEQKVQLSKVNKTCEKCGNGEATFYTR 91
C NR+N + EGK + EE+R E+ E+ G T +
Sbjct: 487 CNNRNNEQQTEGKRRAVRFIVEELRVEV------------------IERAGGTSQTTWKG 612
Query: 92 QMRSADEGQTTFYACTRCGHQFQE 115
+ R+ +TT + C HQF E
Sbjct: 613 KGRAXSSDRTTKF-CKEYCHQFGE 681
>TC84683 similar to GP|13878013|gb|AAK44084.1 putative zinc finger protein
{Arabidopsis thaliana}, partial (9%)
Length = 544
Score = 27.3 bits (59), Expect = 0.88
Identities = 11/29 (37%), Positives = 14/29 (47%), Gaps = 4/29 (13%)
Frame = +2
Query: 12 CHYCGTMLTVPSVNYAECPL----CKNRS 36
CHYCG V + CP+ C+N S
Sbjct: 77 CHYCGIGCKVQEHGFGHCPIKAANCENES 163
>TC87607 similar to SP|Q9ZTS1|SYM_ORYSA Probable methionyl-tRNA synthetase
(EC 6.1.1.10) (Methionine--tRNA ligase) (MetRS). [Rice],
partial (54%)
Length = 1726
Score = 26.6 bits (57), Expect = 1.5
Identities = 9/31 (29%), Positives = 15/31 (48%)
Frame = +2
Query: 12 CHYCGTMLTVPSVNYAECPLCKNRSNIKDIE 42
C CG +L + +C +C+N I D +
Sbjct: 677 CENCGNLLNPTELKIPKCKVCRNSPRICDTD 769
>BF645697 similar to GP|12659318|gb KEULE {Arabidopsis thaliana}, partial
(21%)
Length = 643
Score = 25.4 bits (54), Expect = 3.4
Identities = 9/16 (56%), Positives = 10/16 (62%)
Frame = +3
Query: 4 SRERDFLFCHYCGTML 19
S DFLFC YC +L
Sbjct: 330 SNTSDFLFCSYCSALL 377
>TC79650 similar to GP|20197545|gb|AAD12706.2 expressed protein {Arabidopsis
thaliana}, partial (62%)
Length = 1439
Score = 24.6 bits (52), Expect = 5.7
Identities = 21/53 (39%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Frame = +3
Query: 41 IEGKEISYTISAEEIRR--ELGIETIEEQKVQLSKVNKTCEKCGNGEATFYTR 91
IE + Y SAEE+RR EL + IEE ++ + K K EK YTR
Sbjct: 951 IELPDSFYKRSAEEVRREAELRRKKIEESQLLIPKSLK--EKQAKAAKRRYTR 1103
Score = 23.9 bits (50), Expect = 9.8
Identities = 15/60 (25%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = +1
Query: 23 SVNYAECPLCKNRSNIKDIEGKEISYTISAEEIRRELGIETIEEQK-VQLSKVNKTCEKC 81
S+N ECP+CK +D + + +S ++ + + + K Q+S+ N E C
Sbjct: 346 SLNVYECPICKQPFRSEDEVTEHVESCLSNPIENDDVDEKCVSKLKESQVSESNTELEIC 525
>BQ144035 similar to GP|21732311|emb hypothetical protein {Homo sapiens},
partial (4%)
Length = 1340
Score = 24.6 bits (52), Expect = 5.7
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = -1
Query: 9 FLFCHYCGTMLTVPSVNYAEC 29
F+FC Y GT L V + + +C
Sbjct: 236 FVFCGYAGTGLMVTTGRHGQC 174
>TC85547 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2a
[imported] - Arabidopsis thaliana, partial (42%)
Length = 677
Score = 24.3 bits (51), Expect = 7.5
Identities = 8/27 (29%), Positives = 16/27 (58%)
Frame = +2
Query: 27 AECPLCKNRSNIKDIEGKEISYTISAE 53
++CP+C ++KD G+E+ + E
Sbjct: 302 SQCPMCWQSISLKDPTGQELLEAVERE 382
>TC89592 similar to GP|20197026|gb|AAM14882.1 expressed protein {Arabidopsis
thaliana}, partial (82%)
Length = 796
Score = 24.3 bits (51), Expect = 7.5
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -2
Query: 69 VQLSKVNKTCEKCGNGEATFYTRQMRS 95
VQ S N +KCG+ +ATF+ + + S
Sbjct: 549 VQAS*SNSVWKKCGSHDATFFIQYVVS 469
>BQ123697 PIR|T47839|T47 hypothetical protein T2O9.130 - Arabidopsis
thaliana, partial (16%)
Length = 618
Score = 24.3 bits (51), Expect = 7.5
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -1
Query: 69 VQLSKVNKTCEKCGNGEATFYTRQMRS 95
VQ S N +KCG+ +ATF+ + + S
Sbjct: 534 VQAS*SNSVWKKCGSHDATFFIQYVVS 454
>TC85548 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2a
[imported] - Arabidopsis thaliana, partial (69%)
Length = 1554
Score = 24.3 bits (51), Expect = 7.5
Identities = 8/27 (29%), Positives = 16/27 (58%)
Frame = +3
Query: 27 AECPLCKNRSNIKDIEGKEISYTISAE 53
++CP+C ++KD G+E+ + E
Sbjct: 303 SQCPMCWQSISLKDPTGQELLEAVERE 383
>TC81849 similar to GP|19698885|gb|AAL91178.1 putative protein {Arabidopsis
thaliana}, partial (16%)
Length = 1477
Score = 23.9 bits (50), Expect = 9.8
Identities = 10/24 (41%), Positives = 12/24 (49%)
Frame = +1
Query: 10 LFCHYCGTMLTVPSVNYAECPLCK 33
LFC C L+ ECP+CK
Sbjct: 214 LFCWPCFYQLSYAYSKAKECPVCK 285
>BQ139229 weakly similar to GP|20453199|gb AT3g18290/MIE15_8 {Arabidopsis
thaliana}, partial (4%)
Length = 648
Score = 23.9 bits (50), Expect = 9.8
Identities = 12/39 (30%), Positives = 16/39 (40%), Gaps = 1/39 (2%)
Frame = +1
Query: 73 KVNKTCEKCGNGEATFYTRQMRSAD-EGQTTFYACTRCG 110
K + TC CG A +Y + D + Y C CG
Sbjct: 187 KASATCHNCGIEAACYYCDICKLWDNNARKKIYHCPDCG 303
>TC85784 similar to GP|15131688|emb|CAC48323. 2-Cys peroxiredoxin {Pisum
sativum}, partial (86%)
Length = 1114
Score = 23.9 bits (50), Expect = 9.8
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = -2
Query: 34 NRSNIKDIEGKEISYTISAEEIRRELGIETIEEQ 67
+ +K+ EG+E + RELGIE +EE+
Sbjct: 300 SEGRVKESEGRE-------NGLGRELGIERVEEE 220
>TC82570 similar to PIR|T51853|T51853 RING-H2 finger protein RHF1a
[imported] - Arabidopsis thaliana (fragment), partial
(23%)
Length = 738
Score = 23.9 bits (50), Expect = 9.8
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +2
Query: 28 ECPLCKNRSNIKDIEGKEISYTISAEEIRR 57
ECP+C ++KD +E+ + AE+ R
Sbjct: 320 ECPICWQSLSLKDPACQELLDAVEAEKSLR 409
>BQ137344
Length = 933
Score = 23.9 bits (50), Expect = 9.8
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -3
Query: 3 YSRERDFLFCHYCGTMLTVPSVNY 26
Y+ + D L+CH TM +P++NY
Sbjct: 529 YNSKTDVLYCH---TM*RLPAMNY 467
>TC93354 weakly similar to GP|13561914|gb|AAK30565.1 coat protein {apple
stem pitting virus} [Apple stem pitting virus], partial
(5%)
Length = 933
Score = 23.9 bits (50), Expect = 9.8
Identities = 11/32 (34%), Positives = 17/32 (52%)
Frame = -1
Query: 69 VQLSKVNKTCEKCGNGEATFYTRQMRSADEGQ 100
VQ+ K++ CE CG+ E R +R D +
Sbjct: 825 VQVHKLHGRCESCGHAE-----RHLRHLDRAE 745
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.133 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,989,677
Number of Sequences: 36976
Number of extensions: 43974
Number of successful extensions: 289
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 287
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 289
length of query: 116
length of database: 9,014,727
effective HSP length: 92
effective length of query: 24
effective length of database: 5,612,935
effective search space: 134710440
effective search space used: 134710440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)
Lotus: description of TM0309.4