Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0309.4
         (116 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC85352 weakly similar to GP|9279599|dbj|BAB01057.1 contains sim...   213  1e-56
BQ144095 weakly similar to GP|12842949|dbj| data source:SPTR  so...    42  3e-05
TC81712 similar to GP|23617251|dbj|BAC20918. putative transcript...    42  5e-05
TC80108 similar to GP|22045251|gb|AAL69483.2 putative SPL1-relat...    30  0.14
TC84595 weakly similar to SP|Q9M7J4|MFP1_TOBAC MAR binding filam...    29  0.30
TC84683 similar to GP|13878013|gb|AAK44084.1 putative zinc finge...    27  0.88
TC87607 similar to SP|Q9ZTS1|SYM_ORYSA Probable methionyl-tRNA s...    27  1.5
BF645697 similar to GP|12659318|gb KEULE {Arabidopsis thaliana},...    25  3.4
TC79650 similar to GP|20197545|gb|AAD12706.2 expressed protein {...    25  5.7
BQ144035 similar to GP|21732311|emb hypothetical protein {Homo s...    25  5.7
TC85547 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2...    24  7.5
TC89592 similar to GP|20197026|gb|AAM14882.1 expressed protein {...    24  7.5
BQ123697 PIR|T47839|T47 hypothetical protein T2O9.130 - Arabidop...    24  7.5
TC85548 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2...    24  7.5
TC81849 similar to GP|19698885|gb|AAL91178.1 putative protein {A...    24  9.8
BQ139229 weakly similar to GP|20453199|gb AT3g18290/MIE15_8 {Ara...    24  9.8
TC85784 similar to GP|15131688|emb|CAC48323. 2-Cys peroxiredoxin...    24  9.8
TC82570 similar to PIR|T51853|T51853 RING-H2 finger protein RHF1...    24  9.8
BQ137344                                                               24  9.8
TC93354 weakly similar to GP|13561914|gb|AAK30565.1 coat protein...    24  9.8

>TC85352 weakly similar to GP|9279599|dbj|BAB01057.1 contains similarity to
           transcription-associated zinc ribbon
           protein~gene_id:MPE11.11, partial (88%)
          Length = 899

 Score =  213 bits (541), Expect = 1e-56
 Identities = 99/114 (86%), Positives = 105/114 (91%)
 Frame = +1

Query: 3   YSRERDFLFCHYCGTMLTVPSVNYAECPLCKNRSNIKDIEGKEISYTISAEEIRRELGIE 62
           YSR RDFLFCH CGTMLTVPS +YA CPLCK + NIKDI+GKEISYTISAE+IRRELGI+
Sbjct: 211 YSRGRDFLFCHLCGTMLTVPSTDYALCPLCKTKCNIKDIKGKEISYTISAEDIRRELGID 390

Query: 63  TIEEQKVQLSKVNKTCEKCGNGEATFYTRQMRSADEGQTTFYACTRCGHQFQEN 116
            IEEQKVQLSKVNKTCEKCG+GEA FYTRQMRSADEGQTTFY CTRCGHQFQEN
Sbjct: 391 LIEEQKVQLSKVNKTCEKCGHGEAAFYTRQMRSADEGQTTFYTCTRCGHQFQEN 552


>BQ144095 weakly similar to GP|12842949|dbj| data source:SPTR  source
           key:Q9Y2Y1  evidence:ISS~homolog to PUTATIVE
           DNA-DIRECTED RNA POLYMERASE, partial (39%)
          Length = 615

 Score = 42.4 bits (98), Expect = 3e-05
 Identities = 18/45 (40%), Positives = 29/45 (64%), Gaps = 2/45 (4%)
 Frame = +2

Query: 73  KVNKTCEKCGNGEATFYTRQMRSADEGQTTFYAC--TRCGHQFQE 115
           K +  C  C + +A+++ +Q RSADE  T FY C   +CGH+++E
Sbjct: 311 KADVPCPNCRHPQASYHMQQTRSADEPATIFYECLNEKCGHKWKE 445


>TC81712 similar to GP|23617251|dbj|BAC20918. putative transcription
           elongation factor {Oryza sativa (japonica
           cultivar-group)}, partial (34%)
          Length = 721

 Score = 41.6 bits (96), Expect = 5e-05
 Identities = 15/37 (40%), Positives = 23/37 (61%)
 Frame = +1

Query: 78  CEKCGNGEATFYTRQMRSADEGQTTFYACTRCGHQFQ 114
           C +CG  + T+Y  Q RSADE  TT+  C  C ++++
Sbjct: 412 CGRCGQRKTTYYQMQTRSADEPMTTYVTCVNCNNRWK 522


>TC80108 similar to GP|22045251|gb|AAL69483.2 putative SPL1-related protein
           {Arabidopsis thaliana}, partial (42%)
          Length = 1251

 Score = 30.0 bits (66), Expect = 0.14
 Identities = 15/46 (32%), Positives = 24/46 (51%)
 Frame = +2

Query: 40  DIEGKEISYTISAEEIRRELGIETIEEQKVQLSKVNKTCEKCGNGE 85
           D +  E+S  I  E     LGIE ++++  Q+ +V  +C KC   E
Sbjct: 365 DRQRSEVSVRIDNEIEHPSLGIELMQKRINQVKRVGDSCSKCAIAE 502


>TC84595 weakly similar to SP|Q9M7J4|MFP1_TOBAC MAR binding filament-like
           protein 1-1. [Common tobacco] {Nicotiana tabacum},
           partial (10%)
          Length = 710

 Score = 28.9 bits (63), Expect = 0.30
 Identities = 22/84 (26%), Positives = 33/84 (39%)
 Frame = +1

Query: 32  CKNRSNIKDIEGKEISYTISAEEIRRELGIETIEEQKVQLSKVNKTCEKCGNGEATFYTR 91
           C NR+N +  EGK  +     EE+R E+                   E+ G    T +  
Sbjct: 487 CNNRNNEQQTEGKRRAVRFIVEELRVEV------------------IERAGGTSQTTWKG 612

Query: 92  QMRSADEGQTTFYACTRCGHQFQE 115
           + R+    +TT + C    HQF E
Sbjct: 613 KGRAXSSDRTTKF-CKEYCHQFGE 681


>TC84683 similar to GP|13878013|gb|AAK44084.1 putative zinc finger protein
           {Arabidopsis thaliana}, partial (9%)
          Length = 544

 Score = 27.3 bits (59), Expect = 0.88
 Identities = 11/29 (37%), Positives = 14/29 (47%), Gaps = 4/29 (13%)
 Frame = +2

Query: 12  CHYCGTMLTVPSVNYAECPL----CKNRS 36
           CHYCG    V    +  CP+    C+N S
Sbjct: 77  CHYCGIGCKVQEHGFGHCPIKAANCENES 163


>TC87607 similar to SP|Q9ZTS1|SYM_ORYSA Probable methionyl-tRNA synthetase
           (EC 6.1.1.10) (Methionine--tRNA ligase) (MetRS). [Rice],
           partial (54%)
          Length = 1726

 Score = 26.6 bits (57), Expect = 1.5
 Identities = 9/31 (29%), Positives = 15/31 (48%)
 Frame = +2

Query: 12  CHYCGTMLTVPSVNYAECPLCKNRSNIKDIE 42
           C  CG +L    +   +C +C+N   I D +
Sbjct: 677 CENCGNLLNPTELKIPKCKVCRNSPRICDTD 769


>BF645697 similar to GP|12659318|gb KEULE {Arabidopsis thaliana}, partial
           (21%)
          Length = 643

 Score = 25.4 bits (54), Expect = 3.4
 Identities = 9/16 (56%), Positives = 10/16 (62%)
 Frame = +3

Query: 4   SRERDFLFCHYCGTML 19
           S   DFLFC YC  +L
Sbjct: 330 SNTSDFLFCSYCSALL 377


>TC79650 similar to GP|20197545|gb|AAD12706.2 expressed protein {Arabidopsis
            thaliana}, partial (62%)
          Length = 1439

 Score = 24.6 bits (52), Expect = 5.7
 Identities = 21/53 (39%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
 Frame = +3

Query: 41   IEGKEISYTISAEEIRR--ELGIETIEEQKVQLSKVNKTCEKCGNGEATFYTR 91
            IE  +  Y  SAEE+RR  EL  + IEE ++ + K  K  EK        YTR
Sbjct: 951  IELPDSFYKRSAEEVRREAELRRKKIEESQLLIPKSLK--EKQAKAAKRRYTR 1103



 Score = 23.9 bits (50), Expect = 9.8
 Identities = 15/60 (25%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
 Frame = +1

Query: 23  SVNYAECPLCKNRSNIKDIEGKEISYTISAEEIRRELGIETIEEQK-VQLSKVNKTCEKC 81
           S+N  ECP+CK     +D   + +   +S      ++  + + + K  Q+S+ N   E C
Sbjct: 346 SLNVYECPICKQPFRSEDEVTEHVESCLSNPIENDDVDEKCVSKLKESQVSESNTELEIC 525


>BQ144035 similar to GP|21732311|emb hypothetical protein {Homo sapiens},
           partial (4%)
          Length = 1340

 Score = 24.6 bits (52), Expect = 5.7
 Identities = 9/21 (42%), Positives = 13/21 (61%)
 Frame = -1

Query: 9   FLFCHYCGTMLTVPSVNYAEC 29
           F+FC Y GT L V +  + +C
Sbjct: 236 FVFCGYAGTGLMVTTGRHGQC 174


>TC85547 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2a
           [imported] - Arabidopsis thaliana, partial (42%)
          Length = 677

 Score = 24.3 bits (51), Expect = 7.5
 Identities = 8/27 (29%), Positives = 16/27 (58%)
 Frame = +2

Query: 27  AECPLCKNRSNIKDIEGKEISYTISAE 53
           ++CP+C    ++KD  G+E+   +  E
Sbjct: 302 SQCPMCWQSISLKDPTGQELLEAVERE 382


>TC89592 similar to GP|20197026|gb|AAM14882.1 expressed protein {Arabidopsis
           thaliana}, partial (82%)
          Length = 796

 Score = 24.3 bits (51), Expect = 7.5
 Identities = 11/27 (40%), Positives = 17/27 (62%)
 Frame = -2

Query: 69  VQLSKVNKTCEKCGNGEATFYTRQMRS 95
           VQ S  N   +KCG+ +ATF+ + + S
Sbjct: 549 VQAS*SNSVWKKCGSHDATFFIQYVVS 469


>BQ123697 PIR|T47839|T47 hypothetical protein T2O9.130 - Arabidopsis
           thaliana, partial (16%)
          Length = 618

 Score = 24.3 bits (51), Expect = 7.5
 Identities = 11/27 (40%), Positives = 17/27 (62%)
 Frame = -1

Query: 69  VQLSKVNKTCEKCGNGEATFYTRQMRS 95
           VQ S  N   +KCG+ +ATF+ + + S
Sbjct: 534 VQAS*SNSVWKKCGSHDATFFIQYVVS 454


>TC85548 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2a
           [imported] - Arabidopsis thaliana, partial (69%)
          Length = 1554

 Score = 24.3 bits (51), Expect = 7.5
 Identities = 8/27 (29%), Positives = 16/27 (58%)
 Frame = +3

Query: 27  AECPLCKNRSNIKDIEGKEISYTISAE 53
           ++CP+C    ++KD  G+E+   +  E
Sbjct: 303 SQCPMCWQSISLKDPTGQELLEAVERE 383


>TC81849 similar to GP|19698885|gb|AAL91178.1 putative protein {Arabidopsis
           thaliana}, partial (16%)
          Length = 1477

 Score = 23.9 bits (50), Expect = 9.8
 Identities = 10/24 (41%), Positives = 12/24 (49%)
 Frame = +1

Query: 10  LFCHYCGTMLTVPSVNYAECPLCK 33
           LFC  C   L+       ECP+CK
Sbjct: 214 LFCWPCFYQLSYAYSKAKECPVCK 285


>BQ139229 weakly similar to GP|20453199|gb AT3g18290/MIE15_8 {Arabidopsis
           thaliana}, partial (4%)
          Length = 648

 Score = 23.9 bits (50), Expect = 9.8
 Identities = 12/39 (30%), Positives = 16/39 (40%), Gaps = 1/39 (2%)
 Frame = +1

Query: 73  KVNKTCEKCGNGEATFYTRQMRSAD-EGQTTFYACTRCG 110
           K + TC  CG   A +Y    +  D   +   Y C  CG
Sbjct: 187 KASATCHNCGIEAACYYCDICKLWDNNARKKIYHCPDCG 303


>TC85784 similar to GP|15131688|emb|CAC48323. 2-Cys peroxiredoxin {Pisum
           sativum}, partial (86%)
          Length = 1114

 Score = 23.9 bits (50), Expect = 9.8
 Identities = 12/34 (35%), Positives = 19/34 (55%)
 Frame = -2

Query: 34  NRSNIKDIEGKEISYTISAEEIRRELGIETIEEQ 67
           +   +K+ EG+E         + RELGIE +EE+
Sbjct: 300 SEGRVKESEGRE-------NGLGRELGIERVEEE 220


>TC82570 similar to PIR|T51853|T51853 RING-H2 finger protein RHF1a
           [imported] - Arabidopsis thaliana (fragment), partial
           (23%)
          Length = 738

 Score = 23.9 bits (50), Expect = 9.8
 Identities = 10/30 (33%), Positives = 17/30 (56%)
 Frame = +2

Query: 28  ECPLCKNRSNIKDIEGKEISYTISAEEIRR 57
           ECP+C    ++KD   +E+   + AE+  R
Sbjct: 320 ECPICWQSLSLKDPACQELLDAVEAEKSLR 409


>BQ137344 
          Length = 933

 Score = 23.9 bits (50), Expect = 9.8
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = -3

Query: 3   YSRERDFLFCHYCGTMLTVPSVNY 26
           Y+ + D L+CH   TM  +P++NY
Sbjct: 529 YNSKTDVLYCH---TM*RLPAMNY 467


>TC93354 weakly similar to GP|13561914|gb|AAK30565.1 coat protein {apple
           stem pitting virus} [Apple stem pitting virus], partial
           (5%)
          Length = 933

 Score = 23.9 bits (50), Expect = 9.8
 Identities = 11/32 (34%), Positives = 17/32 (52%)
 Frame = -1

Query: 69  VQLSKVNKTCEKCGNGEATFYTRQMRSADEGQ 100
           VQ+ K++  CE CG+ E     R +R  D  +
Sbjct: 825 VQVHKLHGRCESCGHAE-----RHLRHLDRAE 745


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.319    0.133    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,989,677
Number of Sequences: 36976
Number of extensions: 43974
Number of successful extensions: 289
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 287
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 289
length of query: 116
length of database: 9,014,727
effective HSP length: 92
effective length of query: 24
effective length of database: 5,612,935
effective search space: 134710440
effective search space used: 134710440
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)


Lotus: description of TM0309.4