BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0083.3
(287 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG585866 74 8e-14
BF640104 38 0.004
BQ148771 36 0.015
TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarot... 35 0.025
TC82948 33 0.13
AW694688 similar to PIR|T00246|T002 DNA polymerase V - fission y... 32 0.28
BG585933 similar to GP|452446|gb|A cycloartenol synthase; (S)-2 ... 32 0.37
AJ497561 weakly similar to GP|10140689|gb putative non-LTR retro... 31 0.48
BQ144198 29 2.4
BG586720 similar to GP|19070477|gb PTH-2 {Cucumis melo}, partial... 29 2.4
TC81272 similar to PIR|T05225|T05225 extensin homolog F17I5.160 ... 28 3.1
TC80388 28 3.1
BF633993 homologue to GP|22135972|g putative WD-40 repeat protei... 28 5.3
AI974304 similar to GP|12325083|gb| hypothetical protein; 3218-1... 28 5.3
AL378562 27 6.9
BI263281 weakly similar to GP|498038|gb|A lipid transfer protein... 27 6.9
TC78696 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17 ... 27 6.9
TC88933 weakly similar to GP|17381142|gb|AAL36383.1 unknown prot... 27 9.1
AW774658 similar to GP|2808681|emb| Hcr9-4B {Lycopersicon hirsut... 27 9.1
>BG585866
Length = 828
Score = 73.6 bits (179), Expect = 8e-14
Identities = 36/114 (31%), Positives = 58/114 (50%), Gaps = 1/114 (0%)
Frame = +3
Query: 138 LVKAFSVLRDGLSFAIHDGCTSFWYSDWTGLGKLCALVPFVHISDTALTISDVWVEGSWN 197
+++ +VL+ G ++ G +SFWY++W+ LG L PFV I D LT+ DV+ G +
Sbjct: 84 IIRDKNVLKSGYTWRAGSGNSSFWYTNWSSLGLLGTQAPFVDIHDLHLTVKDVFTTGGQH 263
Query: 198 LSMLYTELPDPYKPTITSMQGPSHETVDDCWVWNSNPAG-YSCSDAYQWLRDWT 250
LYT LP I + + ++ D ++W N G Y+ Y W+ T
Sbjct: 264 TQSLYTILPTDIAEVINNTHLNFNASIGDAYIWPHNSNGVYTAKSGYSWILSQT 425
>BF640104
Length = 344
Score = 38.1 bits (87), Expect = 0.004
Identities = 23/74 (31%), Positives = 41/74 (55%), Gaps = 1/74 (1%)
Frame = -1
Query: 2 NGEILATTTSVMGPVLTPVLSEALCLRWAIGMARDLGFRHVWFDTDCLVLFDYWRRSDEG 61
NG ++A+ T + P+ +EA + A A D GF++V F+ D L R +EG
Sbjct: 242 NGIVMASGTWNRPGFMCPITAEAWGVYQAALFALDQGFQNVLFENDNEKLISMLSREEEG 63
Query: 62 -RSHLGTVVRDCVN 74
RS+LG++++ ++
Sbjct: 62 HRSYLGSIIQSIIS 21
>BQ148771
Length = 680
Score = 36.2 bits (82), Expect = 0.015
Identities = 27/87 (31%), Positives = 40/87 (45%), Gaps = 10/87 (11%)
Frame = -3
Query: 73 VNWKQIVLPKNQGGLGVRSTRESNVSLLGKLAWKL--GSRIFITKYLKG--------SSI 122
V W+ + PK GLG+R N + + KL W + GS T+ ++G I
Sbjct: 384 VGWETMSKPKTIYGLGLRRLDVMNKACIMKLGWSIYSGSNSLCTEVMRGKYQRSESLEEI 205
Query: 123 FSYELTGSSSYVWKGLVKAFSVLRDGL 149
F + T SS +WK LVK + + L
Sbjct: 204 FLEKPTDSS--LWKALVKLWPEIERNL 130
>TC77455 similar to GP|22335695|dbj|BAC10549. nine-cis-epoxycarotenoid
dioxygenase1 {Pisum sativum}, partial (43%)
Length = 1865
Score = 35.4 bits (80), Expect = 0.025
Identities = 17/28 (60%), Positives = 20/28 (70%)
Frame = -3
Query: 80 LPKNQGGLGVRSTRESNVSLLGKLAWKL 107
LP+ +GGLGVR R NVSLL K W+L
Sbjct: 966 LPRCKGGLGVRDIRLVNVSLLAKWWWRL 883
>TC82948
Length = 705
Score = 33.1 bits (74), Expect = 0.13
Identities = 16/42 (38%), Positives = 26/42 (61%)
Frame = +3
Query: 66 GTVVRDCVNWKQIVLPKNQGGLGVRSTRESNVSLLGKLAWKL 107
G VV+ V+W+++ P +G LG+RS + N +L KL W +
Sbjct: 252 GKVVK--VSWEKVCRPIKEGSLGIRSLSKLNEALNLKLCWDM 371
>AW694688 similar to PIR|T00246|T002 DNA polymerase V - fission yeast
(Schizosaccharomyces pombe), partial (1%)
Length = 605
Score = 32.0 bits (71), Expect = 0.28
Identities = 18/55 (32%), Positives = 26/55 (46%)
Frame = -1
Query: 3 GEILATTTSVMGPVLTPVLSEALCLRWAIGMARDLGFRHVWFDTDCLVLFDYWRR 57
G +A S V+ EA+ L I +GF+H F+TDC +L D + R
Sbjct: 170 GNFVAAFASWTDAVMQVKEGEAVVLLGVIKWVNIMGFQHANFETDCKLLIDTYPR 6
>BG585933 similar to GP|452446|gb|A cycloartenol synthase; (S)-2
3-epoxysqualene mutase {Arabidopsis thaliana}, partial
(24%)
Length = 858
Score = 31.6 bits (70), Expect = 0.37
Identities = 16/44 (36%), Positives = 23/44 (51%), Gaps = 2/44 (4%)
Frame = +2
Query: 20 VLSEALCL--RWAIGMARDLGFRHVWFDTDCLVLFDYWRRSDEG 61
V +A+CL RW +G+A +VW+ + L WRR D G
Sbjct: 599 VRCDAICLIIRWRVGIAYRRAQHNVWYSYELCDLEIAWRRIDSG 730
>AJ497561 weakly similar to GP|10140689|gb putative non-LTR retroelement
reverse transcriptase {Oryza sativa (japonica
cultivar-group)}, partial (2%)
Length = 621
Score = 31.2 bits (69), Expect = 0.48
Identities = 15/29 (51%), Positives = 23/29 (78%)
Frame = +3
Query: 82 KNQGGLGVRSTRESNVSLLGKLAWKLGSR 110
K+ GGLG+++ +E N+S+LGK WKL S+
Sbjct: 324 KSVGGLGLQN-QEFNISMLGKQRWKLLSQ 407
>BQ144198
Length = 1200
Score = 28.9 bits (63), Expect = 2.4
Identities = 15/54 (27%), Positives = 27/54 (49%)
Frame = -1
Query: 72 CVNWKQIVLPKNQGGLGVRSTRESNVSLLGKLAWKLGSRIFITKYLKGSSIFSY 125
CV I+ + G + VRS R + + ++ +L W S ++ Y+ S FS+
Sbjct: 807 CVTSSIIIYAYHAGSISVRSQRATRLRIISRLLWGDASSFYVC-YVIQVSFFSF 649
>BG586720 similar to GP|19070477|gb PTH-2 {Cucumis melo}, partial (37%)
Length = 603
Score = 28.9 bits (63), Expect = 2.4
Identities = 16/46 (34%), Positives = 24/46 (51%), Gaps = 1/46 (2%)
Frame = +3
Query: 171 LCALVPFVHISDTALTISDVWVEGSWNLSMLYTELPDPY-KPTITS 215
LCA P V S+ VW + +N ++ E+ DP+ K +ITS
Sbjct: 246 LCARPPLVRSSEKKKASLVVWFQKCYNEGVIIEEMVDPFIKDSITS 383
>TC81272 similar to PIR|T05225|T05225 extensin homolog F17I5.160 -
Arabidopsis thaliana, partial (3%)
Length = 757
Score = 28.5 bits (62), Expect = 3.1
Identities = 13/45 (28%), Positives = 19/45 (41%), Gaps = 5/45 (11%)
Frame = +2
Query: 206 PDPYKPTITSMQGPSHETVDDCWVW-----NSNPAGYSCSDAYQW 245
PDP++P S P + W W ++ P + CS A W
Sbjct: 119 PDPHRPRWCSPARPRCQRCSSAWPWCFRCSSARPWCFRCSPARSW 253
>TC80388
Length = 1112
Score = 28.5 bits (62), Expect = 3.1
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = +2
Query: 19 PVLSEALCLRWAIGMARDLGFRHVWFDTDCLVLFD 53
P + EAL +R + +ARD G + + TD LV+ D
Sbjct: 716 PCMVEALGVR*CLQLARDQGLQKLEIQTDALVIAD 820
>BF633993 homologue to GP|22135972|g putative WD-40 repeat protein
{Arabidopsis thaliana}, partial (9%)
Length = 438
Score = 27.7 bits (60), Expect = 5.3
Identities = 10/26 (38%), Positives = 16/26 (61%), Gaps = 1/26 (3%)
Frame = +1
Query: 226 DCWV-WNSNPAGYSCSDAYQWLRDWT 250
+CWV W+++ GY Y +R+WT
Sbjct: 190 ECWV*WSNHCVGYMGGHTYSNIRNWT 267
>AI974304 similar to GP|12325083|gb| hypothetical protein; 3218-172
{Arabidopsis thaliana}, partial (12%)
Length = 529
Score = 27.7 bits (60), Expect = 5.3
Identities = 13/44 (29%), Positives = 19/44 (42%)
Frame = +1
Query: 222 ETVDDCWVWNSNPAGYSCSDAYQWLRDWTVFRVLSVLGSMSSII 265
E VDD W G++C Y W T+ +S SM ++
Sbjct: 28 EAVDDEWPLGGQVNGFACLLTYLWKP*QTILLEISTKASMECLV 159
>AL378562
Length = 510
Score = 27.3 bits (59), Expect = 6.9
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = +3
Query: 222 ETVDDCWVWNSNPAGYSCSD 241
E CW WN + A +SCSD
Sbjct: 231 EPFQKCWNWNYSCAHHSCSD 290
>BI263281 weakly similar to GP|498038|gb|A lipid transfer protein {Senecio
odorus}, partial (28%)
Length = 681
Score = 27.3 bits (59), Expect = 6.9
Identities = 15/46 (32%), Positives = 19/46 (40%), Gaps = 4/46 (8%)
Frame = +2
Query: 193 EGSWNLSMLYTELPDPYKPTITSMQGPSH----ETVDDCWVWNSNP 234
E SW LS P+ + T G + V DC WNS+P
Sbjct: 449 EVSWTLSFHQLPFTSPFNSSTTVSNGGNRYGFGAPVSDCSNWNSDP 586
>TC78696 similar to GP|15450733|gb|AAK96638.1 At2g36220/F2H17.17
{Arabidopsis thaliana}, partial (13%)
Length = 1108
Score = 27.3 bits (59), Expect = 6.9
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = -2
Query: 17 LTPVLSEALCLRWAIGMARDLGFRHV 42
L P++ + LC RW + R LGFR++
Sbjct: 393 LHPLVRKRLCCRWRRRIRRKLGFRNL 316
>TC88933 weakly similar to GP|17381142|gb|AAL36383.1 unknown protein
{Arabidopsis thaliana}, partial (73%)
Length = 1632
Score = 26.9 bits (58), Expect = 9.1
Identities = 12/42 (28%), Positives = 20/42 (47%)
Frame = +1
Query: 150 SFAIHDGCTSFWYSDWTGLGKLCALVPFVHISDTALTISDVW 191
S + H GC+ +WY + C VH+++ IS +W
Sbjct: 313 SLS*HGGCSGWWYFN-------CYSSSLVHLTEDFEEISQIW 417
>AW774658 similar to GP|2808681|emb| Hcr9-4B {Lycopersicon hirsutum}, partial
(4%)
Length = 665
Score = 26.9 bits (58), Expect = 9.1
Identities = 16/45 (35%), Positives = 24/45 (52%), Gaps = 1/45 (2%)
Frame = -1
Query: 20 VLSEALCLRWAIGMARDLGFRHVWFDTDCLVL-FDYWRRSDEGRS 63
+LS ++CL +IGM F H+ F D L+L W + G+S
Sbjct: 443 LLSHSICLNLSIGMHSLTVFSHLQFADDTLLLGVKSWANAPCGQS 309
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.136 0.446
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,420,792
Number of Sequences: 36976
Number of extensions: 197928
Number of successful extensions: 895
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 888
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 895
length of query: 287
length of database: 9,014,727
effective HSP length: 95
effective length of query: 192
effective length of database: 5,502,007
effective search space: 1056385344
effective search space used: 1056385344
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0083.3