BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0058a.9
(407 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77695 similar to PIR|T09704|T09704 probable arginine/serine-ri... 44 1e-04
TC79604 similar to GP|18175762|gb|AAL59923.1 unknown protein {Ar... 43 3e-04
TC77694 homologue to PIR|T09704|T09704 probable arginine/serine-... 42 3e-04
TC76886 similar to PIR|T01932|T01932 RNA binding protein homolog... 40 0.001
TC76331 similar to GP|7673355|gb|AAF66823.1| poly(A)-binding pro... 38 0.006
TC76341 similar to GP|7528270|gb|AAF63202.1| poly(A)-binding pro... 38 0.006
AL371197 homologue to GP|6273331|gb| glycine-rich RNA binding pr... 37 0.011
TC77756 similar to PIR|T06796|T06796 glycine-rich RNA-binding pr... 37 0.011
BI266384 37 0.018
TC89001 similar to GP|5726567|gb|AAD48471.1| glycine-rich RNA-bi... 37 0.018
AL374421 similar to PIR|S59529|S59 RNA-binding glycine-rich prot... 37 0.018
AL388260 weakly similar to SP|Q08937|ROC2_ 29 kDa ribonucleoprot... 36 0.024
TC86398 similar to GP|16226341|gb|AAL16140.1 AT3g11400/F24K9_7 {... 36 0.031
TC89204 similar to GP|16226341|gb|AAL16140.1 AT3g11400/F24K9_7 {... 36 0.031
TC88545 similar to GP|13560783|gb|AAK30205.1 poly(A)-binding pro... 36 0.031
AJ503118 similar to GP|18175762|gb| unknown protein {Arabidopsis... 35 0.052
TC79394 weakly similar to PIR|T49019|T49019 probable RNA binding... 35 0.052
CA859237 weakly similar to PIR|T45886|T458 RNA-binding protein c... 35 0.052
BE124733 similar to PIR|T06796|T06 glycine-rich RNA-binding prot... 35 0.052
TC86510 similar to GP|20160777|dbj|BAB89718. putative ribonucleo... 35 0.068
>TC77695 similar to PIR|T09704|T09704 probable arginine/serine-rich splicing
factor - alfalfa, partial (83%)
Length = 1657
Score = 43.5 bits (101), Expect = 1e-04
Identities = 20/55 (36%), Positives = 32/55 (57%)
Frame = +1
Query: 82 KFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAIQGLNGFRLGSAPLSASLA 136
K+GKVV++F+ R R++G F F RY D+A KA+ L+G + ++ A
Sbjct: 178 KYGKVVDIFIPRDRRTGESRGFAFVRYKYADEASKAVDRLDGRMVDGREITVQFA 342
>TC79604 similar to GP|18175762|gb|AAL59923.1 unknown protein {Arabidopsis
thaliana}, partial (56%)
Length = 727
Score = 42.7 bits (99), Expect = 3e-04
Identities = 22/63 (34%), Positives = 35/63 (54%)
Frame = +2
Query: 61 SVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAIQG 120
++F+ G+ + LR+ KFG+VV+ V R SG FGF +Y + A K I+G
Sbjct: 242 NLFVSGLSKRTTTETLREAFQKFGEVVHARVVTDRVSGYSKGFGFVKYATLEDAAKGIEG 421
Query: 121 LNG 123
++G
Sbjct: 422 MDG 430
>TC77694 homologue to PIR|T09704|T09704 probable arginine/serine-rich
splicing factor - alfalfa, partial (95%)
Length = 1494
Score = 42.4 bits (98), Expect = 3e-04
Identities = 19/55 (34%), Positives = 32/55 (57%)
Frame = +3
Query: 82 KFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAIQGLNGFRLGSAPLSASLA 136
K+GKVV++F+ + R++G F F RY D+A KA+ L+G + ++ A
Sbjct: 150 KYGKVVDIFIPKDRRTGESRGFAFVRYKYADEASKAVDRLDGRMVDGREITVQFA 314
>TC76886 similar to PIR|T01932|T01932 RNA binding protein homolog - common
tobacco (fragment), partial (67%)
Length = 1896
Score = 40.4 bits (93), Expect = 0.001
Identities = 33/128 (25%), Positives = 53/128 (40%), Gaps = 10/128 (7%)
Frame = +2
Query: 45 ADGGSRSCSAGRRSCFSVFIDGIE-ESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRF 103
A GG S+ S SVF+ + + D + SKF + V +GR +
Sbjct: 797 AGGGGERRSSEATSDLSVFVGDLAIDVTDAMLQETFASKFSSIKGAKVVIDSNTGRSKGY 976
Query: 104 GFFRYGLRDQALKAIQGLNGFRLGSAPLSASLAWAPWVCGSPRR---------GFGLCSQ 154
GF R+G + +A+ +NG S P+ +A G+P++ G G S
Sbjct: 977 GFVRFGDESERTRAMTEMNGVYCSSRPMRVGVATPKKTYGNPQQYSSQAVVLAGGGGHSN 1156
Query: 155 ASSAQGQR 162
+ AQG +
Sbjct: 1157GAMAQGSQ 1180
Score = 35.4 bits (80), Expect = 0.040
Identities = 28/102 (27%), Positives = 47/102 (45%)
Frame = +2
Query: 38 GGELDGEADGGSRSCSAGRRSCFSVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKS 97
GG +G GS+S G + ++F+ G++ LR +FG V+++ + +
Sbjct: 1142 GGHSNGAMAQGSQS--EGDSNNTTIFVGGLDSDISDEDLRQPFLQFGDVISVKIPVGKGC 1315
Query: 98 GRFFRFGFFRYGLRDQALKAIQGLNGFRLGSAPLSASLAWAP 139
G F + R A +AIQGLNG +G + S +P
Sbjct: 1316 G------FVQLADRKNAEEAIQGLNGTVIGKQTVRLSWGRSP 1423
Score = 33.5 bits (75), Expect = 0.15
Identities = 18/78 (23%), Positives = 37/78 (47%)
Frame = +2
Query: 61 SVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAIQG 120
++++ + D L + + G+V + V R +++G+ +GF + R A K +Q
Sbjct: 551 TIWLGDLHHWMDETFLHNCFAHTGEVASAKVIRNKQTGQSEGYGFVEFYTRAMAEKVLQN 730
Query: 121 LNGFRLGSAPLSASLAWA 138
NG + + + L WA
Sbjct: 731 FNGTMMPNTDQAFRLNWA 784
>TC76331 similar to GP|7673355|gb|AAF66823.1| poly(A)-binding protein
{Nicotiana tabacum}, partial (97%)
Length = 2581
Score = 38.1 bits (87), Expect = 0.006
Identities = 21/66 (31%), Positives = 37/66 (55%)
Frame = +3
Query: 61 SVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAIQG 120
++FI ++++ D+ L D S FG +++ V SG+ +GF ++ + A KAI+
Sbjct: 711 NIFIKNLDKAIDHKALHDTFSSFGNILSCKVAVDG-SGQSKGYGFVQFDTEEAAQKAIEK 887
Query: 121 LNGFRL 126
LNG L
Sbjct: 888 LNGMLL 905
Score = 36.2 bits (82), Expect = 0.024
Identities = 23/75 (30%), Positives = 38/75 (50%), Gaps = 1/75 (1%)
Frame = +3
Query: 53 SAGRRSCFS-VFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLR 111
S G R+ F+ VF+ + ES L+ +FG + + V R G+ FGF +
Sbjct: 957 STGDRAKFNNVFVKNLSESTTDDELKKTFGEFGTITSAVVMRD-GDGKSKCFGFVNFEST 1133
Query: 112 DQALKAIQGLNGFRL 126
D A +A++ LNG ++
Sbjct: 1134 DDAARAVEALNGKKI 1178
>TC76341 similar to GP|7528270|gb|AAF63202.1| poly(A)-binding protein
{Cucumis sativus}, partial (90%)
Length = 2501
Score = 38.1 bits (87), Expect = 0.006
Identities = 21/74 (28%), Positives = 39/74 (52%)
Frame = +3
Query: 50 RSCSAGRRSCFSVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYG 109
R S+ + ++FI ++++ D+ L D S FG++++ + SG+ +GF ++
Sbjct: 528 RDPSSRKSGTANIFIKNLDKTIDHKALHDTFSSFGQIMSCKIATD-GSGQSKGYGFVQFE 704
Query: 110 LRDQALKAIQGLNG 123
D A AI LNG
Sbjct: 705 AEDSAQNAIDKLNG 746
Score = 33.5 bits (75), Expect = 0.15
Identities = 18/63 (28%), Positives = 32/63 (50%)
Frame = +3
Query: 61 SVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAIQG 120
+V++ + ESF L++ +G + + + R GR FGF + + A KA++
Sbjct: 834 NVYVKNLSESFTEDDLKNEFGAYGTITSAVLMRDA-DGRSKCFGFVNFENAEDAAKAVEA 1010
Query: 121 LNG 123
LNG
Sbjct: 1011LNG 1019
>AL371197 homologue to GP|6273331|gb| glycine-rich RNA binding protein
{Medicago sativa}, complete
Length = 437
Score = 37.4 bits (85), Expect = 0.011
Identities = 24/101 (23%), Positives = 44/101 (42%)
Frame = +1
Query: 60 FSVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAIQ 119
+ F+ G+ + D L S++G++V+ + R++GR FGF + AI+
Sbjct: 58 YRCFVGGLAWATDNEALEKAFSQYGEIVDSKIINDRETGRSRGFGFVTFANEKSMNDAIE 237
Query: 120 GLNGFRLGSAPLSASLAWAPWVCGSPRRGFGLCSQASSAQG 160
+NG L ++ + A + + G G G C G
Sbjct: 238 AMNGQDLDGRNITVNQAQSRGIGGGGGGGPGGCGYRGGGVG 360
>TC77756 similar to PIR|T06796|T06796 glycine-rich RNA-binding protein -
garden pea, partial (89%)
Length = 813
Score = 37.4 bits (85), Expect = 0.011
Identities = 29/93 (31%), Positives = 42/93 (44%)
Frame = +3
Query: 58 SCFSVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKA 117
S +FI G+ + D LRD + +G VV V R++GR FGF + + A A
Sbjct: 180 SSSKLFIGGLSYNVDDQSLRDAFTTYGDVVEARVITDRETGRSRGFGFVNFTSEESATSA 359
Query: 118 IQGLNGFRLGSAPLSASLAWAPWVCGSPRRGFG 150
+ ++G L + S A G PR G G
Sbjct: 360 L-SMDGQDLNGRNIRVSYA-NDRQAGGPRPGGG 452
>BI266384
Length = 626
Score = 36.6 bits (83), Expect = 0.018
Identities = 20/66 (30%), Positives = 33/66 (49%)
Frame = +2
Query: 58 SCFSVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKA 117
S +FI G+ S LHL + S FG+V + + +K+G+ F + + D A A
Sbjct: 203 SSTKIFIKGLPLSTTELHLTKVFSMFGEVTQVKLLIDKKTGQSLGFAYIWFVEEDSAQSA 382
Query: 118 IQGLNG 123
+ +NG
Sbjct: 383 AKEMNG 400
>TC89001 similar to GP|5726567|gb|AAD48471.1| glycine-rich RNA-binding
protein {Glycine max}, partial (90%)
Length = 488
Score = 36.6 bits (83), Expect = 0.018
Identities = 24/91 (26%), Positives = 41/91 (44%)
Frame = +3
Query: 60 FSVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAIQ 119
+ F+ G+ + D L SKFG++ + V R++GR FGF + AI+
Sbjct: 51 YRCFVGGLAWATDSQALEQAFSKFGEITDSKVINDRETGRSRGFGFVTFAEEKSMRDAIE 230
Query: 120 GLNGFRLGSAPLSASLAWAPWVCGSPRRGFG 150
+NG + ++ + A + G R G G
Sbjct: 231 EMNGQDIDGRNITVNEAQSRGSGGGGRGGGG 323
>AL374421 similar to PIR|S59529|S59 RNA-binding glycine-rich protein-1
(RGP-1c) - wood tobacco, partial (62%)
Length = 310
Score = 36.6 bits (83), Expect = 0.018
Identities = 22/91 (24%), Positives = 43/91 (47%)
Frame = +2
Query: 60 FSVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAIQ 119
+ F+ G+ + D L S++G++++ + R++GR FGF + AI+
Sbjct: 14 YRCFVGGLAWATDSEALEKAFSQYGEIIDSKIINDRETGRSRGFGFVTFADEKSMRDAIE 193
Query: 120 GLNGFRLGSAPLSASLAWAPWVCGSPRRGFG 150
G+NG + ++ + A + G RG G
Sbjct: 194 GMNGQDMDGRNITVNEAQSRGSGGGGGRGGG 286
>AL388260 weakly similar to SP|Q08937|ROC2_ 29 kDa ribonucleoprotein B
chloroplast precursor (CP29B). [Wood tobacco] {Nicotiana
sylvestris}, partial (26%)
Length = 346
Score = 36.2 bits (82), Expect = 0.024
Identities = 21/61 (34%), Positives = 31/61 (50%)
Frame = +3
Query: 62 VFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAIQGL 121
+F+ G+ D LR ++G V + V R R +GR FGF + ++A AIQ L
Sbjct: 90 LFVGGLSWGTDDRTLRSKFEEYGTVEDAVVIRDRDTGRSRGFGFVTFASNEEAEIAIQNL 269
Query: 122 N 122
N
Sbjct: 270 N 272
>TC86398 similar to GP|16226341|gb|AAL16140.1 AT3g11400/F24K9_7 {Arabidopsis
thaliana}, partial (75%)
Length = 1170
Score = 35.8 bits (81), Expect = 0.031
Identities = 24/78 (30%), Positives = 37/78 (46%)
Frame = +3
Query: 61 SVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAIQG 120
SV + + E L ++ FG V ++V +K+G FGF + R+ A +AI
Sbjct: 690 SVRVTNLSEDTREPDLLELFRPFGAVSRVYVAIDQKTGMSRGFGFVNFVSREDAQRAINK 869
Query: 121 LNGFRLGSAPLSASLAWA 138
LNG+ G L + WA
Sbjct: 870 LNGY--GYDNLILRVEWA 917
>TC89204 similar to GP|16226341|gb|AAL16140.1 AT3g11400/F24K9_7 {Arabidopsis
thaliana}, partial (80%)
Length = 1087
Score = 35.8 bits (81), Expect = 0.031
Identities = 23/78 (29%), Positives = 36/78 (45%)
Frame = +1
Query: 61 SVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAIQG 120
SV + + E L ++ FG V ++V + +G FGF + R+ A +AI
Sbjct: 616 SVRVTNLSEDTREPDLHELFGTFGSVSRVYVAIDKNTGTSRGFGFVNFVNREDAQRAINK 795
Query: 121 LNGFRLGSAPLSASLAWA 138
LNG+ G L + WA
Sbjct: 796 LNGY--GYDNLILKVEWA 843
>TC88545 similar to GP|13560783|gb|AAK30205.1 poly(A)-binding protein
{Daucus carota}, partial (35%)
Length = 866
Score = 35.8 bits (81), Expect = 0.031
Identities = 22/82 (26%), Positives = 38/82 (45%)
Frame = +2
Query: 50 RSCSAGRRSCFSVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYG 109
R S + ++FI ++ + D+ L D S FG +++ + SG +GF ++
Sbjct: 413 RDPSVRKSGAANIFIKNLDRAIDHKALYDTFSIFGNILSCKIAMDA-SGLSKGYGFVQFE 589
Query: 110 LRDQALKAIQGLNGFRLGSAPL 131
+ A AI LNG L P+
Sbjct: 590 NEESAQSAIDKLNGMLLNDKPV 655
>AJ503118 similar to GP|18175762|gb| unknown protein {Arabidopsis thaliana},
partial (40%)
Length = 377
Score = 35.0 bits (79), Expect = 0.052
Identities = 20/57 (35%), Positives = 28/57 (49%)
Frame = +1
Query: 62 VFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAI 118
VF+ G+ + LR+ KFG+V + V R SG FG+ RY + A K I
Sbjct: 205 VFVTGLNKRTTSETLREAFKKFGEVKQVRVVADRVSGYSKGFGYVRYATSEDAAKGI 375
>TC79394 weakly similar to PIR|T49019|T49019 probable RNA binding protein -
Arabidopsis thaliana, partial (74%)
Length = 1909
Score = 35.0 bits (79), Expect = 0.052
Identities = 16/76 (21%), Positives = 35/76 (46%)
Frame = +2
Query: 61 SVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAIQG 120
+V++ + E+ L+++ GK+ + + + R+GF + R A+KA++
Sbjct: 956 AVYVKNLPENITQNRLKELFEHHGKITKVALPPAKAGQEKSRYGFVHFADRSSAMKALKN 1135
Query: 121 LNGFRLGSAPLSASLA 136
+ + L SLA
Sbjct: 1136 TEKYEINGQTLECSLA 1183
>CA859237 weakly similar to PIR|T45886|T458 RNA-binding protein cp29 protein
- Arabidopsis thaliana, partial (20%)
Length = 298
Score = 35.0 bits (79), Expect = 0.052
Identities = 18/41 (43%), Positives = 24/41 (57%)
Frame = +2
Query: 82 KFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKAIQGLN 122
+FG V + V R R++GR FGF Y ++A AIQ LN
Sbjct: 23 EFGNVEDAVVIRDRETGRSRGFGFVTYSSNEEAEAAIQSLN 145
>BE124733 similar to PIR|T06796|T06 glycine-rich RNA-binding protein - garden
pea, partial (86%)
Length = 580
Score = 35.0 bits (79), Expect = 0.052
Identities = 20/61 (32%), Positives = 30/61 (48%)
Frame = +3
Query: 58 SCFSVFIDGIEESFDYLHLRDICSKFGKVVNLFVQRQRKSGRFFRFGFFRYGLRDQALKA 117
S +FI G+ + D LRD + +G VV V R++GR FGF + + A A
Sbjct: 138 SSSKLFIGGLSYNVDDQSLRDAFTTYGDVVEARVITDRETGRSRGFGFVNFTSEESATSA 317
Query: 118 I 118
+
Sbjct: 318 L 320
>TC86510 similar to GP|20160777|dbj|BAB89718. putative ribonucleoprotein
{Oryza sativa (japonica cultivar-group)}, partial (67%)
Length = 1587
Score = 34.7 bits (78), Expect = 0.068
Identities = 28/98 (28%), Positives = 43/98 (43%), Gaps = 4/98 (4%)
Frame = +3
Query: 38 GGELDGEADGGSRSCSAGRRS----CFSVFIDGIEESFDYLHLRDICSKFGKVVNLFVQR 93
GG + GS S G ++ ++FI I + F L + FG+V++ V
Sbjct: 1116 GGNGNSATSSGSSKNSGGGQAEGPPGANLFIYHIPQEFGDQELANAFQPFGRVLSAKVFV 1295
Query: 94 QRKSGRFFRFGFFRYGLRDQALKAIQGLNGFRLGSAPL 131
+ +G FGF Y + A AI +NG +LG L
Sbjct: 1296 DKATGVSKCFGFVSYDSPEAAQSAISMMNGCQLGGKKL 1409
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.142 0.455
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,452,104
Number of Sequences: 36976
Number of extensions: 241426
Number of successful extensions: 2163
Number of sequences better than 10.0: 91
Number of HSP's better than 10.0 without gapping: 2107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2161
length of query: 407
length of database: 9,014,727
effective HSP length: 98
effective length of query: 309
effective length of database: 5,391,079
effective search space: 1665843411
effective search space used: 1665843411
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0058a.9