BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0369a.2
(1602 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 194 1e-50
BP084332 184 1e-46
BP052756 86 5e-17
TC10598 weakly similar to GB|AAM78036.1|21928015|AY125526 At2g01... 38 0.011
BP037005 37 0.024
BP065763 35 0.093
TC8412 weakly similar to UP|Q9LW00 (Q9LW00) Genomic DNA, chromos... 35 0.12
TC8557 similar to UP|Q9LK52 (Q9LK52) Dbj|BAA90629.1, complete 34 0.21
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 34 0.21
BP039068 33 0.27
TC13011 similar to UP|Q9JM93 (Q9JM93) SRp25 nuclear protein, par... 33 0.46
TC17929 33 0.46
NP459554 RNA polymerase alpha subunit [Lotus japonicus] 32 0.60
AV770327 32 0.79
TC9596 similar to UP|AAR24722 (AAR24722) At5g49400, partial (66%) 32 1.0
TC8353 homologue to UP|HS7M_PHAVU (Q01899) Heat shock 70 kDa pro... 32 1.0
TC20039 similar to UP|O81652 (O81652) Phyb1, partial (14%) 31 1.3
TC14088 similar to UP|RLA0_SOYBN (P50346) 60S acidic ribosomal p... 30 2.3
BP071259 30 2.3
BP034655 30 2.3
>BP066094
Length = 532
Score = 194 bits (493), Expect(3) = 1e-50
Identities = 109/142 (76%), Positives = 119/142 (83%)
Frame = +3
Query: 1187 DC*SPSQ*ITI*FYIRWT*RVPS*MVISQRKSMFINPQVLKMKRNQTMCSN*RNHSTV*S 1246
D *S SQ*IT *F+IR RVP *MV SQRKSMFINPQV KM+R T+ SN*RN S V*S
Sbjct: 105 DY*SHSQ*ITT*FFIR*MLRVPF*MVTSQRKSMFINPQVXKMRRTLTIFSN*RNLSMV*S 284
Query: 1247 KLPEHGMRDLAHSFWRMNL*GVK*IQLFFARLIKMIS*LCKFMLMILYLVLLINLYAKNF 1306
KLPEHGMRD AHSFW+M+ *GVK*IQL FA+L KMI *LCKFMLMILYL LLI+LYAK+F
Sbjct: 285 KLPEHGMRDSAHSFWKMSX*GVK*IQLSFAKLTKMIY*LCKFMLMILYLDLLIHLYAKSF 464
Query: 1307 LR*CRLNLR*V*WEN*STFWEY 1328
LR*CRLNL+ V*WEN +TFW Y
Sbjct: 465 LR*CRLNLKCV*WENSNTFWVY 530
Score = 25.8 bits (55), Expect(3) = 1e-50
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = +1
Query: 1161 LVKATASRKK*TTLKHLLR*QD 1182
+ + T S K+*T LK LLR*QD
Sbjct: 28 VAQGTVSSKE*TILKRLLR*QD 93
Score = 19.2 bits (38), Expect(3) = 1e-50
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = +2
Query: 1155 ETRQG*LVKATAS 1167
ETRQG*L+K ++
Sbjct: 11 ETRQG*LLKVQSA 49
>BP084332
Length = 368
Score = 184 bits (466), Expect = 1e-46
Identities = 104/116 (89%), Positives = 105/116 (89%)
Frame = +3
Query: 1481 QARGNQPLHYQLQRQNISQQQYAALRCSG*NISWRIIRSLRAISQFIVITLLQSR*ARIL 1540
Q RGNQPLHY LQRQNISQQQYAALRCSG*NISWRII+SLRAISQ IVITLLQ * RIL
Sbjct: 21 QLRGNQPLHYLLQRQNISQQQYAALRCSG*NISWRIIKSLRAISQSIVITLLQFH*VRIL 200
Query: 1541 SCIQGQSTLR*SITSLEIMCRRAYFL*SLLILTINGQISLQSP*QRIDLILF*KI* 1596
S IQGQSTLR*SIT LEIM RRAYF *SLLILTINGQISLQSP*QRIDLI F*KI*
Sbjct: 201 SYIQGQSTLR*SITLLEIMYRRAYFF*SLLILTINGQISLQSP*QRIDLISF*KI* 368
>BP052756
Length = 488
Score = 85.9 bits (211), Expect = 5e-17
Identities = 53/124 (42%), Positives = 83/124 (66%), Gaps = 2/124 (1%)
Frame = +1
Query: 292 KKPKKHFKTKKSLMVTFDESESEDVDS--DGEVQGLMAIVKDKGAESKEAVDSDSESEGD 349
K+P+K F TKK+L +TFD ++S++ +S D V+ LMA +GAE+ SE D
Sbjct: 151 KRPRKVF-TKKAL-ITFDATDSDEAESEEDEAVEALMATTS-RGAEA---------SEDD 294
Query: 350 PNSDDENEVFASFSTSELKHALSDIMDKYNSLLSTHKKLKKNLSAVSKTPSEHEKIISDL 409
+S++++EVF+ FS SEL+ ALS+IM K++ LL+ HK+LKK + + + HEK +S+L
Sbjct: 295 SDSENDDEVFSDFSLSELRTALSEIMKKHDILLNKHKELKKKYAVKTSSSEVHEKSVSEL 474
Query: 410 KNEN 413
+EN
Sbjct: 475 IDEN 486
>TC10598 weakly similar to GB|AAM78036.1|21928015|AY125526
At2g01100/F23H14.7 {Arabidopsis thaliana;}, partial
(33%)
Length = 902
Score = 38.1 bits (87), Expect = 0.011
Identities = 31/118 (26%), Positives = 49/118 (41%), Gaps = 1/118 (0%)
Frame = +3
Query: 183 SEMQDLKKKSIALKSKSEKAKVEKSKA-LQAEEEESEEASEDSDEDELTLISKRLNRIWK 241
SE D +S+ E+ + K K L+ + S+ + DS DE +SKR + +
Sbjct: 555 SESSDFSGDESESRSEEERRRKRKQKKKLRDQVSRSDSSDSDSSADE---VSKRKRQHKR 725
Query: 242 HRQSKFKGSGRAKGKYESSGQKKSSIKEVTCFECKESGHYKSDCPKLKKDKKPKKHFK 299
HR +K GS + + S +K+S K ES + K +KH K
Sbjct: 726 HRVTKSSGSDFSSDEENSPVRKRSHGKHHKRHRHSESSESDLSSDDIHHKKSHRKHHK 899
Score = 33.9 bits (76), Expect = 0.21
Identities = 46/174 (26%), Positives = 72/174 (40%), Gaps = 7/174 (4%)
Frame = +3
Query: 330 KDKGAESKEAVDSDSESEGDPNSDDENEVFASFSTSELKHALSDIMDKYNSLLSTHKKLK 389
K K A+ K+ DSDSES+ D SDDE++ TS H Y+ S H+K K
Sbjct: 369 KLKAAKHKKRADSDSESDYD--SDDESK-----RTSRRSHRKHKKRSHYD---SDHEKRK 518
Query: 390 KNLS--AVSKTPSEHEKIISDLKNENHALVNSNSVLKNQIAKLEEIVACDASDCRNE--- 444
+ LS K SE D ++E+ + K + +++ D+SD +
Sbjct: 519 EKLSKRKTKKWSSESSDFSGD-ESESRSEEERRRKRKQKKKLRDQVSRSDSSDSDSSADE 695
Query: 445 -SKYEKSFQRF-LAKSVDRSLMASMIYGVSRNGMHGIGYSKPIRNEPSVSKAKS 496
SK ++ +R + KS + R HG + + +E S S S
Sbjct: 696 VSKRKRQHKRHRVTKSSGSDFSSDEENSPVRKRSHGKHHKRHRHSESSESDLSS 857
>BP037005
Length = 569
Score = 37.0 bits (84), Expect = 0.024
Identities = 46/165 (27%), Positives = 69/165 (40%), Gaps = 9/165 (5%)
Frame = +2
Query: 221 SEDSDEDELTLISKRLNRIWKHRQSKFKGSGRAKGKYESSGQKKSSIKEVTCFECKESGH 280
SE SD+DE I LN R K K K + K+ K+V FE S
Sbjct: 32 SESSDDDE---IENHLNS----RSDKEFFDALVKVKKQDPVLKQ---KDVKLFESDHSSE 181
Query: 281 YKSDCPKLK-----KDKKPKKHF---KTKKSLMVTFDESESEDVDSDGEVQGLMAIVKDK 332
+SD K KDKKPK + T + L+ + + E+ + + E +G + I KDK
Sbjct: 182 DESDENKKSKSKDMKDKKPKPMYLKDVTARHLIEDGPDFDDEEEEINEEEKGKLEISKDK 361
Query: 333 GA-ESKEAVDSDSESEGDPNSDDENEVFASFSTSELKHALSDIMD 376
A K+ EG +++ + ELK AL + ++
Sbjct: 362 KAIRLKDKKTKHLVLEGSSXVNEDGKKTYGEEQEELKKALQEAVE 496
>BP065763
Length = 537
Score = 35.0 bits (79), Expect = 0.093
Identities = 18/46 (39%), Positives = 27/46 (58%), Gaps = 1/46 (2%)
Frame = -1
Query: 245 SKFKGSGRAKGKYESSGQKKSSI-KEVTCFECKESGHYKSDCPKLK 289
SK K + + K ++ K+ I KE C+ CK++GH+K CPK K
Sbjct: 465 SKAKPGRKEQKKDQALKVKEGRIHKEHVCYFCKKAGHFKKYCPKRK 328
>TC8412 weakly similar to UP|Q9LW00 (Q9LW00) Genomic DNA, chromosome 3, P1
clone: MSJ11, partial (39%)
Length = 1403
Score = 34.7 bits (78), Expect = 0.12
Identities = 30/136 (22%), Positives = 55/136 (40%), Gaps = 27/136 (19%)
Frame = +3
Query: 246 KFKGSGRAKGKYESSGQKKSSIKEVTCFECKESGHYKSDCPKLKKDKKPK-----KHFKT 300
K +G + K ++SG+++ +E E +E+G K + + + K + + K
Sbjct: 384 KKRGKRSSASKKDASGEEEK--EETKDVEMQEAGETKDAVKENQDENKAEDTDVNQDEKK 557
Query: 301 KKSLMVTFDESESEDVD----------------------SDGEVQGLMAIVKDKGAESKE 338
++ DE +ED D +D E+Q V DKGAE +
Sbjct: 558 AEATDANQDEKRAEDADVKEPTHPGENTDGPNDEGKLNTADEELQASKEKVDDKGAEGSD 737
Query: 339 AVDSDSESEGDPNSDD 354
AV + + G+P +
Sbjct: 738 AVQNKDDGSGEPEKSE 785
>TC8557 similar to UP|Q9LK52 (Q9LK52) Dbj|BAA90629.1, complete
Length = 1064
Score = 33.9 bits (76), Expect = 0.21
Identities = 53/223 (23%), Positives = 93/223 (40%), Gaps = 16/223 (7%)
Frame = +3
Query: 253 AKGKYESSG-------QKKSS--IKEVTCFECKESGHYKSDCPKLKK-----DKKPKKHF 298
AKG E G QK SS I T + + G D K + +++ ++HF
Sbjct: 186 AKGGNEQGGTRIFGPSQKYSSRDIASHTTLKPRRDGQDTQDELKRRNLRDELEERERRHF 365
Query: 299 KTKKSLMVTFDESESEDVDSDGEVQGLMAIVKDK-GAESKEAVDSDSESEGDPNSDDENE 357
+K ++ + S ++G V+D A S +A DSD E + D SDD++
Sbjct: 366 SSKNK---SYSDDRDHGKSSHLFLEGTKRDVEDHIVARSVDADDSDVEVKSDEESDDDD- 533
Query: 358 VFASFSTSELKHALSDIMDKYNSLLSTHKKLKKNLSAVSKTPSEHEKIISDLKNENHALV 417
D D +LL+ +++KK A K E + +LK + L+
Sbjct: 534 ---------------DDEDDTEALLAELEQIKKE-RAEEKLRKERLEQEEELKVKEAELL 665
Query: 418 NSNSVLKNQIAKLEEIVACDASDCRNESKYE-KSFQRFLAKSV 459
N +L N + + D +N+++ E K+ +RF+ ++
Sbjct: 666 RGNPLLNNPASFNVKRRWDDDVVFKNQARGEAKALKRFINDTI 794
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 33.9 bits (76), Expect = 0.21
Identities = 20/76 (26%), Positives = 32/76 (41%), Gaps = 1/76 (1%)
Frame = +1
Query: 211 QAEEEESEEASEDSDEDEL-TLISKRLNRIWKHRQSKFKGSGRAKGKYESSGQKKSSIKE 269
+ E E E + + D + L K R+ SK G GR G+ ++
Sbjct: 1 ERERERERERERERERDAIHALDGKNGWRVELSHNSKGGGGGRGGGRGRGG-------ED 159
Query: 270 VTCFECKESGHYKSDC 285
+ C+EC E GH+ +C
Sbjct: 160 LKCYECGEPGHFAREC 207
>BP039068
Length = 467
Score = 33.5 bits (75), Expect = 0.27
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +3
Query: 259 SSGQKKSSIKEVTCFECKESGHYKSDCPKLKKDKKPKKH 297
SS ++ ++ C C+E+GH +DCP L + +H
Sbjct: 273 SSNVNHNNPRQTVCMNCQETGHASNDCPSLTLRSRNAQH 389
>TC13011 similar to UP|Q9JM93 (Q9JM93) SRp25 nuclear protein, partial (10%)
Length = 593
Score = 32.7 bits (73), Expect = 0.46
Identities = 21/68 (30%), Positives = 31/68 (44%)
Frame = -3
Query: 199 SEKAKVEKSKALQAEEEESEEASEDSDEDELTLISKRLNRIWKHRQSKFKGSGRAKGKYE 258
S+K K K + EEEE + D EDE+ ++ + + R K G GR K +
Sbjct: 543 SKKRKKAKEPSDSEEEEEEDAEESDHSEDEVNGEAE----VKRSRGGKTSGKGRGKAPKK 376
Query: 259 SSGQKKSS 266
S + K S
Sbjct: 375 SGAEVKGS 352
>TC17929
Length = 791
Score = 32.7 bits (73), Expect = 0.46
Identities = 15/36 (41%), Positives = 20/36 (54%)
Frame = +2
Query: 252 RAKGKYESSGQKKSSIKEVTCFECKESGHYKSDCPK 287
RA+ E+S + S TC+ C ESGH +CPK
Sbjct: 2 RAQIGEETSNRPNDSKFRQTCYRCGESGHKMRNCPK 109
>NP459554 RNA polymerase alpha subunit [Lotus japonicus]
Length = 1002
Score = 32.3 bits (72), Expect = 0.60
Identities = 20/68 (29%), Positives = 32/68 (46%)
Frame = +1
Query: 130 NKSYTTKDHVIRIIRCLPESWMPLVTSIELTRDVENMSLEELISILKCHELKRSEMQDLK 189
N S T K+ + R L + ++P + + E + EN + + + CH+L K
Sbjct: 616 NGSLTPKEALYEASRNLIDLFIPFLHAEEEKLNFENNEHKVTLPLFTCHDLLAKTKLKKK 795
Query: 190 KKSIALKS 197
KK IA KS
Sbjct: 796 KKEIAFKS 819
>AV770327
Length = 329
Score = 32.0 bits (71), Expect = 0.79
Identities = 15/42 (35%), Positives = 24/42 (56%)
Frame = -2
Query: 457 KSVDRSLMASMIYGVSRNGMHGIGYSKPIRNEPSVSKAKSLY 498
K +R +M M+ V+R+G+ IGY +P+ S KS+Y
Sbjct: 145 KGWEREMMTLMVSLVARDGIDWIGYQRPLTG*QSQGMLKSIY 20
>TC9596 similar to UP|AAR24722 (AAR24722) At5g49400, partial (66%)
Length = 1196
Score = 31.6 bits (70), Expect = 1.0
Identities = 38/164 (23%), Positives = 60/164 (36%)
Frame = +2
Query: 193 IALKSKSEKAKVEKSKALQAEEEESEEASEDSDEDELTLISKRLNRIWKHRQSKFKGSGR 252
I+ S++++ K K + + + E D E++ SK+ R KHR GS
Sbjct: 494 ISRPSRTQQLKNPKLRVNVSVSYDLENNDVDDKEEKAKSSSKKAKR--KHRSDSDSGSDS 667
Query: 253 AKGKYESSGQKKSSIKEVTCFECKESGHYKSDCPKLKKDKKPKKHFKTKKSLMVTFDESE 312
+E+ SS+ S SD + ++ ++ KK K KK S
Sbjct: 668 EDSVFETESGS-SSVTGSDYSSGSSSDSSSSDSEEERRRRRKKKTQKKKKGRRHKKYSSS 844
Query: 313 SEDVDSDGEVQGLMAIVKDKGAESKEAVDSDSESEGDPNSDDEN 356
SE SDSES D +SDD++
Sbjct: 845 SES--------------------------SDSESASDSDSDDKS 898
>TC8353 homologue to UP|HS7M_PHAVU (Q01899) Heat shock 70 kDa protein,
mitochondrial precursor, partial (74%)
Length = 1752
Score = 31.6 bits (70), Expect = 1.0
Identities = 21/55 (38%), Positives = 29/55 (52%), Gaps = 3/55 (5%)
Frame = +3
Query: 162 DVENMSLEELISILKCHELKRSEMQDLKKKSIAL---KSKSEKAKVEKSKALQAE 213
D +N L+ L+ E KR+E DL K +AL + +EKAK+E S Q E
Sbjct: 303 DFDNALLDFLVD-----EFKRTESIDLSKDKLALQRLREAAEKAKIELSSTSQTE 452
>TC20039 similar to UP|O81652 (O81652) Phyb1, partial (14%)
Length = 614
Score = 31.2 bits (69), Expect = 1.3
Identities = 20/98 (20%), Positives = 43/98 (43%)
Frame = +1
Query: 257 YESSGQKKSSIKEVTCFECKESGHYKSDCPKLKKDKKPKKHFKTKKSLMVTFDESESEDV 316
+E+ K++ +K VTC C +YK K + +K+ ++ + K++ + D+ +
Sbjct: 10 FEAGENKQALVKLVTCERCARKLNYKRQKEKEQLEKRQQEQDRRKRNRSKSDDDLDEFQE 189
Query: 317 DSDGEVQGLMAIVKDKGAESKEAVDSDSESEGDPNSDD 354
+ +G A + SD + + D N D+
Sbjct: 190 SQERRQKGKRATIS----------PSDHKIDDDDNIDE 273
>TC14088 similar to UP|RLA0_SOYBN (P50346) 60S acidic ribosomal protein P0,
partial (42%)
Length = 748
Score = 30.4 bits (67), Expect = 2.3
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = +2
Query: 185 MQDLKKKSIALKSKSEKAKVEKSKALQAEEEESEEASEDSDEDEL 229
++D K ++A + + A + A EEE+ E+ +E+SDED L
Sbjct: 278 LKDPSKFALAATAAAPAAAAAPAAAAAKEEEKKEDPAEESDEDGL 412
>BP071259
Length = 377
Score = 30.4 bits (67), Expect = 2.3
Identities = 23/74 (31%), Positives = 36/74 (48%), Gaps = 1/74 (1%)
Frame = -2
Query: 252 RAKGKYESSGQKKSSIKEVTCFECKESGHYKSDCPKLKKDKKPKKHFKTKK-SLMVTFDE 310
R GK+ + QKKS E T E + +K + K++ + PK+ KTKK ++
Sbjct: 289 RKSGKFTNRSQKKSKKSEFTTDE--KGDEHKINKIKIQIVESPKQEKKTKKIRNLLQ*RR 116
Query: 311 SESEDVDSDGEVQG 324
S+D D D + G
Sbjct: 115 GNSKDGDVDMSIDG 74
>BP034655
Length = 517
Score = 30.4 bits (67), Expect = 2.3
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = +1
Query: 196 KSKSEKAKVEKSKALQAEEEESEEASEDSDEDE 228
+ + E+ + E+ + + EEEE EE ED +EDE
Sbjct: 148 EEEEEEEEEEEEEEEEEEEEEEEEDEEDDEEDE 246
Score = 29.3 bits (64), Expect = 5.1
Identities = 16/52 (30%), Positives = 26/52 (49%), Gaps = 2/52 (3%)
Frame = +1
Query: 179 ELKRSEMQ--DLKKKSIALKSKSEKAKVEKSKALQAEEEESEEASEDSDEDE 228
E R+E+ L L E+ + E+ + + EEEE EE E+ DE++
Sbjct: 76 EFHRNEIAAAHLTAADFGLSDDDEEEEEEEEEEEEEEEEEEEEEEEEEDEED 231
Score = 29.3 bits (64), Expect = 5.1
Identities = 12/31 (38%), Positives = 20/31 (63%)
Frame = +1
Query: 198 KSEKAKVEKSKALQAEEEESEEASEDSDEDE 228
+ E+ + E+ + + EEEE EE ED ++DE
Sbjct: 145 EEEEEEEEEEEEEEEEEEEEEEEEEDEEDDE 237
Score = 28.5 bits (62), Expect = 8.7
Identities = 11/33 (33%), Positives = 21/33 (63%)
Frame = +1
Query: 196 KSKSEKAKVEKSKALQAEEEESEEASEDSDEDE 228
+ + E+ + E+ + + EEEE +E ++ DEDE
Sbjct: 154 EEEEEEEEEEEEEEEEEEEEEEDEEDDEEDEDE 252
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.355 0.156 0.545
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,580,652
Number of Sequences: 28460
Number of extensions: 364081
Number of successful extensions: 4526
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 3351
Number of HSP's successfully gapped in prelim test: 102
Number of HSP's that attempted gapping in prelim test: 1022
Number of HSP's gapped (non-prelim): 3648
length of query: 1602
length of database: 4,897,600
effective HSP length: 103
effective length of query: 1499
effective length of database: 1,966,220
effective search space: 2947363780
effective search space used: 2947363780
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.6 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0369a.2