BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0346.12
(1332 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP066094 126 7e-32
AV780009 125 3e-29
AV427921 120 1e-27
BP084332 104 8e-23
BP075943 98 1e-20
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 75 8e-18
TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprot... 57 1e-12
BP057233 69 5e-12
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 65 5e-11
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 65 9e-11
AV424544 64 2e-10
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 63 3e-10
AU088980 62 5e-10
BP043850 62 8e-10
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 55 6e-08
BP063694 54 2e-07
BP033602 53 4e-07
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 52 8e-07
AV410378 52 8e-07
BP052632 51 1e-06
>BP066094
Length = 532
Score = 126 bits (317), Expect(2) = 7e-32
Identities = 60/149 (40%), Positives = 96/149 (64%)
Frame = +2
Query: 907 ARLDTIRVILEVAAQFSWEVFQLDVKSAFLHGELKEEVFVQQPKGFIRKGEEDKVYRLKK 966
++ + IR+++ + + + Q++VKSAFL+G + EEV+V QP G + D +++LKK
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 967 ALYGLKQALRAWYSRIEAYFVREDFERCPSEHILFTKSKGGRILIVSLYVDDLIFTGNDR 1026
+LYGLKQA RAWY R+ ++ + + R + LF K+ ILIV +YVDD+IF +
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 1027 VMCDEFKSSMMLEFDMSDLGKMKYFLGVE 1055
+C EF M EF+M +G++KYFLG++
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 29.3 bits (64), Expect(2) = 7e-32
Identities = 12/18 (66%), Positives = 15/18 (82%)
Frame = +3
Query: 891 YAQRHEVDYTEVFAPVAR 908
Y+Q+ +DYTE FAPVAR
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
>AV780009
Length = 529
Score = 125 bits (315), Expect = 3e-29
Identities = 63/164 (38%), Positives = 98/164 (59%)
Frame = -1
Query: 1148 AAKRILRYLKGTTDLGIFYKKGGSNMKLMAFPDSDYAGDLDDRRSTSRFVFMLGSGVVSW 1207
AA R+LRY+KG G+F+ S +KL A+ DSD+AG D RRS + + LG+ ++SW
Sbjct: 526 AATRVLRYVKGAPAQGLFFS-ADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISW 350
Query: 1208 SSKKQYVVALSTTEAEYIAAALCACQCVWLIRVLEKIGVEEKTSTEIMCDNSFTIQLSKN 1267
+KKQ V+ S++EAEY A A C+ WL + + + + + CDN + ++ N
Sbjct: 349 RTKKQTTVSRSSSEAEYRALAATVCEVQWLSYLFQFLKLNVPLPVPLFCDNQSALHIAHN 170
Query: 1268 PVFHGKSKHIDVRFHFLRDLVNDGVIKLSYCNSQDQIADIMTKP 1311
P FH ++KHI++ H +R + G+I L ++ Q+ADI TKP
Sbjct: 169 PTFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTKP 38
>AV427921
Length = 284
Score = 120 bits (302), Expect = 1e-27
Identities = 56/94 (59%), Positives = 69/94 (72%)
Frame = +1
Query: 583 RQLTTTYTP*QNGVAERKNRTIMNVVRSMLNEKQVPKVFWSEAVRWCVHIQNRCPIVAVE 642
RQ T YTP QNGV+ERKNRTI+N+VRS+L +PK F EAV W +HI NR P + V+
Sbjct: 1 RQ*TAAYTPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQ 180
Query: 643 NKTPEEAWSGEKPVVHYFRIFECVAHVHVPDQRR 676
N PEEAWSG +P V +FRI C+A+ HVPDQ+R
Sbjct: 181 NMMPEEAWSGRQPAVDHFRISRCLAYAHVPDQKR 282
>BP084332
Length = 368
Score = 104 bits (260), Expect = 8e-23
Identities = 49/106 (46%), Positives = 73/106 (68%)
Frame = +2
Query: 1212 QYVVALSTTEAEYIAAALCACQCVWLIRVLEKIGVEEKTSTEIMCDNSFTIQLSKNPVFH 1271
Q +ALST EAEYI+AA+C+ Q +W+ LE + E ++ I CDN+ I LSKNP+ H
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILE-SNIPIYCDNTAAISLSKNPILH 208
Query: 1272 GKSKHIDVRFHFLRDLVNDGVIKLSYCNSQDQIADIMTKPIKLEQF 1317
++KHI+V++HF+RD V GV+ L + ++ Q ADI TKP+ ++F
Sbjct: 209 SRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRF 346
>BP075943
Length = 547
Score = 97.8 bits (242), Expect = 1e-20
Identities = 62/189 (32%), Positives = 98/189 (51%), Gaps = 12/189 (6%)
Frame = -3
Query: 1004 SKGGRILIVSLYVDDLIFTGNDRVMCDEFKSSMMLEFDMSDLGKMKYFLGVEVKQC*DGI 1063
+ G + LYVDD++ GND KSS+ +F + DLG+ KYFLG+E+ + GI
Sbjct: 545 ASSGSFTALLLYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGI 366
Query: 1064 FIC*RRYARDVLA---------RFDMRDSNAVKNPTVPGTKLSKDEGGVRVDETLFKQVV 1114
+ R+YA +++ RF +N+ T GT L+ D ++++V
Sbjct: 365 VLNQRKYALQLISDSGHFGFQPRFYSHGTNSQTLGTNTGTPLT--------DIGSYRRIV 210
Query: 1115 GSLMYLTVTRPDLMYGVSLISRFMSSPTMSHWLAAKRILRYLKGTTDLGIFYKKGGSNM- 1173
G L+YL TRPD+ + V+ +S+F+S+PT H L+YL G+ G+FY S +
Sbjct: 209 GRLLYLNTTRPDITFAVNQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASSSTL* 30
Query: 1174 --KLMAFPD 1180
L+ PD
Sbjct: 29 LPSLIGGPD 3
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 75.1 bits (183), Expect(2) = 8e-18
Identities = 54/143 (37%), Positives = 76/143 (52%), Gaps = 3/143 (2%)
Frame = -1
Query: 1070 YARDVLARFDMRDSNAVKNPTVPGTKLS--KDEGGVRVDETLFKQVVGSLMYLTVTRPDL 1127
Y D+L +F M +S + T+ G ++ + GG RVD T +K ++GS+ YL R D+
Sbjct: 730 YVDDILKKFKMTNSKYIST-TIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRSDI 554
Query: 1128 MYGVSLISRFMSSPTMSHWLAAKRILRYLKGTTDLGIFYKKGGSNMKLMAFPDSDYAGDL 1187
+ GV L SRFM P H A+R LRY+KGT GIF M ++ +S L
Sbjct: 553 VCGVGLRSRFM-EP*DCH*QGAQRSLRYIKGTLKDGIF-------MLVITM*NS-----L 413
Query: 1188 DDRRST-SRFVFMLGSGVVSWSS 1209
D + T + F LG G +SWSS
Sbjct: 412 DTQIVTRPGYAFHLGRGAISWSS 344
Score = 33.5 bits (75), Expect(2) = 8e-18
Identities = 19/38 (50%), Positives = 22/38 (57%)
Frame = -2
Query: 1205 VSWSSKKQYVVALSTTEAEYIAAALCACQCVWLIRVLE 1242
V + Q VVAL EA YIAA CA Q + L R+LE
Sbjct: 363 VRYHGPLQSVVALYANEA*YIAAESCATQAI*LTRILE 250
>TC10186 weakly similar to UP|Q9FH39 (Q9FH39) Copia-type polyprotein, partial
(4%)
Length = 528
Score = 56.6 bits (135), Expect(2) = 1e-12
Identities = 25/51 (49%), Positives = 35/51 (68%)
Frame = +3
Query: 1276 HIDVRFHFLRDLVNDGVIKLSYCNSQDQIADIMTKPIKLEQFEKLRGMLGV 1326
+I+ ++HFLRD V G I L +C + Q+ADIMTK +K E+F +R ML V
Sbjct: 69 NIETKYHFLRDQVTKGKISLKHCGTDLQVADIMTKGLKTERFRNMRAMLNV 221
Score = 34.7 bits (78), Expect(2) = 1e-12
Identities = 14/22 (63%), Positives = 17/22 (76%)
Frame = +2
Query: 1257 DNSFTIQLSKNPVFHGKSKHID 1278
DN I L+KNPV HG+SKHI+
Sbjct: 5 DNKSAIDLAKNPVSHGRSKHIE 70
>BP057233
Length = 473
Score = 68.9 bits (167), Expect = 5e-12
Identities = 31/73 (42%), Positives = 46/73 (62%)
Frame = -2
Query: 1254 IMCDNSFTIQLSKNPVFHGKSKHIDVRFHFLRDLVNDGVIKLSYCNSQDQIADIMTKPIK 1313
+ CDN L+ NPV H +SKHI++ H++RD V + ++Y + DQIAD +TKP+
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLS 266
Query: 1314 LEQFEKLRGMLGV 1326
+F +LR LGV
Sbjct: 265 HTRFSQLRDKLGV 227
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 65.5 bits (158), Expect = 5e-11
Identities = 29/94 (30%), Positives = 54/94 (56%)
Frame = +1
Query: 1236 WLIRVLEKIGVEEKTSTEIMCDNSFTIQLSKNPVFHGKSKHIDVRFHFLRDLVNDGVIKL 1295
WL +L+ + V + + CDN+ ++ NPVFH ++KHI++ H +R+ + G+I L
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 1296 SYCNSQDQIADIMTKPIKLEQFEKLRGMLGVTEV 1329
+S + +ADI TK + + F ++ LG+ +
Sbjct: 184 LPISSSEPLADIYTKALSPQNFHQICAKLGLINI 285
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 64.7 bits (156), Expect = 9e-11
Identities = 41/146 (28%), Positives = 71/146 (48%), Gaps = 9/146 (6%)
Frame = +2
Query: 983 EAYFVREDFERCPSEHILFTKS-KGGRILIVSLYVDDLIFTGNDRVMCDEFKSSMMLEFD 1041
+++ + + R S+H + K +I+ LYVDD++ G ++ E K+ + EFD
Sbjct: 5 DSFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREFD 184
Query: 1042 MSDLGKMKYFLGVEVKQC*DG--IFIC*RRYARDVLARFDMRDSNAVKNPTVPGTKL--- 1096
M DLG LG+++ + I++ + Y + VL RF+M+D N + P KL
Sbjct: 185 MKDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSSS 364
Query: 1097 ---SKDEGGVRVDETLFKQVVGSLMY 1119
S + + + + VGSLMY
Sbjct: 365 MIPSSEAERMEMSRVPYASAVGSLMY 442
>AV424544
Length = 276
Score = 63.5 bits (153), Expect = 2e-10
Identities = 31/91 (34%), Positives = 53/91 (58%)
Frame = +3
Query: 980 SRIEAYFVREDFERCPSEHILFTKSKGGRILIVSLYVDDLIFTGNDRVMCDEFKSSMMLE 1039
+++ +Y + + +H LFTK + ++ +YVDDLI GND K+ + ++
Sbjct: 3 AKLSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQ 182
Query: 1040 FDMSDLGKMKYFLGVEVKQC*DGIFIC*RRY 1070
F + DLG +KYFLG+EV + G+F+ R+Y
Sbjct: 183 FRIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 63.2 bits (152), Expect = 3e-10
Identities = 31/98 (31%), Positives = 54/98 (54%)
Frame = +1
Query: 1232 CQCVWLIRVLEKIGVEEKTSTEIMCDNSFTIQLSKNPVFHGKSKHIDVRFHFLRDLVNDG 1291
C+ WL +L+ + V + + + CD+ ++ N VFH ++KH+D+ H +R+ +
Sbjct: 1 CEL*WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAK 180
Query: 1292 VIKLSYCNSQDQIADIMTKPIKLEQFEKLRGMLGVTEV 1329
+ L +S DQ ADI+TKP++ F L LGV +
Sbjct: 181 LFHLLPISSVDQTADILTKPLESGPFSHLVSKLGVLNI 294
>AU088980
Length = 360
Score = 62.4 bits (150), Expect = 5e-10
Identities = 28/97 (28%), Positives = 53/97 (53%)
Frame = +2
Query: 594 NGVAERKNRTIMNVVRSMLNEKQVPKVFWSEAVRWCVHIQNRCPIVAVENKTPEEAWSGE 653
N + ERK+R I+NV R+++ +PK W+ AV+ ++ N P ++ + P + + +
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 654 KPVVHYFRIFECVAHVHVPDQRRSKLDDKSKKCVFLG 690
P + ++F + + KLD +++KCVFLG
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLG 292
>BP043850
Length = 515
Score = 61.6 bits (148), Expect = 8e-10
Identities = 31/96 (32%), Positives = 56/96 (58%)
Frame = -1
Query: 1233 QCVWLIRVLEKIGVEEKTSTEIMCDNSFTIQLSKNPVFHGKSKHIDVRFHFLRDLVNDGV 1292
+ +WL +L ++G + T + DN+ IQ++ NPV+H ++HI+V H +R+ + V
Sbjct: 512 EIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRRV 333
Query: 1293 IKLSYCNSQDQIADIMTKPIKLEQFEKLRGMLGVTE 1328
I L + ++ QIADI+TK + ++ L L + E
Sbjct: 332 ITLPHVSTSVQIADILTKSLTRQRHNFLVSKLMLVE 225
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 55.5 bits (132), Expect = 6e-08
Identities = 26/81 (32%), Positives = 44/81 (54%)
Frame = +3
Query: 1232 CQCVWLIRVLEKIGVEEKTSTEIMCDNSFTIQLSKNPVFHGKSKHIDVRFHFLRDLVNDG 1291
C+ +WL L + + I CDN + L+ N VFH ++++I++ H + V G
Sbjct: 18 CEALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFG 197
Query: 1292 VIKLSYCNSQDQIADIMTKPI 1312
++ L + S DQ+AD+ TK I
Sbjct: 198 ILHLLHVPSSDQVADVFTKTI 260
>BP063694
Length = 511
Score = 53.5 bits (127), Expect = 2e-07
Identities = 36/113 (31%), Positives = 54/113 (46%), Gaps = 3/113 (2%)
Frame = -2
Query: 435 HCRFGHLNHKGLRTLSHKKMVVGLPSLESPEKICTTCLTGKQHREPVPKRSLWRASKQLQ 494
H R GH+N ++ L HK + + S+ CT+C K R + RAS L
Sbjct: 354 HSRLGHVNFDIIKQL-HKHGCLDVSSILPKPICCTSCQMAKSKRLVFHDNNK-RASAVLD 181
Query: 495 LVHSDICGPIKPSWNSDKRYILSFIDDHTRKTWVYFLHEK---SEAFVKFKEY 544
L+H D+ GP + Y + F+DD +R TW Y L K S+ ++FK +
Sbjct: 180 LIHCDLRGPSPVASIDGFSYFVIFVDDFSRFTWFYPLKRKSDFSDVLLRFKVF 22
>BP033602
Length = 533
Score = 52.8 bits (125), Expect = 4e-07
Identities = 34/90 (37%), Positives = 46/90 (50%), Gaps = 1/90 (1%)
Frame = +3
Query: 435 HCRFGHLNHKGLRTLSHKKMV-VGLPSLESPEKICTTCLTGKQHREPVPKRSLWRASKQL 493
H R GH N + LR L V SLE C +C+ K R P + + AS+
Sbjct: 282 HNRLGHPNFQYLRHLFPDLFKNVNCSSLE-----CESCVLAKNQRAPYYSQP-YHASRPF 443
Query: 494 QLVHSDICGPIKPSWNSDKRYILSFIDDHT 523
L+HSD+ GP K + KR+ ++FIDDHT
Sbjct: 444 YLIHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 51.6 bits (122), Expect = 8e-07
Identities = 40/127 (31%), Positives = 63/127 (49%), Gaps = 6/127 (4%)
Frame = +3
Query: 1203 GVVSWSSKKQYVVALSTTEAEYIAAALCACQCVWLIRVLEKIGVEEKTSTEIMCDNSFT- 1261
G V+W S+ Q VALST EAE+IAA + +W+ L+ S I+C T
Sbjct: 21 GAVAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWFH---SHPILCCIVITK 191
Query: 1262 --IQLSKNPVFHGKSKHIDVRFHFLRDLVNDGVIKLSYCNSQDQIADIMTKPI---KLEQ 1316
L++ +F + LRD++N +++L ++ D AD+MTK + KLE
Sbjct: 192 ALFTLARILLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLPREKLEV 371
Query: 1317 FEKLRGM 1323
+ + GM
Sbjct: 372 CDMIAGM 392
>AV410378
Length = 358
Score = 51.6 bits (122), Expect = 8e-07
Identities = 36/115 (31%), Positives = 52/115 (44%), Gaps = 28/115 (24%)
Frame = +1
Query: 771 LPNEPISNTPEELVEGRVVRERRVPSWFADYE-------------TREGLEENLNA---- 813
LP+E +SN E V+ R RR PS+ DY + G+ L+
Sbjct: 4 LPSETLSNPEEGNVQRISNRVRRPPSYLQDYHCTLAASTTVSKVPSSAGISYPLSKVISY 183
Query: 814 -----------MMMVTENDPVSFGEAVKNKKWRDAMSAEIESIERNQSWELTVLP 857
M + T ++P + EAVK++ WR AM EIE++ERN +W L P
Sbjct: 184 HKLTPSYRAFIMNITTVSEPTRYSEAVKHECWRKAMDQEIEALERNHTWILVDKP 348
>BP052632
Length = 489
Score = 51.2 bits (121), Expect = 1e-06
Identities = 38/137 (27%), Positives = 60/137 (43%)
Frame = -3
Query: 644 KTPEEAWSGEKPVVHYFRIFECVAHVHVPDQRRSKLDDKSKKCVFLGVSDESKAWRLYVP 703
KTP E W G +P V YF F C + + K D+K K + LG S SKA+ ++
Sbjct: 487 KTPYELWRGRRPTVSYFHPFGCK*FILNTNDNIGKFDNKFDKRILLGYSSNSKAYIVFNS 308
Query: 704 VSKKIIVSKDVVFEEEESWDWGRIEEEIKLDILECGEEDQNEEENGRTDLNNLSSNSSSS 763
++ + S +V F++ R+E+ D L DQ+EE + +N
Sbjct: 307 RTQVVEESINVKFDD*SIEATYRLEK----DFLNLDLSDQDEE---------VPTNEGQP 167
Query: 764 SNSLPESLPNEPISNTP 780
S + E +P + P
Sbjct: 166 SETSHEEPSTQPQTTNP 116
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.136 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,981,557
Number of Sequences: 28460
Number of extensions: 300497
Number of successful extensions: 2073
Number of sequences better than 10.0: 102
Number of HSP's better than 10.0 without gapping: 1862
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1986
length of query: 1332
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1231
effective length of database: 2,023,140
effective search space: 2490485340
effective search space used: 2490485340
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0346.12