BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0340.11
(420 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15067 40 0.001
TC9596 similar to UP|AAR24722 (AAR24722) At5g49400, partial (66%) 33 0.11
AV771192 28 2.8
TC10048 similar to UP|Q9M7N4 (Q9M7N4) MFP1 attachment factor 1, ... 28 2.8
TC13916 homologue to UP|Q9FK48 (Q9FK48) Similarity to transcript... 28 3.7
TC12346 similar to GB|AAA32792.1|432446|ATHFUS6A FUS6 {Arabidops... 28 3.7
TC14766 similar to UP|Q7XXR8 (Q7XXR8) Nascent polypeptide associ... 28 3.7
TC8351 homologue to UP|Q41399 (Q41399) Chalcone reductase, complete 28 3.7
AV411385 27 4.8
TC12653 similar to UP|BIOB_ARATH (P54967) Biotin synthase (Biot... 27 8.2
AV423544 27 8.2
TC9175 weakly similar to UP|INAD_DROME (Q24008) Inactivation-no-... 27 8.2
BP063872 27 8.2
AI967683 27 8.2
TC14500 UP|Q9S7B1 (Q9S7B1) Nodule INCEPTION protein, complete 27 8.2
AV420631 27 8.2
>TC15067
Length = 542
Score = 39.7 bits (91), Expect = 0.001
Identities = 20/30 (66%), Positives = 22/30 (72%)
Frame = +3
Query: 123 HSAETRERIKRRTSEALREPKVRKKMAEHP 152
H + RI RTS+ALRE KVRKKMAEHP
Sbjct: 69 HFSLISTRIN*RTSKALRESKVRKKMAEHP 158
>TC9596 similar to UP|AAR24722 (AAR24722) At5g49400, partial (66%)
Length = 1196
Score = 32.7 bits (73), Expect = 0.11
Identities = 32/128 (25%), Positives = 52/128 (40%), Gaps = 10/128 (7%)
Frame = +2
Query: 58 DDDSDNQSE----------TSVDSSDYLNGGDGVVGLSDSLEIESLSRKAVKERMRRFKI 107
D DS + SE +SV SDY +G SDS E + R R+ K
Sbjct: 644 DSDSGSDSEDSVFETESGSSSVTGSDYSSGSSSDSSSSDSEE--------ERRRRRKKKT 799
Query: 108 GLANKGRVPWNKGRKHSAETRERIKRRTSEALREPKVRKKMAEHPRFHSDKIKEKISYSL 167
KGR + +K+S+ + S++ + K ++ H R H ++K+ S
Sbjct: 800 QKKKKGR----RHKKYSSSSESSDSESASDSDSDDKSSRRKRRHSRRH*IRLKDVRSSYQ 967
Query: 168 RRVWQERL 175
W ++L
Sbjct: 968 FGEWVDKL 991
>AV771192
Length = 487
Score = 28.1 bits (61), Expect = 2.8
Identities = 11/13 (84%), Positives = 12/13 (91%)
Frame = -3
Query: 21 CTFCPPTTLQVKT 33
CTF PPTTLQVK+
Sbjct: 485 CTFFPPTTLQVKS 447
>TC10048 similar to UP|Q9M7N4 (Q9M7N4) MFP1 attachment factor 1, partial
(66%)
Length = 718
Score = 28.1 bits (61), Expect = 2.8
Identities = 21/89 (23%), Positives = 36/89 (39%)
Frame = +1
Query: 14 TNVVGCACTFCPPTTLQVKTATPRLVFVCSLQRVLSIEELQINVDDDSDNQSETSVDSSD 73
TN+ G + + PPT RL+ S VLS + D+ S + ++
Sbjct: 124 TNLSGISFSIWPPTQRTRDAVINRLIETLSTPSVLSKRYGTMPADEAS--AAARQIEDEA 297
Query: 74 YLNGGDGVVGLSDSLEIESLSRKAVKERM 102
+ G +D +EI + K + +RM
Sbjct: 298 FAAAGGSAAADTDGIEILQVYSKEISKRM 384
>TC13916 homologue to UP|Q9FK48 (Q9FK48) Similarity to transcription
regulator, partial (17%)
Length = 603
Score = 27.7 bits (60), Expect = 3.7
Identities = 34/136 (25%), Positives = 57/136 (41%), Gaps = 1/136 (0%)
Frame = +2
Query: 192 IANAAKKGGCGQEELDWDSYNKIKEQLELQQLLQAEEKEKEKLMAIARAEKFIQSWSECL 251
I KK G E D +NK+ + Q + E K+++ + R I++W +
Sbjct: 185 IDRVLKKVQEGVEVFD-SIWNKVYDTDNANQKEKFEADLKKEIKKLQRYRDQIKTWIQSS 361
Query: 252 AKEAKKGGSGEKELDWDSYEKIQEEMFLLNQIRRTTEKAKAKERARTKAEKEARIKAIKK 311
+ KK + ++ D+ + I+ EM E+ K EKE + KA K
Sbjct: 362 EIKDKKVSASYEQALVDARKLIEREM----------ERFKI-------CEKETKTKAFSK 490
Query: 312 VMLNQK-KKDLRERIK 326
L Q+ K D +E+ K
Sbjct: 491 EGLGQQPKTDPKEKAK 538
>TC12346 similar to GB|AAA32792.1|432446|ATHFUS6A FUS6 {Arabidopsis
thaliana;} , partial (21%)
Length = 434
Score = 27.7 bits (60), Expect = 3.7
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +3
Query: 50 IEELQINVDDDSDNQSETSVDSSDYLN 76
I+E+ N DDSDN+S ++ S D L+
Sbjct: 72 IDEIYANGGDDSDNRSRRAIMSGDQLD 152
>TC14766 similar to UP|Q7XXR8 (Q7XXR8) Nascent polypeptide associated
complex alpha chain, partial (58%)
Length = 600
Score = 27.7 bits (60), Expect = 3.7
Identities = 19/83 (22%), Positives = 34/83 (40%), Gaps = 1/83 (1%)
Frame = +2
Query: 17 VGCACTFCPPTTLQVKTATPRLVFVCSLQRVLSIEELQINVDDDSDNQSETSVDSSDYLN 76
+ CT +T + L+ Q+ + +E + DD+ D+ + + D +
Sbjct: 20 ISSVCTGTHRSTAMTAQSQEELLAAHLEQQKIHDDEPVVEDDDEDDDDEDDDDEDDDNIE 199
Query: 77 GGDG-VVGLSDSLEIESLSRKAV 98
G +G G S E SRKA+
Sbjct: 200 GQEGDASGRSKQTRSEKKSRKAM 268
>TC8351 homologue to UP|Q41399 (Q41399) Chalcone reductase, complete
Length = 1331
Score = 27.7 bits (60), Expect = 3.7
Identities = 27/69 (39%), Positives = 31/69 (44%), Gaps = 4/69 (5%)
Frame = +2
Query: 108 GLANKGRVPWNKGRKHSAETRERIKRRTSEALREPKVRKKMAEHPRFHS-DKIKEKISY- 165
G NKG+VPW K A TR R RT + L V+K RFHS EK Y
Sbjct: 791 GCTNKGQVPWLK-----ASTR-RG*NRTLKYLILS*VKKNWRRLSRFHSAGNT*EKCGYL 952
Query: 166 --SLRRVWQ 172
L R W+
Sbjct: 953 KMGLARPWK 979
>AV411385
Length = 416
Score = 27.3 bits (59), Expect = 4.8
Identities = 13/46 (28%), Positives = 21/46 (45%)
Frame = -1
Query: 200 GCGQEELDWDSYNKIKEQLELQQLLQAEEKEKEKLMAIARAEKFIQ 245
G G+ E++W+ +K + + L+ EEK I FIQ
Sbjct: 278 GLGKLEINWNELQNVKRMMPAMKRLKYEEKRNFSNTIIRLVAAFIQ 141
>TC12653 similar to UP|BIOB_ARATH (P54967) Biotin synthase (Biotin
synthetase) , partial (29%)
Length = 535
Score = 26.6 bits (57), Expect = 8.2
Identities = 15/54 (27%), Positives = 26/54 (47%), Gaps = 6/54 (11%)
Frame = +1
Query: 185 FLSWEQCIANAAKKGGCGQ------EELDWDSYNKIKEQLELQQLLQAEEKEKE 232
F +QC + K GGC + + +D+ K + L+ + ++QA K KE
Sbjct: 358 FREVQQCTLLSVKTGGCSEDCSYCPQSSRYDTGLKAQRLLDKEAVIQAANKAKE 519
>AV423544
Length = 443
Score = 26.6 bits (57), Expect = 8.2
Identities = 17/52 (32%), Positives = 27/52 (51%)
Frame = +3
Query: 207 DWDSYNKIKEQLELQQLLQAEEKEKEKLMAIARAEKFIQSWSECLAKEAKKG 258
DW+ Y + +EQ + + KEK +L + ++ KF ECLA +K G
Sbjct: 258 DWNPYIQTEEQHRAYSIHLSL*KEKIRL*SP*QS*KFPNYSFECLASSSKGG 413
>TC9175 weakly similar to UP|INAD_DROME (Q24008)
Inactivation-no-after-potential D protein, partial (5%)
Length = 692
Score = 26.6 bits (57), Expect = 8.2
Identities = 25/110 (22%), Positives = 47/110 (42%)
Frame = +3
Query: 92 SLSRKAVKERMRRFKIGLANKGRVPWNKGRKHSAETRERIKRRTSEALREPKVRKKMAEH 151
++S + + R F N+ R K +++ + R +KRR ++ K RKK
Sbjct: 12 NMSTDEPRSKPR*FPNQNLNQRRKKKVKKKRNLQQRRVNLKRRKKRRKQQRKPRKKRV-- 185
Query: 152 PRFHSDKIKEKISYSLRRVWQERLKSKRFREQIFLSWEQCIANAAKKGGC 201
+K+++ +RV ++ K +R + I W N A +G C
Sbjct: 186 -------VKKRMITKKKRVVKKGKKKERKKRTITGQWLTWKRN*A*RG*C 314
>BP063872
Length = 445
Score = 26.6 bits (57), Expect = 8.2
Identities = 23/82 (28%), Positives = 40/82 (48%)
Frame = +3
Query: 204 EELDWDSYNKIKEQLELQQLLQAEEKEKEKLMAIARAEKFIQSWSECLAKEAKKGGSGEK 263
EELD + N E +Q + EE E+ L + + EK I++ ++
Sbjct: 222 EELD-KAVNAQMEMFIMQNCV--EELEQMNLALLTKCEKHIEA--------SRFSDKVIS 368
Query: 264 ELDWDSYEKIQEEMFLLNQIRR 285
EL+ ++ ++ E FLL+QIR+
Sbjct: 369 ELETENLMQLMAEEFLLHQIRK 434
>AI967683
Length = 464
Score = 26.6 bits (57), Expect = 8.2
Identities = 13/47 (27%), Positives = 25/47 (52%)
Frame = +3
Query: 48 LSIEELQINVDDDSDNQSETSVDSSDYLNGGDGVVGLSDSLEIESLS 94
L +++ + +D DN+ DSS Y + + ++G S+S + E S
Sbjct: 57 LYTQDISDDESEDDDNEGSAHDDSSAYSSEPEELLGSSESSDSEETS 197
>TC14500 UP|Q9S7B1 (Q9S7B1) Nodule INCEPTION protein, complete
Length = 3001
Score = 26.6 bits (57), Expect = 8.2
Identities = 26/117 (22%), Positives = 49/117 (41%), Gaps = 1/117 (0%)
Frame = +3
Query: 274 QEEMFLLNQIRRTTEKAKAKERARTKAEKEARIKAIKKVMLNQKKKDLRERIKGRGNIKS 333
QE + R ++ K+ E+ RTKAEK ++ +++ L++ K G +
Sbjct: 1743 QESSYTFGSRRSSSGGRKSGEKRRTKAEKTISLQVLRQYFAG----SLKDAAKSIGVCPT 1910
Query: 334 QLCKNANEDGEAALEITQQFKLDTKLTKINISKNINSEVAREGGFS-NSIYPTYNKL 389
L + + G + K+ L K+ + I+S EG S Y ++ +L
Sbjct: 1911 TLKRICRQHGITRWPSRKIKKVGHSLKKLQLV--IDSVQGAEGAIQIGSFYASFPEL 2075
>AV420631
Length = 355
Score = 26.6 bits (57), Expect = 8.2
Identities = 14/26 (53%), Positives = 15/26 (56%)
Frame = +1
Query: 286 TTEKAKAKERARTKAEKEARIKAIKK 311
T EKAKA+E A AEK A KK
Sbjct: 265 TLEKAKAEEEAIVAAEKAREASATKK 342
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.130 0.358
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,131,022
Number of Sequences: 28460
Number of extensions: 72645
Number of successful extensions: 375
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 374
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 374
length of query: 420
length of database: 4,897,600
effective HSP length: 93
effective length of query: 327
effective length of database: 2,250,820
effective search space: 736018140
effective search space used: 736018140
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0340.11